Abstract:
:Various nuclear sperm alterations are reported in patients with syndromic teratozoospermia; however, this has not been clearly identified yet in total polymorphic teratozoospermia. The aim of this study was to analyse sperm aneuploidy, DNA integrity and chromatin packaging in 45 infertile patients with total polymorphic teratozoospermia, and to compare obtained results with those collected from 25 fertile men. For 14 patients, the impact of nuclear sperm abnormalities on intracytoplasmic sperm injection (ICSI) outcomes was analysed. Sperm chromatin condensation was evaluated using aniline blue staining, DNA fragmentation by TUNEL assay and chromosome abnormalities by FISH. The mean DNA fragmentation index was significantly higher in patients compared to controls, weakly and positively correlated to acrosome defects (r = 0.3; p = 0.04) and positively and moderately correlated to microcephalic heads (r = 0.5; p = 0.027). The aniline blue-reacted spermatozoa rate was also high in comparison with controls, moderately and negatively correlated to progressive motility (r = -0.6; p = 0.014). Total aneuploidy rate was considerably higher in our patients. A positive and moderate correlation was found between disomy Y rate and acrosome abnormalities (r = 0.5; p = 0.048). These patients had an impaired sperm nuclear quality, which will affect the results in ICSI. Therefore, analysis of sperm chromatin condensation, DNA integrity and aneuploidy in such cases is very useful before ART.
journal_name
Andrologiajournal_title
Andrologiaauthors
Braham A,Ghedir H,Zidi I,Sallem A,Hajlaoui A,Ajina M,Saad A,Ibala-Romdhane Sdoi
10.1111/and.13252subject
Has Abstractpub_date
2019-06-01 00:00:00pages
e13252issue
5eissn
0303-4569issn
1439-0272journal_volume
51pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::Between 1994 and 2010, a total of 123 patients with obstructive azoospermia due to aplasia of vas deferens (CAVD) were surgically treated. In 110 patients, the condition was bilateral (CBAVD), 13 men had unilateral aplasia (CUAVD), and 10 patients additionally had aplasia of one kidney. All patients underwent CFTR gen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01281.x
更新日期:2012-10-01 00:00:00
abstract::A cryoprotectant-free method was successfully used for rapid freezing of goat epididymal spermatozoa. Lowering sperm volume may increase the temperature exchange rate and improve the freezing output of spermatozoa. The aim of this study was to compare two different packaging types [0.25 ml French straws (FS) and 96-we...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12384
更新日期:2015-12-01 00:00:00
abstract::This short review summarizes some recent findings on arylhydrocarbon (dioxin) receptor expression during early pregnancy in mammals. The arylhydrocarbon receptor is a ligand-activated transcription factor and was originally described as a mediator of the toxic effects of dioxins and other chlorinated hydrocarbons such...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1046/j.1439-0272.2000.00397.x
更新日期:2000-09-01 00:00:00
abstract::The pre-operative treatment of three adult male transsexuals with the anti-androgen and progestogenic compound cyproterone acetate is reported. When used in combination with cyproterone acetate, only a very small dose of oestrogen is needed to induce feminisation. The effect of this treatment on both testicular and ep...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1989.tb02447.x
更新日期:1989-09-01 00:00:00
abstract::Corpus cavernosum smooth muscle cells (CCSMCs) are important functional cells for penile erection. We evaluated the effect of transforming growth factor β1 (TGFβ1) and hepatocyte growth factor (HGF) on the viability and apoptosis of CCSMCs in vitro. CCSMCs from healthy male Sprague Dawley rats were randomly divided in...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12372
更新日期:2015-11-01 00:00:00
abstract::30 patients with normogonadotrophic oligozoospermia from 1-20 mill/ml (group A) and 30 men with a sperm density from 210-40,0 mill/ml (group B) from the andrologic laboratory of the University Clinic for Gynecology and Obstetrics, Graz, Austria were treated with a 120 mg testosteron-undecanoate per os for 100 days. On...
