Immunosuppression by human seminal plasma fractionated by DEAE Sephadex A-50 ion exchange chromatography.

Abstract:

:We fractionated the whole human seminal plasma on DEAE Sephadex A-50 ion exchange columns. Complete separation was achieved in seven peaks using different salt concentrations in phosphate buffer pH 6. The seminal plasma proteins were separated by sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Human seminal plasma (SP) and its fractions were used in mixed lymphocyte reaction in vitro. Fractions 3, 4, and 7 were found to suppress the proliferation of human peripheral blood mononuclear cells to phytohemagglutinin and pokweed mitogen at a concentration of 10 micrograms ml-1 while stimulatory effect was observed at lower concentrations (1 microgram and 2.5 micrograms ml-1). Whole human SP and other fractions failed to suppress the proliferation of lymphocytes in vitro. Furthermore, the effect of human SP and its fractions was also investigated on phagocytic function of polymorphonuclear leukocytes (PMNs) using luminol dependent chemiluminescence assay stimulated with phorbol myristate acetate and opsonized yeast. Fractionated SP was found to have a suppressive effect on the luminol-dependent chemiluminescence of PMNs in the whole blood.

journal_name

Andrologia

journal_title

Andrologia

authors

Haq A,al-Tufail M,Sheth K,Abdullatif M,Hamilton C,al-Abdul Jabbar F,al-Sedairy S

doi

10.1111/j.1439-0272.1992.tb02616.x

keywords:

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

87-93

issue

2

eissn

0303-4569

issn

1439-0272

journal_volume

24

pub_type

杂志文章
  • Biological action of estrogen on the epididymis of prepubertal rhesus monkey.

    abstract::An estrogen binding protein has been demonstrated in the cytosol of the epididymis and caudal lobe of prostate of immature rhesus monkey. Scatchard plot analyses were linear, suggesting a single class of high affinity binding sites for the synthetic estrogen, R-2858 (Moxestrol) with a Kd of 5.88 X 10(-10) mol/l and a ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1985.tb01016.x

    authors: Kamal N,Agarwal AK,Jehan Q,Setty BS

    更新日期:1985-07-01 00:00:00

  • Study on correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation.

    abstract::This investigation was carried out to study the correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation. Semen samples were evaluated for sperm parameters (mass motility [MM], concentration [CON], progressive motility [PM], viability [...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12970

    authors: Lone SA,Prasad JK,Ghosh SK,Das GK,Balamurugan B,Verma MR

    更新日期:2018-02-11 00:00:00

  • Update on the impact of Chlamydia trachomatis infection on male fertility.

    abstract::With approximately 90 million cases annually, infection with Chlamydia trachomatis is the most prevalent sexually transmitted bacterial disease in the world. Considering that these infections are often asymptomatic and cause major complications like acute pelvic inflammatory disease, ectopic pregnancy, infertility or ...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1046/j.0303-4569.2003.00594.x

    authors: Gonzales GF,Muñoz G,Sánchez R,Henkel R,Gallegos-Avila G,Díaz-Gutierrez O,Vigil P,Vásquez F,Kortebani G,Mazzolli A,Bustos-Obregón E

    更新日期:2004-02-01 00:00:00

  • Evaluation of reference values of standard semen parameters in fertile Egyptian men.

    abstract::The reference values of human semen, published in the WHO's latest edition in 2010, were lower than those previously reported. The objective of this study was to evaluate reference values of standard semen parameters in fertile Egyptian men. This cross-sectional study included 240 fertile men. Men were considered fert...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12942

    authors: Zedan H,Ismail S,Gomaa A,Saleh R,Henkel R,Agarwal A

    更新日期:2018-01-15 00:00:00

  • Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats.

    abstract::The aim of the present study was to investigate the ameliorative effect of curcumin (CMN) against acute cadmium chloride (CdCl(2)) toxicity on male reproductive system in rats. CdCl(2) is known to be a heavy metal and potential environmental pollutant. For this purpose, 28 rats were equally divided into four groups; t...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2012.01273.x

    authors: Oguzturk H,Ciftci O,Aydin M,Timurkaan N,Beytur A,Yilmaz F

    更新日期:2012-08-01 00:00:00

  • Three cases of low chiasma frequency associated with infertility in man.

