Abstract:
:Bioprinting of living cells is rapidly developing as an advanced biofabrication approach to engineer tissues. Bioinks can be extruded in three-dimensions (3D) to fabricate complex and hierarchical constructs for implantation. However, a lack of functionality can often be attributed to poor bioink properties. Indeed, advanced bioinks encapsulating living cells should: (i) present optimal rheological properties and retain 3D structure post fabrication, (ii) promote cell viability and support cell differentiation, and (iii) localise proteins of interest (e.g. vascular endothelial growth factor (VEGF)) to stimulate encapsulated cell activity and tissue ingrowth upon implantation. In this study, we present the results of the inclusion of a synthetic nanoclay, Laponite® (LPN) together with a gelatin methacryloyl (GelMA) bioink and the development of a functional cell-instructive bioink. A nanocomposite bioink displaying enhanced shape fidelity retention and interconnected porosity within extrusion-bioprinted fibres was observed. Human bone marrow stromal cell (HBMSC) viability within the nanocomposite showed no significant changes over 21 days of culture in LPN-GelMA (85.60 ± 10.27%), compared to a significant decrease in GelMA from 7 (95.88 ± 2.90%) to 21 days (55.54 ± 14.72%) (p < 0.01). HBMSCs were observed to proliferate in LPN-GelMA with a significant increase in cell number over 21 days (p < 0.0001) compared to GelMA alone. HBMSC-laden LPN-GelMA scaffolds supported osteogenic differentiation evidenced by mineralised nodule formation, including in the absence of the osteogenic drug dexamethasone. Ex vivo implantation in a chick chorioallantoic membrane model, demonstrated excellent integration of the bioink constructs in the vascular chick embryo after 7 days of incubation. VEGF-loaded LPN-GelMA constructs demonstrated significantly higher vessel penetration than GelMA-VEGF (p < 0.0001) scaffolds. Integration and vascularisation was directly related to increased drug absorption and retention by LPN-GelMA compared to LPN-free GelMA. In summary, a novel light-curable nanocomposite bioink for 3D skeletal regeneration supportive of cell growth and growth factor retention and delivery, evidenced by ex vivo vasculogenesis, was developed with potential application in hard and soft tissue reparation.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Cidonio G,Alcala-Orozco CR,Lim KS,Glinka M,Mutreja I,Kim YH,Dawson JI,Woodfield TBF,Oreffo ROCdoi
10.1088/1758-5090/ab19fdsubject
Has Abstractpub_date
2019-06-12 00:00:00pages
035027issue
3eissn
1758-5082issn
1758-5090journal_volume
11pub_type
杂志文章相关文献
Biofabrication文献大全abstract::Engineering surfaces with functional polymers is a crucial issue in the field of micro/nanofabrication and cell-material interface studies. For many applications of surface patterning, it does not need cells to attach on the whole surface. Herein, we introduce a novel polymer fabrication protocol of off-stoichiometry ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa669c
更新日期:2017-04-24 00:00:00
abstract::Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions. Selecting the best strategy for bioink deposition is often challenging...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5090/aae605
更新日期:2018-11-09 00:00:00
abstract::Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/3/035023
更新日期:2014-09-01 00:00:00
abstract::A major challenge in muscle tissue engineering is mimicking the ordered nanostructure of native collagen fibrils in muscles. Electrospun nanofiber constructs have been proposed as promising candidate alternatives to natural extracellular matrix. Here, we introduce a novel method to fabricate a two-dimension (2D) sheet...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/024107
更新日期:2014-06-01 00:00:00
abstract::Tissue rings with incorporated microscaffolds have been engineered as promising building blocks for constructing biological tubes from the bottom up. However, the microscaffolds available for incorporation are very limited at present. In this paper we provide an efficient strategy to first incorporate microfluidic spu...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab1ee5
更新日期:2019-06-25 00:00:00
abstract::The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab41b4
更新日期:2019-10-21 00:00:00
abstract::Native small intestine possesses distinct multi-scale structures (e.g., crypts, villi) not included in traditional 2D intestinal culture models for drug delivery and regenerative medicine. The known impact of structure on cell function motivates exploration of the influence of intestinal topography on the phenotype of...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/3/035011
更新日期:2016-08-22 00:00:00
abstract::The physical and mechanical properties of cells modulate their behavior such proliferation rate, migration and extracellular matrix remodeling. In order to study cell behavior in a tissue-like environment in vitro, it is of utmost importance to develop biologically and physically relevant 3D cell models. Here, we char...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa6ed5
更新日期:2017-05-11 00:00:00
abstract::The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab9582
更新日期:2020-07-01 00:00:00
abstract::Three-dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturin...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/4/045006
更新日期:2016-10-11 00:00:00
abstract::Calcium alginate hydrogels are widely used as biocompatible materials in a substantial number of biomedical applications. This paper reports on a hybrid 3D printing and electrodeposition approach for forming 3D calcium alginate hydrogels in a controllable manner. Firstly, a specific 3D hydrogel printing system is deve...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa6ed8
更新日期:2017-06-07 00:00:00
