Co-axial wet-spinning in 3D bioprinting: state of the art and future perspective of microfluidic integration.

abstract：:Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several p...

journal_title：Biofabrication

authors： Seidel J,Ahlfeld T,Adolph M,Kümmritz S,Steingroewer J,Krujatz F,Bley T,Gelinsky M,Lode A

更新日期：2017-11-14 00:00:00

abstract：:Cell cultures for tissue engineering are traditionally prepared on two-dimensional or three-dimensional scaffolds with simple pores; however, this limits mass transportation, which is necessary for cell viability and function. In this paper, an innovative method is proposed for fabricating porous scaffolds with design...

journal_title：Biofabrication

authors： Liu Y,Li X,Qu X,Zhu L,He J,Zhao Q,Wu W,Li D

更新日期：2012-03-01 00:00:00

abstract：:A critical consideration in tissue engineering is to recapitulate the microstructural organization of native tissues that is essential to their function. Scaffold-based techniques have focused on achieving this via the contact guidance principle wherein topographical cues offered by scaffold fibers direct migration an...

journal_title：Biofabrication

authors： Chansoria P,Narayanan LK,Schuchard K,Shirwaiker R

更新日期：2019-04-26 00:00:00

abstract：:Here we developed a composite scaffold of pearl/poly(lactic-co-glycolic acid) (pearl/PLGA) utilizing the low-temperature deposition manufacturing (LDM). LDM makes it possible to fabricate scaffolds with designed microstructure and macrostructure, while keeping the bioactivity of biomaterials by working at a low temper...

journal_title：Biofabrication

authors： Xu M,Li Y,Suo H,Yan Y,Liu L,Wang Q,Ge Y,Xu Y

更新日期：2010-06-01 00:00:00

abstract：:Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this re...

journal_title：Biofabrication

authors： Bosch Rué E,Delgado LM,Gil FJ,Perez RA

更新日期：2020-09-30 00:00:00

abstract：:In conventional primary cultures, cardiac cells prepared from a newborn rat undergo spontaneous formation of cell clusters after several days. These cell clusters may be non-homogeneously distributed on a flat surface and show irregular beating which can be recorded by calcium ion imaging. In order to improve the cell...

journal_title：Biofabrication

authors： Wang L,Liu L,Magome N,Agladze K,Chen Y

更新日期：2013-09-01 00:00:00

abstract：:Bioprinting of living cells is rapidly developing as an advanced biofabrication approach to engineer tissues. Bioinks can be extruded in three-dimensions (3D) to fabricate complex and hierarchical constructs for implantation. However, a lack of functionality can often be attributed to poor bioink properties. Indeed, a...

journal_title：Biofabrication

authors： Cidonio G,Alcala-Orozco CR,Lim KS,Glinka M,Mutreja I,Kim YH,Dawson JI,Woodfield TBF,Oreffo ROC

更新日期：2019-06-12 00:00:00

abstract：:Electrospinning has been widely used to produce ultrafine fibers in microscale and nanoscale; however, traditional electrospinning processes are currently beset by troublesome limitations in fabrication of 3D periodic porous structures because of the chaotic nature of the electrospinning jet. Here we report a novel st...

journal_title：Biofabrication

authors： Yuan H,Zhou Q,Li B,Bao M,Lou X,Zhang Y

更新日期：2015-11-05 00:00:00

abstract：:In this study, we report the step-gradient nanocomposite (NC) hydrogel generated easily by spatial connection of different nanocomposite hydrogel pastes varying in the concentrations of nanomaterials with the aid of a 3D printing technique. The prepared 3D printed gradient NC hydrogel has self-adhesive properties and ...

journal_title：Biofabrication

authors： Motealleh A,Çelebi-Saltik B,Ermis N,Nowak S,Khademhosseini A,Kehr NS

更新日期：2019-08-22 00:00:00

abstract：:The inkjet ejection technology used in printers has been adopted and research has been conducted on manufacturing artificial tissue by patterning cells through micronozzle ejection of small droplets containing multiple cells. However, stable injection of cells has proven difficult, owing to the frequent occurrence of ...

