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Culture of human granulosa cells from an in vitro fertilization program: effects of extracellular matrix on morphology and cyclic adenosine 3',5' monophosphate production.

Abstract：

:Human GCs obtained from patients undergoing an IVF procedure and treated with CC, hMG, and hCG, lack cAMP responsiveness to hCG stimulation in vitro. After 48 hours in culture, recovery of cAMP production was significantly higher in hCG-stimulated cells grown on dishes coated with ECM than in cells grown on uncoated dishes. This difference correlated with a higher degree of morphologic differentiation of cells cultured on ECM. It is concluded that ECM provides superior culture conditions for the recovery of hormone-stimulated cAMP production and the maintenance of morphologic differentiation of preovulatory GCs from stimulated cycles.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Furman A,Rotmensch S,Dor J,Venter A,Mashiach S,Vlodavsky I,Amsterdam A

doi

10.1016/s0015-0282(16)49596-8

subject

Has Abstract

pub_date

1986-09-01 00:00:00

pages

514-7

issue

3

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)49596-8

journal_volume

46

pub_type

杂志文章

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