Abstract:
:Two monoclonal antibodies (MAbs) directed against human protamine P1 were realized. Anti-P1 specificity was assessed by western-blot and confirmed by ELISA. Monoclonal antibody 97-3 was selected. Protamine P1 was specifically demonstrated in human testis by immunoelectron microscopy, using 97-3 MAb and an indirect post-embedding immunogold technique. Our results clearly demonstrated the precise time of appearance of P1 protamine in the nuclei of human spermatids. P1 first appeared in the nucleus of step 5 spermatids and its concentration was increased in steps 6-8 spermatids, cytoplasm was not labelled.
journal_name
Andrologiajournal_title
Andrologiaauthors
Lescoat D,Blanchard Y,Lavault MT,Quernée D,Le Lannou Ddoi
10.1111/j.1439-0272.1993.tb02689.xsubject
Has Abstractpub_date
1993-03-01 00:00:00pages
93-9issue
2eissn
0303-4569issn
1439-0272journal_volume
25pub_type
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