Abstract：
:We have an interest in the cellular response to mechanical stimuli, and here describe an in-vitro method to examine the response of cells cultured in a three-dimensional matrix to mechanical compressive and tensile stress. Synthetic aliphatic polyester scaffolds coated with 45S5 bioactive glass were seeded with human dental follicular cells (HDFC), and attached to well inserts and magnetic endplates in six well palates. Scaffolds were subjected to either cyclic 10% tensile deformation, or 8% compression, at 1 Hz and 2 Hz respectively for 6, 24 or 48 h, by uniaxial motion of magnetically-coupled endplates. It was possible to isolate high quality mRNA from cells in these scaffolds, as demonstrated by high RNA integrity numbers scores, and ability to perform meaningful cRNA microarray analysis, in which 669 and 727 genes were consistently upregulated, and 662 and 518 genes down regulated at all times studied under tensile and compressive loading conditions respectively. MetaCore analysis revealed the most regulated gene ontogenies under both loading conditions to be for: cytoskeletal remodelling; cell adhesion-chemokines and adhesion; cytoskeleton remodelling-TGF WNT and cytoskeletal remodelling pathways. We believe the method here described will be of value for analysis of the cellular response to cyclic loading.
journal_name
J Mech Behav Biomed Materauthors
Sarrafpour B,Boughton P,Farahani RM,Cox SC,Denyer G,Kelly E,Zoellner Hdoi
10.1016/j.jmbbm.2018.08.006subject
Has Abstractpub_date
2018-12-01 00:00:00pages
11-17eissn
1751-6161issn
1878-0180pii
S1751-6161(18)30521-6journal_volume
88pub_type
杂志文章abstract：:In the present work, we propose the first structural constitutive model of the passive mechanical behavior of the swine colon that is validated against physiological inflation-extension tests, and accounts for residual strains. Sections from the spiral colon and the descending colon were considered to investigate pote...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2017.08.031
更新日期：2018-01-01 00:00:00
abstract：:Bone fracture is a major health issue worldwide and consequently there have been extensive investigations into the fracture behavior of human cortical bone. However, the fracture properties of human cortical bone under fall-like loading conditions remains poorly documented. Further, most published research has been pe...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2017.03.015
更新日期：2017-07-01 00:00:00
abstract：:Osteosynthesis plates are clinically used to fixate and position a fractured bone. They should have the ability to withstand cyclic loads produced by muscle contractions and total body weight. The very high demand for osteosynthesis plates in developing countries in general and in Indonesia in particular necessitates ...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2012.04.018
更新日期：2012-09-01 00:00:00
abstract：:This paper presents a strain-based damage model to predict the stress-strain relationship and investigate the damage onset and evolution of the fibre and matrix of a fully bio-resorbable phosphate glass fibre reinforced composite under three-point bending. The flexural properties of the composite are crucial, particul...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2018.02.022
更新日期：2018-05-01 00:00:00
abstract：:The superior biotribological performance of nanocrystalline diamond (NCD) coatings grown by a chemical vapor deposition (CVD) method was already shown to demonstrate high wear resistance in ball on plate experiments under physiological liquid lubrication. However, tests with a close-to-real approach were missing and t...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2015.05.005
更新日期：2015-09-01 00:00:00
abstract：:The repair of abdominal wall defects often requires the use of polypropylene (PP) as the main material. After a PP mesh is implanted in the body, contact with the intestine can cause adhesions between the intestine and the mesh, leading to serious complications such as intestinal fistula. In this study, we used electr...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2020.104087
更新日期：2020-12-01 00:00:00
abstract：:Poly (glycerol sebacate) (PGS) is a promising elastomer for use in soft tissue engineering. However, it is difficult to achieve with PGS a satisfactory balance of mechanical compliance and degradation rate that meet the requirements of soft tissue engineering. In this work, we have synthesised a new PGS nanocomposite ...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2011.05.038
更新日期：2011-11-01 00:00:00
abstract：:The high-performance polymer PEEK (poly-ether-ether-ketone) is more and more being used in the field of dentistry, mainly for removable and fixed prostheses. In cases of screw-retained implant-supported reconstructions of PEEK, an abutment screw made of PEEK might be advantageous over a conventional metal screw due to...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2016.06.024
更新日期：2016-10-01 00:00:00
abstract：:The aim of the present work was twofold: to prepare biphasic magnesium fluorapatite (MFA) composites with different amounts of alumina using a two-step sintering process, and to evaluate the effects of various amounts of alumina on the mechanical properties, phase stability, and densification of the composite samples....
