Abstract:
:In developing countries the acquisition and use of radioisotopes with their short shelf lives poses enormous problems. The objective of this study was to develop a simple, sensitive, non-isotopic method for the measurement of TSH in human serum. A conventional sandwich enzyme-linked immunosorbent assay (ELISA) technique was modified to incorporate the avidin-biotin complex (ABC) in order to enhance the sensitivity of a solid-phase micro-ELISA for TSH. The lower limit of TSH detectability was 0.2 mIU/l. TSH levels ranged from 0.3 to 5.1 mIU/l in euthyroid subjects, 11.5-98.0 mIU/l in hypothyroid subjects and less than 0.2-2.1 mIU/l in hyperthyroid subjects. Sera from euthyroid post-menopausal or pregnant women yielded TSH levels within the normal range. The correlation between the TSH values obtained in the ELISA-ABC and a sensitive commercial immunoradiometric method was high (n = 59, r = 0.980, P less than 0.01). The simplicity and relatively high sensitivity of the procedure should make it a method of choice for TSH determination in small hospitals and those in developing countries.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Ohwovoriole AE,Wilkin TJdoi
10.1016/0022-1759(88)90315-8subject
Has Abstractpub_date
1988-11-25 00:00:00pages
99-104issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(88)90315-8journal_volume
115pub_type
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