Abstract：
:Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complete replacement of eukaryotic chromosomes with heterologous DNA. The moss Physcomitrella patens, a non-vascular and spore producing land plant (Bryophyte), has a well-established capacity for homologous recombination. Here, we demonstrate the in vivo assembly of multiple DNA fragments in P. patens with three examples of effective genome editing: we (i) efficiently deleted a genomic locus for diterpenoid metabolism yielding a biosynthetic knockout, (ii) introduced a salt inducible promoter, and (iii) re-routed endogenous metabolism into the formation of amorphadiene, a precursor of high-value therapeutics. These proof-of-principle experiments pave the way for more complex and increasingly flexible approaches for large-scale metabolic engineering in plant biotechnology.
journal_name
Sci Repjournal_title
Scientific reportsauthors
King BC,Vavitsas K,Ikram NK,Schrøder J,Scharff LB,Bassard JÉ,Hamberger B,Jensen PE,Simonsen HTdoi
10.1038/srep25030subject
Has Abstractpub_date
2016-04-29 00:00:00pages
25030issn
2045-2322pii
srep25030journal_volume
6pub_type
杂志文章abstract：:Applications of ferroelectric materials in modern microelectronics will be greatly encouraged if the thermal incompatibility between inorganic ferroelectrics and semiconductor devices is overcome. Here, solution-processable layers of the most commercial ferroelectric compound--morphotrophic phase boundary lead zircona...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20143
更新日期：2016-02-03 00:00:00
abstract：:Novel mesoporous silicas rolled with silica sheets with 2D regular spacing were prepared using a self-assembled graphene oxide (GO) template formed by mixing GO with Pluronic123 (P123). Self-assembled GO templated mesoporous silicas (SGT-PMS) showed well-developed X-ray diffraction peaks with d-spacings of 9.8-10.8 nm...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63017-4
更新日期：2020-04-10 00:00:00
abstract：:The psychrophilic (cold-loving) fungus Pseudogymnoascus destructans was discovered more than a decade ago to be the pathogen responsible for white-nose syndrome, an emerging disease of North American bats causing unprecedented population declines. The same species of fungus is found in Europe but without associated mo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70375-6
更新日期：2020-08-17 00:00:00
abstract：:Suffering from giant size of objective lenses and infeasible manipulations of distant targets, telescopes could not seek helps from present super-resolution imaging, such as scanning near-field optical microscopy, perfect lens and stimulated emission depletion microscopy. In this paper, local light diffraction shrinka...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18485
更新日期：2015-12-18 00:00:00
abstract：:Cancer vaccines are less effective at old than at young age because of immunosenescence. Besides, in preliminary observations we showed that the immunization with HER-2/neu DNA plasmid in transgenic young mice (standard immunization, SI) delays but not abrogate spontaneous mammary tumours progressively appearing durin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03286-8
更新日期：2017-06-08 00:00:00
abstract：:Polyphenol oxidases (PPOs) comprise tyrosinases (TYRs) and catechol oxidases (COs), which catalyse the initial reactions in the biosynthesis of melanin. TYRs hydroxylate monophenolic (monophenolase activity) and oxidize diphenolic (diphenolase activity) substrates, whereas COs react only with diphenols. In order to el...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57671-x
更新日期：2020-02-03 00:00:00
abstract：:Power induced wave collapse is one of the most fascinating phenomena in optics as it provides extremely high intensities, thus stimulating a range of nonlinear processes. For low power levels, propagation of beams in bulk media is dominated by diffraction, while above a certain threshold self-focusing is steadily enha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46060-8
更新日期：2019-07-02 00:00:00
abstract：:Oligomerization of protein into specific quaternary structures plays important biological functions, including regulation of gene expression, enzymes activity, and cell-cell interactions. Here, we report the determination of two crystal structures of the Grimontia hollisae (formally described as Vibrio hollisae) therm...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46354-x
更新日期：2019-07-08 00:00:00
abstract：:Activation of G protein-gated inwardly rectifying potassium (GIRK) channels leads to a hyperpolarization of the neuron's membrane potential, providing an important component of inhibition in the brain. In addition to the canonical G protein-activation pathway, GIRK channels are activated by small molecules but less is...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04681-x
更新日期：2017-07-04 00:00:00
abstract：:Crystalline atomic layers on solid surfaces are composed of a single building block, unit cell, that is copied and stacked together to form the entire two-dimensional crystal structure. However, it appears that this is not an unique possibility. We report here on synthesis and characterization of the one-atomic-layer-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19446
更新日期：2016-01-19 00:00:00
abstract：:Plasma free amino acid (PFAA) profile is highlighted in its association with visceral obesity and hyperinsulinemia, and future diabetes. Indeed PFAA profiling potentially can evaluate individuals' future risks of developing lifestyle-related diseases, in addition to diabetes. However, few studies have been performed e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11918
更新日期：2015-07-09 00:00:00
abstract：:We aimed to investigate the changes of olfaction of major depressive disorder (MDD) before and after medical treatment, and to preliminarily scrutinize the association between the olfactory function and the severity of depressive symptoms, response inhibition, and emotional responding. Forty-eight medicine-naïve MDD p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63416-7
更新日期：2020-04-14 00:00:00
abstract：:One of the most exciting areas in lithium ion batteries is engineering structured silicon anodes. These new materials promise to lead the next generation of batteries with significantly higher reversible charge capacity than current technologies. One drawback of these materials is that their production involves costly...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00795
更新日期：2012-01-01 00:00:00
