Abstract:
:Both IgCAMs and the actin cytoskeleton play critical roles in neuronal growth cone motility and guidance. However, it is unclear how IgCAM receptors transduce signals from the plasma membrane to induce actin remodeling. Previous studies have shown that local clustering and immobilization of apCAM, the Aplysia homolog of NCAM, induces Src kinase activity and F-actin polymerization in the peripheral domain of cultured Aplysia bag cell growth cones. Therefore, we wanted to test whether the Src kinase substrate and actin regulator cortactin could be a molecular link between Src activity and actin assembly during apCAM-mediated growth cone guidance. Here, we cloned Aplysia cortactin and showed that it is abundant in the nervous system. Immunostaining of growth cones revealed a strong colocalization of cortactin with F-actin in filopodial bundles and at the leading edge of lamellipodia. Perturbation of the cytoskeleton indicated that cortactin distribution largely depends on actin filaments. Furthermore, active Src colocalized with cortactin in regions of actin assembly, including leading edge and filopodia tips. Finally, we observed that cortactin, like F-actin, localizes to apCAM adhesion sites mediating growth cone guidance. Altogether, these data suggest that cortactin is a mediator of IgCAM-triggered actin assembly involved in growth cone motility and guidance.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Decourt B,Munnamalai V,Lee AC,Sanchez L,Suter DMdoi
10.1002/jnr.21937subject
Has Abstractpub_date
2009-04-01 00:00:00pages
1057-68issue
5eissn
0360-4012issn
1097-4547journal_volume
87pub_type
杂志文章abstract::Organotypic slices are used frequently in studies of central nervous system development and function because they provide excellent experimental access with significant preservation of cellular context and relationships. Within a slice, however, a variety of factors may cause individual classes of neurons to respond d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1174
更新日期:2001-09-01 00:00:00
abstract::In the striatum, dopamine (DA) exerts a major modulatory influence on voltage- and ligand-gated currents. Previously we have shown that DA modulates glutamatergic neurotransmission and that the direction of this modulation depends on, among other factors, the glutamate and DA receptor subtypes activated. These effects...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20079
更新日期:2004-05-01 00:00:00
abstract::Deletion of the tumor suppressor gene p53 has been shown to improve the outcome in experimental models of focal cerebral ischemia and kainate-induced seizures. To evaluate the potential role of p53 in traumatic brain injury, genetically modified mice lacking a functional p53 gene (p53(-/-), n = 9) and their wild-type ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22491
更新日期:2010-11-15 00:00:00
abstract::We used a modified pial vessel disruption (PVD) protocol with adult male Wistar rats to mimic small-vessel stroke in the cerebral cortex. Within 3 weeks, this lesion develops into a single lacuna-like cavity, which is fluid-filled and encapsulated by reactive astrocytes. Minocycline treatment that commences 1 hr after...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23223
更新日期:2013-07-01 00:00:00
abstract::Photopic L-type responses were recorded from the soma and the axon terminal of horizontal cells of the catfish (Ictalurus punctatus) retina in eye-cup preparations. The responses were produced by a spot of light with 100-micron diameter (intensity, 10 microW/cm2), which was flashed or steadily illuminated and swept al...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110405
更新日期:1984-01-01 00:00:00
abstract::Recent experiments have shown that Arg, Lys, and Leu can be incorporated posttranslationally into proteins of regenerating sciatic nerves of rats. The present experiments investigate a mixture of 15 radioactive amino acids to determine if additional amino acids can be conjugated posttranslationally to proteins of rege...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250407
更新日期:1990-04-01 00:00:00
abstract::High levels of the proto-oncogene product, pp60c-src, have been found in developing and adult neural tissues as well as in certain fully mature cells of the hematopoietic lineage, e.g., platelets and myelomonocytes. Adrenal medullary chromaffin cells exhibit characteristics of both types of cells, i.e., they are deriv...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490240107
更新日期:1989-09-01 00:00:00
