A simple method for quantifying IgM of low molecular weight.

abstract：:Formation of rosettes with sheep RBC by blood lymphocytes from young pigs was increased from 24.4% +/- 2.2 (mean +/- S.E.) in PBS to 54.1% +/- 1.9 in the presence of dextran. This increase was achieved without inducing appreciable rosette formation with other RBC which do not form rosettes in PBS. Lymphocytes which r...

journal_title：Journal of immunological methods

authors： Binns RM

更新日期：1978-01-01 00:00:00

abstract：:Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach tha...

journal_title：Journal of immunological methods

authors： Dzutsev AK,Belyakov IM,Isakov DV,Gagnon SJ,Margulies DH,Berzofsky JA

更新日期：2008-04-20 00:00:00

abstract：:A number of supravital fluorochromes are available to study leucocyte functions in vitro and in vivo. The fluorescein ester most widely used, fluorescein diacetate, has the disadvantage of rapid cellular efflux, whereas more recently developed fluorescent probes do not exhibit this inconvenient trait. However, their e...

journal_title：Journal of immunological methods

authors： De Clerck LS,Bridts CH,Mertens AM,Moens MM,Stevens WJ

更新日期：1994-06-03 00:00:00

abstract：:A new compact and cell dense continuous culture system was developed. The system consisted of a culture bag, which had two compartments separated by a semipermeable membrane, and an external rotator. Cells were cultured in the inner compartment with medium containing serum or the necessary growth factors. The outer co...

journal_title：Journal of immunological methods

authors： Nakamura Y,Watanabe K,Noto T,Tajima T,Yamamura M

更新日期：1989-03-10 00:00:00

abstract：:A continuous cell-line has been shown to be adaptable to preparation of supernatants containing lymphokine activities. Separation and partial purification of migration inhibiting, colony stimulating and interferon activities from such preparations has been achieved by the use of density gradient iso-electric focusing....

journal_title：Journal of immunological methods

authors： Trudgett A

更新日期：1976-01-01 00:00:00

abstract：:Helicobacter pylori is a bacterial pathogen, estimated to infect half the world's population. The bacterium is the aetiological cause of gastritis, the common precursor for peptic ulcer disease and gastric cancer. Immunisation of at-risk individuals is the most cost-effective means of dealing with such a widespread pa...

journal_title：Journal of immunological methods

authors： Keenan JI,Allardyce RA,Bagshaw PF

更新日期：1997-11-10 00:00:00

abstract：:Among the many methods currently available for quantifying mRNA transcript abundance, reverse transcription-polymerase chain reaction (RT-PCR) has proved to be the most sensitive. Recently, several protocols for real-time relative RT-PCR using the reporter dye SYBR Green I have appeared in the literature. In these met...

journal_title：Journal of immunological methods

authors： Marino JH,Cook P,Miller KS

更新日期：2003-12-01 00:00:00

abstract：:Human peripheral blood or tonsil lymphocytes produce T cell growth factor (TCGF), when activated with neuraminidase (NA) and galactose oxidase (GO). Partial purification of NAGO-TCGF on Sepharose G-100 columns gave a TCGF-active fraction within the same molecular weight range as the conventional lectin-induced TCGF (a...

journal_title：Journal of immunological methods

authors： Berzins T,Wikén M,Hellström U,Perlmann P

更新日期：1983-10-28 00:00:00

abstract：:We have developed a competitive enzyme immunoassay suitable for routine monitoring of intracellular levels of 5'-monophosphate-AZT (AZT-MP). This assay is performed in 96-well microtiter plates coated with anti-rabbit immunoglobulin antibodies and is based on the use of rabbit polyclonal antibodies raised against an A...

journal_title：Journal of immunological methods

authors： Goujon L,Brossette T,Dereudre-Bosquet N,Creminon C,Clayette P,Dormont D,Mioskowski C,Lebeau L,Grassi J

更新日期：1998-09-01 00:00:00

abstract：:Covalently cross-linked large and intermediate-sized IgA oligomers, prepared with IgA anti-dinitrophenyl (DNP) and bis-DNP-pimelic acid ester, were used to examine the ability of different concentrations (3.5%, 5% and 7%, w/v) of polyethylene glycol (PEG) to precipitate IgA immune complexes (IgA-IC). The size of the I...

journal_title：Journal of immunological methods

authors： Imai H,Chen A,Wyatt RJ,Rifai A

更新日期：1987-11-05 00:00:00

abstract：:Hybridoma cells producing monoclonal antibodies against tumor-shed antigens were generated by fusing mouse myeloma cells with spleen cells from immunosuppressed mice bearing human melanoma xenografts. Thirty-eight fusion experiments were performed at different stages of tumor growth. Hybridomas producing anti-melanoma...

