Separation of interstitial and tubular cells of human testis by means of an anti-substance P-antiserum. Androstane-3 beta, 17 beta-diol is exclusively formed by seminiferous tubules.

Abstract:

:Leydig cells and seminiferous tubules of human testicular tissue were successfully separated by means of an anti-substance P-antiserum. On incubation with (14C)-testosterone or (14C)-dihydrotestosterone (DHT) it could be shown that besides DHT and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) also 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) originated in the tubular compartment. The ratio 3 alpha/3 beta-diol was found to be higher (0.7) here compared to incubation with whole testicular tissue (0.2), indicating that the testicular interstitium influences the metabolism of DHT by the seminiferous tubules.

journal_name

Andrologia

journal_title

Andrologia

authors

Tamm J,Volkwein U,Schulze W

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

80-4

issue

1

eissn

0303-4569

issn

1439-0272

journal_volume

22

pub_type

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