Abstract:
:Current technologies for manufacturing of microfluidic devices include soft-lithography, wet and dry etching, thermoforming, micro-machining and three-dimensional (3D) printing. Among them, soft-lithography has been the mostly preferred one in medical and pharmaceutical fields due to its ability to generate polydimethylsiloxane (PDMS) devices with resin biocompatibility, throughput and transparency for imaging. It is a multi-step process requiring the preparation of a silicon wafer pattern, which is fabricated using photolithography according to a defined mask. Photolithography is a costly, complicated and time-consuming process requiring a clean-room environment, and the technology is not readily accessible in most of the developing countries. In addition, generated patterns on photolithography-made silicon wafers do not allow building 3D intricate shapes and silicon direct bonding is thus utilized for closed fluid channels and complex 3D structures. 3D Printing of PDMS has recently gained significant interest due to its ability to define complex 3D shapes directly from user-defined designs. In this work, we investigated Carbopol as a sacrificial gel in order to create microfluidic channels in PDMS devices. Our study demonstrated that Carbopol ink possessed a shear-thinning behavior and enabled the extrusion-based printing of channel templates, which were overlaid with PDMS to create microfluidic devices upon curing of PDMS and removal of the sacrificial Carbopol ink. To demonstrate the effectiveness of the fabricated devices, channels were lined up with human umbilical vein endothelial cells (HUVECs) and human bone marrow endothelial cells (BMECs) in separate devices, where both HUVECs and BMECs demonstrated the formation of endothelium with highly aligned cells in the direction of fluid flow. Overall, we here present a highly affordable and practical approach in fabrication of PDMS devices with closed fluid channels, which have great potential in a myriad of applications from cancer treatments to infectious disease diagnostics to artificial organs.
journal_name
Biofabricationjournal_title
Biofabricationauthors
Ozbolat V,Dey M,Ayan B,Ozbolat ITdoi
10.1088/1758-5090/ab10aesubject
Has Abstractpub_date
2019-04-16 00:00:00pages
034101issue
3eissn
1758-5082issn
1758-5090journal_volume
11pub_type
杂志文章相关文献
Biofabrication文献大全abstract::Omentum-based matrices fabricated by decellularization have the potential to serve as autologous scaffolds for tissue engineering. Transplantation of such scaffolds prepared from the patient's own biomaterial may reduce the immunogenic response after transplantation. Recently we reported on the potential of the decell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/3/035023
更新日期:2014-09-01 00:00:00
abstract::The high attrition rate of neuro-pharmaceuticals as they proceed to market necessitates the development of clinically-relevant in vitro neural microphysiological systems that can be utilized during the preclinical screening phase to assess the safety and efficacy of potential compounds. Historically, proposed models h...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab41b4
更新日期:2019-10-21 00:00:00
abstract::Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). This study aimed to mimic the native osteoinductive microenvironment by developing an ECM using gene-transduced cells. The LIM mineralization protein-1 (LMP-1) gene was transferred to murine pre-osteoblast cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa8dd1
更新日期:2017-11-14 00:00:00
abstract::Tissue engineering aims to create implantable biomaterials for the repair and regeneration of damaged tissues. In vitro tissue engineering is generally based on static culture, which limits access to nutrients and lacks mechanical signaling. Using shear stress is controversial because in some cases it can lead to cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aba412
更新日期:2020-08-10 00:00:00
abstract::Despite the widespread use as platforms for various biomedical applications, engineering hydrogels to impart multifunctionality and control physical properties, while closely mimicking the native cellular microenvironment, is still a significant challenge. Herein, nanofibers consisting of hydrophilic and photocrosslin...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab5385
更新日期:2019-12-19 00:00:00
abstract::Four-dimensional (4D) bioprinting of cell-laden constructs with programmable shape-morphing structures has gained increasing attention in the field of biofabrication and tissue engineering. Currently, most of the widely used materials for 4D printing, including N-isopropylacrylamide-based polymers, are not commonly us...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab39c5
更新日期:2019-09-13 00:00:00
abstract::Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different p...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa8114
更新日期:2017-08-02 00:00:00
abstract::Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions. Selecting the best strategy for bioink deposition is often challenging...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5090/aae605
更新日期:2018-11-09 00:00:00
abstract::The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing l...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab9582
更新日期:2020-07-01 00:00:00
abstract::This paper presents a novel continuous tool-path planning methodology for hollowed scaffold fabrication in tissue engineering. A new functionally gradient porous architecture is proposed with a continuous material deposition planning scheme. A controllable variational pore size and hence the porosity have been achieve...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/3/3/034106
更新日期:2011-09-01 00:00:00
abstract::The aim of this study was to comparatively evaluate the angiogenic capacity of cocultures using either human bone marrow- or human adipose tissue-derived mesenchymal stem cells (MSCs) (BM- or AT-MSCs) with human umbilical vein endothelial cells (HUVECs) both in vitro and in vivo at early time points (i.e. days 3 and 7...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/1/015005
更新日期:2014-03-01 00:00:00
abstract::In this paper we explore the printability of reversible networks formed by catechol functionalized PEG solutions and metal cations (Al3+, Fe3+ or V3+). The printability and shape fidelity were dependent on the ink composition (metal ion type, pH, PEG molecular weight) and printing parameters (extrusion pressure and pr...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab673a
