Abstract：
:Peste des petits ruminants (PPR) is a highly contagious and devastating viral disease affecting mainly sheep and goats, but also a large number of wild species within the order Artiodactyla. A better understanding of PPR transmission dynamics in multi-host systems is necessary to efficiently control the disease, in particular where wildlife and livestock co-occur. Notably, the role of wildlife in PPR epidemiology is still not clearly understood. Non-invasive strategies to detect PPR infection without the need for animal handling could greatly facilitate research on PPR epidemiology and management of the disease in atypical hosts and in complex field situations. Here, we describe optimized methods for the direct detection of PPR virus genetic material and antigen in fecal samples. We use these methods to determine the detection window of PPR in fecal samples, and compare the sensitivity of these methods to standard invasive sampling and PPR diagnostic methods using field samples collected at a wildlife-livestock interface in Africa. Our results show that quantitative reverse transcription PCR (RT-QPCR) amplification of PPRV from fecal swabs has good sensitivity in comparison to ocular swabs. Animals infected by PPRV could be identified relatively early on and during the whole course of infection based on fecal samples using RT-QPCR. Partial gene sequences could also be retrieved in some cases, from both fecal and ocular samples, providing important information about virus origin and relatedness to other PPRV strains. Non-invasive strategies for PPRV surveillance could provide important data to fill major gaps in our knowledge of the multi-host PPR epidemiology.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bataille A,Kwiatek O,Belfkhi S,Mounier L,Parida S,Mahapatra M,Caron A,Chubwa CC,Keyyu J,Kock R,Jones BA,Libeau Gdoi
10.1038/s41598-019-41232-ysubject
Has Abstractpub_date
2019-03-18 00:00:00pages
4742issue
1issn
2045-2322pii
10.1038/s41598-019-41232-yjournal_volume
9pub_type
杂志文章abstract：:Co-existing infection/inflammation and birth asphyxia potentiate the risk of developing neonatal encephalopathy (NE) and adverse outcome. In a newborn piglet model we assessed the effect of E. coli lipopolysaccharide (LPS) infusion started 4 h prior to and continued for 48 h after hypoxia on brain cell death and syste...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46488-y
更新日期：2019-07-15 00:00:00
abstract：:Malaria-associated acute lung injury (ALI) is a frequent complication of severe malaria that is often caused by "excessive" immune responses. To better understand the mechanism of ALI in malaria infection, here we investigated the roles of galectin (Gal)-1, 3, 8, 9 and the receptors of Gal-9 (Tim-3, CD44, CD137, and P...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32024
更新日期：2016-08-24 00:00:00
abstract：:Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07244-2
更新日期：2017-07-31 00:00:00
abstract：:Monosodium Glutamate (MSG) is used as flavour enhancer, with potential beneficial effects due to its nutritional value. Given the decline in kidney functions during aging, we investigated the impact of MSG voluntary intake on the kidney of male mice, aged 6 or 18 months. For 2 months, they freely consumed water (contr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80048-z
更新日期：2021-01-12 00:00:00
abstract：:We have developed a framework to convert an arbitrary integer factorization problem to an executable Ising model by first writing it as an optimization function then transforming the k-bit coupling (k ≥ 3) terms to quadratic terms using ancillary variables. Our resource-efficient method uses [Formula: see text] binary...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36058-z
更新日期：2018-12-05 00:00:00
abstract：:Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, we use microwestern array to rapidly evaluate suitable conditions for quantitative western blotting, with up to 192 antibody/dilution/replicate combinations on a single standard size gel with a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29436-0
更新日期：2018-07-27 00:00:00
abstract：:Natural sensory signals have nonlinear structures dynamically composed of the carrier frequencies and the variation of the amplitude (i.e., envelope). How the human brain processes the envelope information is still poorly understood, largely due to the conventional analysis failing to quantify it directly. Here, we us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53286-z
更新日期：2019-11-15 00:00:00
