Abstract:
:For patients with threatened fertility, preservation of it is a major concern. Although promising results have been obtained in animal models using testicular germ cell suspensions, in humans, it is crucial to first develop an efficient method of cryopreservation to be able to apply to transplantation. Thus, four reliable and available cryopreservation techniques in any fertility centre were tested to cryopreserve an enriched fraction of diploid germ cells isolated from human testicular biopsies. The protocols were evaluated based on cell viability, and the results showed significant differences between the four methods. The semen and tissue cryopreservation methods appeared to be inadequate for diploid germ cell suspensions, and programmed slow freezing gave significantly lower results than open pulled straw vitrification; the latter was found to be the protocol that best preserved cell viability. The vitrification of isolated human diploid germ cells is innovative and constitutes valuable information for cryopreservation in cases of transplants or in vitro maturation.
journal_name
Andrologiajournal_title
Andrologiaauthors
Sá R,Cremades N,Malheiro I,Sousa Mdoi
10.1111/j.1439-0272.2012.01290.xsubject
Has Abstractpub_date
2012-12-01 00:00:00pages
366-72issue
6eissn
0303-4569issn
1439-0272journal_volume
44pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::Over the past decades, there is an increasing number of association studies of telomere length (TL) and the risk and recurrence of prostate cancer (PCa), but the results are inconsistent. Hence, we identify the relevant studies published in English on or before 10 January 2019 conducting a literature review in the ele...
journal_title:Andrologia
pub_type: 杂志文章,meta分析
doi:10.1111/and.13304
更新日期:2019-08-01 00:00:00
abstract::The authors present the histological features of the testes in 1,609 cases of male sterility. In 485 cases (30.1%), histological changes were found, which did not warrant treatment. The histologic pattern of the testes was normal in only 39% of the cases of azoospermia; and only these patients were qualified for surge...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1978.tb03057.x
更新日期:1978-09-01 00:00:00
abstract::The objective of this study was to investigate the effect of ultra-rapid freezing (direct immersion in liquid nitrogen) on human spermatozoa in cryogenic vials (≥0.5 ml) at different concentrations of sucrose. After swim-up, the sperm suspensions (N = 58) were diluted with sperm preparation medium and divided into six...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12131
更新日期:2014-08-01 00:00:00
abstract::The aim of this study was to investigate possible ultrasound seminal vesicle (SV) changes in infertile patients with 'hypertrophic-congestive' (HCUF) or 'fibro-sclerotic' (FSUF) ultrasound form of male accessory gland infection (MAGI) after prolonged administration of tadalafil (TAD), a selective phosphodiesterase-5 i...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12027
更新日期:2013-12-01 00:00:00
abstract::The influence of the Wenchuan earthquake on semen quality of adult male survivors is unclear. We investigated the semen quality included 673 male survivors from the worse-affected counties in the earthquake between Aug 2008 and July 2013. Semen parameters including pH, volume, concentration, motility and morphology we...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12573
更新日期:2016-12-01 00:00:00
abstract::The androgen receptor (AR) is a ligand-activated transcription factor member of the nuclear receptor superfamily. The existence of alternatively spliced variants is well recognised for several members of this superfamily, most of them having functional importance. For example, several testicular oestrogen receptor var...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01333.x
更新日期:2013-06-01 00:00:00
abstract::There is a consensus on the diagnostic management of bacterial prostatitis (acute and chronic). In chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) the diagnostic approach remains unclear, because inflammatory and noninflammatory CP/CPPS might be one entity with varying findings over time. The WHO definition...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.2007.00828.x
更新日期:2008-04-01 00:00:00
abstract::We have studied the response of atrophic Leydig cells to gonadotrophin replacement in young hypophysectomized (HX) and GnRH antagonist (GnRH-ANT)-treated rats. Hypophysectomy was performed at 28 days of age. Age-matched rats were treated with GnRH-ANT from 28 to 51 days of age. From 45 to 51 days of age, animals were ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1997.tb00468.x