更新日期:2020-04-29 00:00:00
abstract::A critical consideration in tissue engineering is to recapitulate the microstructural organization of native tissues that is essential to their function. Scaffold-based techniques have focused on achieving this via the contact guidance principle wherein topographical cues offered by scaffold fibers direct migration an...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab15cf
更新日期:2019-04-26 00:00:00
abstract::Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/3/1/015005
更新日期:2011-03-01 00:00:00
abstract::In recent years, novel biofabrication technologies have enabled the rapid manufacture of hydrogel-cell suspensions into tissue-imitating constructs. The development of novel materials for biofabrication still remains a challenge due to a gap between contradicting requirements such as three-dimensional printability and...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/2/025011
更新日期:2016-05-20 00:00:00
abstract::Three-dimensional (3D) tissue models are invaluable tools that can closely reflect the in vivo physiological environment. However, they are usually difficult to develop, have a low throughput and are often costly; limiting their utility to most laboratories. The recent availability of inexpensive additive manufacturin...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/4/045006
更新日期:2016-10-11 00:00:00
abstract::Three-dimensional (3D) printed poly(ε-caprolactone) (PCL) based scaffolds have being proposed for different tissue engineering applications. This study addresses the design and fabrication of 3D PCL constructs with different struts alignments at 90°, 45° and 90° with offset. The morphology and the mechanical behavior ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa698e
更新日期:2017-05-11 00:00:00
abstract::Scaffold-free 3D cell cultures (e.g. pellet cultures) are widely used in medical science, including cartilage regeneration. Their drawbacks are high time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid generation, most culture systems remai...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/abe186
更新日期:2021-01-29 00:00:00
abstract::A bio-inspired hydrogel for 3D bioprinting of soft free-standing neural tissues is presented. The novel filler-free bioinks were designed by combining natural polymers for extracellular matrix biomimicry with synthetic polymers to endow desirable rheological properties for 3D bioprinting. Crosslinking of thiolated Plu...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/ab02c9
更新日期:2019-02-25 00:00:00
abstract::We demonstrate a novel application of TiO2 photocatalysis for modifying the cell affinity of a scaffold surface in a cell-culture environment. An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsor...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/6/3/035021
更新日期:2014-09-01 00:00:00
abstract::Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several p...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa8854
更新日期:2017-11-14 00:00:00
abstract::A recently emerged approach for tissue engineering is to biofabricate tissues using cellular spheroids as building blocks. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), can be cultured to generate large numbers of cells and can presumably...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aa663b
更新日期:2017-04-24 00:00:00
abstract::In clinical islet transplantation, allogeneic islets of Langerhans are transplanted into the portal vein of patients with type 1 diabetes, enabling the restoration of normoglycemia. After intra-hepatic transplantation several factors are involved in the decay in islet mass and function mainly caused by an immediate bl...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/2/025009
更新日期:2015-05-28 00:00:00
abstract::Electrospinning has been widely used to produce ultrafine fibers in microscale and nanoscale; however, traditional electrospinning processes are currently beset by troublesome limitations in fabrication of 3D periodic porous structures because of the chaotic nature of the electrospinning jet. Here we report a novel st...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/7/4/045004
更新日期:2015-11-05 00:00:00
abstract::The tumour microenvironment is heterogeneous and consists of multiple cell types, variable extracellular matrix (ECM) composition, and contains cell-defined gradients of small molecules, oxygen, nutrients and waste. Emerging in vitro cell culture systems that attempt to replicate these features often fail to incorpora...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/8/4/045008
更新日期:2016-10-18 00:00:00
abstract::As an alternative to conventional cell culture and animal testing, an organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of a microfluidic cha...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaa900
更新日期:2018-02-05 00:00:00
abstract::In conventional primary cultures, cardiac cells prepared from a newborn rat undergo spontaneous formation of cell clusters after several days. These cell clusters may be non-homogeneously distributed on a flat surface and show irregular beating which can be recorded by calcium ion imaging. In order to improve the cell...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5082/5/3/035013
更新日期:2013-09-01 00:00:00
abstract::Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system ...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf625
更新日期:2018-12-27 00:00:00
abstract::Calcified cartilage regeneration plays an important role in successful osteochondral repair, since it provides a biological and mechanical transition from the unmineralized cartilage at the articulating surface to the underlying mineralized bone. To biomimic native calcified cartilage in engineered constructs, here we...
journal_title:Biofabrication
pub_type: 杂志文章
doi:10.1088/1758-5090/aaf44a
更新日期:2018-12-27 00:00:00
abstract::We have developed cell sheet technology as a modern method for the fabrication of functional tissue-like and organ-like structures. This technology allows for a sheet of interconnected cells and cells in full contact with their natural extracellular environment to be obtained. A cell sheet can be patterned and compose...
journal_title:Biofabrication
pub_type: 杂志文章,评审
doi:10.1088/1758-5082/1/2/022002
更新日期:2009-06-01 00:00:00