Abstract：
Aim of Study:The roles of autophagy performed in chemotherapy-induced cell death or proliferation inhibition were still in debate. In this study, we aimed to disclose the function of autophagy in chemotherapy of HCT-116 colon cells. Materials and Methods:Pharmacological and genetic methods were applied to induce and inhibit autophagy and elucidate the roles of autophagy performed in chemotherapy-induced proliferation inhibition and apoptosis. Autophagy was assessed by microtubule-associated protein light chain 3 (LC3) expression and monodansylcadaverine (MDC) staining. Results:After treatment with 5-fluorouracil (5-FU), HCT-116 cells showed typical autophagy as stained by MDC. Autophagy inhibitor (3-methyladenine [3-MA]) or inducer (rapamycin) was applied in combination with 5-FU, respectively. As evidenced by our data, 3-MA inhibited while rapamycin facilitated 5-FU-induced apoptosis and proliferation inhibition of HCT-116 cells. Consistently, 3-MA inhibited, while rapamycin facilitated 5-FU-induced expressions of Beclin1 and LC3B. Moreover, 3-MA inhibited while rapamycin facilitated 5-FU-induced p53 protein expression. Using genetic method, Beclin1 overexpression increased while Beclin1 knockdown decreased 5-FU-induced cell proliferation inhibition and apoptosis. Especially, Beclin1 overexpression increased while Beclin1 knockdown decreased 5-FU-induced p53 expression. Conclusion:Our study provides both of pharmacological and genetic evidence to support that autophagy facilitates anticancer effect of the chemotherapeutic agent. The associated application of autophagy inducer with 5-FU would be beneficial for the chemotherapy in HCT-116 cancer cells.
journal_name
J Cancer Res Therjournal_title
Journal of cancer research and therapeuticsauthors
Yang JW,Zhang QH,Liu Tdoi
10.4103/0973-1482.204898subject
Has Abstractpub_date
2018-12-01 00:00:00pages
S1141-S1147issue
Supplementeissn
0973-1482issn
1998-4138pii
JCanResTher_2018_14_12_1141_247191journal_volume
14pub_type
杂志文章abstract：:Primary amyloidosis of bladder is a rare disease that closely resembles bladder cancer on clinical presentation with painless gross hematuria. Pathologically this is a totally benign non-neoplastic lesion and its association with urothelial carcinoma of the bladder is rare. We herein report a 64-year-old diabetic male...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.98994
更新日期：2012-04-01 00:00:00
abstract：:Papillary carcinoma is a well-differentiated type of thyroid carcinoma with very good overall prognosis. The commonest site of metastases is the lymph node, distant metastases being rare. We present a case of a 52-year-old male who presented with a solitary metastasis in the left para-sellar region and cavernous sinus...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,评审
doi：10.4103/0973-1482.103528
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate whether there was an association between pathology type and ERCC1, BRCA1, RRM1, TUBB3, STMN1, TOP2A and epidermal growth factor receptor (EGFR) messenger ribonucleic acid (mRNA) expression level in Chinese patients with non-small cell lung carcinoma (NSCLC). MATERIALS AN...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.119118
更新日期：2013-09-01 00:00:00
abstract：Aims:This study aims at assessing the volume changes that occur in the targets (gross tumor volume and planning target volume [PTV]) and the organs at risk in squamous cell carcinoma of the head and neck during radiotherapy and assessing the dose changes that occur as a result of them. Settings and Design:This was a p...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_589_19
更新日期：2020-04-01 00:00:00
abstract：:Renal cell carcinoma is the most frequent kidney neoplasm, with a high tendency to metastasize. The occurrence of renal carcinoma metastasis to the head and neck region is extremely rare. Here we present one such case where the tongue metastasis was the initial presenting feature of disease. ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.110392
更新日期：2013-01-01 00:00:00
abstract：:Skin metastases, as a presenting symptom of gastrointestinal malignancies, are very rare and signify aggressive disease. They usually occur after a long period of diagnosis and along with other visceral metastases. We present the case of an 18-year-old male with diffuse subcutaneous metastases as a presenting feature ...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件
doi：10.4103/0973-1482.172586
更新日期：2018-04-01 00:00:00
abstract：Objective:Undesired neutron contamination imposed to patients during treatment is among the main factors increasing the risk of secondary cancer in radiotherapy. This additional undesirable dose is due to neutron contamination production in high-energy accelerators. In this study, neutron contamination is investigated ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_1257_16
