Investigations on the motility of human spermatozoa in a defined medium in the presence of metabolic inhibitors and of carnitine.

Abstract:

:When washed human sperm were incubated in a modified Krebs-Ringer buffer solution in the absence of exogenous metabolizable substrates at 30 degrees C they maintained progressive motility for at least six hours. Under these conditions the spermatozoa apparently utilize endogenous substrates but addition of exogenous substrates (glucose, fructose, acetate, short-chain fatty acids, branched chain amino acids) did not affect the % progressive motility or % total motility of the cells. The phospholipase inhibitors, quinacrine and Upjohn No. 1002, inhibited progressive motility when added to sperm utilizing endogenous substrate, and subsequent addition of oxidative or glycolytic substrates did not reverse the inhibition. In contrast, the inhibition by KCN of progressive motility based upon utilization of endogenous substrate was reversed upon addition of glycolyzable compounds (glucose or fructose). The addition of carnitine or its acetyl-, propionyl-, isobutyryl-, valeryl- or isovaleryl esters did not consistently affect progressive or total motility of sperm samples. The inhibitor, octylsulfobetaine, inhibited sperm motility at a concentration higher than that required for inhibition of carnitine acetyltransferase or translocase activity. On the basis of these results it does not appear that exogenous carnitine has an effect on the motility of human sperm incubated under the conditions described here.

journal_name

Andrologia

journal_title

Andrologia

authors

Shalev DP,Soffer Y,Lewin LM

doi

10.1111/j.1439-0272.1986.tb01792.x

subject

Has Abstract

pub_date

1986-07-01 00:00:00

pages

368-75

issue

4

eissn

0303-4569

issn

1439-0272

journal_volume

18

pub_type

杂志文章
  • The physiology of male reproduction: Impact of drugs and their abuse on male fertility.

    abstract::Male factor accounts for about 30-50% of infertility. A common cause of male infertility is drug abuse; either illicit or prolonged use of prescribed drugs. This study provides a review of the physiology of the hypothalamic-pituitary-gonadal axis and recent literature on drugs that have been linked to male infertility...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13672

    authors: Ajayi AF,Akhigbe RE

    更新日期:2020-10-01 00:00:00

  • Treatment of infertile patients by intrauterine insemination of washed spermatozoa.

    abstract::Thirty-five couples with a variety of infertility problems were treated by a sperm-washing and intrauterine-insemination method. The husband's semen was washed with a modified Biggers, Whitten, and Whittingham's medium to obtain a seminal plasma-free, highly concentrated sperm suspension for intrauterine insemination....

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1985.tb00955.x

    authors: Wiltbank MC,Kosasa S,Rogers B

    更新日期:1985-01-01 00:00:00

  • Decline of plasma 5alpha-dihydrotestosterone (DHT) levels upon testosterone administration to elderly men with subnormal plasma testosterone and high DHT levels.

    abstract::The study was performed to measure the impact of testosterone (T) administration on circulating levels of 5alpha-dihydrotestosterone (DHT). Group 1 (32 men; mean age 61 years; mean T 6.9 +/- 1.9 nmol l(-1)) were treated for 15 months with long-acting T undecanoate. Group 2 (23 men, mean age 60 years, mean T 7.6 +/- 2....

    journal_title:Andrologia

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1439-0272.2008.00857.x

    authors: Gooren LJ,Saad F,Haide A,Yassin A

    更新日期:2008-10-01 00:00:00

  • Association between leptin, obesity, hormonal interplay and male infertility.

    abstract::Male infertility is a major health problem worldwide. We investigated a possible association between leptin, obesity, hormonal interplay and male infertility. This cross-sectional study of 313 males (178 infertile and 135 fertile) was carried out in 2017. The subjects were categorised by body mass index (BMI) and body...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13147

    authors: Amjad S,Baig M,Zahid N,Tariq S,Rehman R

    更新日期:2019-02-01 00:00:00

  • Chloroquine excretion in semen following antimalarial-drug administration.

    abstract::The study described here was carried out to establish whether or not chloroquine was excreted in semen. Chloroquine was assayed in the semen of eight male volunteers (aged between 19 and 45 years) by a fluorimetric method before and after taking a total dose of 1.500 mg chloroquine base corresponding to the accepted d...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1994.tb00782.x

    authors: Adeeko AO,Dada OA

    更新日期:1994-05-01 00:00:00

  • Isolation and identification of nucleosides/nucleotides raising testosterone and NO levels of mice serum from Chinese chive (Allium tuberosum) leaves.

