Abstract:
:Microsurgical epididymal sperm aspiration (MESA) and testicular sperm extraction (TESE) require close cooperation between andrologists, urologists and gynaecologists. Intracytoplasmic sperm injections were established in Giessen in March 1994 and embryo transfer (ET) was performed in 342 of 375 patients (91.2%). The percentage of pregnancies and ongoing pregnancies are 35.4% per ET (32.3% per cycle) and 25.1% per ET (22.9% per cycle), respectively. Microsurgical procedures such as epididymovasostomy or vasovasostomy and cryopreservation of human semen are also established methods. The purpose of the present study was to describe the andrological work-up for patients before MESA and TESE. Experiments demonstrate that incubation of testicular tissue samples in IVF medium and treatment with 1 mg ml-1 pentoxifylline increase the number of extracted motile spermatozoa. Centrifugation of the medium results in a further concentration of sperm cells. If no motile spermatozoa can be found in the supernatant medium, they may be extracted directly from the testicular tissue samples by means of a micromanipulator.
journal_name
Andrologiajournal_title
Andrologiaauthors
Köhn FM,Schroeder-Printzen I,el Mulla KF,Jung A,Stalf T,Weidner W,Gips H,Künzel W,Schill WBsubject
Has Abstractpub_date
1996-01-01 00:00:00pages
77-81eissn
0303-4569issn
1439-0272journal_volume
28 Suppl 1pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::In 20 autoptically obtained testes from men of various ages, 250-290 testicular lobules per testicle could be recognized by preparation with a stereomicroscope. The most appropriate technique was the fixation of the testes in 10% formol for at least 14 days and a following dehydration in a series of increasing concent...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::To explore the aetiology of obstructive azoospermia (OA) in Chinese infertility patients, 133 OA patients was included in this study diagnosed and evaluated by one major specialist trained urological infertility. We retrospected the medical records to collect relative information. All of the patients had been underwen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12509
更新日期:2016-09-01 00:00:00
abstract::Monoclonal and polyclonal antibodies were used in indirect immunofluorescence microscopy to localize vimentin intermediate filaments in the rat seminiferous epithelium. During stages XII-V of the epithelial cycle, the Sertoli cells showed a reaction in the perinuclear area and vimentin-positive extensions, projecting ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1987.tb01920.x
更新日期:1987-11-01 00:00:00
abstract::The aim of the study was to examine possible impacts of paroxetine and agomelatine on the levels of some components that constitute the seminal vesicle fluid. As a second purpose, it was also aimed to examine how possible negative effects induced by paroxetine on seminal vesicle fluid components were affected by kissp...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13538
更新日期:2020-05-01 00:00:00
abstract::The aim of the study was to evaluate the sperm retrieval rate by microsurgical testicular sperm extraction (TESE) in familial idiopathic nonobstructive azoospermia (NOA). One hundred and nineteen patients with idiopathic NOA who underwent microsurgical TESE over the past 5 years were included. Patients were then divid...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12378
更新日期:2015-11-01 00:00:00
abstract::Maturation arrest (MA) of spermatogenesis is diagnosed on histology as interruption of spermatogenesis before the final stage without impairment of Sertoli or Leydig cells. It is considered a condition of irreversible or absolute infertility. Varicocele, which represents impairment in the testicular venous drainage sy...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01083.x
更新日期:2010-12-01 00:00:00
abstract::Oestrus urine was proved as a potential endocrine modulator in alleviating the toxicity induced by 3-methylcholanthrene (3-MC) in male rats. We, in this study, aimed to prove the attributing potential of toxicity alleviation to squalene, an oestrus-specific pheromone in rats. A single dose of 3-methylcholanthrene (25 ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13117
更新日期:2018-12-01 00:00:00
abstract::Several studies have shown that high oxidative stress levels are associated with varicocele. The GST (glutathione S-transferase) family of genes is critical in the protection of cells from oxidative stress because they utilise as substrates a wide variety of products of oxidative stress. The objective of this study wa...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01103.x
更新日期:2012-02-01 00:00:00
abstract::We aimed to study the correlation between leukocyte counts in semen and bacterial pathogens in seminal samples of infertile men, and to establish the minimum leukocyte count associated with significant bacteriospermia. A total of 116 patients who underwent evaluation of fertility were investigated using routine semen ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2008.00845.x
更新日期:2008-08-01 00:00:00
abstract::The aim of this study was to investigate the relationship between sulphur dioxide (SO2 ) signalling pathway and the changes in erectile function under low androgen levels. Thirty-six healthy male Sprague Dawley (SD) rats aged eight weeks were randomly divided into androgen replacement group, castration group and sham ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13167
更新日期:2019-02-01 00:00:00
abstract::Open and double-blind therapeutic studies were carried out on the effects of tamoxifen in oligozoospermia. The examinations involved 74 patients. The first group (n = 20) received tamoxifen 20 mg/day; the second group (n = 54) got tamoxifen or placebo for 3 months. In the open study, tamoxifen treatment led to signifi...
