Abstract:
:The properties of neuronal plasma membranes are highly specialized, since they include the receptors by which many hormones, neurotransmitters, drugs, and toxins initiate their actions, as well as the components necessary for receiving synaptic input from a variety of sources and for generating the appropriate pattern of action potentials. To be able to study plasma membranes of neurons, it is necessary to separate sufficient quantities of neurons from the other cell types, processes, and myelin found in brain. The methodology for obtaining neurons by bulk-isolation from 10-day-old rat brain has been established, and techniques for purifying the plasma membranes are presented here. The procedure involves swelling the cells in a buffered solution, homogenizing the cells vigorously, and separating the membrane fragments on discontinuous sucrose gradients. The plasma membrane fraction is enriched in plasma membrane marker enzymes and has low activity for contaminating enzymes for subcellular organelles. Electron micrographs show that the fraction consists of membrane vesicles and profiles of various sizes. The protein composition reveals over 60 proteins with molecular weights as high as 100,000 to a low of 14,000. This plasma membrane fraction now can be the focus for studying receptors, glycoproteins, and cell-specific markers of neurons in health and disease.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Poduslo SE,Chechik T,Filbin MT,Rosenfeld Jdoi
10.1002/jnr.490150412subject
Has Abstractpub_date
1986-01-01 00:00:00pages
553-67issue
4eissn
0360-4012issn
1097-4547journal_volume
15pub_type
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journal_title:Journal of neuroscience research
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pub_type: 杂志文章
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更新日期:1998-08-15 00:00:00
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更新日期:1996-03-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:1996-10-01 00:00:00
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更新日期:1991-05-01 00:00:00
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