更新日期:1997-03-01 00:00:00
abstract::Monoclonal and polyclonal antibodies were used in indirect immunofluorescence microscopy to localize vimentin intermediate filaments in the rat seminiferous epithelium. During stages XII-V of the epithelial cycle, the Sertoli cells showed a reaction in the perinuclear area and vimentin-positive extensions, projecting ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1987.tb01920.x
更新日期:1987-11-01 00:00:00
abstract::Gynaecomastia, the enlargement of the male breast, is considered as an andrological disease. To date, a review on male breast cancer (MBC) has not been published in an andrological journal. The papers underlying this review were published from authors of different institutions: Clinical Genetics, Dermatology, Gynaecol...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.2004.00639.x
更新日期:2004-12-01 00:00:00
abstract::Protein in intact coagulum of fresh human ejaculate was extracted by 0.25 N HCl, the condition used for extraction of sperm basic proteins. About 75% of the total coagulum protein was found to be acid-extractable and therefore so-called coagulum basic proteins (CBP). The CBP were glycosylated and entirely distinguisha...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1983.tb00158.x
更新日期:1983-07-01 00:00:00
abstract::Neuropeptide Y (NPY) has been shown to induce contraction of isolated human penile erectile tissue and potentiate the response to noradrenaline. The purpose of our study was to measure in the cavernous and systemic blood of healthy male volunteers the course of NPY through different stages of sexual arousal. Whole blo...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2011.01182.x
更新日期:2012-05-01 00:00:00
abstract::To explore the aetiology of obstructive azoospermia (OA) in Chinese infertility patients, 133 OA patients was included in this study diagnosed and evaluated by one major specialist trained urological infertility. We retrospected the medical records to collect relative information. All of the patients had been underwen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12509
更新日期:2016-09-01 00:00:00
abstract::Several studies have shown that high oxidative stress levels are associated with varicocele. The GST (glutathione S-transferase) family of genes is critical in the protection of cells from oxidative stress because they utilise as substrates a wide variety of products of oxidative stress. The objective of this study wa...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01103.x
更新日期:2012-02-01 00:00:00
abstract::Men with hypertension often develop erectile dysfunction (ED). The present study aimed to examine the effects of sodium hydrosulphide (NaHS), a hydrogen (H2 S) donor, treatment on ED in nitric oxide synthase (NOS) inhibitor (L-NAME)-induced hypertensive rats. Forty adult Sprague-Dawley rats were divided into four grou...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13240
更新日期:2019-06-01 00:00:00
abstract::By means of a recently developed apparatus ("Separon") motile spermatozoa were concentrated in a small volume of culture medium (0,3 ml). Sperm concentration, progressive motility and mean velocity of 60 ejaculates were determined by a computerized videomicrography system. After "Separon" sperm concentration was reduc...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The aim of this study was to examine whether an association exists between glutathione S-transferase Mu-1 (GSTM1) gene polymorphism and idiopathic male infertility. Sixty men with primary idiopathic infertility and 60 fertile men, serving as controls, were recruited for the study. The polymorphism was analysed using p...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12306
更新日期:2015-06-01 00:00:00
abstract::D-chloramphenicol, at concentrations of 20 and 40 micrograms/ml, inhibited over 80% of the newly synthesized mitochondrial proteins expressed by incorporation of 3H-amino acid mixture. At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa. The coll...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1994.tb00815.x
更新日期:1994-11-01 00:00:00
abstract::Recent studies have revealed a high prevalence of leukocytospermia (> 1 x 10(6) white blood cells ml-1 semen) in male infertility patients in the USA and certain European countries, and have implicated white blood cells as a cause of infertility. Since leukocytospermia may often be attributed to male genital-tract inf...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1994.tb00783.x
更新日期:1994-05-01 00:00:00