    abstract::Three new cases of low chiasma frequency in infertile men are described. In the first case, all cells showed abnormal diakineses; in the second, 20% of the diakineses were desynaptic, while the remaining 80% were normal; in the third, all diakineses were desynaptic and showed chromosome fragmentation. The possible mec...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1976.tb01657.x

    authors: Templado C,Marina S,Egozcue J

    更新日期:1976-01-01 00:00:00

  • Chloroquine excretion in semen following antimalarial-drug administration.

    abstract::The study described here was carried out to establish whether or not chloroquine was excreted in semen. Chloroquine was assayed in the semen of eight male volunteers (aged between 19 and 45 years) by a fluorimetric method before and after taking a total dose of 1.500 mg chloroquine base corresponding to the accepted d...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1994.tb00782.x

    authors: Adeeko AO,Dada OA

    更新日期:1994-05-01 00:00:00

  • Glutathione S-transferase Mu-1 gene polymorphism in Egyptian patients with idiopathic male infertility.

    abstract::The aim of this study was to examine whether an association exists between glutathione S-transferase Mu-1 (GSTM1) gene polymorphism and idiopathic male infertility. Sixty men with primary idiopathic infertility and 60 fertile men, serving as controls, were recruited for the study. The polymorphism was analysed using p...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12306

    authors: Roshdy OH,Hussein TM,Zakaria NH,Sabry AA

    更新日期:2015-06-01 00:00:00

  • The antiapoptotic and antioxidant effects of eugenol against cisplatin-induced testicular damage in the experimental model.

    abstract::Testicular dysfunction or damage is among the critical side effects of chemotherapeutic drugs like cisplatin. This study was mapped out to assess the possible therapeutic effect of eugenol on cisplatin-induced testicular damage. In this experimental study, a single dose of cisplatin (15 mg/kg) was given intraperitonea...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13353

    authors: Ekinci Akdemir FN,Yildirim S,Kandemir FM,Aksu EH,Guler MC,Kiziltunc Ozmen H,Kucukler S,Eser G

    更新日期:2019-10-01 00:00:00

  • Separation of interstitial and tubular cells of human testis by means of an anti-substance P-antiserum. Androstane-3 beta, 17 beta-diol is exclusively formed by seminiferous tubules.

    abstract::Leydig cells and seminiferous tubules of human testicular tissue were successfully separated by means of an anti-substance P-antiserum. On incubation with (14C)-testosterone or (14C)-dihydrotestosterone (DHT) it could be shown that besides DHT and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) also 5 alpha-an...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Tamm J,Volkwein U,Schulze W

    更新日期:1990-01-01 00:00:00

  • Effects of a DNA-specific fluorochrome, Hoechst 33258, on mouse sperm motility and fertilizing capacity.

    abstract::DNA specific fluorochrome (Hoechst 33342 and 33258) as non-toxic stains, have been widely used to measure cell density and proliferation, detect sperm-egg fusion, and observe the development of pre-implantation embryos. It has been reported that Hoechst 33342 at a concentration of 10 micrograms ml-1 had significant in...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1992.tb02632.x

    authors: Yelian FD,Dukelow WR

    更新日期:1992-05-01 00:00:00

  • The current perspective on genetic and epigenetic factors in sperm maturation in the epididymis.

    abstract::Male infertility affects approximately 30% of infertile couples. As spermatozoa mature in the epididymal lumen, their potential for mobility increases, and their protein, lipid and small RNA (sRNA) content changes, whereas capacitation and fertilisation take place in the female reproductive tract. Both of the latter p...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13989

    authors: Ozkocer SE,Konac E

    更新日期:2021-01-25 00:00:00

  • Sperm selection strategies and their impact on assisted reproductive technology outcomes.

    abstract::The application of assisted reproductive technologies (ART) has revolutionised the treatment of human infertility, giving hope to the patients previously considered incapable of establishing pregnancy. While semen analysis is performed to access whether a sample has an adequate number of viable, motile and morphologic...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13725

    authors: Pinto S,Carrageta DF,Alves MG,Rocha A,Agarwal A,Barros A,Oliveira PF

    更新日期:2020-06-28 00:00:00

  • Relationship of interleukin-6 with semen characteristics and oxidative stress in vasectomy reversal patients.

