当前位置： SCI文献检索 > Journal of the Mechanical Behavior of Biomedical Materials期刊下所有文献 > Hydrogels with differential and patterned mechanics to study stiffness-mediated myofibroblastic differentiation of hepatic stellate cells.

Hydrogels with differential and patterned mechanics to study stiffness-mediated myofibroblastic differentiation of hepatic stellate cells.

Abstract：

:The differentiation of hepatic stellate cells (HSCs) into myofibroblasts is a key event in liver fibrosis. Due to the local stiffening of the extracellular matrix (ECM) during fibrosis, it is of great interest to develop mimics that can be used to investigate the cellular response to changes in mechanics. Here, we used a step-wise hydrogel crosslinking technique, where macromolecules are crosslinked using a sequence of addition then UV light-mediated radical crosslinking, to generate hydrogels with tunable stiffness. Freshly isolated HSCs remained rounded with lipid droplets and high levels of PPARγ expression on soft substrates (E~2kPa); however, HSCs spread, lost their lipid droplets, and expressed high levels of α-smooth muscle actin (α-SMA) and type I collagen on stiff substrates (E~24kPa). Similarly, fully differentiated cells reverted to a quiescent state when plated on soft substrates. Stiffness-induced differentiation of HSCs was enhanced in the presence of exogenous TGF-β1, a dominant signal in fibrosis. When the UV-induced secondary crosslinking was restricted with a photomask to spatially control mechanics, HSCs responded based on the local hydrogel stiffness, although they remained quiescent on stiff substrates if the stiff feature size was not sufficient to allow cell spreading. This hydrogel system permits the investigation of HSC response to materials with diverse levels and spatially heterogeneous mechanical properties.

journal_name

J Mech Behav Biomed Mater

journal_title

Journal of the mechanical behavior of biomedical materials

authors

Guvendiren M,Perepelyuk M,Wells RG,Burdick JA

doi

10.1016/j.jmbbm.2013.11.008

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

198-208

eissn

1751-6161

issn

1878-0180

pii

S1751-6161(13)00395-0

journal_volume

38

pub_type

杂志文章

相关文献

Journal of the Mechanical Behavior of Biomedical Materials文献大全
  • R-curve behavior of a short-fiber reinforced resin composite after water storage.

    abstract：:As a supplement to our investigation into R-curves in a short-fiber reinforced dental resin composite, we investigate the effects of 2-months water storage. Water is known to degrade the siloxane bonds linking matrix and fillers, which has been suggested to decrease the fracture toughness in conventional resin composi...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2020.103674

    authors： Tiu J,Belli R,Lohbauer U

    更新日期：2020-04-01 00:00:00

  • "Force-from-lipids" gating of mechanosensitive channels modulated by PUFAs.

    abstract：:The level of fatty acid saturation in phospholipids is a crucial determinant of the biophysical properties of the lipid bilayer. Integral membrane proteins are sensitive to changes of their bilayer environment such that their activities and localization can be profoundly affected. When incorporated into phospholipids ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2017.12.026

    authors： Ridone P,Grage SL,Patkunarajah A,Battle AR,Ulrich AS,Martinac B

    更新日期：2018-03-01 00:00:00

  • Evaluation of the mechanical properties of powder metallurgy Ti-6Al-7Nb alloy.

    abstract：:Titanium and its alloys are common biomedical materials owing to their combination of mechanical properties, corrosion resistance and biocompatibility. Powder metallurgy (PM) techniques can be used to fabricate biomaterials with tailored properties because changing the processing parameters, such as the sintering temp...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2016.12.005

    authors： Bolzoni L,Ruiz-Navas EM,Gordo E

    更新日期：2017-03-01 00:00:00

  • The posterior eye with age-related macular degeneration has isotropic and nonlinear viscoelastic properties.

    abstract：:Here we characterize and compare the anisotropic and nonlinear viscoelastic properties of the posterior eye of advanced dry age-related macular degeneration (AMD) patients and age-matched normal subjects. Ten normal horizontal, ten normal vertical, ten AMD horizontal, and ten AMD vertical strips of the macular retina ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2020.104207

    authors： He Y,Guo Y,Wang J,Lv W,Li X,Chen K

    更新日期：2021-02-01 00:00:00

  • Layer-specific residual deformations and uniaxial and biaxial mechanical properties of thoracic porcine aorta.