abstract::Exposure of intact rats to increasing doses of oestradiol-17 beta in the presence of a constant amount of exogenous androgen caused a dose-related increase in the concentration of zinc in the lateral lobes and a decrease in the levels of ventral lobe prolactin binding. These changes occurred concomitantly with a dose-...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1986.tb01819.x
更新日期:1986-09-01 00:00:00
abstract::Tubular hyalinization was studied both light and electron microscopically in 22 cases. 8 of them were found in undescended testes and 5 cases represented possible intermediary forms with only partial hyalinization of the tubules. In these testes spermatogenic arrest, hypospermatogenesis, only Sertoli cells and areas w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1986.tb01746.x
更新日期:1986-01-01 00:00:00
abstract::A global increase in the incidence of subfertility is observed, and research suggests strong genetic influences that might restrict fertility directly or indirectly. It therefore becomes important to rule out the existence of genetic causes and counsel infertile couples before offering "Advanced Infertility Treatment ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13434
更新日期:2019-11-01 00:00:00
abstract::The scope of this study was to evaluate the accuracy, precision and specificity of the sperm concentration measurements by the Strömberg-Mika Cell Motion Analyser (SM-CMA). Our data show that the instrument generally underscores the sperm concentration and therefore the uncorrected measurements must be corrected by th...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1995.tb02096.x
更新日期:1995-01-01 00:00:00
abstract::Azoospermia factor (AZF) genes on the long arm of the human Y chromosome are involved in spermatogenesis, and microdeletions in the AZF region have been recognised to be the second major genetic cause of spermatogenetic failure resulting in male infertility. While screening for these microdeletions can avoid unnecessa...
journal_title:Andrologia
pub_type: 杂志文章,多中心研究
doi:10.1111/and.12662
更新日期:2017-06-01 00:00:00
abstract::Hyperactivity of the sympathetic nervous system may play an important role in primary premature ejaculation (PPE), and dapoxetine, a selective serotonin reuptake inhibitor, plays a role in its treatment. We evaluated the success of dapoxetine treatment in PPE patients with a penile sympathetic skin response (PSSR). Of...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13076
更新日期:2018-11-01 00:00:00
abstract::Studies assessing the efficacy of intralesional verapamil injection in the treatment of Peyronie's disease have yielded mixed results. The purpose of this meta-analysis is to systematise the existing literature on the efficacy of intralesional verapamil injection when used in the treatment of Peyronie's disease. The t...
journal_title:Andrologia
pub_type: 杂志文章,meta分析
doi:10.1111/and.13388
更新日期:2019-11-01 00:00:00
abstract::Analysis of seminal fluid samples from 20 ADS cases is reported for the first time. A significant decrease in the total volume of complete semen sample and sperm concentration was observed. Percentage of morphologically abnormal sperms was higher in samples from ADS cases than controls, though no linear correlation wa...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1985.tb01714.x
更新日期:1985-11-01 00:00:00
abstract::Multiple number of techniques and devices have been described concerning circumcision method so far. One of them is thermocautery device, and it has been widely used. Although there is controversy that the penis may be damaged due to the heat generated during the use of the device, not enough histopathological studies...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13968
更新日期:2021-01-09 00:00:00
abstract::Retraction: 'Aetiology and clinical profile of children with 46, XY differences of sex development at an Indian referral centre' by Vasundhera Chauhan, Rima Dada, Vandana Jain The above article, published online on 8 August 2016 in Wiley Online Library (http://wileyonlinelibrary.com), has been retracted by agreement b...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12663
更新日期:2017-11-01 00:00:00
abstract::Insulin-like factor 3 (INSL3) has an important role in the human reproductive system; however, its detailed function is still mysterious. We aimed to investigate the possibility of expression of RXFP2 receptor on human spermatozoa and to determine the anti-apoptotic and antioxidant mechanism derived the binding of INS...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13715
更新日期:2020-10-01 00:00:00