abstract：:Electrochemically generated bactericidal compounds have been shown to eradicate bacterial lawn biofilms through electroceutical treatment. However, the ultrastructure of biofilms exposed to these species has not been studied. Moreover, it is unknown if the efficacy of electroceutical treatment extends to antibiotic-re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66823-y
更新日期：2020-06-18 00:00:00
abstract：:Currently, a comprehensive assessment between mitochondrial DNA (mtDNA) content and cancer risk is lacking. We designed this meta-analysis to test the hypothesis that altered mtDNA copy number might influence genetic susceptibility to some specific types of cancer. The processes of literature search, eligibility appra...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep10039
更新日期：2015-05-08 00:00:00
abstract：:The exploitation of surface plasmon polaritons has been mostly limited to the visible and near infrared range, due to the low frequency limit for coherent plasmon excitation and the reduction of confinement on the metal surface for lower energies. In this work we show that 3D--out of plane--nanostructures can consider...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16436
更新日期：2015-11-10 00:00:00
abstract：:The impacts of ecosystem engineers may be expected to vary along environmental gradients. Due to some resources being more limited in arid than in mesic environments, disturbances created by burrowing mammals are expected to have a greater ameliorating effect in arid environments, with larger differences in microhabit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55917-x
更新日期：2019-12-18 00:00:00
abstract：:Hard carbon attracts wide attentions as the anode for high-energy rechargeable batteries due to its low cost and high theoretical capacities. However, the intrinsically disordered microstructure gives it poor electrical conductivity and unsatisfactory rate performance. Here we report a facile synthesis of N-doped grap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28310-3
更新日期：2018-07-02 00:00:00
abstract：:A detailed molecular understanding of botulinum neurotoxin (BoNT)/host-cell-receptor interactions is fundamental both for developing strategies against botulism and for generating improved BoNT variants for medical applications. The X-ray crystal structure of the receptor-binding domain (HC) of BoNT/A1 in complex with...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43588
更新日期：2017-03-02 00:00:00
abstract：:The salmon louse is an ectoparasitic copepod of salmonids in the marine environment, and represents a global challenge to salmon aquaculture. A major issue is the reliance of the industry on a limited number of chemicals to delouse salmonids on farms, and the high levels of resistance that lice have developed to all o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12384-6
更新日期：2017-09-27 00:00:00
abstract：:Gluconobacter oxydans sorbitol dehydrogenase (GoSLDH) exhibits a higher catalytic efficiency than other L-sorbose producing enzymes. During the reaction catalysed by GoSLDH, NADP+ is reduced to NADPH and D-sorbitol is oxidized to L-sorbose. However, GoSLDH activity is inhibited by the NADPH (Ki = 100 μM) formed during...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37401-0
更新日期：2019-01-28 00:00:00
abstract：:Parkinson's Disease can be understood as a disorder of motor habits. A prediction of this theory is that early stage Parkinson's patients will display fewer errors caused by interference from previously over-learned behaviours. We test this prediction in the domain of skilled typing, where actions are easy to record a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39294-z
更新日期：2019-03-04 00:00:00
abstract：:This study utilized a Förster resonance energy transfer (FRET)-based molecular tension sensor and live cell imaging to evaluate the effect of osteocytes, a mechanosensitive bone cell, on the migratory behavior of tumor cells. Two cell lines derived from MDA-MB-231 breast cancer cells were transfected with the vinculin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42132-x
更新日期：2019-04-04 00:00:00
abstract：:To identify novel phenotypic associations related to Cytochrome P450 Family 2 Subfamily A Member 6 (CYP2A6), we investigated the human phenome in a total of 11,271 individuals. Initially, we conducted a phenome-wide association study in 3,401 nicotine-exposed elderly subjects considering 358 phenotypic traits. We iden...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01098-4
更新日期：2017-04-21 00:00:00
abstract：:Herein, we have fabricated rutile TiO2 nanorod-coupled α-Fe2O3 by a wet-chemical process. It is demonstrated that the visible activities for photoelectrochemical water oxidation and for degrading pollutant of α-Fe2O3 are greatly enhanced after coupling a proper amount of rutile nanorods. The enhanced activity is attri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06180
更新日期：2014-08-26 00:00:00
abstract：:Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFRα+Flk1- cardioblasts (PCBs), from mouse and human plurip...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41840
更新日期：2017-02-06 00:00:00
abstract：:The aim of this study was to assess the association between the shoulder tenderness and the inflammatory changes on magnetic resonance imaging (MRI) in the rheumatoid shoulder. Forty-one shoulders of 41 patients with rheumatoid arthritis (RA) were examined. We evaluated synovitis, erosion and bone marrow edema, by cou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55938-6
更新日期：2019-12-20 00:00:00
abstract：:The aim of the present study was to evaluate the association between statin use and the risk of age-related macular degeneration (AMD). A systematic search of the PubMed, EMBASE and ISI web of science databases was used to identify eligible published literatures without language restrictions up to April 2015. Summary ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep18280
更新日期：2015-12-14 00:00:00
abstract：:An accurate prediction of the outcome of a given drug treatment requires quantitative values for all parameters and concentrations involved as well as a detailed characterization of the network of interactions where the target molecule is embedded. Here, we present a high-throughput in silico screening of all potentia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30913-9
更新日期：2018-08-21 00:00:00
abstract：:Contest behaviour, and in particular the propensity to attack an unfamiliar conspecific, is influenced by an individual's aggressiveness, as well as by experience of winning and losing (so called 'winner-loser effects'). Individuals vary in aggressiveness and susceptibility to winner-loser effects but the relationship...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69664-x
更新日期：2020-08-07 00:00:00
