Abstract：
OBJECTIVE:The bromodomain and extra-terminal (BET) family of proteins provides a scaffolding platform for the recruitment and tethering of transcription factors to acetylated chromatin, thereby modulating gene expression. In this study, we evaluated the efficacy of the BET-inhibitor PFI-1 to diminish AR/AR-V7 signaling and proliferation in castration-resistant prostate cancer cells. METHODS:Prostate-specific antigen and androgen receptor (AR) protein were quantified by means of two commercial ELISAs. Transactivation of the AR, AR-V7 and Q641X was determined by reporter gene assays. Cell proliferation was measured using a colorimetric MTT-assay. RESULTS:PFI-1 dose-dependently inhibited transactivation of full-length AR (non- mutated, i.e., wild-type or point-mutated/promiscuous forms) without affecting their cellular protein levels. Moreover, PFI-1 was active against C-terminally truncated constitutively active ARs like AR-V7 and Q641X. Prostate cancer cells exhibiting a transcriptionally active AR-signaling complex (LNCaP, 22Rv1) were more susceptible to the growth-inhibitory effects than the AR-negative PC-3 cells. CONCLUSION:The quinazolinone PFI-1 is a highly efficient inhibitor of AR-signaling-competent prostate cancer cells in vitro. PFI-1 could serve as a lead compound for the development of new therapeutics able to block AR/AR-V7 signaling in advanced prostate cancer.
journal_name
World J Uroljournal_title
World journal of urologyauthors
Hupe MC,Hoda MR,Zengerling F,Perner S,Merseburger AS,Cronauer MVdoi
10.1007/s00345-018-2382-8subject
Has Abstractpub_date
2019-02-01 00:00:00pages
343-349issue
2eissn
0724-4983issn
1433-8726pii
10.1007/s00345-018-2382-8journal_volume
37pub_type
杂志文章abstract：:The diagnosis of prostate cancer is based on histology. Prostate biopsies are obtained based on the triad of prostate specific antigen (PSA), digital rectal examination (DRE) and transrectal ultrasound. Because prostate biopsies still have a large percentage of negative outcomes, patient selection and biopsy direction...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-007-0189-0
更新日期：2007-08-01 00:00:00
abstract：INTRODUCTION:Changes in the management of minimally invasive oncologic renal surgery have introduced ablative therapies as the most recent advancement in minimally invasive technology. METHODS:The current evidence-based medicine on the topic of laparoscopic (LCA) and percutaneous (PCA) renal cryoablation was gathered ...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-010-0552-4
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVES:It has been reported that apoptosis of penile erectile tissue occurs after penile denervation, castration, and diabetes mellitus in animal studies. Aim of this study was to investigate apoptosis in corpora cavernosa of patients with organic erectile dysfunction (ED). METHODS:Cavernous biopsies were obtained...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-008-0332-6
更新日期：2009-04-01 00:00:00
abstract：:Due to its unique biological behavior, late relapse (LR) of testicular cancer has recently been described as an own tumor entity. It is currently defined as tumor recurrence more than 2 years after complete remission following primary treatment including chemotherapy. The incidence ranges from 2 to 6%, with a median r...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-004-0397-9
更新日期：2004-04-01 00:00:00
abstract：PURPOSE:To examine patterns of crowdsourcing on the social media platform Twitter among urologists. METHODS:Urologists' public Twitter accounts were reviewed for original posts seeking clinical advice or feedback, and associated reply posts, before and after the 140-character-limit expansion in 2017. Predictors of res...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-018-2405-5
更新日期：2019-03-01 00:00:00
abstract：PURPOSE:Combination therapy of male lower urinary tract symptoms with α(1)-adrenoceptor and muscarinic receptor antagonists attracts increasing interest. Propiverine is a muscarinic receptor antagonist possessing additional properties, i.e., block of L-type Ca(2+) channels. Here, we have investigated whether propiverin...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-011-0655-6
更新日期：2011-04-01 00:00:00
abstract：PURPOSE:To investigate the inhibitory effects of electrical stimulation of sacral dorsal root ganglion (DRG) on bladder activity under non-nociceptive and nociceptive bladder conditions in cats. MATERIALS AND METHODS:12 cats were divided into non-nociceptive and nociceptive groups. Saline was used to distend the bladd...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-018-2198-6
更新日期：2018-05-01 00:00:00
abstract：PURPOSE:To perform a feasibility phase study of a panel of putative protein biomarkers and determine whether it can identify and predict tumor recurrence in patients with non-muscle-invasive bladder cancer (NMIBC) on follow-up. METHODS:We prospectively analyzed the urine of 152 patients previously treated for NMIBC. Q...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-020-03188-x
更新日期：2020-04-06 00:00:00
abstract：PURPOSE:Lymph node dissection (LND) is not routinely performed during radical nephroureterectomy (RNU) in upper tract urothelial carcinomas (UTUC), and its clinical relevance is unclear. The purpose of the present study was to evaluate the impact of LND on clinical outcomes in a large multicenter series of RNU for UTUC...
