Abstract:
:The CRISPR/Cas9 system is an efficient and convenient tool for genome editing in plants. Cas9 nuclease derived from Streptococcus pyogenes (Sp) is commonly used in this system. Recently, Staphylococcus aureus Cas9 (SaCas9)-mediated genome editing was reported in human cells and Arabidopsis. Because SaCas9 (1053 a.a.) is smaller than SpCas9 (1368 a.a.), SaCas9 could have substantial advantages for delivering and expressing Cas9 protein, especially using virus vectors. Since the protospacer adjacent motif (PAM) sequence of SaCas9 (5'-NNGRRT-3') differs from that of SpCas9 (5'-NGG-3'), the use of this alternative Cas9 nuclease could expand the selectivity at potential cleavage target sites of the CRISPR/Cas9 system. Here we show that SaCas9 can mutagenize target sequences in tobacco and rice with efficiencies similar to those of SpCas9. We also analyzed the base preference for 'T' at the 6th position of the SaCas9 PAM. Targeted mutagenesis efficiencies in target sequences with non-canonical PAMs (5'-NNGRRV-3') were much lower than those with a canonical PAM (5'-NNGRRT-3'). The length of target sequence recognized by SaCas9 is one or two nucleotides longer than that recognized by SpCas9. Taken together, our results demonstrate that SaCas9 has higher sequence recognition capacity than SpCas9 and is useful for reducing off-target mutations in crop.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kaya H,Mikami M,Endo A,Endo M,Toki Sdoi
10.1038/srep26871subject
Has Abstractpub_date
2016-05-26 00:00:00pages
26871issn
2045-2322pii
srep26871journal_volume
6pub_type
杂志文章abstract::The current research on cellular heat stress management focuses on the roles of heat shock proteins (HSPs) and the proteostasis network under severe stress conditions. The mild, fever-type stress and the maintenance of membrane homeostasis are less well understood. Herein, we characterized the acute effect of mild, fe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15821-8
更新日期:2017-11-15 00:00:00
abstract::The semantic variant of primary progressive aphasia (svPPA) is a clinical syndrome characterized by neurodegeneration and progressive loss of semantic knowledge. Unlike many other forms of frontotemporal lobar degeneration (FTLD), svPPA has a highly consistent underlying pathology composed of TDP-43 (a regulator of RN...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46415-1
更新日期:2019-07-26 00:00:00
abstract::Known susceptibility loci together can only explain about 6-8% of the disease heritability of IgA nephropathy (IgAN), suggesting that there are still a large number of genetic variants remained to be discovered. We previously identified IgAN and systemic lupus erythematosus (SLE)/lupus nephritis (LN) shared many loci ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35781
更新日期:2016-11-02 00:00:00
abstract::Photoluminescence and photomodulated reflectivity measurements of ZnOSe alloys are used to demonstrate a splitting of the valence band due to the band anticrossing interaction between localized Se states and the extended valence band states of the host ZnO matrix. A strong multiband emission associated with optical tr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44214
更新日期:2017-03-13 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-59358-9
更新日期:2020-02-05 00:00:00
abstract::Thermodynamics is a branch of science blessed by an unparalleled combination of generality of scope and formal simplicity. Based on few natural assumptions together with the four laws, it sets the boundaries between possible and impossible in macroscopic aggregates of matter. This triggered groundbreaking achievements...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03949
更新日期:2014-02-04 00:00:00
abstract::Islet transplantation is a novel promising strategy to cure type 1 diabetes. However, the long-term outcome is still poor, because both function and survival of the transplant decline over-time. Non-invasive imaging methods have the potential to enable monitoring of islet survival after transplantation and the effects...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15521
更新日期:2015-10-22 00:00:00
abstract::The liver has a variety of functions for maintaining homeostasis, and hepatocytes play a major role. In contrast with the high regenerative capacity of mature hepatocytes (MHs) in vivo, they have not been successfully expanded ex vivo. Here we demonstrate that CD44-positive cells sorted from small hepatocyte (SH) colo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46177
更新日期:2017-04-11 00:00:00
abstract::Headache is a common reason to visit the emergency department (ED). Tension-type headache (TTH) is the commonest headache. The diagnosis of TTH implies a mild condition, with no need for special tests. We evaluated the use of the International Classification of Headache Disorders (ICHD) criteria for TTH in the ED. We ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59171-4
更新日期:2020-02-12 00:00:00
abstract::In the present work, linoleic acid and oleic acid were isolated from Indonesian corn oil and palm oil and they were used to prepare monoacylglycerol derivatives as the antibacterial agent. Indonesian corn oil contains 57.74% linoleic acid, 19.88% palmitic acid, 11.84% oleic acid and 3.02% stearic acid. While Indonesia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47373-4
更新日期:2019-07-29 00:00:00
abstract::P-type doping in high Al-content AlGaN alloys is a main challenge for realizing AlGaN-based deep ultraviolet optoelectronics devices. According to the first-principles calculations, Mg activation energy may be reduced so that a high hole concentration can be obtained by introducing nanoscale (AlN)5/(GaN)1 superlattice...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44223
更新日期:2017-03-14 00:00:00
abstract::Bisacylphosphane oxides (BAPOs) are established as photoinitiators for industrial applications. Light irradiation leads to their photolysis, producing radicals. Radical species induce oxidative stress in cells and may cause cell death. Hence, BAPOs may be suitable as photolatent cytotoxic agents, but such applications...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42026-y
更新日期:2019-04-12 00:00:00
abstract::Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08463-3
更新日期:2017-08-10 00:00:00
abstract::Guanine-rich quadruplex (G-QD) are formed by conversion of nucleotides with specific sequences by stabilization of positively charged K+ or Na+. These G-QD structures differentially absorb two-directional (right- and left-handed) circularly polarized light, which can discriminate the parallel or anti-parallel structur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77449-5
