Abstract：
:To identify anterior pituitary cell types containing GnRH binding sites and to study the internalization process of this peptide by target cells under physiological conditions, autoradiography was performed on rat anterior pituitaries removed at specific time intervals (2-60 min) after intravenous injection of mono-radioiodinated 125I-GnRH into intact males. At electron-microscopic level, gonadotrophs and lactotrophs appeared to contain silver grains. Concomitant administration of an excess of unlabeled GnRH with the radioiodinated hormone prevented this localization indicating the specificity of the reaction. The time-course study in gonadotrophs showed that 2 min after injection silver grains could be found over the plasma membrane, secretory granules and nuclear membrane. Similar results were observed 5 and 15 min after injection. Extensive label was observed over the nucleus and nuclear membrane 15 to 60 min after injection. The injection of a radioiodinated GnRH agonist [D-Trp6, Pro9 (Net), DesGly10]-GnRH produced comparable results. In contrast, the injection of 125I-[D-pGlu1, D-Phe2, Trp3,6]-GnRH, an antagonist of GnRH, produced positive labeling only at the plasma membrane without internalization. These results indicate that, after binding with receptors on the plasma membrane, GnRH is rapidly internalized, accumulating in secretory granules, and localizing over the nuclear membrane and later, in the nucleus. Association of radioactivity with secretory granules could be related to a specific action of GnRH at this level or to receptor recycling, and presence of label in the nucleus may be related to stimulation of neosynthesis of LH and GnRH receptors.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Morel G,Dihl F,Aubert ML,Dubois PMdoi
10.1007/BF00216482subject
Has Abstractpub_date
1987-06-01 00:00:00pages
541-50issue
3eissn
0302-766Xissn
1432-0878journal_volume
248pub_type
杂志文章abstract：:The colocalization of immunoreactivities to substance P and calcitonin gene-related peptide (CGRP) in nervous structures and their correlation with other peptidergic structures were studied in the stellate ganglion of the guinea pig by the application of double-labelling immunofluorescence. Three types of fibre were d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318563
更新日期：1993-06-01 00:00:00
abstract：:A somatostatin-like substance is demonstrated by light microscopic immunohistochemistry (PAP-method) in perikarya and cell processes of the retina of adult and infant rats. These perikarya are identified according to their size, arrangement and distribution. Each of the first two neuronal orders (receptors, bipolar ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00235169
更新日期：1979-01-01 00:00:00
abstract：:Box jellyfish have the most elaborate sensory system and behavioural repertoire of all cnidarians. Sensory input largely comes from 24 eyes situated on four club-shaped sensory structures, the rhopalia, and behaviour includes obstacle avoidance, light shaft attractance and mating. To process the sensory input and conv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0393-7
更新日期：2007-07-01 00:00:00
abstract：:The ultrastructure of the rectal papillae of the parasitoid hymenopteran, Nasonia vitripennis (Walk), is described. These organs in this insect consist of four distinct cell types arranged as a closed, hollow cone. The majority of the cells are present in the raised cone, and are characterised by large numbers of mito...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220008
更新日期：1975-08-25 00:00:00
abstract：:The development of the heterophil granulocyte in the bone marrow of the rat is described, and an electron-microscopical analysis of the changes in the cytoplasm as well as in the granule population in several stages of maturation is reported. Three types of granule originate in consecutive stages of heterophil maturat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00211466
更新日期：1983-01-01 00:00:00
abstract：:Light and electron microscopic studies, including the use of the vital dyes trypan blue and acridine orange, indicate that the topical application of precocene II rapidly triggers degenerative processes in the corpora allata (CA) leading within a few days to the virtual disappearance of the parenchymal cells of these ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234326
更新日期：1979-11-01 00:00:00
abstract：:Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219221
更新日期：1986-01-01 00:00:00
abstract：:Infection by influenza virus leads to respiratory failure characterized by acute lung injury associated with alveolar edema, necrotizing bronchiolitis, and excessive bleeding. Severe reactions to infection that lead to hospitalizations and/or death are frequently attributed to an exuberant host response, with excessiv...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1043-z
更新日期：2011-01-01 00:00:00
abstract：:Long-term in vitro culture (16 days) of caprine ovarian cortical tissue was performed to test the effect of FSH and IGF-I on the viability and development of preantral follicles and mRNA expression for FSH and IGF-I receptors. Fragments were cultured in α-MEM(+) alone or supplemented with different combinations of FSH...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1498-1
更新日期：2012-12-01 00:00:00
abstract：:We have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingl...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1224-4
更新日期：2011-09-01 00:00:00
abstract：:Modifications of hepatocyte cell surface were determined after single i.v. injection to rats of Pb(NO(3))(2) (known to induce liver hyperplasia followed by apoptosis) or GdCl(3) (known to induce proliferation of parenchymal cells and Kupffer cell depletion) or administration of GdCl(3) 24 h before Pb(NO(3))(2) injecti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0708-2
更新日期：2003-04-01 00:00:00
abstract：:Sudden death in athletes occurs because of the existence of hidden cardiovascular disorders which, during effort, may jeopardize the electrical stability of the heart, triggering ventricular tachycardia and/or fibrillation. Apart from rare conditions of ion channel diseases in the setting of a structurally normal hear...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1398-4