journal_title：Biofabrication

authors： Yamaguchi S,Ueno A,Akiyama Y,Morishima K

更新日期：2012-12-01 00:00:00

abstract：:A major challenge in muscle tissue engineering is mimicking the ordered nanostructure of native collagen fibrils in muscles. Electrospun nanofiber constructs have been proposed as promising candidate alternatives to natural extracellular matrix. Here, we introduce a novel method to fabricate a two-dimension (2D) sheet...

journal_title：Biofabrication

authors： Park SH,Koh UH,Kim M,Yang DY,Suh KY,Shin JH

更新日期：2014-06-01 00:00:00

abstract：:Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofiber meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the...

journal_title：Biofabrication

authors： Song J,Zhu G,Wang L,An G,Shi X,Wang Y

更新日期：2017-02-14 00:00:00

abstract：:The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...

journal_title：Biofabrication

authors： Cai H,Wu Z,Ao Z,Nunez A,Chen B,Jiang L,Bondesson M,Guo F

更新日期：2020-07-01 00:00:00

abstract：:This paper describes a multi-material virtual prototyping (MMVP) system for modelling and digital fabrication of discrete and functionally graded multi-material objects for biomedical applications. The MMVP system consists of a DMMVP module, an FGMVP module and a virtual reality (VR) simulation module. The DMMVP modul...

journal_title：Biofabrication

authors： Cheung HH,Choi SH

更新日期：2009-12-01 00:00:00

abstract：:Cell therapy represents a promising option for revascularization of ischemic tissues. However, injection of dispersed cells is not optimal to ensure precise homing into the recipient's vasculature. Implantation of cell-engineered scaffolds around the occluded artery may obviate these limitations. Here, we employed the...

journal_title：Biofabrication

authors： Carrabba M,De Maria C,Oikawa A,Reni C,Rodriguez-Arabaolaza I,Spencer H,Slater S,Avolio E,Dang Z,Spinetti G,Madeddu P,Vozzi G

更新日期：2016-03-24 00:00:00

abstract：:In the body, cells are surrounded by an interconnected mesh of insoluble, bioactive protein fibres to which they adhere in a well-controlled manner, embedded in a hydrogel-like highly hydrated matrix. True morphological and biochemical mimicry of this so-called extracellular matrix (ECM) remains a challenge but appear...

journal_title：Biofabrication

authors： Schulte VA,Hahn K,Dhanasingh A,Heffels KH,Groll J

更新日期：2014-06-01 00:00:00

abstract：:Tissue rings with incorporated microscaffolds have been engineered as promising building blocks for constructing biological tubes from the bottom up. However, the microscaffolds available for incorporation are very limited at present. In this paper we provide an efficient strategy to first incorporate microfluidic spu...

journal_title：Biofabrication

authors： Sun T,Shi Q,Yao Y,Sun J,Wang H,Huang Q,Fukuda T

更新日期：2019-06-25 00:00:00

abstract：:Traditional drug discovery involves the screening of lead compounds from a chemical library by using cell-based high throughput screening (HTS) procedures. This has created a demand for the development of cell-based microarray chips for HTS of compounds. Although several cell-based microarray devices and procedures fo...

journal_title：Biofabrication

authors： Fujita S,Onuki-Nagasaki R,Ikuta K,Hara Y

更新日期：2016-12-05 00:00:00

abstract：:We report on the fabrication of silicon-reinforced carbon (C:Si) structures by combinatorial pulsed laser deposition to search for the best design for a new generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbi...

journal_title：Biofabrication

authors： Mihailescu IN,Bociaga D,Popescu-Pelin G,Stan GE,Duta L,Socol G,Chifiriuc MC,Bleotu C,Lazar V,Husanu MA,Zgura I,Miculescu F,Negut I,Hapenciuc C

更新日期：2017-06-01 00:00:00

abstract：:A recently emerged approach for tissue engineering is to biofabricate tissues using cellular spheroids as building blocks. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), can be cultured to generate large numbers of cells and can presumably...

