Quantitation of monoclonal immunoglobulins by immuno-isoelectric focusing and its application for monitoring secretory B cell neoplasia.

abstract：:Helicobacter pylori infection is the most common human infection where approximately 50% of the world populations are infected. The diagnosis of such infection is mainly done by endoscopy where gastric biopsies are examined for the presence of H. pylori. Such invasive approach is costly, time consuming and generally r...

journal_title：Journal of immunological methods

authors： Karakus C,Salih BA

更新日期：2013-10-31 00:00:00

abstract：:The performance of immunoassays for the detection of autoantibodies is of critical importance to the diagnosis and assessment of patients with systemic lupus erythematosus (SLE). Our objective was to compare 3 multiplexed assays for measurement of multiple autoantibodies and their association with global disease activ...

journal_title：Journal of immunological methods

authors： Hanly JG,Su L,Farewell V,Fritzler MJ

更新日期：2010-06-30 00:00:00

abstract：:We have studied the primate cellular requirements in the antibody forming cell (AFC) assay, using a 185,000 molecular mass streptococcal antigen. Cultures of B cells alone stimulated with the antigen were unable to develop into AFC. Reconstitution of B cells with T cells resulted in a significant increase in the numbe...

journal_title：Journal of immunological methods

authors： Fellowes R,Lehner T

更新日期：1990-09-14 00:00:00

abstract：:Establishment of humoral immunity against pathogens is dependent on events that occur in the germinal center and the subsequent induction of high-affinity neutralizing antibodies. Quantitative assays that allow monitoring of affinity maturation and duration of antibody responses can provide useful information regardin...

journal_title：Journal of immunological methods

authors： Lynch HE,Stewart SM,Kepler TB,Sempowski GD,Alam SM

更新日期：2014-02-01 00:00:00

abstract：:Human monoclonal antibodies may be generated by electrofusion of human B lymphocytes with a human/mouse heteromyeloma line. In addition to a fusion protocol optimised for the fusion partners, the activation of B lymphocytes is crucial for fusion and hybrid efficiency. In this study, we initially treated peripheral blo...

journal_title：Journal of immunological methods

authors： Schmidt E,Leinfelder U,Gessner P,Zillikens D,Bröcker EB,Zimmermann U

更新日期：2001-09-01 00:00:00

abstract：:Contamination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis samples has commonly produced artefactual bands when sensitive detection systems, such as silver staining or immunoblotting procedures, have been used. Evidence suggests that these bands originate from human skin contamination of reagents and ...

journal_title：Journal of immunological methods

authors： Riches PG,Polce B,Hong R

更新日期：1988-05-25 00:00:00

abstract：:A new, relatively simple, spectrophotometric technique has been developed which is useful for accurately determining the extent of chromophore labeling of proteins. Often the absorbance spectra and extinction coefficients of dye/protein conjugates are strongly affected by changes in the chromophore microenvironment th...

journal_title：Journal of immunological methods

authors： Meadows DL,Shafer JS,Schultz JS

更新日期：1991-10-25 00:00:00

abstract：:Bovine serum albumin (BSA) was used as a non-amphipathic model protein to be included into iscoms. Pretreatment at an acidic pH (2.5) was used to reveal hydrophobic regions, after which BSA could be integrated. In immunization experiments in mice the BSA iscoms induced long lasting and considerably higher serum antibo...

journal_title：Journal of immunological methods

authors： Morein B,Ekström J,Lövgren K

更新日期：1990-04-17 00:00:00

abstract：:The polymerase chain reaction (PCR) has proved to be a sensitive and versatile method for the analysis of human and murine cytokine mRNA expression. This paper describes for the first time a reverse transcription-polymerase chain reaction (RT-PCR) at end-point for the quantification of five porcine cytokines: interfer...

journal_title：Journal of immunological methods

authors： Dufour V,Arnauld C,Lantz O,Peguillet I,Morvilliers K,Emmanuel A,Jestin A

更新日期：1999-10-29 00:00:00

abstract：:A simplified technique for the production of chimeric thymus completely in vitro from the third pharyngeal pouch (TPP) of 10-day mouse embryos has been examined. The results are similar to those obtained by a more cumbersome technique published by others. This modified technique permits the production of large numbers...

journal_title：Journal of immunological methods

authors： Pyke KW,Bartlett PF,Mandel TE

更新日期：1983-03-11 00:00:00

abstract：:Information about the IgE receptor and IgE content of mast cells under different conditions in vivo is essential for further understanding the functions of the mast cell-IgE system. A cytofluorometric method for measuring cell-bound IgE on individual mast cells was therefore explored using peritoneal mast cells of Spr...