journal_title:Andrologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1989-01-01 00:00:00
abstract::In 1982, 89 men from all over the FRG participated in an investigation of sperm density and the presence of selected persistent environmental chemicals in their semen. Semen concentrations for the following chemicals were measured: lead, cadmium, hexachlorobenzene (HCBC), a-hexachlorocyclohexane (a-BHC), DDT and metab...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Analysis of seminal fluid samples from 20 ADS cases is reported for the first time. A significant decrease in the total volume of complete semen sample and sperm concentration was observed. Percentage of morphologically abnormal sperms was higher in samples from ADS cases than controls, though no linear correlation wa...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1985.tb01714.x
更新日期:1985-11-01 00:00:00
abstract::Nonylphenol (NP) is known as an environmental pollutant that has adverse effects on the spermatogenesis process. In this review, we focus on (1999-2020) studies on the effect of this pollutant on the sperm parameters and the male reproductive system. Spermatogenesis is a process in which male spermatogonia (primary ge...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/and.13748
更新日期:2020-07-14 00:00:00
abstract::The origin of 'natural' anti-sperm antibodies found in fertile humans, virgin girls, and boys before puberty, is quite obscure. One hypothetical mechanism relates their existence to inflammatory gastrointestinal entities: as a result of the disease, cross-reactive antibodies produced against gastrointestinal flora bin...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2007.00772.x
更新日期:2007-06-01 00:00:00
abstract::The prostate is the seat of three major causes of morbidity: benign prostatic hyperplasia, prostate cancer and prostatitis, three conditions in which inflammation has been implicated. A state of inflammation of the prostate gland, originally incited by an infection, an autoimmune response, a neurogenic stimulus or ano...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/and.13093
更新日期:2018-12-01 00:00:00
abstract::The aim of the study was to evaluate the sperm retrieval rate by microsurgical testicular sperm extraction (TESE) in familial idiopathic nonobstructive azoospermia (NOA). One hundred and nineteen patients with idiopathic NOA who underwent microsurgical TESE over the past 5 years were included. Patients were then divid...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12378
更新日期:2015-11-01 00:00:00
abstract::Epididymal sperm reserve was determined in 6 sexually mature fertile buffalo-bulls having normal process of spermatogenesis. The relative distribution of spermatozoa in the caput, corpus and cauda epididymidis averaged 5.42, 0.75 and 11.45 billion respectively. Total epididymal sperm reserve per bull averaged 36.2 bil...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1978.tb03076.x
更新日期:1978-11-01 00:00:00
abstract::The aim of this study was to analyse the impact of assistance on the comprehensibility and reliability of the Turkish version of International Index of Erectile Function (IIEF). In this study, 458 patients were asked to complete the IIEF questionnaire by themselves during their first visit and then once again during t...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01312.x
更新日期:2013-04-01 00:00:00
abstract::Open and double-blind therapeutic studies were carried out on the effects of tamoxifen in oligozoospermia. The examinations involved 74 patients. The first group (n = 20) received tamoxifen 20 mg/day; the second group (n = 54) got tamoxifen or placebo for 3 months. In the open study, tamoxifen treatment led to signifi...
journal_title:Andrologia
pub_type: 临床试验,杂志文章
doi:10.1111/j.1439-0272.1985.tb01048.x
更新日期:1985-09-01 00:00:00
abstract::In eutherian mammalian spermatozoa the capacitation is coupled to a specific type of metabolism, that is glycolysis or oxidative respiration. A cytochemical study was carried out on cytochrome oxidase and lactate dehydrogenase in human spermatozoa collected at different times during in vitro capacitation. Human sperma...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1987.tb02346.x
更新日期:1987-06-01 00:00:00
abstract::Our aim was to establish the Estonian version of the National Institutes of Health chronic prostatitis symptom index (NIH-CPSI), and to examine its validity and applicability in chronic prostatitis patients as well as in community-based study. The questionnaire was tested in 54 category III prostatitis patients. In ad...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2006.00723.x
更新日期:2006-06-01 00:00:00
abstract::The aim of this study was to investigate the underlying mechanism of androgen deficiency inducing corporal fibrosis, thereby causing erectile dysfunction (ED). Forty 12-week-old healthy male rats were divided randomly into four groups: normal control group (Control); castration group (Castration); the other 20 rats we...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12797