更新日期:2020-11-24 00:00:00
abstract::We report that misfolded cytosolic proteins can be cleared from mammalian cells by directing them to endoplasmic reticulum (ER). NAT1 R64W and Parkin R42P are naturally occurring misfolded variants of cytosolic enzymes that acetylate arylamines and ubiquitinate proteins, respectively. We demonstrate that proteasome in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12088
更新日期:2015-07-14 00:00:00
abstract::Geographical origin determination of white rice has become the major issue of food industry. However, there is still lack of a high-throughput method for rapidly and reproducibly differentiating the geographical origins of commercial white rice. In this study, we developed a method that employed lipidomics and deep le...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08892-0
更新日期:2017-08-17 00:00:00
abstract::Networks of protoplasmic tubes of organism Physarum polycehpalum are macro-scale structures which optimally span multiple food sources to avoid repellents yet maximize coverage of attractants. When data are presented by configurations of attractants and behaviour of the slime mould is tuned by a range of repellents, t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19948
更新日期:2016-02-03 00:00:00
abstract::The endosymbiotic bacterium Wolbachia spreads rapidly through populations of Aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and Zika viruses. Mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16045-6
更新日期:2017-11-16 00:00:00
abstract::The prognosis of pancreatic cancer remains dismal despite continuous and considerable efforts. Integrins (ITGs) are highly expressed in various malignant cancers. However, very few studies investigated the role of integrin α3 (ITGα3) in malignant cancers. Here, we determined the functional role of ITGα3 in pancreatic ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39628-x
更新日期:2019-02-26 00:00:00
abstract::Open-ended evolution (OEE) is relevant to a variety of biological, artificial and technological systems, but has been challenging to reproduce in silico. Most theoretical efforts focus on key aspects of open-ended evolution as it appears in biology. We recast the problem as a more general one in dynamical systems theo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00810-8
更新日期:2017-04-20 00:00:00
abstract::To develop a clinic-relevant protocol for systemic up-regulation of NFE2-related factor 2 (Nrf2) to prevent diabetic cardiomyopathy (DCM), male db/db and age-matched wild-type (WT) mice were given sulforaphane (SFN, an Nrf2 activator) and its natural source, broccoli sprout extract (BSE) by gavage every other day for ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30252
更新日期:2016-07-26 00:00:00
abstract::The causes underlying sex differences in lifespan are strongly debated. While females commonly outlive males in humans, this is generally less pronounced in societies before the demographic transition to low mortality and fertility rates. Life-history theory suggests that reduced reproduction should benefit female lif...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24672
更新日期:2016-04-18 00:00:00
abstract::We report the experimental observation of energetically confined self-accelerating optical beams propagating along various convex trajectories. We show that, under an appropriate transverse compression of their spatial spectra, these self-accelerating beams can exhibit a dramatic enhancement of their peak intensity an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13197
更新日期:2015-08-24 00:00:00
abstract::MicroRNAs are a class of small noncoding RNAs that regulate gene expression post-transcriptionally either by inhibiting protein translation or by causing the degradation of target mRNAs. Current evidence indicates that miR-33b is involved in the regulation of lipid metabolism, cholesterol homeostasis, glucose metaboli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09995
更新日期:2015-04-28 00:00:00
abstract::Molecular taxonomy often uncovers cryptic species, reminding us that taxonomic incompleteness is even more severe than previous thought. The importance of cryptic species for conservation is poorly understood. Although some cryptic species may be seriously threatened or otherwise important, they are rarely included in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02938-z
更新日期:2017-06-13 00:00:00
abstract::The advent of random start protocols to shorten the time needed to store oocytes in women with malignancies has represented an important improvement in the field of fertility preservation. However, Randomized Controlled Trials are difficult to implement in this area and available evidence that supports this approach r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67151-x
更新日期:2020-06-22 00:00:00
abstract::The use of micro- and nanodevices as multifunctional systems for biomedical applications has experienced an exponential growth during the past decades. Although a large number of studies have focused on the design and fabrication of new micro- and nanosystems capable of developing multiple functions, a deeper understa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11371
更新日期:2015-06-12 00:00:00
abstract::E5 proteins are amongst the least understood of the Human Papillomavirus (HPV) encoded gene products. They are small, membrane-integrated proteins known to modulate a number of critical host pathways associated with pathogenesis including growth factor receptor signaling and immune evasion. Their role in the virus lif...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12523
更新日期:2015-08-03 00:00:00
abstract::The overuse of antibiotics results in the development of antibiotic resistance and limits the useful life of these drugs in fighting bacteria, including Aeromonas hydrophila, a well-known opportunistic pathogen that causes serious infections in fish and other animals. In this study, we investigated the adaptive resist...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39413
更新日期:2016-12-19 00:00:00
abstract::Identification of AD (Alzheimer's disease)-related genes obtained from blood samples is crucial for early AD diagnosis. We used three public datasets, ADNI, AddNeuroMed1 (ANM1), and ANM2, for this study. Five feature selection methods and five classifiers were used to curate AD-related genes and discriminate AD patien...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60595-1
更新日期:2020-02-26 00:00:00