abstract::Abdominal muscle electromyograms were monitored in response to graded mechanical loads added to either inspiration or expiration in anesthetized cats. The surgical preparation, the apparatus, and the levels of loads applied (resistive, tracheal occlusion, and continuous positive pressure) were matched with those emplo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130413
更新日期:1985-01-01 00:00:00
abstract::The effect of two different potassium channel blockers, 4-aminopyridine (4-AP) and quinine, on the alpha 2-adrenoceptor mediated modulation of norepinephrine (NE) release was investigated. Pairs of mouse vasa deferentia were loaded with 3H-norepinephrine (3H-NE), superfused continuously, and stimulated electrically. 4...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200114
更新日期:1988-05-01 00:00:00
abstract::The regeneration of transected mouse sciatic nerves using semipermeable acrylic copolymer tubes to enclose both stumps has been qualitatively assessed from 1 to 30 weeks post-operative. Quantitative morphometric analysis of electron micrograph montages of complete transverse sections of the segment regenerated between...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490090309
更新日期:1983-01-01 00:00:00
abstract::The large, medium-sized, and small neurons of the dorsal root ganglion (DRG) have different functions in the processing of various senses. Hyperpolarization-activated, cyclic nucleotide-gated channels (HCN) contribute greatly to neuronal excitability. In the present study, which used whole-cell patch clamp techniques ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20109
更新日期:2004-06-01 00:00:00
abstract::Contacts between horizontal and bipolar cells are described in the retina of the teleost Eugerres plumieri. A single, long expansion observed in the external cone horizontal cells makes contact by means of a terminal button with the cell body of a bipolar. It represents the only contact between this class of horizonta...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490060111
更新日期:1981-01-01 00:00:00
abstract::Interleukin-1beta has been demonstrated in neurons of the rat hypothalamus, including cells of the magnocellular neurosecretory system and tuberoinfundibular system (Lechan et al., [1990] Brain Res. 514:135-140). Despite its potential importance to regulation of neuroendocrine function, however, neither the specific c...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000515)60:4<478::AID-JNR
更新日期:2000-05-15 00:00:00
abstract::Anoxia during delivery is a complication that can disturb infant brain development leading to various types of neurological disorders. Our studies have shown that increased body temperature of newborn rats of both sexes intensifies the postanoxic oxidative stress and prevents triggering the endogenous adaptive respons...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24486
更新日期:2019-10-01 00:00:00
abstract::Dissociated neurons from the neural tube containing the trigeminal motor nucleus from early chick embryos were cultured on laminin or collagen-polyornithine substrates, with and without nerve growth factor (NGF). Control cultures were grown in similar conditions with cytochrome-C. It was found that neuronal survival w...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220404
更新日期:1989-04-01 00:00:00
abstract::The C57BL/6 mice strain is known to be reputedly resistant to induction of experimental autoimmune neuritis (EAN), an animal model of Guillain-Barré syndrome in human by bovine peripheral myelin (BPM), and P2 protein or the P2 protein peptide 57-81. The P0 peptide 180-199 is a stronger neuritogenic antigen than the P2...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20001201)62:5<717::AID-JNR11>3.0
更新日期:2000-12-01 00:00:00
abstract::Green fluorescent protein (GFP) transgenic zebrafish technology has been employed to directly visualize and analyze dynamic developmental processes, such as cell migration and morphogenesis. Stable transgenic zebrafish that express GFP in oligodendrocytes can be a valuable tool to visualize complex myelination process...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20516
更新日期:2005-07-01 00:00:00
abstract::Increasing evidence suggests that the regulation of neuronal cell death is complex. In this study we compared the neurotoxic effects of tumor necrosis factor-alpha (TNFalpha), nitric oxide, and thrombin on primary rat cortical cell cultures and the neuronal PC12 cell line. Release of lactate dehydrogenase (LDH) and th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1119
更新日期:2001-06-15 00:00:00
abstract::Ionotropic N-methyl-D-aspartate (NMDA) receptor agonists, L-aspartic acid (L-Asp) and NMDA, have been shown to inhibit histamine-stimulated acid secretion, but their effect on gastric mucosal blood flow (GMBF) is largely unknown. The aim of this study was to investigate whether L-Asp and NMDA inhibit histamine-stimula...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20202