abstract：:Magnetic carboxyl functional nanoporous polymer (MCFNP) was chemically fabricated by incorporation of magnetic Fe3O4 precursor into the carboxyl functional nanoporous polymer (CFNP). The as-synthesized MCFNP was characterized and used as an adsorbent for rapid adsorption removal of methylene blue (MB) from wastewater....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24873-3
更新日期：2018-04-25 00:00:00
abstract：:We investigate theoretically the two-photon absorption (TPA) for circular graphene quantum dots (GQDs) with the edge of armchair and zigzag on the basis of electronic energy states obtained by solving the Dirac-Weyl equation numerically under finite difference method. The expressions for TPA cross section are derived ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33260
更新日期：2016-09-15 00:00:00
abstract：:Chromosome segment substitution lines (CSSLs) are useful tools for precise mapping of quantitative trait loci (QTLs) and the evaluation of gene action and interaction in inter-specific crosses. In this study, a set of 90 back cross lines at BC2F8 generation derived from Swarna x Oryza nivara IRGC81832 was evaluated fo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64300-0
更新日期：2020-05-08 00:00:00
abstract：:Models of biological processes are often subject to different sources of noise. Developing an understanding of the combined effects of different types of uncertainty is an open challenge. In this paper, we study a variant of the susceptible-infective-recovered model of epidemic spread, which combines both agent-to-age...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12606-x
更新日期：2017-10-11 00:00:00
abstract：:The temporomandibular muscle dysfunction is characterized by myofascial pain and is more prevalent in women of reproductive age. Sex steroid hormones are hypothetically involved in the dysfunction, but few are the studies of steroid receptors in masticatory and mastication-related muscles. Our aim was to determine est...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54774-y
更新日期：2019-12-05 00:00:00
abstract：:Gelatin-methacryloyl (GelMA) is one of the most commonly used photopolymerizable biomaterials in bio-applications. However, GelMA synthesis remains suboptimal, as its reaction parameters have not been fully investigated. The goal of this study is to establish an optimal route for effective and controllable GelMA synth...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31036
更新日期：2016-08-09 00:00:00
abstract：:Regulatory T cells (Tregs) expand in peripheral lymphoid organs and can produce immunosuppressive cytokines to support tumor growth. IL-10 abrogation efficiently induces Treg formation but dampens tumoral neuropilin-1 (Nrp-1) Treg signaling, which simultaneously augments Th1 and Th17 immunity. These effects are associ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24249
更新日期：2016-04-14 00:00:00
abstract：:We report the phonon and magnetic properties of various well-stabilized Co3O4 nanoparticles. The net valence in cobalt (II)/(III) cation can be obtained by subtracting the Co2+ ions in tetrahedral interstices and Co3+ ions in the octahedral interstices, respectively, which will possess spatial inhomogeneity of its mag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18563-9
更新日期：2018-01-10 00:00:00
abstract：:The extended Hückel (eH) tight-binding method has historically been prized for its computational ease and intuitive chemical clarity. However, its lack of quantitative predictiveness has prevented the eH method from being used as a tool for rapidly screening materials for desired electronic properties. In this work, w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28864-2
更新日期：2018-07-12 00:00:00
abstract：:In this study we present an image analysis methodology capable of quantifying morphological changes in tissue collagen fibril organization caused by pathological conditions. Texture analysis based on first-order statistics (FOS) and second-order statistics such as gray level co-occurrence matrix (GLCM) was explored to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02190
更新日期：2013-01-01 00:00:00
abstract：:Autism spectrum disorder involves persistent difficulties in social communication. Although these difficulties affect both verbal and nonverbal communication, there are no quantitative behavioral studies to date investigating the cross-modal coordination of verbal and nonverbal communication in autism. The objective o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54587-z
更新日期：2019-12-04 00:00:00
abstract：:We investigated the association between impulsivity related traits and BMI at the observational and genetic epidemiology level in a cross-sectional population of healthy young American-European adults. We studied 998 students and university staff of European ancestry recruited from Chicago (Illinois) and Athens (Georg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53922-8