更新日期：2019-03-01 00:00:00
abstract：:Heart metastasis of renal cell carcinoma (RCC) without vena cava inferior involvement is exceptionally rare. Here, we presented a case of RCC presenting with the right ventricle metastasis without inferior vena caval and right atrial involvement. ...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件
doi：10.4103/0973-1482.193111
更新日期：2018-01-01 00:00:00
abstract：Background:Ghrelin plays a role in mechanisms related to cancer progression - including cell proliferation, invasion and migration, and resistance to apoptosis in the cell lines from several cancers. We investigated the role of ghrelin levels in cancer cachexia-anorexia in patients with locally advanced nonsmall-cell l...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：110.4103/jcrt.JCRT_10_19
更新日期：2020-07-01 00:00:00
abstract：OBJECTIVE:Our experience with endoscopic surgery for vascular leiomyomas of the nasal septum was reviewed. PATIENTS AND METHODS:All patients with vascular leiomyomas of the nasal septum who were treated with endoscopic surgery from May 1995 to June 2007 were reviewed retrospectively. RESULTS:Twelve patients had vascu...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.150417
更新日期：2016-01-01 00:00:00
abstract：Objectives:Increasing evidence suggests that Wnt5a plays an important role in tumorigenesis. In particular, its expression is downregulated in hepatocellular carcinoma (HCC). The aim of this study was to explore the effect of Wnt5a overexpression on HCC cells. Materials and Methods:We transfected the human HCC cell li...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_886_18
更新日期：2019-01-01 00:00:00
abstract：:We herein describe a case of drug-induced interstitial lung disease (ILD) following treatment with erlotinib. The plasma trough concentration of erlotinib at the time of the ILD diagnosis was extremely elevated compared with the plasma maximum concentration on day 1. We hypothesized that this phenomenon was associated...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.95201
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Clinical significance of chloride intracellular channel 1 (CLIC1) in pancreatic ductal adenocarcinomas (PDAC) remains largely unknown. This study was performed to assess the expression of CLIC1 in benign and malignant pancreatic lesions, and to assess its clinicopathological significance. MATERIALS AND METH...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.154057
更新日期：2016-04-01 00:00:00
abstract：:The novel coronavirus (COVID-19) pandemic has disrupted healthcare delivery across the globe. Cancer patients are at a higher risk of acquiring this infection due to their immunosuppressed state. Timely care of patients while ensuring safety of healthcare workers is need of the hour. We list few precautionary measures...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_405_20
更新日期：2020-04-01 00:00:00
abstract：:Heat shock protein 90 (Hsp90) a member of the heat shock proteins (HSPs) family, is an adenosine triphosphate dependent molecular chaperone protein, which integrates multiple oncogenic pathways. Clinically, encouraging results have been demonstrated in melanoma, acute myeloid leukemia, castrate refractory prostate can...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,评审
doi：10.4103/0973-1482.126451
更新日期：2013-10-01 00:00:00
abstract：Objective:This study presents a method to estimate midplane dose (Diso, transit) in vivo from transit signal (St) measured with thimble ionization chamber in cancer of the middle-third esophagus patients treated with three-dimensional radiotherapy (RT). This detector is positioned at the level of electronic portal imag...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_278_17
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this meta-analysis was to evaluate the effects of Fufangkushen injection combined with chemotherapy in the treatment of stomach cancer. MATERIALS AND METHODS:The relevant clinical trials about Fufangkushen injection combined with chemotherapy in the treatment of stomach cancer were search in t...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,meta分析
doi：10.4103/0973-1482.139755
更新日期：2014-08-01 00:00:00
abstract：:Primary pulmonary epithelioid angiosarcoma is an extremely rare malignancy. Herein, we report the case of an elderly Chinese patient with primary pulmonary epithelioid angiosarcoma. The 72-year-old man presented with a 1-month history of persistent hemoptysis and left chest pain and weight loss of 3 kg. A chest comput...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件,评审
doi：10.4103/0973-1482.176419
更新日期：2018-06-01 00:00:00
abstract：:Prostate cancer, one of the most common male malignancies with an increasing incidence in the recent years, requires the development of new methods of treatment. One of the most debated subjects is the tumor-associated macrophages (TAM). Although, the pathophysiological mechanisms are still a subject of intense resear...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,评审