    abstract::This prospective study was performed to evaluate the relationship between interleukin-6 (IL-6), oxidative stress and sperm function following vasectomy reversal. We included 22 patients who underwent vasectomy reversal and 15 healthy sperm donors (controls) with normal sperm morphology criteria. Levels of IL-6 in the ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2005.00668.x

    authors: Nandipati KC,Pasqualotto FF,Thomas AJ Jr,Agarwal A

    更新日期:2005-08-01 00:00:00

  • Recovery from azoospermia caused by a testicular injury: a case report.

    abstract::Some cases of testicular trauma cause infertility especially when the injured testes are not removed. However, only a few long-term follow-up studies investigating endocrinological and semen parameters in patients who had testicular trauma have been conducted. Herein, we report an interesting case of a patient who spo...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12089

    authors: Hagiuda J,Ishikawa H,Hanawa Y,Marumo K

    更新日期:2014-05-01 00:00:00

  • A new approach for cryoprotectant-free freezing of goat epididymal spermatozoa.

    abstract::A cryoprotectant-free method was successfully used for rapid freezing of goat epididymal spermatozoa. Lowering sperm volume may increase the temperature exchange rate and improve the freezing output of spermatozoa. The aim of this study was to compare two different packaging types [0.25 ml French straws (FS) and 96-we...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12384

    authors: Katanbafzadeh H,Barati F,Tabandeh M

    更新日期:2015-12-01 00:00:00

  • Cryotherapy alleviates symptoms in chronic prostatitis/chronic pelvic pain syndrome: The first results.

    abstract::Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is an intractable disease. This study aimed to assess the efficacy of cryotherapy in the therapy of CP/CPPS. One hundred and seventy-two patients with CP/CPPS were randomised to receive cryotherapy or sham cryotherapy. The follow-up assessments were done at we...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13920

    authors: Peng X,Gao H,Wang J

    更新日期:2020-12-24 00:00:00

  • Personality, coping and sperm count.

    abstract::Previous research has suggested an association between personality traits and coping, as well as between coping and sperm concentration. In the present study, both domains of research were combined, leading to the formulation of specific hypotheses. A total of 54 healthy volunteers were given questionnaires twice to a...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2004.00647.x

    authors: Pook M,Tuschen-Caffier B,Kubek J,Schill WB,Krause W

    更新日期:2005-02-01 00:00:00

  • Retracted: Aetiology and clinical profile of children with 46, XY differences of sex development at an Indian referral centre.

    abstract::Retraction: 'Aetiology and clinical profile of children with 46, XY differences of sex development at an Indian referral centre' by Vasundhera Chauhan, Rima Dada, Vandana Jain The above article, published online on 8 August 2016 in Wiley Online Library (http://wileyonlinelibrary.com), has been retracted by agreement b...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12663

    authors: Chauhan V,Dada R,Jain V

    更新日期:2017-11-01 00:00:00

  • Seminal oxidative stress in patients with chronic prostatitis.

    abstract::Prostatitis has been associated with abnormal semen parameters and may be the cause of infertility in some patients. Sperm antibodies and impaired sperm motility have been observed in the semen of patients with prostatitis. More recently, seminal oxidative stress has been detected in patients with prostatitis or acces...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:

    authors: Potts JM,Pasqualotto FF

    更新日期:2003-10-01 00:00:00

  • Relationship of thyroid to estramustine binding protein (EMBP) concentration in rat prostate.

    abstract::Alternations of estramustine binding protein (EMBP) concentration in rat prostate in relation to androgen status under hypo- and hyperthyroid conditions was estimated by means of radioimmunoassay (RIA). In hyperthyroid rats, EMBP concentration in dorsal as well as in lateral prostate was not significantly changed, but...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1991.tb02518.x

    authors: Shiina H,Sumi H,Ishibe T,Usui T

    更新日期:1991-03-01 00:00:00

  • Development of primordial germ cells in the mouse.

    abstract::Primordial germ cells in the mouse are known to be derived from the epiblast. They can be identified histochemically, by their high alkaline phosphatase activity. At 8 d post coitum they have been observed within the embryonic part of the egg cylinder, at the posterior end of the primitive streak. Earlier, at 7.25 day...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0272.1992.tb02647.x

    authors: McLaren A

    更新日期:1992-09-01 00:00:00

  • Novel predictive risk factor for Erectile Dysfunction: Serum folic acid.