journal_title：Journal of immunological methods

authors： Chen XJ,Juliusson S,Aldenborg F,Enerbäck L

更新日期：1994-12-28 00:00:00

abstract：:alpha-2-macroglobulin (alpha-2-M), a plasma proteinase inhibitor, plays an important role in the pathogenesis of lung inflammation. The purpose of this study was to develop a sensitive ELISA assay for rabbit alpha-2-M to allow us to define the role of this protein in a rabbit model of lung inflammation/injury. Therefo...

journal_title：Journal of immunological methods

authors： Kurdowska A,Miller EJ,Krupa A,Noble JM,Sakao Y

更新日期：2002-12-15 00:00:00

abstract：:Human tumor infiltrating lymphocytes (TIL) from metastatic melanoma of six patients were grown using a new hollow fiber bioreactor system. After inoculating 0.35-10 X 10(8) TIL into the extra-fiber space (EFS), each Cellmax bioreactor was perfused with AIM-V medium, supplemented with rIL-2. The cells subsequently expa...

journal_title：Journal of immunological methods

authors： Knazek RA,Wu YW,Aebersold PM,Rosenberg SA

更新日期：1990-02-20 00:00:00

abstract：:A sandwich amperometric enzyme immunoassay with flow injection for alpha-fetoprotein in human serum has been developed with alkaline phosphatase as the enzyme label. p-Hydroxyphenyl phosphate was used as the substrate for alkaline phosphatase. The hydrolysis product, hydroquinone, was detected by oxidative amperometry...

journal_title：Journal of immunological methods

authors： Della Ciana L,Bernacca G,De Nitti C,Massaglia A

更新日期：1996-06-14 00:00:00

abstract：:We report a procedure for optimisation of Western blotting using protein G-horseradish peroxidase (protein G-HRP) which avoids the false positive reactions often caused by second antibodies and increases the detection of autoantibodies by protein G conjugate. A number of modifications were investigated. Higher concent...

journal_title：Journal of immunological methods

authors： Hu GR,Harrop P,Warlow RS,Gacis ML,Walls RS

更新日期：1997-03-28 00:00:00

abstract：:Somatic cell hybridization between the YACUT T cell lymphoma cell line with normal lymphocytes activated in secondary mixed lymphocyte cultures (MLCs) consistently yielded IL-2-dependent CD4- CD8 alpha+ beta- Fc gamma RIII+ hybrids with cytotoxic function. The hybrids expressed T cell receptors other than that of YACU...

journal_title：Journal of immunological methods

authors： Kubota K,Nakazato K,Tamauchi H,Sasahara T,Katoh H

更新日期：1996-06-10 00:00:00

abstract：:Development of cell lines for biotherapeutic protein production requires screening large numbers of clones to identify and isolate high producing ones. As such, stable cell line generation is a time- and resource-intensive process. There is an increasing need to enhance the selection efficiency of high-yielding clonal...

journal_title：Journal of immunological methods

authors： Chakrabarti L,Mathew A,Li L,Han S,Klover J,Albanetti T,Hawley-Nelson P

更新日期：2019-01-01 00:00:00

abstract：:In the present study we describe a technique for the preparation of a highly purified B cell population from peripheral blood mononuclear cells (PBMC) by immune rosette depletion of non-B cells utilizing monoclonal antibodies directed at T cells, monocytes and null cells. The non-rosetted population contains greater t...

journal_title：Journal of immunological methods

authors： Anderson KC,Griffin JD,Bates MP,Slaughenhoupt BL,Schlossman SF,Nadler LM

更新日期：1983-07-29 00:00:00

abstract：:A proliferation assay to detect a specific T cell response against HLA class I-binding peptides is reported. To establish the specificity and sensitivity of the assay we used a synthetic peptide derived from the CMV glycoprotein B containing the HLA-A2.1 ligand motif. Lymphocyte proliferation was measured following cu...

journal_title：Journal of immunological methods

authors： Mayer S,Scheibenbogen C,Lee KH,Keilholz W,Stevanovic S,Rammensee HG,Keilholz U

更新日期：1996-10-16 00:00:00

abstract：:A unique method for assessing the in vivo recovery of cellular immunity following sublethal irradiation and syngeneic bone marrow transplantation of CBA/J mice is described. This method employs the serial surgical implantation of a urethane sponge matrix followed by impregnation of the sponge matrix with alloantigen. ...

