Abstract：
:The SELEX (Systematic Evolution of Ligands by EXponential enrichment) method has been used successfully since 1990, but work is still required to obtain highly specific aptamers. Here, we present a novel approach called 'Competitive non-SELEX' (and termed as 'SELCOS' (Systematic Evolution of Ligands by COmpetitive Selection)) for readily obtaining aptamers that can discriminate between highly similar targets. This approach is based on the theoretical background presented here, in which under the co-presence of two similar targets, a specific binding type can be enriched more than a nonspecifically binding one during repetitive steps of partitioning with no PCR amplification between them. This principle was experimentally confirmed by the selection experiment for influenza virus subtype-specific DNA aptamers. Namely, the selection products (pools of DNA aptamers) obtained by SELCOS were subjected to a DEPSOR-mode electrochemical sensor, enabling the method to select subtype-specific aptamer pools. From the clonal analysis of these pools, only a few rounds of in vitro selection were sufficient to achieve the surprisingly rapid enrichment of a small number of aptamers with high selectivity, which could be attributed to the SELCOS principle and the given selection pressure program. The subtype-specific aptamers obtained in this manner had a high affinity (e.g., KD = 82 pM for H1N1; 88 pM for H3N2) and negligible cross-reactivity. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kushwaha A,Takamura Y,Nishigaki K,Biyani Mdoi
10.1038/s41598-019-43187-6subject
Has Abstractpub_date
2019-04-30 00:00:00pages
6642issue
1issn
2045-2322pii
10.1038/s41598-019-43187-6journal_volume
9pub_type
杂志文章abstract：:A possible association between depression and either the severity of constipation or dysosmia in Parkinson's disease (PD) patients was investigated in this cross-sectional study. One-hundred six patients who had the history of PD for less than 5 years were recruited. Depression was measured using the Beck Depression I...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72381-0
更新日期：2020-09-23 00:00:00
abstract：:The novel nuclear protein nBMP2 is synthesized from the BMP2 gene by translational initiation at an alternative start codon. We generated a targeted mutant mouse, nBmp2NLStm, in which the nuclear localization signal (NLS) was inactivated to prevent nuclear translocation of nBMP2 while still allowing the normal synthes...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46464
更新日期：2017-04-18 00:00:00
abstract：:Genome-wide studies on high-altitude adaptation have received increased attention as a classical case of organismal evolution under extreme environment. However, the current genetic understanding of high-altitude adaptation emanated mainly from autosomal analyses. Only a few earlier genomic studies paid attention to t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35004
更新日期：2016-10-07 00:00:00
abstract：:Neuropsychological assessment of human visual processing capabilities strongly depends on visual testing conditions including room lighting, stimuli, and viewing-distance. This limits standardization, threatens reliability, and prevents the assessment of core visual functions such as visual processing speed. Increasin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37016
更新日期：2016-11-21 00:00:00
abstract：:Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25030
更新日期：2016-04-29 00:00:00
abstract：:p53 and aldehyde dehydrogenase (ALDH) have been implicated in key tumorigenesis processes including cancer initiating cell (CIC) maintenance; however, the relationship between these two mediators remains poorly defined. In this study, ALDH isoform expression diversity was revealed in CICs with disparate p53 functional...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57758-5
更新日期：2020-01-23 00:00:00
abstract：:Clinically isolated syndrome (CIS) is the earliest clinical episode in multiple sclerosis (MS). Low environmental exposure to UV radiation is implicated in risk of developing MS, and therefore, narrowband UVB phototherapy might delay progression to MS in people with CIS. Twenty individuals with CIS were recruited, and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44488-6
更新日期：2019-05-28 00:00:00
abstract：:The rapid pace of conversion of natural areas to agricultural systems is highly concerning, and the consequences for conservation and pest management are not yet fully understood. We examined mitochondrial (COI and Cytb) and nuclear (ITS1) gene regions of 21 populations of the stink bug Euschistus heros, to investigat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20187-6
更新日期：2018-02-06 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-57947-2
更新日期：2020-01-30 00:00:00
abstract：:The present study evaluates the potential of Sar gassum muticum (Sar) and Jan ia rubens (Jan) seaweeds for enhancing growth and mitigating soil-salinity in chickpea (Cicer arietinum L.). Under control conditions, Sar and Jan extracts improved chickpea growth which was attributed to their potential for increasing photo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07692-w
更新日期：2017-09-05 00:00:00
abstract：:After hepatic microwave ablation, the differentiation between fully necrotic and persistent vital tissue through contrast enhanced CT remains a clinical challenge. Therefore, there is a need to evaluate new imaging modalities, such as CT perfusion (CTP) to improve the visualization of coagulation necrosis. MWA and CTP...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55026-9
更新日期：2019-12-06 00:00:00
abstract：:It is essential to understand how the loss of biodiversity impacts both ecosystem function (EF) and multifunctionality (EMF). Previous studies have mostly focused on predicting how species richness (SR) impacts EMF, while the effect of functional diversity (FD) on EMF remains unclear. Specifically, we know little abou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43475-1
更新日期：2019-05-06 00:00:00
abstract：:The ability of human metaphase-II arrested eggs to activate following fertilisation declines with advancing maternal age. Egg activation is triggered by repetitive increases in intracellular Ca(2+) concentration ([Ca(2+)]i) in the ooplasm as a result of sperm-egg fusion. We therefore hypothesised that eggs from older ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19357
更新日期：2016-01-20 00:00:00
