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An investigation of the T cell requirements of in vitro antibody forming B cells detected by the ELISPOT assay and a comparison with antibody synthesis.

Abstract：

:We have studied the primate cellular requirements in the antibody forming cell (AFC) assay, using a 185,000 molecular mass streptococcal antigen. Cultures of B cells alone stimulated with the antigen were unable to develop into AFC. Reconstitution of B cells with T cells resulted in a significant increase in the number of AFC. However, CD4 cells were more efficient than T cells in helping the antibody response and CD8 cells failed to induce B cells to synthesise antibody. A comparison between the specific IgG antibody synthesis and the number of AFC detected by the ELISPOT method showed a highly significant correlation between the number of AFC and the amount of specific IgG antibody. We suggest that the AFC (ELISPOT) assay can be readily used to investigate B cell interactions with T cell subsets.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Fellowes R,Lehner T

doi

10.1016/0022-1759(90)90026-r

subject

Has Abstract

pub_date

1990-09-14 00:00:00

pages

165-72

issue

2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(90)90026-R

journal_volume

132

pub_type

杂志文章

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