Abstract:
:Human spermatozoa were incubated with gossypol acetic acid (100 micrograms/1 X 10(6) spermatozoa/ml) at 37 degrees C for 30 min. The drug treatment inhibited the spermatozoal motility significantly. Washing of the spermatozoa, after gossypol treatment, did not effect their motility. A partial revival in the motility of the spermatozoa was observed when gossypol treated spermatozoa were incubated, after washing, with motility stimulating factors, e.g. theophylline, dibutyryl-cAMP and Kallikrein. :This brief experiment asked the question whether the inhibition of sperm motility imposed by gossypol is reversible by washing away the polyphenol. Human spermatozoa were incubated in Bakers buffer, 1 million/ml at 37 degrees C. for 30 minutes in the presence of 100 mc gossypol. Gossypol was dissolved in ethanol and diluted to 0.01% with distilled water, then 0.01 ml was added to 1.0 ml spermatozoa. Controls contained 0.1 ml of 0.01% ethyl acetate. Gossypol reduced motility from a range of 66.1 to 79.4% to a range of 32.1 to 38.1% (p0.01). Washing the spermatozoa by centrifuging twice at 343 x g for 10 min. and reincubating in a buffer had no effect on motility. Addition of the sperm motility stimulating agents theophylline 10 mM, kallikrein 100 KIU or dibutyryl-caMP after washing away the gossypol restored motility to 55.1%, 58.7% and 50.0%, respectively (p0.01).
journal_name
Andrologiajournal_title
Andrologiaauthors
Kalla NR,Gadru N,Foo TWdoi
10.1111/j.1439-0272.1986.tb01797.xsubject
Has Abstractpub_date
1986-07-01 00:00:00pages
393-7issue
4eissn
0303-4569issn
1439-0272journal_volume
18pub_type
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