更新日期:2018-02-01 00:00:00
abstract::The acrosome reaction of human spermatozoa was induced by changes of temperature. Spermatozoa were collected from fertile donors and a patient group, and selected by the "swim-up" method. The spermatozoa were treated in two different ways: Protocol I: 24 hours at room temperature followed by additional incubation at 3...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1991.tb02537.x
更新日期:1991-05-01 00:00:00
abstract::MAGI is the inflammation of the accessory male glands that notoriously exerts a negative influence on male fertility. The diagnosis is integrated by clinical, laboratory and ultrasound evaluation. In particular, the ultrasound criteria were published in 1999. The aim of this study was to analyse the sensitivity and sp...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01132.x
更新日期:2012-05-01 00:00:00
abstract::We aimed to determine the protective effects of thymoquinone (TQ), against ischaemia-reperfusion (I/R) injury in the testis tissue of rats. Twenty-seven male Wistar albino rats were randomly divided into three equal groups as follows: Group I, sham group; Group II, torsion group; and Group III, torsion + thymoquinone ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12424
更新日期:2016-03-01 00:00:00
abstract::Previous studies have confirmed that spermatogenesis in homozygous Immp2l mutant male mice was normal at the age of 6 months, but was significantly abnormal at the age of 13 months. Meanwhile, oxidative stress is reported to be involved in spermatogenic impairment in old mutant mice. However, it is unclear whether ant...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12848
更新日期:2018-03-01 00:00:00
abstract::Ten subfertile men affected by idiopathic oligoteratoasthenozoospermia and exhibiting normal serum hormone levels received a long-term treatment with human pure follicle-stimulating hormone (hp-FSH) (150 IU, intramuscularly, three times per week for 6 months). Semen parameters and ultrastructural features of spermatoz...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.2000.00358.x
更新日期:2000-05-01 00:00:00
abstract::The events occurring at the maternal-foetal interface define a successful pregnancy but the current paradigm has shifted towards assessing the contribution of spermatozoa for embryogenesis. Spermatozoa with defective DNA integrity may fertilise the oocyte but affect subsequent embryonic development. The present case-c...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13171
更新日期:2019-02-01 00:00:00
abstract::Varicocele is one of the leading causes of male infertility in which oxidative stress induces DNA damages in spermatozoa of patients with varicocele. Recent studies indicated that the treatment with antioxidant agents has protective effects against the formation of reactive oxygen species (ROS). Our research aimed to ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13826
更新日期:2020-12-01 00:00:00
abstract::The hypothalamic-pituitary-testicular axis and the regulation of prolactin secretion were investigated in eleven male renal transplant recipients. Mean serum levels of testosterone and estrone were normal, whereas those of androstenedione and estradiol were low. Mean basal luteinizing hormone (LH) levels were slightly...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1988.tb00670.x
更新日期:1988-03-01 00:00:00
abstract::Alkaline (ALP) and acid (ACP) phosphomonoesterases present in the cell-free extracts of the Triton x-100 treated freshly ejaculated chicken spermatozoa were studied of some of their physico-chemical properties. Alkaline phosphomonoesterase was found to have the optimal specific activity at pH 7.6 in 100 mM Tris-HCl bu...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Hereditary angioedema is a rare disease, which is caused by deficiency of compleman c1 esterase inhibitor regulatory protein in the compleman system. Priapism is involuntary, painful and prolonged erection of penis more than 4 h without sexual desire. In this case report, we elucidated a patient diagnosed with heredit...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12297
更新日期:2015-06-01 00:00:00
abstract::The aim of this study was to evaluate the production of artefacts during preparation and observation of human sperm for ultrastructural morphology and analyse the possible reasons of causing these artefacts. Under the scanning electron microscopy, damaged sperm heads (crack or/and rupture), necks (head-neck or head-mi...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13043
更新日期:2018-09-01 00:00:00
abstract::In this randomized prospective study, we determined the conception rate following intra-uterine insemination with washed and prepared sperm, or with the first portion of a split ejaculate, in couples with longstanding male (n = 27, 70 treatment cycles) or cervical infertility (n = 14, 29 treatment cycles). Folliculoge...
journal_title:Andrologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1439-0272.1992.tb02626.x
更新日期:1992-05-01 00:00:00