Abstract:
:To investigate whether soluble Plasmodium falciparum antigens possess endotoxin-like properties, the interaction of Limulus amoebocyte lysate (LAL) with defined soluble antigens from Plasmodium falciparum was studied by various crossed immunoelectrophoretic methods and immunoblotting. The soluble P. falciparum antigens were purified by affinity chromatography using human IgG from malaria-immune adults as ligand. Of eight possible antigens, at least three were affected by LAL, as indicated by disappearance of these antigens in the precipitation pattern, after the reaction with LAL. One of the LAL-reactive antigens is a heat-stable glycoprotein with the presence of both hydrophilic and hydrophobic regions in its structure. This antigen shows a strong reaction with polymyxin B, and antibodies against it have been shown to be inhibitory to the growth of P. falciparum in culture. It is concluded that LAL reacts with several soluble antigens from P. falciparum and it is suggested that these antigens participate in the induction of protective immunity to malaria, and consequently that one or more of the soluble antigens are candidates for a malaria vaccine.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Jakobsen PH,Baek L,Jepsen Sdoi
10.1111/j.1365-3024.1988.tb00247.xsubject
Has Abstractpub_date
1988-11-01 00:00:00pages
593-606issue
6eissn
0141-9838issn
1365-3024journal_volume
10pub_type
杂志文章abstract::Fulani and Masaleit, sympatric tribes in eastern Sudan, are characterized by marked differences in susceptibility to Plasmodium falciparum malaria. To determine whether the two tribes differ in the frequency of immunoglobulin GM/KM allotypes, which are associated with immunity to several pathogens, serum samples from ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00938.x
更新日期:2007-05-01 00:00:00
abstract:AIMS:Merozoite surface protein 8 (MSP-8) of Plasmodium parasites plays an important role in erythrocyte invasion and is a potential malaria vaccine candidate. METHODS AND RESULTS:In this study, virus-like particles (VLPs) expressing MSP-8 of Plasmodium berghei on the surface of influenza virus matrix protein 1 (M1) co...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12781
更新日期:2020-11-01 00:00:00
abstract::Plasmodium falciparum-specific antibodies tend to be short-lived, but their cognate memory B cells (MBCs) circulate in the peripheral blood of exposed subjects for several months or years after the last infection. However, the time course of antigen-specific antibodies and B-cell responses to the relatively neglected ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12662
更新日期:2019-09-01 00:00:00
abstract::In this study, supplementation with the probiotic Saccharomyces boulardii promoted a reduction in intensity of infection by Toxocara canis and modulates cytokines mRNA expression in experimentally infected mice. IL-12 gene transcription had 40-fold increase in S. boulardii supplemented uninfected mice and sevenfold in...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12314
更新日期:2016-05-01 00:00:00
abstract::Gastrointestinal (GI) nematodes are a group of successful multicellular parasites that have evolved to coexist within the intestinal niche of multiple species. It is estimated that over 10% of the world's population are chronically infected by GI nematodes, making this group of parasitic nematodes a major burden to gl...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12517
更新日期:2018-04-01 00:00:00
abstract::Antibodies and B cells are critical in the protective immune response to the blood stage of the malaria parasite, Plasmodium chabaudi. However, little is known about the development of memory B cells and their differentiation into plasma cells during infection or after re-infection. Here we have shown that B cells wit...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2008.01066.x
更新日期:2009-01-01 00:00:00
abstract::Activation of macrophages is a key step in the initiation of immune responses, but the transcriptional mechanisms governing macrophage activation during infection are not fully understood. It was recently shown that the AP-1 family transcription factor JUNB positively regulates macrophage activation in response to Tol...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12215
更新日期:2015-09-01 00:00:00
abstract::Cysteine proteinase enriched fractions obtained by thiol-sepharose chromatography of Ostertagia ostertagi membrane-bound protein extract (S3-thiol) or total adult excretory-secretory (ES-thiol) products were tested in a vaccination experiment to evaluate their protective efficacy against O. ostertagi in cattle. Calves...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00461.x