journal_title：Journal of immunological methods

authors： Herlyn D,Atkinson B,Koprowski H

更新日期：1983-02-25 00:00:00

abstract：:We describe a simple and efficient non-chromatographic method for the purification of murine IgG3 and IgM monoclonal antibodies (MAbs) which takes advantage of their euglobulin properties. Following filtration, ascitic fluid is dialysed against demineralized water and centrifuged at 22,000 X g for 30 min. The resultin...

journal_title：Journal of immunological methods

authors： García-González M,Bettinger S,Ott S,Olivier P,Kadouche J,Pouletty P

更新日期：1988-06-28 00:00:00

abstract：:The majority of vaccines and several treatments are administered by intramuscular injection. The aim is to engage and activate immune cells, although they are rare in normal skeletal muscle. The phenotype and function of resident as well as infiltrating immune cells in the muscle after injection are largely unknown. W...

journal_title：Journal of immunological methods

authors： Liang F,Ploquin A,Hernández JD,Fausther-Bovendo H,Lindgren G,Stanley D,Martinez AS,Brenchley JM,Koup RA,Loré K,Sullivan NJ

更新日期：2015-10-01 00:00:00

abstract：:Early pregnancy factor (EPF) is a secreted protein with growth regulatory and immunomodulatory properties. It functions as an autocrine growth factor for tumour cells and as an autocrine or paracrine growth factor for regenerating normal cells. Anti-EPF antibodies have demonstrable anti-tumour activity and, as a resul...

journal_title：Journal of immunological methods

authors： Hammond F,Cavanagh A,Morton H,Hillyard N,Papaioannou A,Clark M,Wanigesekera D,Swanton M,Ward R

更新日期：2000-10-20 00:00:00

abstract：:The Rho family GTPase Cdc42 is a critical regulator of cellular polarization from yeast to man. An analysis of its function in T cell activation is therefore of interest. This analysis poses two substantial challenges, similar to the analysis of many other critical T cell signaling intermediates. First, Cdc42 is requi...

journal_title：Journal of immunological methods

authors： Tskvitaria-Fuller I,Mistry N,Sun S,Wülfing C

更新日期：2007-01-30 00:00:00

abstract：:A method is described to identify specific antibody-forming cells and their immunoglobulin classes by a multilayer cluster formation. Cells producing specific antibodies are mixed with erythrocytes coated with the corresponding antigen in presence of rabbit anti-human immunoglobulins. Clusters are formed only if the m...

journal_title：Journal of immunological methods

authors： Steinitz M

更新日期：1980-01-01 00:00:00

abstract：:Limiting dilution (LD) cultures are often used to study cellular heterogeneity in responses of murine splenocytes to specific or polyclonal activation. LD titration curves often reveal a nonlinear dependence of response on input cell dose. Although 'zigzag' shaped curves of this kind are often interpreted and analyzed...

journal_title：Journal of immunological methods

authors： Dozmorov IM,Lutsenko GV,Sidorov LA,Miller RA

更新日期：1996-02-05 00:00:00

abstract：:In general, antigen affinity columns made with commercially prepared activated affinity supports bind antibody specific for the coupled antigen. Nonetheless, in some cases affinity purification may yield antibodies to molecules other than the molecule of interest. In this report, we demonstrate such an occurrence: an ...

journal_title：Journal of immunological methods

authors： Sweet SC,Gnegy ME,Welsh MJ

更新日期：1991-01-24 00:00:00

abstract：:In developing countries the acquisition and use of radioisotopes with their short shelf lives poses enormous problems. The objective of this study was to develop a simple, sensitive, non-isotopic method for the measurement of TSH in human serum. A conventional sandwich enzyme-linked immunosorbent assay (ELISA) techniq...

journal_title：Journal of immunological methods

authors： Ohwovoriole AE,Wilkin TJ

更新日期：1988-11-25 00:00:00

abstract：BACKGROUND:ABO subgroups or weaker variants of A or B are group A or B subjects whose erythrocytes give a weak or negative reaction serologically with anti-A or Anti - B antisera respectively. Occurrence of these subgroups may lead to an ABO discrepancy which often puts transfusion services in a quandary. ABO subgroups...

journal_title：Journal of immunological methods

authors： Tiwari AK,Setya D,Arora D,Mehta SP,Aggarwal G,Mitra S

更新日期：2020-12-01 00:00:00

abstract：:Investigation of MHC class I endogenous antigen processing and presentation is important for understanding non-self immune recognition, vaccine design, immune evasion by pathogens and tumours, and elucidation of the mechanisms of certain auto-immune diseases. To study the MHC class I antigen-processing pathway, the pr...