    abstract::Our previous study found that Chinese chive could significantly (p < 0.01) raise testosterone and nitric oxide (NO) levels in mice serum. However, the specific functional components of this traditional remedy are still unknown. In order to isolate and identify the active constituents from Chinese chive for enhancing t...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13191

    authors: Gu S,Wang X,Zhou R,Huang J

    更新日期:2019-03-01 00:00:00

  • Effects of continuous long-term testosterone therapy (TTh) on anthropometric, endocrine and metabolic parameters for up to 10 years in 115 hypogonadal elderly men: real-life experience from an observational registry study.

    abstract::Subnormal levels of testosterone are associated with significant negative health consequences, with higher risks of all-cause and cardiovascular mortality. The numbers of studies reporting on the benefits of normalisation of testosterone is increasing but longer-term data on (elderly) men receiving testosterone treatm...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12514

    authors: Yassin AA,Nettleship J,Almehmadi Y,Salman M,Saad F

    更新日期:2016-09-01 00:00:00

  • Effect of nonylphenol on spermatogenesis: A systematic review.

    abstract::Nonylphenol (NP) is known as an environmental pollutant that has adverse effects on the spermatogenesis process. In this review, we focus on (1999-2020) studies on the effect of this pollutant on the sperm parameters and the male reproductive system. Spermatogenesis is a process in which male spermatogonia (primary ge...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13748

    authors: Malmir M,Faraji T,Ghafarizadeh AA,Khodabandelo H

    更新日期:2020-07-14 00:00:00

  • TUNEL assay-Standardized method for testing sperm DNA fragmentation.

    abstract::Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function. Although there are a number of tests available to measure SDF, the terminal de...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13738

    authors: Sharma R,Iovine C,Agarwal A,Henkel R

    更新日期:2020-07-24 00:00:00

  • [Pregnancy after vasectomy and its possible legal consequences for the surgeon].

    abstract::The necessity to check up exactly the procedure in vasectomy by the so called - Hamburg Model - is demonstrated by 4 cases. Although these patients were sterilized we could prove fertility on spermiogram, and observed pregnancy in 3 cases. Consequences in matter of law for the surgeon is pointed out. ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Kuhlwein A,Schirren C

    更新日期:1981-07-01 00:00:00

  • A study on the association between serum amyloid A and sperm concentration.

    abstract::Our aim was to compare peripheral blood and seminal fluid serum amyloid A (SAA) protein levels in men classified on the basis of sperm concentration and investigate whether SAA protein is an important marker of male infertility. A total of 74 first-attempt IVF male partners of infertile couples classified as azoosperm...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12491

    authors: Timur H,Kokanali MK,Inal HA,Tuzluoglu D,Yilmaz N

    更新日期:2016-08-01 00:00:00

  • Relationship between penile erection and the ratio of estradiol to testosterone: A retrospective study.

    abstract::Previous studies have found that the ratio of estradiol to testosterone (E2/T ratio) has a negative effect on sexual function, but the relationship between the E2/T ratio and erection of the penis is not clarified. We conducted a retrospective study of 183 patients with erectile dysfunction and 52 healthy men to inves...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13701

    authors: Pan D,Xu ZH,Gao Q,Li M,Guan Y,Zhao ST

    更新日期:2020-10-01 00:00:00

  • Assessment of preclinical effect of (+)-catechin hydrate on sexual function: An in silico and in vivo study.

    abstract::Considering dopamine-enhancing effect of (+)-catechin, the present study was designed to evaluate dopamine-2 (D2) receptor agonistic and phosphodiesterase-5 (PDE5) enzyme inhibitory effects in in silico and effect on male sexual function of Sprague Dawley rats in vivo. (+)-Catechin and standard (sildenafil and bromocr...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13737

    authors: Rai A,Gill M,Kinra M,Dsouza LA,Sumalatha S,Raj S,Shetty R,Nandakumar K,Chamallamudi MR,Kumar N

    更新日期:2020-07-02 00:00:00

  • Effect of flutamide and two novel synthetic steroids on GABA, glutamine and some oxidative stress markers in rat brain and prostate.

    abstract::Flutamide is a steroid used to treat androgen-dependent disorders and as antiepileptic, but it induces a number of non-desirable side effects. This work was aimed at assaying the effect of flutamide and two novel synthetic steroids on the levels of GABA, glutamine and oxidative stress markers. Male Wistar rats (weight...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2010.01051.x

    authors: Calderón Guzmán D,Bratoeff E,Ramírez López E,Hernández García E,Pierdant Rioja F,Osnaya Brizuela N,Trujillo Jiménez F,Barragán Mejía G,Juárez Olguín H,Santamaría Del Ángel D