更新日期:2002-05-01 00:00:00
abstract::Levels of intestinal mast cell protease (IMCP) were quantified in serum, gut tissue and in intestinal fluids taken from mice infected with Trichinella spiralis during primary and secondary infections. The ability to generate a mast cell response was dependent on the response phenotype of the mouse strain used. The mas...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00996.x
更新日期:1990-11-01 00:00:00
abstract::A monoclonal antibody (NIM-M7) has been prepared which reacts with a major surface antigen of adult males and females of Trichinella spiralis. This specificity is only demonstrable when the antigen is liberated by detergent solubilization of surface-labelled worms. When reacted with living adults, on the other hand, N...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00874.x
更新日期:1986-11-01 00:00:00
abstract::In vitro released products of the adult stage of the bovine lungworm. Dictyocaulus viviparus, were characterized according to their SDS-PAGE profile, glycosylation pattern, in vitro synthesis and antigenicity in the context of infection and vaccination with irradiated larvae. Biosynthetic labelling experiments with 35...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00596.x
更新日期:1993-03-01 00:00:00
abstract::Groups of five dogs were vaccinated against Babesia canis using soluble parasite (SPA) antigens from in vitro cultures. Although vaccination did not significantly alter peripheral parasitaemia upon challenge, protected animals had lower levels of SPA in the plasma after a challenge infection. The severity of anaemia c...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-1.x
更新日期:1996-01-01 00:00:00
abstract::Recent advances in molecular genetics and in imaging mean that it is now increasingly feasible to image biological processes within helminth parasites and to visualize interactions between worms and their hosts. Moreover, other innovative imaging approaches that are not dependent on transgenic parasites have been appl...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12051
更新日期:2013-09-01 00:00:00
abstract::Successful endoparasites of mammals must outwit the sophisticated immune systems of their hosts that have evolved to detect and destroy/eradicate them. Many species of helminth parasite can directly or indirectly manipulate host immunity: helminth-derived molecules can suppress or skew activity of many immune cell phe...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12160
更新日期:2015-06-01 00:00:00
abstract::The chromadorea abundant larval transcript (ALT) family of proteins contains ALT one of the most studied putative vaccine candidate in experimental filariasis. This study reports the characterization of Wuchereria bancrofti 20/22 (Wb20/22) as a member of chromadorea, the ALT family of proteins from the L3 stage of W. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12120
更新日期:2014-10-01 00:00:00
abstract::The purpose of this study was to monitor local cytokine responses to Dictyocaulus viviparus in calves during primary infection and re-infection. Bronchoalveolar lavage fluid (BALF) was collected weekly from experimentally infected calves and interleukin-2 (IL-2), IL-4, IL-5, IL-10 and IFN-γ mRNA expression was quantif...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12083
更新日期:2014-02-01 00:00:00
abstract::Changes in phagocytic and adherent cell numbers were compared during the course of infections of mice with Plasmodium yoelii (Py) and P. berghei (Pb) and in vaccinated mice challenged with homologous parasites. Nucleated cells in the spleen increased in number in Py-infected mice and were maximal at the time of recove...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1979.tb00696.x
更新日期:1979-04-01 00:00:00
abstract::Intraperitoneal injection of Trypanosoma brucei AnTat 1.1 into mice of the C3H.He, BALB/c or C57BL/6 strains resulted in impaired immune responses from day 3 onwards, as measured by the reduction in DNA synthesis in spleen cell populations stimulated with concanavalin A (Con-A) in vitro. Adherent cells from the perito...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00951.x
更新日期:1990-05-01 00:00:00
abstract::HIV and T. gondii infection markers were measured among 383 Intravenous Drug Users (IDU). And cytokine concentrations (IL-4, IL-6, IL-10, IL-12, IFN-gamma and TNF-alpha) were determined. The results showed IDU with HIV infection or HIV/T. gondii co-infection could disturb Th regulatory mechanism. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01174.x
