Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro.

abstract：:The cell mediated immune response to herpes simplex virus was assessed by a modification of the leukocyte migration inhibition test, developed to increase its sensitivity and correlated with the in vitro stimulation of lymphocytes. The introduction of a preincubation step allowed us to demonstrate significant inhibiti...

journal_title：Journal of immunological methods

authors： Russell AS,Kaiser J,Lao VS

更新日期：1976-01-01 00:00:00

abstract：:We describe a raft technique for the study of chemotaxis which possesses a number of advantages over the use of chambers. Membranes are laid on pads soaked in chemotactic agent and cells are contained in plastic cups, which are inverted on to the membrane. This method gives results which are similar to those obtained ...

journal_title：Journal of immunological methods

authors： Addison IE,Babbage JW

更新日期：1976-01-01 00:00:00

abstract：:The length of the heavy chain complementarity-determining region two (HCDR2) of the unmutated anti-p-azophenylarsonate (Ars) monoclonal antibody (36-65 mAb) was extended by three residues in order to test whether this insertion can provide additional contacts between the Ab and the antigen. Two libraries were generate...

journal_title：Journal of immunological methods

authors： Parhami-Seren B,Viswanathan M,Margolies MN

更新日期：2002-01-01 00:00:00

abstract：:Use of fluorescently labelled multimers, particularly tetramers of peptide and MHC class I glycoprotein (pMHC-I) complexes, is essential for the analysis of CD8+ T cell immunity in basic research and clinical settings. A recently described combinatorial approach using pMHC-I multimers coupled to a unique combination o...

journal_title：Journal of immunological methods

authors： Cukalac T,Valkenburg SA,La Gruta NL,Turner SJ,Doherty PC,Kedzierska K

更新日期：2010-08-31 00:00:00

abstract：UNLABELLED:A defective ability of alveolar macrophages to phagocytose both apoptotic airway epithelial cells and bacteria in chronic lung diseases potentially associated with inflammation and bacterial colonisation of the lower airways, often with non-typeable Haemophilus influenzae (NTHi), has been shown. We routinely...

journal_title：Journal of immunological methods

authors： Ween M,Ahern J,Carroll A,Hodge G,Pizzutto S,Jersmann H,Reynolds P,Hodge S

更新日期：2016-02-01 00:00:00

abstract：:We describe a simplified and improved proliferation assay based on a conventional ELISA system for the in vitro measurement of cellular proliferation (ELISA proliferation assay = EPA). The assay is based on a new monoclonal antibody (Ki-S3) to the proliferation-specific Ki-67-antigen and is carried out in 96-well micr...

journal_title：Journal of immunological methods

authors： Frahm SO,Zott B,Dworeck C,Steinmann J,Neppert J,Parwaresch R

更新日期：1998-02-01 00:00:00

abstract：:Systems biology approaches that utilize large genomic data sets hold great potential for deciphering complex immunological process. In this paper, we propose such an approach to derive informative modules and networks from large gene expression data sets. Our approach starts with the clustering of such data sets to de...

journal_title：Journal of immunological methods

authors： Pandey G,Cohain A,Miller J,Merad M

更新日期：2013-01-31 00:00:00

abstract：:Using a solid-phase non-radioisotopic (non-RI) reverse transcriptase (RT) assay, antibodies inhibiting human immunodeficiency virus type 1 (HIV-1) RT activity (RTI antibody) were investigated for their ability to inhibit binding of RT to a template-primer and DNA polymerization. The RTI antibody inhibited the binding ...

journal_title：Journal of immunological methods

authors： Odawara F,Sano K,Otake T,Okubo S,Nakano T,Takasaki T,Ueba N,Misaki H,Nakai M

更新日期：1996-12-15 00:00:00

abstract：:An indirect immunofluorescence technique was developed for the detection of antibodies to dsDNA and the simultaneous assessment of antinuclear antibodies 'in toto' (ANA). This assay was based upon the use as substrate of smears of peripheral blood derived from rats infected with Trypanosoma lewisi. T. lewisi possesses...

journal_title：Journal of immunological methods

authors： Valesini G,Masala C

更新日期：1982-01-01 00:00:00

abstract：:The diagnostic and therapeutic applications of antibody single-chain Fv (sFv) fragments often require large amounts of protein that can be problematic and expensive to obtain. Here we report the secretion of two sFv fragments by the yeast Pichia pastoris at levels up to 250 mg/l. Soluble sFv fragments were purified fr...