更新日期:2004-09-01 00:00:00
abstract::Apolipoprotein E (ApoE) is the major brain lipoprotein and plays an important role in lipid transport. ApoE-deficient mice whose apoE gene has been knocked out have distinct cognitive and neurochemical deficits, and their recovery from brain injury is impaired. In the present study we examined the possibility that the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-11-15 00:00:00
abstract::Direct brain injections of the N-methyl-D-aspartate receptor agonist quinolinic acid (QA) trigger an excitotoxic cascade characterized by rapid neuronal death and glial/immune cell activation. The present study compared the timing of immediate early gene (IEG; c-fos, c-jun, jun-B, and zif/268) induction with the respo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490360511
更新日期:1993-12-01 00:00:00
abstract::Myelin gene expression in normal oligodendrocytes (OLG) depends on developmentally regulated protein kinase C (PKC) enzyme activity (Asotra and Macklin: J Neurosci Res 34:571-588, 1993). We studied the developmental expression of the Ca(++)-dependent PKC-alpha, -beta 1, -beta II and -gamma isozymes, and the Ca(++)-ind...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390305
更新日期:1994-10-15 00:00:00
abstract::Neuroepithelial b2T cells were derived from the hindbrain and the spinal cord of mouse transgenic embryos, which expressed SV40 T antigen under the control of a Hoxb2 enhancer. Strikingly, b2T cell lines of either origin exhibit a very similar gene expression pattern, including markers of the hindbrain and the spinal ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10716
更新日期:2003-09-15 00:00:00
abstract::Transgenic mice with glial fibrillary acidic protein (GFAP) promoter driven-astrocyte production of the cytokines interleukin-6 (IL-6) and tumor necrosis factor (TNF) were used to determine whether the pre-existing production of these cytokines in vivo might modulate the sensitivity of neurons to excitotoxic agents. L...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10635
更新日期:2003-07-15 00:00:00
abstract::The existence of endogenous neural progenitor cells (NPCs) in the adult spinal cord (sc) provides the potential for tailored repair therapies after spinal cord injury (SCI). This study investigates the impact of inflammatory mediators on properties of NPC cultures derived from adult rats after SCI. The Infinite Horizo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23527
更新日期:2015-04-01 00:00:00
abstract::Cross-linking of alpha-synuclein and Lewy body formation have been implicated in the dopaminergic neuronal cell death observed in Parkinson's disease (PD); the mechanisms responsible, however, are not clear. Reactive oxygen species and advanced glycation end products (AGEs) have been found in the intracellular, alpha-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21644
更新日期:2008-07-01 00:00:00
abstract::The extracellular matrix surrounds different neuronal compartments in the mature nervous system. In a variety of vertebrates, most brain regions are loaded with a distinct type of extracellular matrix around the somatodendritic part of neurons, termed perineuronal nets. The present study reports that chondrotin sulfat...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22761
更新日期:2012-02-01 00:00:00
abstract::Regulatory factor X4 variant 3 (RFX4_v3) is a recently identified transcription factor specifically expressed in the brain. Gene disruption in mice demonstrated that interruption of a single allele (heterozygous, +/-) prevented formation of the subcommissural organ (SCO), resulting in congenital hydrocephalus, whereas...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.21356
更新日期:2007-12-01 00:00:00
abstract::Relationships among different intensities of electroshock treatment (EST), EEG-recordable primary after discharge (PAD), and beta-adrenergic binding in various brain regions were investigated in adult rats. Different intensities of EST (1-2, 6-8, 20-30 mamp) were applied through ear-clip electrodes for 7 consecutive d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490070306
更新日期:1982-01-01 00:00:00
abstract::The mutant hypogonadal (hpg) mouse lacks a functioning gene for the neurohormone gonadotropin releasing hormone (GnRH). Previous studies from our laboratory had indicated that the initiation and maintenance of reproductive function in these mice could be brought about by the implantation of normal fetal grafts into ad...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490310121
更新日期:1992-01-01 00:00:00