    abstract：:In this paper we hypothesize that the layer-separated residual stresses and mechanical properties of layer-separated thoracic aorta arteries may be dependent on arterial location of the vessel. To demonstrate any possible position differences, we measured the axial pre-stretch and opening angle and performed uniaxial ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2015.05.024

    authors： Peña JA,Martínez MA,Peña E

    更新日期：2015-10-01 00:00:00

  • Reproducibility assessment of ultrasound-based aortic stiffness quantification and verification using Bi-axial tensile testing.

    abstract：:Current guidelines for abdominal aortic aneurysm (AAA) repair are primarily based on the maximum diameter. Since these methods lack robustness in decision making, new image-based methods for mechanical characterization have been proposed. Recently, time-resolved 3D ultrasound (4D US) in combination with finite element...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2019.103571

    authors： van Disseldorp EMJ,van den Hoven MHMH,van de Vosse FN,van Sambeek MRHM,Lopata RGP

    更新日期：2020-03-01 00:00:00

  • Mechanical behavior of cells in microinjection: a minimum potential energy study.

    abstract：:Microinjection is a widely used technique to deliver foreign materials into biological cells. We propose a mathematical model to study the mechanical behavior of a cell in microinjection. Firstly, a cell is modeled by a hyperelastic membrane and interior cytoplasm. Then, based on the fact that the equilibrium configur...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2013.04.017

    authors： Liu F,Wu D,Chen K

    更新日期：2013-08-01 00:00:00

  • Collagen tissue treated with chitosan solution in H2O/CO2 mixtures: Influence of clathrates hydrates on the structure and mechanical properties.

    abstract：:A mixture of water/carbon dioxide is a "green" perspective solvent from the viewpoint of biomedical applications. Clathrate hydrates are formed this solvent under certain conditions and a very interesting question is the impact of clathrates hydrates on the structure and properties of bovine pericardium, which is used...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2016.11.015

    authors： Chaschin IS,Bakuleva NP,Grigoriev TE,Krasheninnikov SV,Nikitin LN

    更新日期：2017-03-01 00:00:00

  • Vancomycin elution, activity and impact on mechanical properties when added to orthopedic bone cement.

    abstract：:Infection incidence for total hip and knee arthroplasty (THA and TKA, respectively) is between 0.2% and 5% and results in approximately 100,000 device failures per year in the United States. Treatment requires prolonged systemic antibiotic therapy with additional surgical revisions. As a prophylactic measure against i...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2018.06.033

    authors： Bishop AR,Kim S,Squire MW,Rose WE,Ploeg HL

    更新日期：2018-11-01 00:00:00

  • Intrafibrillar plasticity through mineral/collagen sliding is the dominant mechanism for the extreme toughness of antler bone.

    abstract：:The inelastic deformability of the mineralised matrix in bones is critical to their high toughness, but the nanoscale mechanisms are incompletely understood. Antler is a tough bone type, with a nanostructure composed of mineralised collagen fibrils ∼100nm diameter. We track the fibrillar deformation of antler tissue d...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2013.03.020

    authors： Gupta HS,Krauss S,Kerschnitzki M,Karunaratne A,Dunlop JW,Barber AH,Boesecke P,Funari SS,Fratzl P

    更新日期：2013-12-01 00:00:00

  • Transient behavior and relaxation of microcapsules with a cross-linked human serum albumin membrane.

    abstract：:Capsules consist of droplets enclosed by a membrane with shear resistant properties especially when fabricated by interfacial cross-linking. In many applications, the protection and release of the internal medium need to be strictly controlled. It is possible to tune the membrane mechanical properties by changing the ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2015.09.008

    authors： Gires PY,Barthès-Biesel D,Leclerc E,Salsac AV

    更新日期：2016-05-01 00:00:00

  • Effect of grinding with diamond-disc and -bur on the mechanical behavior of a Y-TZP ceramic.

    abstract：:This study compared the effects of grinding on the surface micromorphology, phase transformation (t→m), biaxial flexural strength and structural reliability (Weibull analysis) of a Y-TZP (Lava) ceramic using diamond-discs and -burs. 170 discs (15×1.2mm) were produced and divided into 5 groups: without treatment (Ctrl,...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.05.010

    authors： Pereira GK,Amaral M,Simoneti R,Rocha GC,Cesar PF,Valandro LF

    更新日期：2014-09-01 00:00:00

  • The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells.

    abstract：:Although mechanical cues are known to affect stem cell fate and mechanobiology, the significance of such stimuli on the osteogenic differentiation of human adipose stem cells (hASCs) remains unclear. In this study, we investigated the effect of long-term mechanical stimulation on the attachment, osteogenic differentia...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2017.04.016

    authors： Virjula S,Zhao F,Leivo J,Vanhatupa S,Kreutzer J,Vaughan TJ,Honkala AM,Viehrig M,Mullen CA,Kallio P,McNamara LM,Miettinen S

