当前位置： SCI文献检索 > JOURNAL OF IMMUNOLOGICAL METHODS期刊下所有文献 > A potential radioimmunoassay system for detection of Hanganutziu-Deicher type heterophile antigen(s) and antibodies in tissues and fluids.

A potential radioimmunoassay system for detection of Hanganutziu-Deicher type heterophile antigen(s) and antibodies in tissues and fluids.

Abstract：

:A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein previously found to have a strong H-D antigen potency. The antigen-antibody complex was precipitated with normal human serum as the carrier protein, followed by the addition of rabbit anti-human IgG F(ab')2 serum. With this method, different H-D antigen-active molecules were compared for heterophile H-D antigen potency with reasonable sensitivity detecting about 0.3 ng of cold glycoprotein. 8 different lung cancer tissues were assayed for H-D antigen. The sera from the 8 lung cancer patients were also screened by ELISA and RIA in an attempt to correlate expression of H-D antigen on tissues with elevation of H-D antibodies. The results showed that all patients' tissues expressed the antigen(s) but only 3 of them had abnormal levels of H-D antibodies. This could have been due to excess antigens in circulation or immune complexes.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Mukuria JC,Naiki M,Hashimoto M,Nishiura K,Okabe M,Kato S

doi

10.1016/0022-1759(85)90168-1

subject

Has Abstract

pub_date

1985-06-12 00:00:00

pages

97-106

issue

1

eissn

0022-1759

issn

1872-7905

pii

0022-1759(85)90168-1

journal_volume

80

pub_type

杂志文章

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