journal_title：Journal of immunological methods

authors： Eldin P,Pauza ME,Hieda Y,Lin G,Murtaugh MP,Pentel PR,Pennell CA

更新日期：1997-02-14 00:00:00

abstract：:The structural diversity of the immune response may be considerably restricted by the structure of the hapten used to elicit catalytic antibodies. The ligand-binding mode and the shapes of the binding pockets of hydrolytic antibodies induced to different transition-state analogs that contain an unsubstituted arylphosp...

journal_title：Journal of immunological methods

authors： Golinelli-Pimpaneau B

更新日期：2002-11-01 00:00:00

abstract：:The concentrations of major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO) have been associated with eosinophilic disease severity. Whereas a variety of techniques have been used to measure individual eosinophil granule protein concentration,...

journal_title：Journal of immunological methods

authors： Makiya MA,Herrick JA,Khoury P,Prussin CP,Nutman TB,Klion AD

更新日期：2014-09-01 00:00:00

abstract：:Factors influencing the performance of a dipstick competitive particle capture immunoassay (PCI) for the steroid oestrone sulphate (OS) were investigated. Appropriate 'blocking' of the nitrocellulose dipstick membrane was necessary for the upward flow of microsphere particles. Traditional protein blocking agents inclu...

journal_title：Journal of immunological methods

authors： Henderson K,Stewart J

更新日期：2002-12-01 00:00:00

abstract：:Accurately predicting peptides binding to major histocompatibility complex (MHC) I molecules is of great importance to immunologists for elucidating the underlying mechanism of immune recognition and facilitating the design of peptide-based vaccine. Various computational methods have been developed for MHC I-peptide b...

journal_title：Journal of immunological methods

authors： Hu X,Mamitsuka H,Zhu S

更新日期：2011-11-30 00:00:00

abstract：:Recently, a powerful approach for the detection of MHC/peptide-specific T cells has been made possible by the engineering of soluble-tetrameric MHC/peptide complexes, consisting of singly biotinylated MHC/peptide molecules bound to fluorescent-labeled streptavidin. These tetrameric molecules are thought to compensate ...

journal_title：Journal of immunological methods

authors： Kalergis AM,Goyarts EC,Palmieri E,Honda S,Zhang W,Nathenson SG

更新日期：2000-02-03 00:00:00

abstract：:Antibodies to a wide range of self-antigens, including those directed against nucleic acids or nucleic acid-binding proteins are the essential biomarkers for diseases such as systemic lupus erythematosus (SLE). Highly complex libraries of nonamers consisting of N-substituted glycines (peptoids) were screened for compo...

journal_title：Journal of immunological methods

authors： Quan J,Lakhanpal A,Reddy MM,Zaman S,Li QZ,German DC,Olsen NJ,Kodadek T,Karp DR

更新日期：2014-01-15 00:00:00

abstract：:We describe the preparation of a new rat monoclonal antibody (CRC1) to the N-terminal sequence of the 43 kDa subunit of human ovarian inhibin, and its use together with other anti-peptide monoclonal antibodies, in two-site immunoassays for the detection of inhibin-related material in biological fluids. The Fab fractio...

journal_title：Journal of immunological methods

authors： Groome N,O'Brien M

更新日期：1993-10-15 00:00:00

abstract：:The IgG binding domains of staphylococcal protein A and streptococcal protein G were expressed as a chimaera using the pGEX vector which has been advocated because its fusion proteins tend to be soluble and easily isolated on immobilised glutathione. This chimaera was soluble and abundant (yield = 18 mg/l of bacterial...

journal_title：Journal of immunological methods

authors： Sun S,Lew AM

更新日期：1992-07-31 00:00:00

abstract：:Murine B16 melanoma cells and metastasis variants of this tumor are resistant to NK activity mediated by normal splenocytes. The B16 cells are, however, sensitive to splenocyte-mediated cytostasis. Cytostasis was measured by a [125I]UDR incorporation-inhibition [(125I]UDR I-I) assay. The main characteristics of the [1...

journal_title：Journal of immunological methods

authors： Ehrlich R,Efrati M,Witz IP

更新日期：1981-01-01 00:00:00

abstract：:A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein previously found to have a strong H-D a...