journal_title：World journal of urology
pub_type： 杂志文章,多中心研究
doi：10.1007/s00345-011-0705-0
更新日期：2011-08-01 00:00:00
abstract：PURPOSE:Although the role of lymph node dissection (LND) is well defined in many urological settings, uncertainty regarding need for LND still exists for patients with renal cell carcinoma (RCC). The aim of the current review is to highlight the rationale for performing or omitting LND at the time of renal surgery. ME...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-016-1886-3
更新日期：2017-04-01 00:00:00
abstract：:The diagnosis of prostate cancer (PCa) can be challenging due to the limited performance of current diagnostic tests, including PSA, digital rectal examination and transrectal conventional US. Multiparametric MRI has improved PCa diagnosis and is recommended prior to biopsy; however, mp-MRI does miss a substantial num...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-020-03193-0
更新日期：2020-04-18 00:00:00
abstract：PURPOSE:Radical cystectomy (RC) can be associated with significant blood loss. Allogenic blood transfusion (ABT) may alter disease outcome because of a theoretical immunomodulatory effect. We evaluated the effects of ABT on overall survival (OS) and progression-free survival (PFS) of patients undergoing RC for urotheli...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-014-1257-x
更新日期：2014-12-01 00:00:00
abstract：:Contemporary literature regarding the management of neobladder-vaginal fistula and stress urinary incontinence following radical cystectomy and neobladder reconstruction in women is reviewed in this article. Neobladder-vaginal fistula is uncommon but mandates meticulous repair. Compared to the native bladder, the wall...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-005-0013-7
更新日期：2005-09-01 00:00:00
abstract：OBJECTIVE:To assess the oncological safety of laparoscopic procedures for the management of urothelial carcinomas of the urinary tract. METHODS:Data on laparoscopic management of urothelial carcinomas in the literature were analysed using MEDLINE and by matching the following keywords: urological malignancies, upper t...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-008-0349-x
更新日期：2009-02-01 00:00:00
abstract：:The nuclear factor of kappa beta (NF-kappaB) transcription factor regulates the transcription of numerous genes including that of interleukin 6 (IL-6). The IL-6 acts as an autocrine and paracrine growth factor of androgen-independent prostate cancer. An aberrant expression of the IL-6 gene and an increase in IL-6 expr...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-007-0175-6
更新日期：2007-10-01 00:00:00
abstract：PURPOSE:Prostate Imaging Reporting and Data System version 2 (PI-RADSv2) provides reasonable performance in detecting significant cancers. Still, it is unclear about whether all PI-RADS 4 lesions show the same cancer detection rate (CDR) regardless of tumor size. The aim was to compare the CDRs of small (< 10 mm) and l...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-019-02924-2
更新日期：2020-06-01 00:00:00
abstract：INTRODUCTION:Currently, osteoporosis research is rarely undertaken in males but an increase in male life expectancy in the company of hypogonadism suggests the necessity for potential therapeutic options. MATERIALS AND METHODS:In this study, the changes in bone structure under standardized testosterone- (T), raloxifen...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-009-0373-5
更新日期：2009-08-01 00:00:00
abstract：PURPOSE:The introduction of collagenase Clostridium histolyticum (CCH) has revolutionized the treatment of Peyronie's disease. The efficacy of this therapy has been well demonstrated and the safety profile is favorable. However, post-injection complications are poorly characterized and management of these complications...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-019-02817-4
更新日期：2020-02-01 00:00:00
abstract：:The purpose of this study was to assess the ability of [(18)F]FDG-PET, CT/MRI and serum tumor marker (TM) to predict the viability of residual masses after high-dose chemotherapy (HD-Ctx) in patients with metastatic germ cell tumors (GCT). In a prospective study, 60 residual tumors in 28 GCT patients were classified a...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-003-0392-6
更新日期：2004-06-01 00:00:00
abstract：:Our objectives were to assess the value of extracorporeal shock wave lithotripsy (ESWL) in treating pediatric urolithiasis, and to determine the factors that may affect treatment success. Between January 1993 and August 2002, 129 children with upper urinary tract calculi (134 renoureteral units) were treated using a D...