    更新日期：2017-08-01 00:00:00

  • Augmentation of core decompression with synthetic bone graft does not improve mechanical properties of the proximal femur.

    abstract：:Core decompression is a minimally invasive surgical technique used to treat patients with avascular necrosis of the femoral head. The procedure requires an entry hole in the lateral cortex of the femur which potentially leaves patients susceptible to subtrochanteric fractures. The purpose of this study was to determin...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2020.104263

    authors： Hockett SA,Sherrill JT,Self M,Mears SC,Barnes CL,Mannen EM

    更新日期：2021-03-01 00:00:00

  • Fracture origins in twenty-two dental alumina crowns.

    abstract：OBJECTIVES:The causes of in vivo fractures of all-ceramic dental crowns are not yet fully understood. The fracture origins often occur in the cervical margin in the approximal area, but the reason for this is unclear. The aim of this study was to evaluate the fracture origin of 22 of clinically-failed alumina crowns. ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2015.08.006

    authors： Øilo M,Quinn GD

    更新日期：2016-01-01 00:00:00

  • The interfascicular matrix enables fascicle sliding and recovery in tendon, and behaves more elastically in energy storing tendons.

    abstract：:While the predominant function of all tendons is to transfer force from muscle to bone and position the limbs, some tendons additionally function as energy stores, reducing the cost of locomotion. Energy storing tendons experience extremely high strains and need to be able to recoil efficiently for maximum energy stor...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2015.04.009

    authors： Thorpe CT,Godinho MSC,Riley GP,Birch HL,Clegg PD,Screen HRC

    更新日期：2015-12-01 00:00:00

  • Molecular dynamics simulation of mechanical behavior of osteopontin-hydroxyapatite interfaces.

    abstract：:Bone is characterized with an optimized combination of high stiffness and toughness. The understanding of bone nanomechanics is critical to the development of new artificial biological materials with unique properties. In this work, the mechanical characteristics of the interfaces between osteopontin (OPN, a noncollag...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.04.002

    authors： Lai ZB,Wang M,Yan C,Oloyede A

    更新日期：2014-08-01 00:00:00

  • Mechanical properties and in vitro bioactivity of injectable and self-setting calcium sulfate/nano-HA/collagen bone graft substitute.

    abstract：:Calcium sulfate hemihydrate (CSH) was introduced into the mineralized collagen (nHAC) to prepare an injectable and self-setting in situ bone graft substitute. The mechanical properties of materials, which are dependant on the L/S ratio, the content of nHAC and setting accelerator, were discussed based on the satisfyin...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2011.12.007

    authors： Hu NM,Chen Z,Liu X,Liu H,Lian X,Wang X,Cui FZ

    更新日期：2012-08-01 00:00:00

  • Effect of hydroxyapatite fillers on the mechanical properties and osteogenesis capacity of bio-based polyurethane composite scaffolds.

    abstract：:A newly designed hydroxyapatite-polyurethane (HA-PU) composite scaffold was prepared by polymerizing glyceride of castor oil (GCO) with isophorone diisocyanate (IPDI) and HA as fillers. The aim of this study was to determine the effect of HA fillers on the mechanical properties and osteogenesis capacity of the composi...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2018.08.028

    authors： Du J,Zuo Y,Lin L,Huang D,Niu L,Wei Y,Wang K,Lin Q,Zou Q,Li Y

    更新日期：2018-12-01 00:00:00

  • A method to develop mock arteries suitable for cell seeding and in-vitro cell culture experiments.

    abstract：:Sylgard((R)) is a biocompatible elastomer which has been widely used in biomedical applications including in simulations of the mechanical response of soft tissues and mechanotransduction investigations. In this study the effect of fabrication parameters including base to curing agent ratio and curing time on the mech...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2010.04.003

    authors： Colombo A,Zahedmanesh H,Toner DM,Cahill PA,Lally C

    更新日期：2010-08-01 00:00:00

  • Mechanical response of human abdominal walls ex vivo: Effect of an incisional hernia and a mesh repair.

    abstract：:The design of meshes for the treatment of incisional hernias could benefit from better knowledge of the mechanical response of the abdominal wall and how this response is affected by the implant. The aim of this study was to characterise the mechanical behaviour of the human abdominal wall. Abdominal walls were tested...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.07.002

    authors： Podwojewski F,Otténio M,Beillas P,Guérin G,Turquier F,Mitton D

    更新日期：2014-10-01 00:00:00

  • Monotonic and cyclic loading behavior of porous scaffolds made from poly(para-phenylene) for orthopedic applications.