    abstract::The purpose of this study was to compare the serum Folic Acid (FA) levels in patients with Erectile Dysfunction (ED) and healthy controls and whether levels vary with its severity. The study was carried out on 77 sexually active individuals, out of which 41 complained of ED and 36 were apparently normal. Patients were...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13890

    authors: Gupta A,Priyadarshi S,Vyas N,Sharma G,Swain PK

    更新日期:2021-02-01 00:00:00

  • [Motivation and compliance of andrology patents].

    abstract::150 randomized patients from the andrology department of the University Hospital for Gynecology and Obstetrics, Graz, Austria have been evaluated concerning their motivation, cooperation and compliance. To gain basic data from andrology patients and to analyze consequences after the first clinical examination was one ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Pusch HH

    更新日期:1985-03-01 00:00:00

  • Decreased polyunsaturated and increased saturated fatty acid concentration in spermatozoa from asthenozoospermic males as compared with normozoospermic males.

    abstract::The lipid composition of the sperm membrane has been shown to exert a significant effect upon the functional quality of spermatozoa. We have studied fatty acid composition of the phospholipids in spermatozoa in asthenozoospermic and normozoospermic men and determined the ratio of polyunsaturated fatty acids (PUFAs) to...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2006.00735.x

    authors: Tavilani H,Doosti M,Abdi K,Vaisiraygani A,Joshaghani HR

    更新日期:2006-10-01 00:00:00

  • Association of IgA secretory component sialylation with leucocytospermia of infertile men - a pilot study.

    abstract::The aim of our pilot study was to check whether the differences in IgA secretory component (SC) sialylation are associated with leucocytospermia. In normozoospermic and leucocytospermic seminal plasmas, 78-kDa and 63-kDa SC immunoreactive bands were observed. The SC sialylation was analysed by lectin blotting, using s...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12213

    authors: Kratz EM,Ferens-Sieczkowska M

    更新日期:2014-12-01 00:00:00

  • Effect of hypothyroidism on the testes in mature rats and treatment with levothyroxine and zinc.

    abstract::This study was performed to investigate the effect of propylthiouracil (PTU) on rat testis, and to compare the results of the different treatment regimens of levothyroxine and zinc. Twenty sexually mature Wistar albino rats were subjected to PTU for 14 days intraperitoneally to make them hypothyroidic. The effect of P...

    journal_title:Andrologia

    pub_type: 杂志文章,收录出版

    doi:10.1046/j.1439-0272.2000.00324.x

    authors: Tahmaz L,Gökalp A,Kibar Y,Koçak I,Yalçin O,Ozercan Y

    更新日期:2000-03-01 00:00:00

  • Meningiomas in three male-to-female transgender subjects using oestrogens/progestogens and review of the literature.

    abstract::Sex hormones have been proposed as a possible risk factor for the development and growth of meningiomas. Hormonal therapy plays a fundamental role in the treatment of male-to-female transgenders and needs to be continued after sex reassignment surgery. Usually, this treatment leads to no adverse events; however, its i...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.12550

    authors: Ter Wengel PV,Martin E,Gooren L,Den Heijer M,Peerdeman SM

    更新日期:2016-12-01 00:00:00

  • Acute bacterial prostatitis and chronic prostatitis/chronic pelvic pain syndrome: andrological implications.

    abstract::There is a consensus on the diagnostic management of bacterial prostatitis (acute and chronic). In chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) the diagnostic approach remains unclear, because inflammatory and noninflammatory CP/CPPS might be one entity with varying findings over time. The WHO definition...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0272.2007.00828.x

    authors: Weidner W,Wagenlehner FM,Marconi M,Pilatz A,Pantke KH,Diemer T

    更新日期:2008-04-01 00:00:00

  • Nasturtium Officinale L. hydroalcoholic extract improved oxymetholone-induced oxidative injury in mouse testis and sperm parameters.

    abstract::Testicular tissue and sex hormones are sensitive to the anabolic steroids (oxymetholone/OM) due to increased free radical damage and hormonal changes. The Nasturtium officinale L. have various antioxidant compounds. The aim of the present study was to investigate N. officinale effect on OM-induced oxidative injury in ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13294

    authors: Akbari Bazm M,Khazaei M,Khazaei F,Naseri L

    更新日期:2019-08-01 00:00:00