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2014.08.004
更新日期：2014-12-01 00:00:00
abstract：:Use of hydroxyapatite (HAP) for biomaterials is widely established, often in a combination with titanium alloy substrates in orthopaedic and other implants. Porous HAP-based coatings undergo sintering and heat treatment processes to achieve proper level of density yet avoiding undesirable reactions and phase changes. ...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2013.11.017
更新日期：2014-02-01 00:00:00
abstract：:Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications. Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to ob...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2021.104337
更新日期：2021-01-21 00:00:00
abstract：:The differentiation of hepatic stellate cells (HSCs) into myofibroblasts is a key event in liver fibrosis. Due to the local stiffening of the extracellular matrix (ECM) during fibrosis, it is of great interest to develop mimics that can be used to investigate the cellular response to changes in mechanics. Here, we use...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2013.11.008
更新日期：2014-10-01 00:00:00
abstract：:Extensive remodeling of the myocardium is seen in a variety of cardiovascular diseases, including systemic hypertension. Stem cell therapy has been proposed to improve the clinical outcomes of hypertension, and we hypothesized that changes in mechanical properties of the myocardium would accompany the progression of d...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2015.11.041
更新日期：2016-06-01 00:00:00
abstract：:The retrogradation of high amylose starch (5% or 10%), by isothermal cycles at 4°C (method 1) or by alternating thermal cycles (method 2) was efficient and promoted important structural modifications. Hydrogels of gellan gum and starch retrograded blends, containing or not ketoprofen, were prepared by ionic and dual c...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2016.08.005
更新日期：2017-01-01 00:00:00
abstract：:Laser surface melting (LSM) of Ti6Al4V alloy was carried out with an aim to improve properties such as microstructure and wear for implant applications. The alloy substrate was melted at 250W and 400W at a scan velocity of 5mm/s, with input energy of 42J/mm(2) and 68J/mm(2), respectively. The results showed that equia...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2013.12.001
更新日期：2014-04-01 00:00:00
abstract：:With increasing interest in treating osteoarthritis at its earliest stages, it has become important to understand the mechanisms by which the disease progresses across a joint. Here, second harmonic generation (SHG) microscopy, coupled with a two-dimensional spring-mass network model, was used to image and investigate...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2011.08.004
更新日期：2012-01-01 00:00:00
abstract：:Crosslinked Ultra High Molecular Weight Polyethylene (UHMWPE), which is used as a bearing material in total joint replacement components, is subjected to static and cyclic loads in vivo. Resistance to crack initiation from a notch as a function of static and cyclic loads is not well understood for crosslinked UHMWPE. ...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2018.12.032
更新日期：2019-03-01 00:00:00
abstract：:Main objective of this study is to investigate the thermal behavior of ceramic laminate veneer restorations of the maxillary central incisor with different incisal preparations such as butt joint and palatinal chamfer using finite element method. In addition, it is also aimed to understand the effect of different ther...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2017.07.039
更新日期：2017-11-01 00:00:00
abstract：:Mechanical property data for brain tissue are needed to understand the biomechanics of neurological disorders and response of the brain to different mechanical and surgical forces. Most studies have characterized mechanical behavior of brain tissues over large regions or classified tissue properties for either gray or...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2013.08.026
更新日期：2014-01-01 00:00:00
abstract：:This review attempts to show the bone community that there are many ways of being a 'bone', and that the range of mechanical properties of bone material is much greater than is conventionally thought to be the case. However the structure-function relationships have in many cases hardly moved beyond mere assertion. The...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章,评审
