Andrological work-up of patients undergoing microsurgical epididymal sperm aspiration or testicular sperm extraction.

Abstract:

:Microsurgical epididymal sperm aspiration (MESA) and testicular sperm extraction (TESE) require close cooperation between andrologists, urologists and gynaecologists. Intracytoplasmic sperm injections were established in Giessen in March 1994 and embryo transfer (ET) was performed in 342 of 375 patients (91.2%). The percentage of pregnancies and ongoing pregnancies are 35.4% per ET (32.3% per cycle) and 25.1% per ET (22.9% per cycle), respectively. Microsurgical procedures such as epididymovasostomy or vasovasostomy and cryopreservation of human semen are also established methods. The purpose of the present study was to describe the andrological work-up for patients before MESA and TESE. Experiments demonstrate that incubation of testicular tissue samples in IVF medium and treatment with 1 mg ml-1 pentoxifylline increase the number of extracted motile spermatozoa. Centrifugation of the medium results in a further concentration of sperm cells. If no motile spermatozoa can be found in the supernatant medium, they may be extracted directly from the testicular tissue samples by means of a micromanipulator.

journal_name

Andrologia

journal_title

Andrologia

authors

Köhn FM,Schroeder-Printzen I,el Mulla KF,Jung A,Stalf T,Weidner W,Gips H,Künzel W,Schill WB

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

77-81

eissn

0303-4569

issn

1439-0272

journal_volume

28 Suppl 1

pub_type

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