    更新日期:2011-08-01 00:00:00

  • Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome.

    abstract::Various nuclear sperm alterations are reported in patients with syndromic teratozoospermia; however, this has not been clearly identified yet in total polymorphic teratozoospermia. The aim of this study was to analyse sperm aneuploidy, DNA integrity and chromatin packaging in 45 infertile patients with total polymorph...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13252

    authors: Braham A,Ghedir H,Zidi I,Sallem A,Hajlaoui A,Ajina M,Saad A,Ibala-Romdhane S

    更新日期:2019-06-01 00:00:00

  • Evaluation of various doses of testosterone on accessory reproductive organs and plasma testosterone in intact and gonadectomized rhesus monkeys (Macaca mulatta).

    abstract::The effect of 10 days i.m. treatment of testosterone propionate (TP) on plasma testosterone and accessory reproductive organs were studied in adult (6 to 6 1/2 kg) male rhesus monkeys, housed under natural light conditions using six different dose levels. The study was scheduled in the month of September and October. ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1979.tb02237.x

    authors: Mangat HK

    更新日期:1979-01-01 00:00:00

  • The effects of pirfenidone on ischaemia-reperfusion injury in testicular torsion-induced rat model.

    abstract::The aim of this study was to analyse the effect of pirfenidone against ischaemia-reperfusion injury occurring after detorsion in rats with induced testicular torsion model. Group 1 was assigned as the control group. Group 2 first had testis torsion performed, and then, testicular detorsion was performed. Group 3 had s...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13922

    authors: Kölükçü E,Firat F,Deresoy FA,Katar M,Atılgan D

    更新日期:2020-11-26 00:00:00

  • HAART and anti-Koch's impair sexual competence, sperm quality and offspring quality when used singly and in combination in male Wistar rats.

    abstract::This study investigated the impact of the administration of HAART and anti-Koch's, singly and in combination, on sexual competence and birth statistics. Adult male Wistar rats were randomised into distilled water-treated control, HAART-treated, anti-Koch's-treated and HAART + anti-Koch's-treated groups. The 56-day ora...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13951

    authors: Akhigbe RE,Hamed MA,Odetayo AF

    更新日期:2021-01-05 00:00:00

  • Robotic-assisted laparoscopic artery-sparing varicocelectomy using indocyanine green fluorescence angiography: Initial experience.

    abstract::We reported our initial experience of robotic-assisted laparoscopic artery-sparing varicocelectomy using indocyanine green (ICG) fluorescence angiography in treatment of varicocele. A total of 45 varicocelectomies in 27 patients were performed. The mean operation time was 49.1 ± 8.5 min for unilateral and 65.6 ± 8.3 m...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13774

    authors: Teng J,Jia Z,Ai X,Luo X,Guan Y,Hao X,Fei W

    更新日期:2020-12-01 00:00:00

  • Antioxidant effects of N-acetylcysteine on the male reproductive system: A systematic review.

    abstract::This study aimed to evaluate the effect of N-acetyl cysteine on the male reproductive system and consensus and classification of data found from previous studies. It is undeniable that N-acetyl cysteine as a powerful antioxidant compound can medicate many diseases such as cardiovascular, kidney, liver and reproductive...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13898

    authors: Ghafarizadeh A,Malmir M,Naderi Noreini S,Faraji T

    更新日期:2021-02-01 00:00:00

  • Purification and characterization of angiotensin converting enzyme-II from bovine seminal plasma.

    abstract::Angiotensin converting enzyme (EC 3.4.15.1) from bovine seminal plasma was shown to exist in two distinct forms. A lower molecular weight form of the enzyme having higher activity was purified to homogeneity. Final recovery of the enzyme was 18.37%. The apparent molecular weight of the enzyme was estimated to be 1.99 ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1991.tb02569.x

    authors: Sharma M,Singh US

    更新日期:1991-07-01 00:00:00

  • Soluble Fibrinogen-like protein 2 plays a role in varicocele induced male infertility.

    abstract::Varicocele is the most common cause of male infertility. Several theories have been proposed to explain how varicocele induces infertility. The role of epididymis in male infertility is not fully well established. Fibrinogen-like protein 2 is one of serine proteases and is a potent coagulant in membranous form and imm...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13626

    authors: Elesawy FM,Abdel Hafeez NA,Abdelsalam OH,Akl EM

    更新日期:2020-07-01 00:00:00

  • Effects of a DNA-specific fluorochrome, Hoechst 33258, on mouse sperm motility and fertilizing capacity.

