Ultrastructural and immunocytochemical study of P1 protamine localization in human testis.

Abstract:

:Two monoclonal antibodies (MAbs) directed against human protamine P1 were realized. Anti-P1 specificity was assessed by western-blot and confirmed by ELISA. Monoclonal antibody 97-3 was selected. Protamine P1 was specifically demonstrated in human testis by immunoelectron microscopy, using 97-3 MAb and an indirect post-embedding immunogold technique. Our results clearly demonstrated the precise time of appearance of P1 protamine in the nuclei of human spermatids. P1 first appeared in the nucleus of step 5 spermatids and its concentration was increased in steps 6-8 spermatids, cytoplasm was not labelled.

journal_name

Andrologia

journal_title

Andrologia

authors

Lescoat D,Blanchard Y,Lavault MT,Quernée D,Le Lannou D

doi

10.1111/j.1439-0272.1993.tb02689.x

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

93-9

issue

2

eissn

0303-4569

issn

1439-0272

journal_volume

25

pub_type

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