更新日期：2012-05-01 00:00:00
abstract：:The fine structure and primary sensory projections of sensilla located in the labial-palp pit organ of the cotton bollworm Helicoverpa armigera (Insecta, Lepidoptera) are investigated by scanning electron and transmission electron microscopy combined with confocal laser scanning microscopy. The pit organ located on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1657-z
更新日期：2013-09-01 00:00:00
abstract：:Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN + erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitocho...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214924
更新日期：1985-01-01 00:00:00
abstract：:Appearance of immunoreactive corticotropin-releasing factor (CRF)-containing neurons was studied in developing hypothalamus of the rat by use of antisera against rat- and ovine CRF. These neurons were first recognized in the lateral and paraventricular nuclei on days 15.5 and 16.5 of gestation, respectively, when anti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219870
更新日期：1984-01-01 00:00:00
abstract：:Gastrin stimulates gastric acid secretion by acting on the cholecystokinin B/gastrin receptor (CCK-BR). The localization of this receptor at the cellular level showed conflicting results in animal studies and has not been described in man by immunohistochemistry. The aim of the present study is to characterize the pre...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900114
更新日期：2000-02-01 00:00:00
abstract：:The effect of copulation on the rat coagulating gland (anterior prostate) was studied. At 4 to 6 h after the beginning of copulation the coagulating glands of rats that had produced copulatory plugs were nearly empty of secretion. Ultrastructurally, the coagulating gland has large cisternae of rough endoplasmic reticu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221773
更新日期：1977-07-19 00:00:00
abstract：:Transmission electron microscopy was used to study the effects of proteolytic enzymes (collagenase, trypsin, clostripain), the calcium chelator ethyleneglycol-bis-(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA), and the calcium ionophore A 23187 on substrate adhesion and fine structure of chondrocytes and fi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00228443
更新日期：1984-01-01 00:00:00
abstract：:The termination of intrafusal muscle fibres was studied in muscle spindles of the tenuissimus muscle of the Chinese hamster. Most nuclear chain fibres terminated within the spindle capsule. The nuclear bag- and nuclear chain fibres that extended beyond the limit of the spindle capsule terminated in the endomysial conn...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218663
更新日期：1983-01-01 00:00:00
abstract：:The purpose of this study was to determine the cellular distribution and degradation in rat liver following intravenous injection of superparamagnetic iron oxide nanoparticles used for magnetic resonance imaging (NC100150 Injection). Relaxometric and spectrophotometric methods were used to determine the concentration ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0884-8
更新日期：2004-06-01 00:00:00
abstract：:An increasingly pro-oxidant environment has been widely implicated in causing dysfunction of testicular steroidogenesis, but little progress has been made in understanding the underlying molecular mechanism. Here, we report that gamma-glutamyl transferase 5 (GGT5), a key metabolism component responsible for the cataly...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2458-y
更新日期：2016-11-01 00:00:00
abstract：:Recent studies demonstrated the expression of the insulin receptor (InsR) and its functional interaction with the transient receptor potential vanilloid type 1 receptor (TRPV1) in primary sensory neurons (PSNs). The present study was undertaken to reveal the target-specific expression of the InsR and its co-localizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2868-0
更新日期：2018-11-01 00:00:00
abstract：:Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305731
更新日期：1991-04-01 00:00:00
abstract：:Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327290
更新日期：1991-03-01 00:00:00
abstract：:Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2857-3
更新日期：2018-10-01 00:00:00
abstract：:Single- and dual-labelling immunohistochemistry were used to determine the distribution and coexistence of neuropeptides in perivascular nerves of the large arteries and veins of the snake, Elaphe obsoleta, using antibodies for vasoactive intestinal polypeptide, substance P, calcitonin gene-related peptide, neuropepti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00353904
更新日期：1992-09-01 00:00:00
abstract：:Acute necrotizing pancreatitis (ANP) is a common gastrointestinal cause of emergency admissions in dogs and humans and can lead to a systemic inflammatory response syndrome resulting in multiple organ dysfunction syndrome. Among the various complications associated with ANP, acute lung injury (ALI) or acute respirator...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03211-y
更新日期：2020-05-01 00:00:00
abstract：:In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215313
更新日期：1976-05-13 00:00:00
abstract：:Since the advent of transmission electron microscopy of tissues capable of growth and regeneration, cell and developmental biologists have postulated that the undifferentiated cells observed within these tissues represent tissue-specific stem or progenitor cells. However, no studies have addressed the issue of whether...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0641-9
更新日期：2002-12-01 00:00:00
abstract：:The opioid peptide dynorphin A (1-17) is the third transmitter identified in the striatonigral projection, the other two being gamma-aminobutyric acid (GABA) and substance P. The ultrastructural features of the dynorphinergic terminals in substantia nigra/pars reticulata were studied using pre-embedding immunocytochem...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00329443
更新日期：1990-07-01 00:00:00