更新日期:2010-02-01 00:00:00
abstract::We have assessed the role of tumour necrosis factor-alpha (TNF) during cutaneous leishmaniasis and demonstrated that significant levels of TNF were released by spleen cells from infected mice after in vitro restimulation with Leishmania major promastigotes. Spleen cells from both genetically resistant and genetically ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00983.x
更新日期:1990-09-01 00:00:00
abstract::Naegleria fowleri, a free-living amoeba, is the causative pathogen of primary amoebic meningoencephalitis in humans and experimental mice. N. fowleri is capable of destroying tissues and host cells through lytic necrosis. However, the mechanism by which N. fowleri induces host cell death is unknown. Electron microscop...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01297.x
更新日期:2011-07-01 00:00:00
abstract::In the present study, the cytokines interleukin (IL)-12 and IL-18 were evaluated for their capacity to modulate and to re-direct in vitro parasite antigen-specific cellular responsiveness in patients exposed to Onchocerca volvulus and Entamoeba histolytica infection. We found that IL-18 was highly capable of reducing ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2003.00638.x
更新日期:2003-06-01 00:00:00
abstract::Translationally controlled tumour protein (TCTP) may play an important role in the establishment or maintenance of parasitemia in a malarial infection. In this study, the potential of TCTP as a malaria vaccine was investigated in two trials. In the initial vaccine trial, Plasmodium falciparum TCTP (PfTCTP) was express...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12158
更新日期:2015-01-01 00:00:00
abstract::Entamoeba histolytica is known to induce host cell death via activation of calpain and caspases. In this study, we investigated the specific proteases involved in the degradation of cytoskeletal proteins during Jurkat T-cell death induced by E. histolytica. Amoebic trophozoites induced marked degradation of paxillin, ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01290.x
更新日期:2011-06-01 00:00:00
abstract::The humoral responses in mice following vaccination with DNA constructs encoding Fasciola hepatica glutathione S-transferase (GST) have been evaluated. GST47 cDNA was subcloned into two DNA vaccine vectors, VR1012 and VR1020, which direct expression to the cytoplasmic and extracellular compartments, respectively. Expr...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1999.00235.x
更新日期:1999-07-01 00:00:00
abstract::A surface enriched fraction was prepared from adults of Onchocerca volvulus by brief extraction of entire worms with detergent. This was then gel filtered to yield a low molecular weight fraction which functioned specifically in ELISA analysis. An identical result was also obtained when the related cattle parasite, O....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1987.tb00487.x
更新日期:1987-01-01 00:00:00
abstract::The pathogenesis of the tissue lesions observed in American trypanosomiasis (Chagas' disease) seems to depend on a variety of mechanisms. The present study was dedicated to the investigation of the evolution of muscle lesions and its relationship with immunological and parasitological parameters. Mice were infected i....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00091.x
更新日期:1985-09-01 00:00:00
abstract::Two monoclonal antibodies against the surface of S. mansoni schistosomula were found to confer significant passive protection to mice (M7B3A, range 28-70%; M22H12C, range 14-58%). No additive effect was observed when both were transferred together. Neither McAb bound to the cercarial surface but both bound to the surf...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00836.x
更新日期:1986-01-01 00:00:00
abstract::The efficacy of vaccination against Haemonchus contortus infection with two recombinant proteins, rHco-gal-m and rHco-gal-f, was studied in 9-10-month-old goats. Vaccination with 100 microg protein reduced faecal egg output and worm burdens by 37.25% and 41.1%, respectively. Corresponding reductions with 200 microg pr...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00949.x
更新日期:2007-06-01 00:00:00
abstract::The life cycle of Taenia solium, the pork tapeworm, is continuously closed in many rural settings in developing countries when free roaming pigs ingest human stools containing T. solium eggs and develop cysticercosis, and humans ingest pork infected with cystic larvae and develop intestinal taeniasis, or may also acci...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12126
更新日期:2014-08-01 00:00:00