    abstract::DNA specific fluorochrome (Hoechst 33342 and 33258) as non-toxic stains, have been widely used to measure cell density and proliferation, detect sperm-egg fusion, and observe the development of pre-implantation embryos. It has been reported that Hoechst 33342 at a concentration of 10 micrograms ml-1 had significant in...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1992.tb02632.x

    authors: Yelian FD,Dukelow WR

    更新日期:1992-05-01 00:00:00

  • Hypotonic challenge reduces human sperm motility through coiling and folding of the tail.

    abstract::Human ejaculates collected for in vitro procedures show variably rapid increases in osmolality, depending on enzymatic degradation of compounds. Changes in osmolality can affect cell functions due to the energy consuming processes needed to control cell volume. The aim was to examine the effects of a hypotonic challen...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13859

    authors: Holmes E,Björndahl L,Kvist U

    更新日期:2020-12-01 00:00:00

  • Effect of chronic chloroquine treatment on prostaglandin and human chorionic gonadotropin (hCG) stimulation of testosterone secretion by the rat testis.

    abstract::The effect of chronic chloroquine administration on prostaglandin E1 (PGE1) and human chorionic gonadotropin (hCG) stimulation of testosterone secretion by the rat Leydig cells was studied. Adult albino (Wistar strain) rats were injected with 4 mg/kg body weight of chloroquine phosphate every other day for 30 days. At...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1987.tb01900.x

    authors: Nduka EU,Dada OA

    更新日期:1987-09-01 00:00:00

  • Myocardin restores erectile function in diabetic rats: phenotypic modulation of corpus cavernosum smooth muscle cells.

    abstract::This study aimed to investigate whether gene transfer of myocardin to the penis of diabetic rats can modulate corpus cavernosum smooth muscle (CCSM) cells phenotype and restore erectile function. Five normal control rats, and 22 diabetic rats were randomly divided into four groups: rats transfected with adCMV-myocardi...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12261

    authors: He S,Zhang T,Liu Y,Liu L,Zhang H,Chen F,Wei A

    更新日期:2015-04-01 00:00:00

  • Effects of oestrogen-induced hyperprolactinemia on the rat prostate.

    abstract::Exposure of intact rats to increasing doses of oestradiol-17 beta in the presence of a constant amount of exogenous androgen caused a dose-related increase in the concentration of zinc in the lateral lobes and a decrease in the levels of ventral lobe prolactin binding. These changes occurred concomitantly with a dose-...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1986.tb01819.x

    authors: Oldereid NB,Thomassen Y,Rui H,Gordeladze JO,Haug E,Purvis K

    更新日期:1986-09-01 00:00:00

  • Effect of lycopene on testicular androgenic and anti-oxidative enzymes in cyproterone acetate-induced male infertile Wistar strain albino rats: An in vitro study.

    abstract::Incidents of male infertility are mushrooming worldwide. Oxidative stress plays a prime role for its onset. Considering this background, the study was designed to focus the direct role of lycopene on cyproterone acetate (CPA) induced testicular hypofunction in rat. Four groups have been considered including the vehicl...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13494

    authors: Tripathy A,Ara F,Ghosh P,Ghosh D

    更新日期:2020-04-01 00:00:00

  • Update on the impact of Chlamydia trachomatis infection on male fertility.

    abstract::With approximately 90 million cases annually, infection with Chlamydia trachomatis is the most prevalent sexually transmitted bacterial disease in the world. Considering that these infections are often asymptomatic and cause major complications like acute pelvic inflammatory disease, ectopic pregnancy, infertility or ...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1046/j.0303-4569.2003.00594.x

    authors: Gonzales GF,Muñoz G,Sánchez R,Henkel R,Gallegos-Avila G,Díaz-Gutierrez O,Vigil P,Vásquez F,Kortebani G,Mazzolli A,Bustos-Obregón E

    更新日期:2004-02-01 00:00:00

  • Is there a significance of histamine in the control of the human male sexual response?

    abstract::Although histamine has been suggested to be involved in the control of male sexual function, including the induction of penile erection, its role in the human corpus cavernosum penis is still poorly understood. The aim of our study was to evaluate the course of histamine plasma levels through different stages of sexua...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2011.01222.x

    authors: Uckert S,Wilken M,Stief C,Trottmann M,Kuczyk M,Becker A

    更新日期:2012-05-01 00:00:00