abstract：:We report efficient detection of alpha radiation on highly textured and vertically aligned along (002) Gallium-doped Zinc Oxide (ZnO:Ga) nanorods on a glass substrate with an average diameter ~150 ± 10 nm. Photoluminescence measurement showed near band emission 393 nm, in agreement with the bandgap value ~3.22 eV, mea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47732-1
更新日期：2019-08-06 00:00:00
abstract：:To survive and replicate in macrophages Mycobacterium tuberculosis (Mtb) has developed strategies to subvert host defence mechanisms, including autophagy. Autophagy induction has the potential to clear Mtb, but little is known about its effect during controlled tuberculosis and HIV co-infection. Mammalian target of ra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28171
更新日期：2016-06-15 00:00:00
abstract：:Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81789-1
更新日期：2021-01-28 00:00:00
abstract：:A single closed-form analytical solution of the driven nonlinear Schrödinger equation is developed, reproducing a large class of the behaviors in Kerr-comb systems, including bright-solitons, dark-solitons, and a large class of periodic wavetrains. From this analytical framework, a Kerr-comb area theorem and a pump-de...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24742
更新日期：2016-04-25 00:00:00
abstract：:Measurements of volcanic tephra fallout deposits provide useful information about the magnitude and intensity of explosive volcanic eruptions and potential for remobilization of deposits as dangerous volcanic flows. However, gathering information in the vicinity of erupting craters is extremely dangerous, and moreover...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71902-1
更新日期：2020-09-09 00:00:00
abstract：:The purpose of this meta-analysis was to compare the efficacy and safety of regional anesthesia to manage chronic postsurgery pain. A systematic search of PubMed, EmBase, and the Cochrane Central Register of Controlled Trials was performed to identify randomized controlled trials that focused on chronic pain frequency...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-017-00813-5
更新日期：2017-04-06 00:00:00
abstract：:During dental erosion, tooth minerals are dissolved, leading to a softening of the surface and consequently to irreversible surface loss. Components from human saliva form a pellicle on the tooth surface, providing some protection against erosion. To assess the effect of different components and compositions of saliva...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34760
更新日期：2016-10-05 00:00:00
abstract：:Ebola virus disease (EVD) is a contagious, severe and often lethal form of hemorrhagic fever in humans. The association of EVD outbreaks with forest clearance has been suggested previously but many aspects remained uncharacterized. We used remote sensing techniques to investigate the association between deforestation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14727-9
更新日期：2017-10-30 00:00:00
abstract：:Survival of many species, from insects and birds to human and non-human mammals, requires synchronized activity. Among humans, synchrony occurs even at the level of autonomic functioning; people interacting often show mutual, simultaneous changes in activity of the sympathetic or parasympathetic branches of the autono...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44667-5
更新日期：2019-06-03 00:00:00
abstract：:To attach reliably on various inclined rough surfaces, many insects have evolved both claws and adhesive pads on their feet. However, the interaction between these organs still remains unclear. Here we designed an artificial attachment device, which mimics the structure and function of claws and adhesive pads, and tes...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26219
更新日期：2016-05-20 00:00:00
abstract：:Hypertension is the most common and lethal risk factor for cardiovascular disease (CVD). Numerous variants have been associated with hypertension, however, most of which failed to get replication due to ethnic differences. In this study, we analyzed associations of 10 newly reported single nucleotide polymorphisms (SN...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15245
更新日期：2015-10-21 00:00:00
abstract：:High-precision radiotherapy (HPR) has established its important role in the treatment of tumors due to its precise dose distribution. Given its more complicated delivery process, HPR commonly requires more fraction delivery time (FDT). However, it is unknown whether it has an identical response of prolonged FDT on dif...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09119
更新日期：2015-03-13 00:00:00
abstract：:Familial breast cancer is estimated to account for 15-20% of all cases of breast cancer. Surveillance for familial breast cancer is well-established world-wide. However, this service does not exist in Jordan, due to the scarcity of information with regard to the genetic profiling of these patients, and therefore lack ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74250-2
更新日期：2020-10-16 00:00:00
abstract：:Prior ex vivo histological postmortem studies of autism spectrum disorder (ASD) have shown gray matter microstructural abnormalities, however, in vivo examination of gray matter microstructure in ASD has remained scarce due to the relative lack of non-invasive methods to assess it. The aim of this work was to evaluate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78486-w
更新日期：2020-12-08 00:00:00
abstract：:Pain is a harmful sensation that arises from noxious stimuli. Transient receptor potential ankyrin 1 (TRPA1) is one target for studying pain mechanisms. TRPA1 is activated by various stimuli such as noxious cold, pungent natural products and environmental irritants. Since TRPA1 is an attractive target for pain therapy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37460
更新日期：2016-11-22 00:00:00
abstract：:The quest to 'forward-engineer' and fabricate biological machines remains a grand challenge. Towards this end, we have fabricated locomotive "bio-bots" from hydrogels and cardiomyocytes using a 3D printer. The multi-material bio-bot consisted of a 'biological bimorph' cantilever structure as the actuator to power the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00857
更新日期：2012-01-01 00:00:00
abstract：:The cell-to-module efficiency gap in Cu(In,Ga)Se2 (CIGS) monolithically integrated solar modules is enhanced by contact resistance between the Al-doped ZnO (AZO) and Mo back contact layers, the P2 contact, which connects adjacent cells. The present work evaluated the P2 contact resistance, in addition to the TCO resis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07690
更新日期：2015-01-09 00:00:00
