Bioengineered Tooth Buds Exhibit Features of Natural Tooth Buds.

Abstract:

:Tooth loss is a significant health issue currently affecting millions of people worldwide. Artificial dental implants, the current gold standard tooth replacement therapy, do not exhibit many properties of natural teeth and can be associated with complications leading to implant failure. Here we propose bioengineered tooth buds as a superior alternative tooth replacement therapy. We describe improved methods to create highly cellularized bioengineered tooth bud constructs that formed hallmark features that resemble natural tooth buds such as the dental epithelial stem cell niche, enamel knot signaling centers, transient amplifying cells, and mineralized dental tissue formation. These constructs were composed of postnatal dental cells encapsulated within a hydrogel material that were implanted subcutaneously into immunocompromised rats. To our knowledge, this is the first report describing the use of postnatal dental cells to create bioengineered tooth buds that exhibit evidence of these features of natural tooth development. We propose future bioengineered tooth buds as a promising, clinically relevant tooth replacement therapy.

journal_name

J Dent Res

authors

Smith EE,Angstadt S,Monteiro N,Zhang W,Khademhosseini A,Yelick PC

doi

10.1177/0022034518779075

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

1144-1151

issue

10

eissn

0022-0345

issn

1544-0591

journal_volume

97

pub_type

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