journal_title：Biofabrication

authors： Lin H,Li Q,Lei Y

更新日期：2017-04-24 00:00:00

abstract：:Current technologies for manufacturing of microfluidic devices include soft-lithography, wet and dry etching, thermoforming, micro-machining and three-dimensional (3D) printing. Among them, soft-lithography has been the mostly preferred one in medical and pharmaceutical fields due to its ability to generate polydimeth...

journal_title：Biofabrication

authors： Ozbolat V,Dey M,Ayan B,Ozbolat IT

更新日期：2019-04-16 00:00:00

abstract：:Three-dimensional (3D) printed poly(ε-caprolactone) (PCL) based scaffolds have being proposed for different tissue engineering applications. This study addresses the design and fabrication of 3D PCL constructs with different struts alignments at 90°, 45° and 90° with offset. The morphology and the mechanical behavior ...

journal_title：Biofabrication

authors： Ribeiro JFM,Oliveira SM,Alves JL,Pedro AJ,Reis RL,Fernandes EM,Mano JF

更新日期：2017-05-11 00:00:00

abstract：:Fabricating three-dimensional, biocompatible microenvironments to support functional tissue assembly remains a key challenge in cardiac tissue engineering. We hypothesized that since the omentum can be removed from patients by minimally invasive procedures, the obtained underlying matrices can be manipulated to serve ...

journal_title：Biofabrication

authors： Shevach M,Soffer-Tsur N,Fleischer S,Shapira A,Dvir T

更新日期：2014-06-01 00:00:00

abstract：:The aim of this study was to comparatively evaluate the angiogenic capacity of cocultures using either human bone marrow- or human adipose tissue-derived mesenchymal stem cells (MSCs) (BM- or AT-MSCs) with human umbilical vein endothelial cells (HUVECs) both in vitro and in vivo at early time points (i.e. days 3 and 7...

journal_title：Biofabrication

authors： Ma J,Yang F,Both SK,Prins HJ,Helder MN,Pan J,Cui FZ,Jansen JA,van den Beucken JJ

更新日期：2014-03-01 00:00:00

abstract：:Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...

journal_title：Biofabrication

authors： Soffer-Tsur N,Shevach M,Shapira A,Peer D,Dvir T

更新日期：2014-09-01 00:00:00

abstract：:Silica-bonded calcite scaffolds have been successfully 3D-printed by direct ink writing, starting from a paste comprising a silicone polymer and calcite powders, calibrated in order to match a SiO2/CaCO3 weight balance of 35/65. The scaffolds, fabricated with two slightly different geometries, were first cross-linked ...

journal_title：Biofabrication

authors： Fiocco L,Elsayed H,Badocco D,Pastore P,Bellucci D,Cannillo V,Detsch R,Boccaccini AR,Bernardo E

更新日期：2017-05-11 00:00:00

abstract：:Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve rege...

journal_title：Biofabrication

authors： Ning L,Sun H,Lelong T,Guilloteau R,Zhu N,Schreyer DJ,Chen X

更新日期：2018-06-29 00:00:00

abstract：:Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers whic...

journal_title：Biofabrication

authors： Chen S,Wu C,Liu A,Wei D,Xiao Y,Guo Z,Chen L,Zhu Y,Sun J,Luo H,Fan H

更新日期：2020-05-12 00:00:00

abstract：:Engineering surfaces with functional polymers is a crucial issue in the field of micro/nanofabrication and cell-material interface studies. For many applications of surface patterning, it does not need cells to attach on the whole surface. Herein, we introduce a novel polymer fabrication protocol of off-stoichiometry ...

journal_title：Biofabrication

authors： Liu W,Li Y,Ding X

更新日期：2017-04-24 00:00:00

abstract：:Native small intestine possesses distinct multi-scale structures (e.g., crypts, villi) not included in traditional 2D intestinal culture models for drug delivery and regenerative medicine. The known impact of structure on cell function motivates exploration of the influence of intestinal topography on the phenotype of...

journal_title：Biofabrication

authors： Koppes AN,Kamath M,Pfluger CA,Burkey DD,Dokmeci M,Wang L,Carrier RL

更新日期：2016-08-22 00:00:00