doi：10.4103/0973-1482.163692
更新日期：2017-07-01 00:00:00
abstract：Purpose:The purpose of the study is to retrospectively evaluate the safety and efficacy of microwave ablation (MWA) for the treatment of ground-glass opacity (GGO)-lung adenocarcinoma. Materials and Methods:From December 2013 to June 2017, a total of 51 patients (22 males and 29 females, mean age of 69.4 ± 10.1 years)...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_269_18
更新日期：2018-01-01 00:00:00
abstract：:Masseter hypertrophy is a characteristic condition resulting from an increase in the size of the muscle mass. At times, it produces significant facial asymmetry and is an important finding in the differential diagnosis of similar located entities. A proper diagnosis of this condition would avoid more aggressive and un...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件
doi：10.4103/0973-1482.204889
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVE:To study the clinical results and prognostic factors of patients with glioblastoma multiforme (GBM) treated by postoperative radiation therapy (PORT) and concomitant temozolomide followed by adjuvant temozolomide. METHODS:From 2005 to 2008, 215 patients (median age 48 years) with GBM were treated with PORT p...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.119310
更新日期：2013-07-01 00:00:00
abstract：CONTEXT:Toxoplasma gondii is a protozoan parasite with a world-wide distribution. However, the majority of infected cases remain symptomless. There are raising scientific evidences indicating that parasitic infections induce antitumor activity against certain types of cancers. The inhibitory effect of T. gondii on canc...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.144638
更新日期：2015-04-01 00:00:00
abstract：Background:The symptoms of patients with malignant biliary obstruction (MBO) could be effectively alleviated with percutaneous transhepatic biliary drainage (PTBD). Postoperative infections were considered as challenging issues for clinicians. In this study, the risk factors of biliary infection in patients after PTBD ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_256_18
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVE:The serum level of Cyfra21-1 was always elevated in patients with nonsmall cell lung carcinoma. The aim of this meta-analysis was to evaluate the serum Cyfra21-1 as a biomarker in the diagnosis of nonsmall cell lung cancer (NSCLC). METHODS:All the articles associated with serum Cyfra21-1 in the diagnosis of ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,meta分析
doi：10.4103/0973-1482.145835
更新日期：2014-11-01 00:00:00
abstract：Purpose:To evaluate the effectiveness and complications of intra-arterial chemotherapy (IAC) for treating advanced refractory retinoblastoma (RB) in a large single-center cohort. Patients and Methods:Eighty-four eyes of 62 consecutive patients with advanced refractory RB who received IAC were included in the study dur...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_722_17
更新日期：2018-01-01 00:00:00
abstract：Background:Nuclear factor kappa B (NFkB-light-chain-enhancer of activated B-cells) expression and its regulation is a key role in the development of number of malignancies, as NFkB mediates the balance between cell death and its survival. Therefore, NFkB regulation constitutes an attractive target to overcome the resis...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_348_18
更新日期：2018-12-01 00:00:00
abstract：:Follow-up of colorectal carcinoma after therapy is based on symptoms, tumor markers, and imaging studies. Clinicians sometimes face diagnostic dilemmas because of unusual presentations on the imaging modalities coupled with rising serum markers. We report a case of colorectal carcinoma that presented with gastrointest...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.42262
更新日期：2008-04-01 00:00:00
abstract：:Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gastrointestinal tract. Tyrosine kinase inhibitors (TKIs), such as imatinibmesylate, have been used for the adjuvant treatment of KIT-positive GISTs. Several pathologic changes after imatinib treatment of metastatic GIST including tumor ...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件,评审
doi：10.4103/0973-1482.136030
更新日期：2014-07-01 00:00:00
abstract：:Squamous cell carcinoma of urinary tract is a rarely encountered tumor. It is more frequently reported in urinary bladder and male urethra than renal pelvis. Squamous cell carcinoma of renal pelvis is usually associated with nephrolithiasis. However, coexistence of keratinizing squamous cell carcinoma with xanthogranu...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.73351
更新日期：2010-07-01 00:00:00