journal_title：Journal of immunological methods

authors： Mukuria JC,Naiki M,Hashimoto M,Nishiura K,Okabe M,Kato S

更新日期：1985-06-12 00:00:00

abstract：:There are several double immunolabelling methods but each has its drawbacks. More often than not, antibodies with the required specificities are available in only one species and their use normally produces false labels due to cross-reactivity. We describe a new and reliable technique for staining with primary antibod...

journal_title：Journal of immunological methods

authors： Eichmüller S,Stevenson PA,Paus R

更新日期：1996-04-19 00:00:00

abstract：:Purified ubiquitin has been shown to share similar biological and physicochemical properties with a previously characterized lymphokine, platelet activity suppressive lymphokine (PASL). This lymphokine, which inhibits the cytotoxic function of activated platelets, is produced during schistosomiasis and Hymenoptera ven...

journal_title：Journal of immunological methods

authors： Asseman C,Pancré V,Delanoye A,Capron A,Auriault C

更新日期：1994-07-12 00:00:00

abstract：:After centrifugation of heparinised whole blood on Hypaque-Ficoll medium (s.g. 1.114, Ferrante and Thong, 1980) the specific gravities were measured of the layers of medium above and below the band of polymorphonuclear leukocytes (PMN), as well as of the PMN and sedimented erythrocytes. The erythrocytes and PMN were a...

journal_title：Journal of immunological methods

authors： Bignold LP,Ferrante A

更新日期：1987-01-26 00:00:00

abstract：:A simple Terasaki tray-based ELISA technique with a fluorescent detecting system has been used to determine the affinity of murine IgE antibodies. The system was shown to be sensitive enough to measure affinities in the range of 10(-6)-10(-10) M as well as detect IgE antibodies down to a limit of 0.1 ng/ml. The result...

journal_title：Journal of immunological methods

authors： Pathak SS,Vos Q,Savelkoul HF

更新日期：1989-09-29 00:00:00

abstract：:IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is ...

journal_title：Journal of immunological methods

authors： Janot-Sardet C,Assouline B,Cheynier R,Morre M,Beq S

更新日期：2010-02-28 00:00:00

abstract：:In transplantation techniques previously described it was impossible to separate the effects of immunosuppressive treatments on the host from the effects directed at the allograft itself. The skin allograft retransplantation technique described here allows one to assess changes in the immunogenicity of the allograft g...

journal_title：Journal of immunological methods

authors： Sugarbaker PH,McCullough CS,Deutsch HL

更新日期：1984-09-04 00:00:00

abstract：:We describe here a specific and sensitive assay for biologically active bovine type-I interferon (IFN) in an Mx/CAT reporter gene assay. The assay is based on Madin-Darby Bovine Kidney cells transfected with a plasmid, containing a human MxA promoter driving a chloramphenicol acetyltransferase (CAT) cDNA. CAT expressi...

journal_title：Journal of immunological methods

authors： Fray MD,Mann GE,Charleston B

更新日期：2001-03-01 00:00:00

abstract：:Preclinical models of lymph node (LN) metastasis are fundamental to the study and design of new techniques for the diagnosis and treatment of LN metastasis. However, the identification of LNs and lymphatic vessels (LVs) in mice is challenging with conventional imaging modalities, since the LN diameter in normal mice i...

journal_title：Journal of immunological methods

authors： Shao L,Mori S,Yagishita Y,Okuno T,Hatakeyama Y,Sato T,Kodama T

更新日期：2013-03-29 00:00:00

abstract：:The influence of human hepatocyte growth factor (HGF) on the transforming growth factor beta (TGF-beta) bioassay CCL-64 was examined. HGF induced proliferation of the CCL-64 cells and potently counteracted TGF-beta-induced growth inhibition. HGF was not inactivated by transient acidification to pH 2, a commonly used p...

journal_title：Journal of immunological methods

authors： Börset M,Waage A,Sundan A

更新日期：1996-01-16 00:00:00

abstract：:Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of several therapeutic antibody drugs and evaluation in ADCC bioassays is important in antibody drug development and maintenance. Three types of effector cells now routinely used in bioassay evaluation of ADCC are natural ki...

journal_title：Journal of immunological methods

authors： Hsieh YT,Aggarwal P,Cirelli D,Gu L,Surowy T,Mozier NM

更新日期：2017-02-01 00:00:00