abstract::We demonstrate a simple, accurate and versatile method to manipulate Parylene C, a material widely known for its high biocompatibility, and transform it to a substrate that can effectively control the cellular microenvironment and consequently affect the morphology and function of the cells in vitro. The Parylene C sc...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/025004
更新日期:2014-06-01 00:00:00
abstract::Cell mechanical microenvironment (CMM) significantly affects cell behaviors such as spreading, migration, proliferation and differentiation. However, most studies on cell response to mechanical stimulation are based on two-dimensional (2D) planar substrates, which cannot mimic native three-dimensional (3D) CMM. Accumu...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5082/4/4/042001
更新日期:2012-12-01 00:00:00
abstract::A bio-inspired hydrogel for 3D bioprinting of soft free-standing neural tissues is presented. The novel filler-free bioinks were designed by combining natural polymers for extracellular matrix biomimicry with synthetic polymers to endow desirable rheological properties for 3D bioprinting. Crosslinking of thiolated Plu...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab02c9
更新日期:2019-02-25 00:00:00
abstract::We demonstrate a novel application of TiO2 photocatalysis for modifying the cell affinity of a scaffold surface in a cell-culture environment. An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsor...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/3/035021
更新日期:2014-09-01 00:00:00
abstract::Calcified cartilage regeneration plays an important role in successful osteochondral repair, since it provides a biological and mechanical transition from the unmineralized cartilage at the articulating surface to the underlying mineralized bone. To biomimic native calcified cartilage in engineered constructs, here we...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf44a
更新日期:2018-12-27 00:00:00
abstract::In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate bl...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/2/025009
更新日期:2015-05-28 00:00:00
abstract::Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf625
更新日期:2018-12-27 00:00:00
abstract::Electrospinning has been widely used to produce ultrafine fibers in microscale and nanoscale; however, traditional electrospinning processes are currently beset by troublesome limitations in fabrication of 3D periodic porous structures because of the chaotic nature of the electrospinning jet. Here we report a novel st...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/4/045004
更新日期:2015-11-05 00:00:00
abstract::Plasma treatment is a method to modify the physicochemical properties of biomaterials, which consequently may affect interactions with cells. Based on the rationale that physical cues on the surface of culture substrates and implants, such as surface roughness, have proven to alter cell behaviour, we used electrospinn...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/1/015006
更新日期:2013-03-01 00:00:00
abstract::Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofiber meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa5c99
更新日期:2017-02-14 00:00:00
abstract::Silica-bonded calcite scaffolds have been successfully 3D-printed by direct ink writing, starting from a paste comprising a silicone polymer and calcite powders, calibrated in order to match a SiO2/CaCO3 weight balance of 35/65. The scaffolds, fabricated with two slightly different geometries, were first cross-linked ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa6c37
更新日期:2017-05-11 00:00:00
abstract::In the body, cells are surrounded by an interconnected mesh of insoluble, bioactive protein fibres to which they adhere in a well-controlled manner, embedded in a hydrogel-like highly hydrated matrix. True morphological and biochemical mimicry of this so-called extracellular matrix (ECM) remains a challenge but appear...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/2/024106
更新日期:2014-06-01 00:00:00
abstract::A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a rando...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaa718
更新日期:2018-02-05 00:00:00
abstract::Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). This study aimed to mimic the native osteoinductive microenvironment by developing an ECM using gene-transduced cells. The LIM mineralization protein-1 (LMP-1) gene was transferred to murine pre-osteoblast cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa8dd1
更新日期:2017-11-14 00:00:00
abstract::Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this re...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abbd27
更新日期:2020-09-30 00:00:00
abstract::Degeneration of the intervertebral disc (IVD) represents a significant musculoskeletal disease burden. Tissue engineering has proposed several strategies comprising the use of biodegradable materials to prepare scaffolds that can present mechanical properties similar to those of native IVD tissues. However, this might...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/1/015008
更新日期:2015-01-21 00:00:00
abstract::Tissue engineering aims to create implantable biomaterials for the repair and regeneration of damaged tissues. In vitro tissue engineering is generally based on static culture, which limits access to nutrients and lacks mechanical signaling. Using shear stress is controversial because in some cases it can lead to cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aba412
更新日期:2020-08-10 00:00:00
abstract::Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like str...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/3/035017
更新日期:2016-09-08 00:00:00
abstract::Electrospun fibers of natural polymers are desirable for biomedical applications such as tissue engineering. Crosslinking of electrospun fibers of natural polymers is needed to prevent dissolution in water and to enhance mechanical strength. In this study, an in situ UV-crosslinking method was developed for crosslinki...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/3/035008
更新日期:2013-09-01 00:00:00