Abstract：
Background:Endoplasmic reticulum stress (ERS) in the retinal Müller cells is a key factor contributing to the retinal inflammation and vascular leakage in diabetic retinopathy (DR). This study was to investigate the underlying mechanisms through which the 3 main unfolded protein response (UPR) pathways regulate ERS and to examine the expression levels of vascular endothelial growth factor (VEGF) in Müller cells in vitro. Methods:Rat Müller cell lines were stimulated with high glucose to mimic a diabetic environment in vitro. PKR-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme 1 (IRE1) and activating transcription factor 6 (ATF6) were downregulated or upregulated with shRNA or overexpression plasmids. The transfected Müller cells were cultivated in high glucose medium for 48 hours. Expression of glucose-regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), X-box binding protein 1 (XBP1), ATF6, and VEGF was examined with immunofluorescence and western blot. Results:Our data indicated that ERS was found in both high glucose and osmotic control groups. Overexpression or downregulation of UPR pathways effectively increased or reduced the production of GRP78, ATF4, XBP1, ATF6, and VEGF, respectively. These 3 signaling pathways had similar regulatory effects on VEGF. Conclusion:The 3 UPR-mediated inflammatory pathways were dependent on each other. Inhibition any of these signaling pathways in UPR might be a potential therapeutic target for DR.
journal_name
J Ophthalmoljournal_title
Journal of ophthalmologyauthors
Wu S,Zhu X,Guo B,Zheng T,Ren J,Zeng W,Chen X,Ke Mdoi
10.1155/2019/9028483subject
Has Abstractpub_date
2019-02-24 00:00:00pages
9028483eissn
2090-004Xissn
2090-0058journal_volume
2019pub_type
杂志文章abstract：:Idiopathic full-thickness macular hole is a severe visual impairment disease. Pars plana vitrectomy remains the primary treatment option for idiopathic full-thickness macular holes, and over 90% idiopathic full-thickness macular holes are closed by vitrectomy surgery. However, the full-thickness macular hole may close...
journal_title：Journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1155/2019/4793764
更新日期：2019-03-13 00:00:00
abstract：:Purpose. To examine the influence of positional misalignments on intraocular pressure (IOP) measurement with a rebound tonometer. Methods. Using the iCare rebound tonometer, IOP readings were taken from the right eye of 36 healthy subjects at the central corneal apex (CC) and compared to IOP measures using the Goldman...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2013/791084
更新日期：2013-01-01 00:00:00
abstract：:Purpose. To investigate the local relationship between quantified global-flash multifocal electroretinogram (mfERG) optic nerve head component (ONHC) and visual field defects in patients with glaucoma. Methods. Thirty-nine patients with glaucoma and 30 normal controls were enrolled. The ONHC amplitude was measured fro...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2015/397495
更新日期：2015-01-01 00:00:00
abstract：PURPOSE:To evaluate the static measurement of the accommodative stimulus-response curve with emoji symbols. METHODS:The accommodative stimulus-response curve was measured in 18 subjects using a Hartmann-Shack sensor to obtain the objective accommodative response from the Zernike defocus term. Measurements were acquire...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2017/4165706
更新日期：2017-01-01 00:00:00
abstract：:Objective. The purpose of this study was to examine how patient, physician, and situational factors are associated with the extent to which providers educate patients about glaucoma and glaucoma medications, and which patient and provider characteristics are associated with whether providers educate patients about gla...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2014/238939
更新日期：2014-01-01 00:00:00
abstract：Aim:The purpose of this study is to evaluate and compare the efficacy of 4 prostaglandin analogues (PGAs) and to determine the incidence of ocular surface disease in newly diagnosed, primary open-angle glaucoma (POAG) patients started on one of those 4 PGAs: bimatoprost (benzalkonium chloride, BAK, 0.3 mg/mL), latanopr...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2018/1319628
更新日期：2018-03-07 00:00:00
abstract：:Objective. Compare glaucoma medication costs between the United States (USA) and Canada. Methods. We modelled glaucoma brand name and generic medication annual costs in the USA and Canada based on October 2013 Costco prices and previously reported bottle overfill rates, drops per mL, and wastage adjustment. We also ca...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2015/547960
更新日期：2015-01-01 00:00:00