journal_title:Andrologia
pub_type: 临床试验,杂志文章
doi:10.1111/j.1439-0272.1985.tb01048.x
更新日期:1985-09-01 00:00:00
abstract::CAG trinucleotide repeats are coded for the polyglutamine tract in the N-terminal of the androgen receptor (AR) gene which varies in normal individuals from 6 to 36 residues. In this study, we inspected the impact of the CAG repeats on the spermatogenic defects by measuring the size of AR-CAG repeats length in a cohor...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13728
更新日期:2020-10-01 00:00:00
abstract::The effects of vasectomy on seminal viscosity, volume and pH have been investigated in freshly emitted semen of 21 healthy men before operation and three months later. Viscosity and the pH range were decreased significantly, but the decrease of the seminal volume was not significant. Reasons of changes have been discu...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1979.tb02170.x
更新日期:1979-03-01 00:00:00
abstract::The aim of the study was to investigate whether or not the effect of icariin on erectile function of SHR is associated with inhibition of eNOS uncoupling. Ten 12-week-old male WKY rats and 10 age-matched male SHR were evenly randomised into SHR control group, SHR+ icariin treatment group, WKY control group and WKY+ ic...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13084
更新日期:2018-11-01 00:00:00
abstract::A global increase in the incidence of subfertility is observed, and research suggests strong genetic influences that might restrict fertility directly or indirectly. It therefore becomes important to rule out the existence of genetic causes and counsel infertile couples before offering "Advanced Infertility Treatment ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13434
更新日期:2019-11-01 00:00:00
abstract::As physical exercise has been shown to negatively affect sperm morphology, this study was undertaken to assess the effect of a 3-min forced swimming protocol during 50 days, with and without administration of antioxidants [N-acetylcysteine (NAC) and trans-resveratrol], on sperm morphology in CD-1 mice. Forty-four 13-w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12443
更新日期:2016-04-01 00:00:00
abstract::In 1982, 89 men from all over the FRG participated in an investigation of sperm density and the presence of selected persistent environmental chemicals in their semen. Semen concentrations for the following chemicals were measured: lead, cadmium, hexachlorobenzene (HCBC), a-hexachlorocyclohexane (a-BHC), DDT and metab...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::The role of N-methyl-D-aspartate (NMDA) and non-NMDA receptors in the control of FSH and LH secretion was analysed in prepubertal male rats. In the first experiment 4, 8, 12, 16, 20 and 30-day-old males were decapitated 15 min after vehicle, NMDA or kainic acid (KA) administration. In the second experiment, 23-day-old...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1996.tb02776.x
更新日期:1996-05-01 00:00:00
abstract::Male rats ten days of age were divided in three groups: a) hemicastrated, b) sham operated and c) control. They were daily sacrificed between 30 and 50 days of age. Wet weight and dry weight were determined in testis, seminal vesicles and epididymis. The concentration of spermatozoa was measured in testis and caput ep...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1978.tb01309.x
更新日期:1978-01-01 00:00:00
abstract::Nonylphenol (NP) is known as an environmental pollutant that has adverse effects on the spermatogenesis process. In this review, we focus on (1999-2020) studies on the effect of this pollutant on the sperm parameters and the male reproductive system. Spermatogenesis is a process in which male spermatogonia (primary ge...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/and.13748
更新日期:2020-07-14 00:00:00
