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Production and characterization of a monoclonal antibody against Schistosoma japonicum glutathione S-transferase.

Abstract：

:Schistosoma japonicum glutathione S-transferase (GST), expressed from a pGEX plasmid, was isolated from Escherichia coli cells and used to immunize mice in order to generate specific anti-GST monoclonal antibodies. Using a modified immunization and fusion procedure, one stable hybridoma clone secreting an anti-GST antibody (alpha GST-1) was obtained. Milligram quantities of this antibody were produced in vitro in a miniPERM bioreactor and subsequently purified by protein G affinity chromatography. The characteristics of this antibody were investigated by enzyme-linked immunosorbent assays and immunoblotting experiments. The alpha GST-1 antibody was found to react specifically with GST and GST fusion proteins and demonstrated no reactivity with normal E. coli proteins. This monoclonal antibody should be a valuable reagent for tracing the production of GST fusion proteins and possibly for affinity purification of GST fusion proteins.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Campbell MJ,McFall P,Niederhuber JE

doi

10.1016/0022-1759(95)00204-9

subject

Has Abstract

pub_date

1995-12-15 00:00:00

pages

73-8

issue

1

eissn

0022-1759

issn

1872-7905

pii

0022175995002049

journal_volume

188

pub_type

杂志文章

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