journal_title：Journal of immunological methods

authors： Jenski LJ,Carter DL,Denman DL,Lampkin BC

更新日期：1985-12-17 00:00:00

abstract：:In this study we describe a fast, simple, and objective flow cytometry method to quantify simultaneously phagocytosis and Fc gamma R-mediated oxidative burst in human monocytes. Peripheral blood monocytes isolated by elutriation were sequentially incubated with 2',7'-dichlorofluorescein-diacetate (DCFH-DA) and sheep e...

journal_title：Journal of immunological methods

authors： Casado JA,Merino J,Cid J,Subira ML,Sanchez-Ibarrola A

更新日期：1993-02-26 00:00:00

abstract：:Monoclonal antibodies were used to demonstrate hybrid forms of human alkaline phosphatase (ALP) composed of subunits from both placental and intestinal loci. Four main isozymes with alkaline phosphatase activity appear on polyacrylamide gels of the HeLa cell line, Hep 2/5 after electrophoresis. The mobilities of all 4...

journal_title：Journal of immunological methods

authors： Wray L,Harris H

更新日期：1982-11-26 00:00:00

abstract：:An assay to detect circulating immune complexes is described and exemplified with serum from SLE and RA patients. The method is based on selective precipitation of immune complexes with PEG followed by a specific ELISA assay to detect the immunoglobulins present in the precipitate. The immunoglobulins of the precipita...

journal_title：Journal of immunological methods

authors： Ohlson S,Zetterstrand K

更新日期：1985-02-28 00:00:00

abstract：:The immune system is characterized by high combinatorial complexity that necessitates the use of specialized computational tools for analysis of immunological data. Machine learning (ML) algorithms are used in combination with classical experimentation for the selection of vaccine targets and in computational simulati...

journal_title：Journal of immunological methods

authors： Zhang GL,Lin HH,Keskin DB,Reinherz EL,Brusic V

更新日期：2011-11-30 00:00:00

abstract：:A method of tanned erythrocyte agglutination of wide applicability is described for the study of DNA antibodies. The practical aspects of the method have been explored in estimation of antibodies to DNA in human cases of systemic lupus erythematosus, discoid lupus erythematosus, rheumatoid arthritis, other collagenose...

journal_title：Journal of immunological methods

authors： Depiante-Depaoli M,Riera CM,Yantorno C,Risemberg A,Strussberg A

更新日期：1978-01-01 00:00:00

abstract：:The multiple antigen peptide (MAP) system has been proposed as a novel and valuable approach for eliciting antibodies to peptides and developing synthetic vaccines. The MAP system consists of a small immunogenically inert core matrix of lysine residues with alpha- and epsilon-amino groups for anchoring multiple copies...

journal_title：Journal of immunological methods

authors： Briand JP,Barin C,Van Regenmortel MH,Muller S

更新日期：1992-12-08 00:00:00

abstract：:The optimal conditions for the detection of outer membrane proteins (OMPs) and lipopolysaccharide (LPS) of Pasteurella haemolytica by immunoblotting were evaluated. The variables examined included the equilibration time of the gels before transfer, composition of the transfer buffer, type of blotting membrane, blockin...

journal_title：Journal of immunological methods

authors： Davies RL,Parton R,Coote JG,Gibbs HA,Freer JH

更新日期：1994-01-03 00:00:00

abstract：:The method of extraction of outer membrane proteins (OMPs), the conditions of electrophoretic transfer, and the conditions of antibody binding, were compared in Western blotting studies of Neisseria meningitidis outer membrane antigens. The OMP profiles obtained by SDS-PAGE of outer membrane vesicles extracted with li...

journal_title：Journal of immunological methods

authors： Davies RL,Wall RA,Borriello SP

更新日期：1990-12-05 00:00:00

abstract：:Limiting dilution (LD) cultures are often used to study cellular heterogeneity in responses of murine splenocytes to specific or polyclonal activation. LD titration curves often reveal a nonlinear dependence of response on input cell dose. Although 'zigzag' shaped curves of this kind are often interpreted and analyzed...

journal_title：Journal of immunological methods

authors： Dozmorov IM,Lutsenko GV,Sidorov LA,Miller RA

更新日期：1996-02-05 00:00:00

abstract：:A simple roller bottle was constructed to house three dialysis tubes, each with a capacity of 75 ml. Cells were grown inside the dialysis tubing, which was immersed in ordinary DMEM medium without serum supplement. Cultures of hybridomas at medium or low density (2 X 10(5) cells/ml) could be expanded directly in the d...

journal_title：Journal of immunological methods

authors： Pannell R,Milstein C

更新日期：1992-01-21 00:00:00