abstract：Purpose:To evaluate the clinical classification and characteristics of hereditary optic neuropathy patients in a single center in China. Method:Retrospective case study. Patients diagnosed with hereditary optic neuropathy between January 2014 and December 2015 in the neuro-ophthalmology division in Shanghai Eye and EN...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2017/6186052
更新日期：2017-01-01 00:00:00
abstract：Objective:In a group case series, the clinical characteristics of congenital membranous cataract in children were studied to establish a system of classification and determine the surgical method suited for each type. Methods:Children (18 eyes) with congenital membranous cataract were examined by slit lamp, ultrasound...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2017/2370969
更新日期：2017-01-01 00:00:00
abstract：:Aim. To evaluate the possible structural and functional changes in the retinal nerve fibre layer (RNFL) and the ganglion cell complex (GCC) of chronic smokers and compare them with those of passive healthy smokers using spectral domain optical coherence tomography (SD-OCT) and pattern electroretinogram (PERG). Materia...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2017/6354025
更新日期：2017-01-01 00:00:00
abstract：:Purpose. To compare the surgically induced astigmatism from clear corneal and square posterior limbal incisions at the time of cataract surgery. Methods. Surgically induced astigmatism was calculated for a set of eyes after cataract surgery using a temporal 2.2 mm square posterior limbal incision. Results were compare...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2011/243170
更新日期：2011-01-01 00:00:00
abstract：:Diabetic retinal complications, including macular edema (DME) and proliferative diabetic retinopathy (PDR), are the leading cause of new cases of blindness among adults aged 20-74. Chronic hyperglycemia, considered the underlying cause of diabetic retinopathy, is thought to act first through violation of the pericyte-...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2012/209538
更新日期：2012-01-01 00:00:00
abstract：Objective:This study aims to describe the clinical manifestations and outcomes in a cohort of older Chinese patients. Method:A retrospective study of patients aged ≥ 45 years who had a first episode of optic neuritis (ON) between May 2008 and November 2012. Clinical features at onset and last follow-up were analyzed w...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2017/3458356
更新日期：2017-01-01 00:00:00
abstract：Purpose:The purpose of this study was to evaluate 2-year visual outcomes in patients with diabetic macular edema (DME) treated with anti-VEGF agents in a routine clinical setting. Methods:The medical records of patients treated with ranibizumab or aflibercept due to DME at the Eye Hospital, University Medical Centre L...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2020/6979758
更新日期：2020-03-16 00:00:00
abstract：:Central retinal vein occlusion (CRVO) is a common retinal disease. Recent works mentioned spontaneous retinal arterial pulsations (SRAPs) as a feature of some CRVOs. This is a retrospective study on patients presenting with CRVO who were followed up for at least 6 months. The objective was to identify SRAP in the acut...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2020/3107472
更新日期：2020-06-09 00:00:00
abstract：:Purpose. To review the newer treatments for bacterial keratitis. Data Sources. PubMed literature search up to April 2012. Study Selection. Key words used for literature search: "infectious keratitis", "microbial keratitis", "infective keratitis", "new treatments for infectious keratitis", "fourth generation fluoroquin...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2012/831502
更新日期：2012-01-01 00:00:00
abstract：:Purpose. This study investigated the effects of oral propolis on the progression of galactose-induced sugar cataracts in rats and the in vitro effects of propolis on high-glucose-induced reactive oxygen species (ROS) and cell death in cultured rat lens cells (RLECs). Methods. Galactose-fed rats and RLECs cultured in h...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2016/1917093
更新日期：2016-01-01 00:00:00
abstract：Purpose:Visual loss is a devastating perioperative complication that can result from elevated intraocular pressure (IOP). The Trendelenburg position during surgery increases IOP. The purpose of this study was to quantify IOP changes in patients undergoing laparoscopic hysterectomy, at different time points and body pos...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2018/1457851
更新日期：2018-03-15 00:00:00
abstract：:We aimed to investigate the efficacy of frequency doubling technology (FDT) perimetry for glaucoma detection in comprehensive screening examinations. We performed a retrospective analysis of prospectively collected data of participants who underwent a comprehensive health checkup service. Participants with glaucoma we...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2020/4687398