更新日期：2019-11-26 00:00:00
abstract：:Tunicates are marine invertebrates that compose the closest phylogenetic group to the vertebrates. These chordates present a particularly diverse range of regenerative abilities and life-history strategies. Consequently, tunicates provide an extraordinary perspective into the emergence and diversity of these traits. H...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23749-w
更新日期：2018-04-03 00:00:00
abstract：:The CRISPR/Cas9 system is an efficient and convenient tool for genome editing in plants. Cas9 nuclease derived from Streptococcus pyogenes (Sp) is commonly used in this system. Recently, Staphylococcus aureus Cas9 (SaCas9)-mediated genome editing was reported in human cells and Arabidopsis. Because SaCas9 (1053 a.a.) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26871
更新日期：2016-05-26 00:00:00
abstract：:The prognostic value of current multigene assays for breast cancer is limited to hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer. Despite the prognostic significance of immune response-related genes in breast cancer, immune gene signatures have not been incorporated int...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80274-5
更新日期：2021-01-08 00:00:00
abstract：:To develop a clinic-relevant protocol for systemic up-regulation of NFE2-related factor 2 (Nrf2) to prevent diabetic cardiomyopathy (DCM), male db/db and age-matched wild-type (WT) mice were given sulforaphane (SFN, an Nrf2 activator) and its natural source, broccoli sprout extract (BSE) by gavage every other day for ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30252
更新日期：2016-07-26 00:00:00
abstract：:The conformation of the German shepherd dog (GSD) varies considerably within the breed. These differences may result in large variation in the movement and limb loading and undesirable consequences to their musculoskeletal health. This study aimed to investigate the relationship between conformation and biomechanical ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73550-x
更新日期：2020-10-15 00:00:00
abstract：:HIV-infected subjects under virological control still exhibit a persistent proinflammatory state. Thus, chronic HIV infection changes the host homeostasis towards an adapted immune response that may affect the outcome of coinfections. However, little is known about the impact of HIV infection on inflammatory amplifica...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43275-7
更新日期：2019-05-08 00:00:00
abstract：:While a large body of research has focused on the study of functional brain "connectivity", few investigators have focused on directionality of brain-brain interactions which, in spite of the mostly bidirectional anatomical substrates, cannot be assumed to be symmetrical. We employ a multivariate Granger Causality-bas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23996-x
更新日期：2018-04-03 00:00:00
abstract：:Microalgae and plankton can be a rich source of bioactivity. However, induction of secondary metabolite production in lab conditions can be difficult. One simple way of bypassing this issue is to collect biomass in the field and screen for bioactivity. Therefore, bulk net samples from three areas along the coast of no...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15980-8
更新日期：2017-11-20 00:00:00
abstract：:Osteoporosis is a disease characterized by low bone mass and progressive destruction of bone microstructure, resulting in increased the risk of fracture. Previous studies have demonstrated the effect of naringin (NG) or treadmill exercise (EX) on osteoporosis, however, reports about effects of NG plus EX on osteoporos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13009
更新日期：2015-08-11 00:00:00
abstract：:Next Generation Sequencing is dramatically increasing the number of known protein sequences, with related experimentally determined protein structures lagging behind. Structural bioinformatics is attempting to close this gap by developing approaches that predict structure-level characteristics for uncharacterized prot...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36679
更新日期：2016-11-18 00:00:00
abstract：:In the phytopathogenic basidiomycete Ustilago maydis, the Num1 protein has a pivotal function in hyphal morphogenesis. Num1 functions as a core component of the spliceosome-associated Prp19/CDC5 complex (NTC). The interaction of Num1 with the kinesin motor Kin1 suggests a connection between a component of the splicing...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21628-y
更新日期：2018-02-26 00:00:00