doi：10.1016/j.jmbbm.2010.03.002
更新日期：2010-07-01 00:00:00
abstract：:The aim of the presented study was to estimate the material properties of human patellar ligaments from the elderly population by means of tensile tests. The experimental part was conducted on a custom tensile-testing device, with a built-in enclosure to simulate in-vivo conditions, using 25 (15 female, 10 male) bone-...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2020.103994
更新日期：2020-10-01 00:00:00
abstract：:Polylactic acid (PLA), one of the most likely green and environmentally friendly materials, is an alternative to petroleum-based plastic. It still remains a challenge to increase the degradation rate and decrease the cost of PLA without compromised mechanical properties. Low cost PLA/wood flour (WF) composite was elab...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2018.08.016
更新日期：2018-12-01 00:00:00
abstract：:With the aim to prepare antibacterial acrylic bone cements, a series of imidazolium-containg mono-methacrylates with N-alkyl chain length varied from 10 to 18 (MEIM-x) were synthesized as polymerizable antibacterial agents through a two-step reaction. The structures of the monomers were confirmed by 1H NMR. Methyl met...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2017.06.003
更新日期：2017-10-01 00:00:00
abstract：:This paper presents a new approach to study the effects of temperature on the poro- elastic and viscoelastic behavior of articular cartilage. Biphasic solid-fluid mixture theory is applied to study the poro-mechanical behavior of articular cartilage in a fully saturated state. The balance of linear momentum, mass, and...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2017.11.023
更新日期：2018-02-01 00:00:00
abstract：:In this paper an analytical solution for the stress state in a coated stent is presented, with a particular focus on the interface stresses between the coating and stent. As a first step a simplified stent architecture consisting of a bi-layered composite elastic arch is considered. The variations of normal and shear ...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2012.02.009
更新日期：2012-06-01 00:00:00
abstract：:A newly designed hydroxyapatite-polyurethane (HA-PU) composite scaffold was prepared by polymerizing glyceride of castor oil (GCO) with isophorone diisocyanate (IPDI) and HA as fillers. The aim of this study was to determine the effect of HA fillers on the mechanical properties and osteogenesis capacity of the composi...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2018.08.028
更新日期：2018-12-01 00:00:00
abstract：:The current study deals with the preparation and the characterization of the PVA-CMT-GO films. The PVA-CMT film was translucent in nature and smooth to touch. The addition of GO resulted in the formation of agglomerated structures. XRD studies suggested that the incorporation of GO increased the average crystallite si...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2018.02.021
更新日期：2018-05-01 00:00:00
abstract：:Proper statistical analysis is essential in the research studies. In particular, as regards the in vitro testing of the lumbar spine, the criteria for the standardization have been extensively discussed but the use of statistics has not been reviewed. Unfortunately, cadaveric testing is a very difficult and complex ex...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2020.103862
更新日期：2020-10-01 00:00:00
abstract：:Microinjection is a widely used technique to deliver foreign materials into biological cells. We propose a mathematical model to study the mechanical behavior of a cell in microinjection. Firstly, a cell is modeled by a hyperelastic membrane and interior cytoplasm. Then, based on the fact that the equilibrium configur...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2013.04.017
更新日期：2013-08-01 00:00:00
abstract：:Porous poly(para-phenylene) (PPP) scaffolds have tremendous potential as an orthopedic biomaterial; however, the underlying mechanisms controlling the monotonic and cyclic behavior are poorly understood. The purpose of this study was to develop a method to integrate micro-computed tomography (μCT), finite-element anal...
journal_title：Journal of the mechanical behavior of biomedical materials
pub_type： 杂志文章
doi：10.1016/j.jmbbm.2014.10.004
更新日期：2015-01-01 00:00:00