更新日期：2020-03-29 00:00:00
abstract：Introduction:To assess long-term efficacy of bimedial rectus tendon elongation with Tutopatch in thyroid-associated orbitopathy (TAO). Materials and Methods:Retrospective chart review of 5 patients with TAO undergoing bimedial rectus recession with Tutopatch tendon elongation between 2009 and 2015. We analyzed horizon...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2018/1294761
更新日期：2018-07-24 00:00:00
abstract：Purpose:Glaucoma is the leading cause of irreversible blindness throughout the world. The pathogenesis of glaucoma is complex, and neuroprotection is a crucial aspect of therapy. High concentrations of extracellular glutamate are toxic to the optic nerve. The glutamate-aspartate transporter (GLAST) in retinal Müller ce...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2018/6128470
更新日期：2018-10-31 00:00:00
abstract：Purpose:Bilateral choroidal ganglioneuroma is extremely rare, and no cases have been described in the literature. Multimodal images are crucial for its diagnosis. Here, we evaluated multimodal images in the early stage of choroidal ganglioneuroma. Methods:A 6-year-old boy was recruited who had experienced gradually pr...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2020/6231269
更新日期：2020-05-11 00:00:00
abstract：:Aim. To assess the effects of mydriatics commonly used in clinical practice on choroidal thickness and anterior chamber change. Methods. This was a prospective, randomized, controlled, double-blinded study including a single eye of the participants. The subjects were assigned into 4 groups to receive tropicamide 1%, p...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2015/981274
更新日期：2015-01-01 00:00:00
abstract：:Purpose. To compare the corneal endothelial cell loss after phacoemulsification, alone or combined with microinvasive glaucoma surgery (MIGS), in nonglaucomatous versus primary open angle glaucoma (POAG) eyes affected by age-related cataract. Methods. 62 eyes of 62 patients were divided into group 1 (n = 25, affected ...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2015/769289
更新日期：2015-01-01 00:00:00
abstract：Purpose:The iStent provides a direct pathway for aqueous outflow from the anterior chamber to Schlemm's canal in patients with open-angle glaucoma (OAG). We performed a meta-analysis to evaluate the effectiveness of iStent as a standalone operation in patients with OAG in reducing the intraocular pressure (IOP) and the...
journal_title：Journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1155/2020/8754730
更新日期：2020-05-24 00:00:00
abstract：Purpose:To explore whether bandage lenses could be a safe and effective substitute for eye patch in the postoperative care for cataract surgery patients in terms of infection prevention, ocular impacts, and patient satisfaction. Methods:Patients who underwent cataract surgery were randomly divided into the eye patch g...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2018/1493967
更新日期：2018-05-15 00:00:00
abstract：Purpose:The purpose of this meta-analysis was to assess the percent reduction in the intraocular pressure (IOP) after argon laser peripheral iridoplasty (ALPI) and systemic medical therapy in patients with acute primary angle closure (APAC). Methods:We searched a number of electronic databases, including MEDLINE, EMBA...
journal_title：Journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1155/2019/7697416
更新日期：2019-05-02 00:00:00
abstract：:Macular pseudoholes (MPHs) and lamellar macular holes (LMHs) have been recently defined according to spectral domain optical coherence tomography (SD-OCT) criteria. A major feature for differentiating an MPH from an LMH remains the loss of foveal tissue. The anatomy of the foveola is peculiar with the macular pigment ...
journal_title：Journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1155/2019/4948212
更新日期：2019-02-10 00:00:00
abstract：Purpose:To assess the effect of age on elevation and pachymetric Pentacam keratoconus (KC) detection indices, and the need to adjust normative values accordingly. Methods:In a retrospective study, 95 eyes of myopic normal subjects without KC were evaluated using the OCULUS Pentacam, with an age range of 17.4 to 46.8 y...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2018/2016564
更新日期：2018-04-22 00:00:00
abstract：Purpose:To investigate the association between regional solar radiation and myopia occurrence in South Korean children. Materials and Methods:A population-based cross-sectional study using data of 1218 children aged 7-9 years from the Korea National Health and Nutritional Examination Survey was conducted from January ...
journal_title：Journal of ophthalmology
pub_type： 杂志文章
doi：10.1155/2019/7643850
更新日期：2019-06-10 00:00:00
