The relationship between ejaculated ram sperm mitochondrial protein synthesis and motility.

Abstract:

:D-chloramphenicol, at concentrations of 20 and 40 micrograms/ml, inhibited over 80% of the newly synthesized mitochondrial proteins expressed by incorporation of 3H-amino acid mixture. At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa. The collective motility measured by the multichannel Reflectospermiograph system, significantly enhanced the motility wave frequencies and amplitudes by 24-17% and 65-32%, respectively. Furthermore, the longevity of the collective motility was prolonged by 12-19%. In about 20% of the cases, when the original sperm motility was low, it was found that 40 micrograms ml-1 D-chloramphenicol has maximum stimulation effect on sperm motility in inverse fashion. Since the mitochondria are located adjacent to the motility initiation area, it can be speculated that the mitochondrial protein(s) directly inhibiting the axonemal-ATPase activity or indirectly blocking sperm metabolite, are essential for maintaining sperm motility.

journal_name

Andrologia

journal_title

Andrologia

authors

Twaina-Bechor E,Bartoov B

doi

10.1111/j.1439-0272.1994.tb00815.x

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

351-5

issue

6

eissn

0303-4569

issn

1439-0272

journal_volume

26

pub_type

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