Abstract:
:Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach that allows detection of specific antigen-presenting cells (APC) at a frequency of less than 0.0005%. When T cells are in excess, they form rosettes with rare APCs, which appear as single spots in an IFN-gamma ELISPOT assay. Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Dzutsev AK,Belyakov IM,Isakov DV,Gagnon SJ,Margulies DH,Berzofsky JAdoi
10.1016/j.jim.2008.01.008subject
Has Abstractpub_date
2008-04-20 00:00:00pages
71-8issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(08)00021-5journal_volume
333pub_type
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