Abstract:
:Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen colonization using traditional broad-spectrum agro-chemical protocols are less effective than desired. In the current study, we developed species-specific RNA interference-based control treatments capable of reducing fungal infection. Development of a target identification pipeline using global RNA sequencing data for selection and application of double stranded RNA (dsRNA) molecules identified single gene targets of the fungus. Using this approach, we demonstrate the utility of this technology through foliar applications of dsRNAs to the leaf surface that significantly decreased fungal infection and S. sclerotiorum disease symptoms. Select target gene homologs were also tested in the closely related species, Botrytis cinerea, reducing lesion size and providing compelling evidence of the adaptability and flexibility of this technology in protecting plants against devastating fungal pathogens.
journal_name
Sci Repjournal_title
Scientific reportsauthors
McLoughlin AG,Wytinck N,Walker PL,Girard IJ,Rashid KY,de Kievit T,Fernando WGD,Whyard S,Belmonte MFdoi
10.1038/s41598-018-25434-4subject
Has Abstractpub_date
2018-05-09 00:00:00pages
7320issue
1issn
2045-2322pii
10.1038/s41598-018-25434-4journal_volume
8pub_type
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