abstract::After a single injection of serum gonadotrophin (PMSG) at the dose of 15 IU/kg, i.m. into rams testosterone in the plasma of blood showed a significant rise between 4th and 7th day post-injection. At the same time (4th-7th day) the plasminogen activator activity (PAA) in seminal plasma was found to be increased, but t...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1991.tb02559.x
更新日期:1991-07-01 00:00:00
abstract::Next to clinical investigations, the evaluation of male fertility relies mainly on detailed sperm analyses, for example, cell counting, motility, cell morphology and vitality testing. The manual creation of a spermiogram is time- and material-consuming. Therefore, reliable high-throughput systems that may be substitut...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13149
更新日期:2019-02-01 00:00:00
abstract::In a prospective study, the polymorphism of oestrogen receptor β gene was investigated in nonobstructive azoospermia patients. Ninety infertile patients with nonobstructive azoospermia diagnosed after two semen analysis, 2 weeks apart and negative testicular sperm extraction during intracytoplasmic sperm injection, an...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12575
更新日期:2016-12-01 00:00:00
abstract::Human ejaculates collected for in vitro procedures show variably rapid increases in osmolality, depending on enzymatic degradation of compounds. Changes in osmolality can affect cell functions due to the energy consuming processes needed to control cell volume. The aim was to examine the effects of a hypotonic challen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13859
更新日期:2020-12-01 00:00:00
abstract::Alternations of estramustine binding protein (EMBP) concentration in rat prostate in relation to androgen status under hypo- and hyperthyroid conditions was estimated by means of radioimmunoassay (RIA). In hyperthyroid rats, EMBP concentration in dorsal as well as in lateral prostate was not significantly changed, but...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1991.tb02518.x
更新日期:1991-03-01 00:00:00
abstract::Flutamide, a pure antiandrogen, increases the levels of plasma luteinizing hormone but antagonizes the biological expression of androgen on target organs. Flutamide was administered to rats to study the effect of altered availability of hormones on the functional status of epididymis. The weights of ventral prostate, ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1992.tb02620.x
更新日期:1992-03-01 00:00:00
abstract::To study the opioid control on LH and FSH secretion in Klinefelter subjects (KS), the response of gonadotropin to an opioid antagonist, naloxone, was examined in 8 KS (age range 25-35 yrs) and in 8 age matched normal men. In 6 KS with low testosterone plasma levels, naloxone infusion were also performed after treatmen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1984.tb00382.x
更新日期:1984-09-01 00:00:00
abstract::Tubular hyalinization was studied both light and electron microscopically in 22 cases. 8 of them were found in undescended testes and 5 cases represented possible intermediary forms with only partial hyalinization of the tubules. In these testes spermatogenic arrest, hypospermatogenesis, only Sertoli cells and areas w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1986.tb01746.x
更新日期:1986-01-01 00:00:00
abstract::Aquaporin-9 (AQP-9) regulates tissue hydration by promoting transmembrane exchanges of both water and solutes, such as lactate. The latter is a key metabolite of primary spermatocytes and of maturing haploid germ cells (h-GCs). The present investigation was aimed at immunolocalising human AQP-9 in both normal and vari...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2009.01009.x
更新日期:2011-02-01 00:00:00
abstract::Sertoli-cell-only (SCO) syndrome, or germ cell aplasia, is diagnosed on testicular biopsy when germ cells are seen to be absent without histological impairment of Sertoli or Leydig cells. It is considered a situation of irreversible infertility. Recent studies have shown that varicocele, a bilateral disease, causes hy...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01047.x
更新日期:2010-10-01 00:00:00