journal_title：Journal of immunological methods

authors： Fu H,Ding J,Flutter B,Gao B

更新日期：2008-06-01 00:00:00

abstract：:Two different methods have been used to study immune responses in the rat to Streptococcus pneumoniae type 3 and type 4 capsular polysaccharides (PPS). First, for simultaneous detection of the specificity and isotype of anti-PPS antibody-containing cells (ACC) in cryostat sections of lymphoid tissue, a double immunocy...

journal_title：Journal of immunological methods

authors： van den Dobbelsteen GP,van Rooijen N,Sminia T,van Rees EP

更新日期：1991-12-15 00:00:00

abstract：:Giant liposomes (mean diameter 5.5 microns) composed of egg phosphatidylcholine or distearoyl phosphatidylcholine, phosphatidyl glycerol, cholesterol and triolein were prepared by a double emulsion technique. They were then mixed with model particulate (killed Bacillus subtilis, and killed Bacille Calmette-Guérin) and...

journal_title：Journal of immunological methods

authors： Antimisiaris SG,Jayasekera P,Gregoriadis G

更新日期：1993-12-03 00:00:00

abstract：:The usefulness of cryopreserved sheep erythrocytes (unsensitized or coated with fluorescein, IgG-type antibody or complement) for marker tests on human blood lymphocytes was studied. It appears that the rosetting properties or freshly prepared or frozen-stored erythrocytes are not significantly different. The method m...

journal_title：Journal of immunological methods

authors： Gmelig-Meyling F,van der Ham M,Ballieux RE

更新日期：1976-01-01 00:00:00

abstract：:The structural diversity of the immune response may be considerably restricted by the structure of the hapten used to elicit catalytic antibodies. The ligand-binding mode and the shapes of the binding pockets of hydrolytic antibodies induced to different transition-state analogs that contain an unsubstituted arylphosp...

journal_title：Journal of immunological methods

authors： Golinelli-Pimpaneau B

更新日期：2002-11-01 00:00:00

abstract：:We found that some plasma proteins such as haptoglobin (Mr 200,000) and alpha 1-acid glycoprotein (Mr 40,000) do not bind at all to nitrocellulose membranes in classical transfer conditions. In contrast, these proteins can be clearly identified when a charged nylon membrane is disposed on the anodal side of the nitroc...

journal_title：Journal of immunological methods

authors： Miribel L,Arnaud P

更新日期：1988-03-16 00:00:00

abstract：:A recombinant retroviral vector with an inserted bacterial neomycin resistance (neo) gene was used to transfer in vitro neomycin resistance (neoR) into murine cytotoxic lymphocyte precursors (CLP). The infection protocol involved co-cultivation of mitogen-activated splenic T-blasts with irradiated cells that produced ...

journal_title：Journal of immunological methods

authors： Reimann J,Heeg K,Wagner H,Keller G,Wagner EF

更新日期：1986-05-01 00:00:00

abstract：:RIA and ELISA were compared for their ability to detect IgE in different rodent species. With a sheep anti-rat IgE antibody good correlation (P less than 0.001) between the 2 assay methods for IgE was found in rats. RIA failed to detect the IgE of Mastomys natalensis while ELISA proved to be a suitable test. However, ...

journal_title：Journal of immunological methods

authors： Reiner G,Haque A,Zahner H

更新日期：1984-04-13 00:00:00

abstract：:Cow's milk protein allergy (CMPA) is one of the most prevalent human food-borne allergies, particularly in children. Experimental animal models have become critical tools with which to perform research on new therapeutic approaches and on the molecular mechanisms involved. However, oral food allergen sensitization in ...

journal_title：Journal of immunological methods

authors： Bailón E,Cueto-Sola M,Utrilla P,Rodríguez-Ruiz J,Garrido-Mesa N,Zarzuelo A,Xaus J,Gálvez J,Comalada M

更新日期：2012-07-31 00:00:00

abstract：:Although many different protocols for transfection of lymphoid cell lines exist, successful DNA transfer into primary B lymphocytes has, to date, not been demonstrated. We now describe a simple method for gene transfer into highly purified normal and malignant B lymphocytes by electroporation. Using a powerful express...

journal_title：Journal of immunological methods

authors： Buschle M,Brenner MK,Chen IS,Drexler HG,Gignac SM,Rooney CM

更新日期：1990-10-04 00:00:00