journal_title：World journal of urology
pub_type： 临床试验,杂志文章
doi：10.1007/s00345-003-0385-5
更新日期：2004-06-01 00:00:00
abstract：:Surgery following chemotherapy for treatment of metastatic testis cancer is reserved for partial remissions with localized tumors considered resectable. After primary chemotherapy, about 90% will have teratoma or necrosis and only 10% will have cancer. The concept of two cycles of post operative chemotherapy in this s...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/BF00185671
更新日期：1994-01-01 00:00:00
abstract：:Candiduria is commonly seen in hospitalized patients and most of the patients are asymptomatic, but it may be due to cystitis, pyelonephritis, prostatitis, epididymo-orchitis or disseminated candidiasis. Major risk factors are diabetes mellitus, indwelling urinary catheters, use of broad-spectrum antibiotics, urinary ...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-019-02991-5
更新日期：2020-11-01 00:00:00
abstract：PURPOSE:To determine the clinical performance of a blood-based test for clinically significant (CS) prostate cancer (PCa) (grade group ≥ 2) intended for use in men with prostate serum antigen levels in the 'grey zone' (PSA < 10 ng/ml). The test quantifies a previously described 3.4 kb mitochondrial DNA (mtDNA) deletion...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-017-2152-z
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:To report the surgical outcomes of laparoscopic radical cystectomy (LRC) with extracorporeal orthotopic ileal neobladder (OIN) in patients with muscle-invasive urothelial carcinoma of the bladder (UCB). MATERIALS AND METHODS:Between October 2009 and December 2011, 37 patients with muscle-invasive UCB underw...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-013-1122-3
更新日期：2014-04-01 00:00:00
abstract：PURPOSE:To examine the prevalence of lower urinary tract symptoms (LUTS) and the bother they impose in a population-based sample of adults in Brazil. METHODS:A cross-sectional population-based survey was conducted between September 2006 and January 2007 in the city of Salvador, Brazil. Cluster samples of representativ...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-013-1057-8
更新日期：2013-12-01 00:00:00
abstract：:The aim of this study was to determine and compare the effects of both magnesium citrate and phytin on reducing urinary calcium excretion under high-calcium-diet conditions during single and combined treatments. An animal experiment was carried out over a period of 4 weeks in 35 male rats. Urinary calcium excretion wa...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/BF00184113
更新日期：1994-01-01 00:00:00
abstract：OBJECTIVES:Nocturia is prevalent and bothersome in men with lower urinary tract symptoms suggestive of BPH (LUTS/BPH). α-Adrenoceptor antagonists without subtype selectivity have inconsistently shown significant effects on nocturia in these patients. We explored the effects of the α1A-adrenoceptor subtype-selective ant...
journal_title：World journal of urology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00345-013-1228-7
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To assess treatments and survival of patients with muscle invasive bladder cancer (MIBC) in the Comprehensive Cancer Center Northern Netherlands (CCCN) region. STUDY DESIGN AND SETTING:Retrospective cohort analysis. Data of 548 patients with MIBC diagnosed between 1997 and 2002 were collected from the CCCN c...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-010-0546-2
更新日期：2010-08-01 00:00:00
abstract：:Prostate carcinoma (PCa) displays a wide variety of genetic alterations, versatile expression profiles as well as cell surface markers. Despite this heterogeneity, a common treatment for advanced PCa is androgen deprivation therapy (ADT). ADT targets the androgen receptor-a member of the nuclear receptor superfamily-w...
journal_title：World journal of urology
pub_type： 杂志文章,评审
doi：10.1007/s00345-011-0792-y
更新日期：2012-06-01 00:00:00
abstract：PURPOSE:Because there is a lack of evidence, it is not generally recommended to use adjuvant radiotherapy plus chemotherapy to treat lymph node disease in penile cancer. The aim of this study was to determine the benefit of using adjuvant radiotherapy after inguinal surgery for penile cancer. METHODS:Multi-institution...
journal_title：World journal of urology
pub_type： 杂志文章
doi：10.1007/s00345-020-03179-y
更新日期：2020-04-17 00:00:00