    abstract：:Porous poly(para-phenylene) (PPP) scaffolds have tremendous potential as an orthopedic biomaterial; however, the underlying mechanisms controlling the monotonic and cyclic behavior are poorly understood. The purpose of this study was to develop a method to integrate micro-computed tomography (μCT), finite-element anal...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.10.004

    authors： Hoyt AJ,Yakacki CM,Fertig RS 3rd,Dana Carpenter R,Frick CP

    更新日期：2015-01-01 00:00:00

  • Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope.

    abstract：:The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2011.11.010

    authors： Zhou ZL,Ngan AH,Tang B,Wang AX

    更新日期：2012-04-01 00:00:00

  • Impaired mechanics and matrix metalloproteinases/inhibitors expression in female ascending thoracic aortic aneurysms.

    abstract：:We hypothesized that female gender may have a specific negative impact on the mechanical characteristics, composition, and expression of matrix metalloproteinases/tissue inhibitors (MMPs/TIMPs) in the wall of ascending thoracic aortic aneurysms (ATAAs). Degenerative ATAAs were resected from 35 patients (age: 67±2 year...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.02.015

    authors： Sokolis DP,Iliopoulos DC

    更新日期：2014-06-01 00:00:00

  • Measuring the compressive viscoelastic mechanical properties of human cervical tissue using indentation.

    abstract：:The human cervix is an important mechanical barrier in pregnancy which must withstand the compressive and tensile forces generated from the growing fetus. Premature cervical shortening resulting from premature cervical remodeling and alterations of cervical material properties are known to increase a woman׳s risk of p...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.01.016

    authors： Yao W,Yoshida K,Fernandez M,Vink J,Wapner RJ,Ananth CV,Oyen ML,Myers KM

    更新日期：2014-06-01 00:00:00

  • Study of indentation of a sample equine bone using finite element simulation and single cycle reference point indentation.

    abstract：:In an attempt to study the mechanical behavior of bone under indentation, methods of analyses and experimental validations have been developed, with a selected test material. The test material chosen is from an equine cortical bone. Stress-strain relationships are first obtained from conventional mechanical property t...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2014.11.020

    authors： Hoffseth K,Randall C,Hansma P,Yang HT

    更新日期：2015-02-01 00:00:00

  • Nanoindentation of the insertional zones of human meniscal attachments into underlying bone.

    abstract：:The fibrocartilagenous knee menisci are situated between the femoral condyles and tibia plateau and are primarily anchored to the tibia by means of four attachments at the anterior and posterior horns. Strong fixation of meniscal attachments to the tibial plateau provide resistance to extruding forces of the meniscal ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2008.10.005

    authors： Hauch KN,Oyen ML,Odegard GM,Haut Donahue TL

    更新日期：2009-08-01 00:00:00

  • Measurement of the shear modulus in thin-layered tissues using numerical simulations and shear wave elastography.

    abstract：:Measurement of mechanical properties of thin-layered tissues has broad applications in the diagnosis of several pathologies. Ultrasound shear wave elastography (SWE) measures the shear wave speed as a means of estimating the mechanical properties of tissues. However, the wave speed in thin-layered tissues is affected ...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2019.103502

    authors： Sadeghi S,Cortes DH

    更新日期：2020-02-01 00:00:00

  • Comparison of splinted and non-splinted superstructures of three implants placed in a mandibular distal extension model with missing teeth using modal analysis.

    abstract：:This study evaluated the effects of two types of superstructures (splinted crown and non-splinted crown) on four vibration characteristics (natural frequency, damping ratio (DAR), vectors in antiphase, maximum displacement (MDP)) by using modal analysis. These structures were fabricated on three implants placed in the...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2020.104050

    authors： Sasaki T,Nakata H,Suzuki A,Hada T,Kasugai S,Kuroda S

    更新日期：2020-12-01 00:00:00

  • Statistics in experimental studies on the human spine: Theoretical basics and review of applications.

    abstract：:Proper statistical analysis is essential in the research studies. In particular, as regards the in vitro testing of the lumbar spine, the criteria for the standardization have been extensively discussed but the use of statistics has not been reviewed. Unfortunately, cadaveric testing is a very difficult and complex ex...

    journal_title：Journal of the mechanical behavior of biomedical materials

    pub_type： 杂志文章

    doi：10.1016/j.jmbbm.2020.103862

    authors： Bassani T,Galbusera F

    更新日期：2020-10-01 00:00:00