A new method for evaluation of the acrosome reaction in viable human spermatozoa.

Abstract:

:The acrosome reaction of human spermatozoa was induced by changes of temperature. Spermatozoa were collected from fertile donors and a patient group, and selected by the "swim-up" method. The spermatozoa were treated in two different ways: Protocol I: 24 hours at room temperature followed by additional incubation at 37 degrees C for 3 hours (control), and protocol II: 24 hours at 4 degrees C followed by additional incubation at 37 degrees C for 3 hours. The acrosome reaction of the viable spermatozoa was evaluated by a new method utilizing indirect immunofluorescence with anti-outer acrosomal membrane antibodies and exposure to a hypo-osmotic medium. In fertile donors as well as in the patient group, significant induction of the acrosome reaction (20%) was evident after exposure to low temperature (4 degrees C). The spontaneous rate of acrosome reaction in the control group was below 7%.

journal_name

Andrologia

journal_title

Andrologia

authors

Sanchez R,Toepfer-Petersen E,Aitken RJ,Schill WB

doi

10.1111/j.1439-0272.1991.tb02537.x

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

197-203

issue

3

eissn

0303-4569

issn

1439-0272

journal_volume

23

pub_type

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