Abstract:
:In this study, we assessed the role of different reactive oxygen species (ROS) generated by soft jet plasma and chemical-induced ROS systems with regard to cell death in T98G, A549, HEK293 and MRC5 cell lines. For a comparison with plasma, we generated superoxide anion (O2(-)), hydroxyl radical (HO·), and hydrogen peroxide (H2O2) with chemicals inside an in vitro cell culture. Our data revealed that plasma decreased the viability and intracellular ATP values of cells and increased the apoptotic population via a caspase activation mechanism. Plasma altered the mitochondrial membrane potential and eventually up-regulated the mRNA expression levels of BAX, BAK1 and H2AX gene but simultaneously down-regulated the levels of Bcl-2 in solid tumor cells. Moreover, a western blot analysis confirmed that plasma also altered phosphorylated ERK1/2/MAPK protein levels. At the same time, using ROS scavengers with plasma, we observed that scavengers of HO· (mannitol) and H2O2 (catalase and sodium pyruvate) attenuated the activity of plasma on cells to a large extent. In contrast, radicals generated by specific chemical systems enhanced cell death drastically in cancer as well as normal cell lines in a dose-dependent fashion but not specific with regard to the cell type as compared to plasma.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kaushik N,Uddin N,Sim GB,Hong YJ,Baik KY,Kim CH,Lee SJ,Kaushik NK,Choi EHdoi
10.1038/srep08587subject
Has Abstractpub_date
2015-02-26 00:00:00pages
8587issn
2045-2322pii
srep08587journal_volume
5pub_type
杂志文章abstract::The biliary tree is an essential component of transplantable human liver tissue. Despite recent advances in liver tissue engineering, attempts at re-creating the intrahepatic biliary tree have not progressed significantly. The finer branches of the biliary tree are structurally and functionally complex and heterogeneo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30433-6
更新日期:2018-08-15 00:00:00
abstract::High altitude pulmonary edema (HAPE) susceptibility is associated with EGLN1 polymorphisms, we hypothesized that HAPE-susceptible (HAPE-S, had HAPE episode in past) subjects may exhibit abnormal HIF1α levels in normoxic conditions. We measured HIF1α levels in HAPE-S and HAPE resistant (HAPE-R, no HAPE episode) individ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26468
更新日期:2016-05-23 00:00:00
abstract::Our study aimed to investigate the effect of Pap smear screening on stage at diagnosis of cervical cancer in a heterogeneous population of Thai women. Data was merged from the population-based cancer registry and screening registry based on unique identification numbers from 2006 to 2014. Patients being screened had l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52607-6
更新日期:2019-11-15 00:00:00
abstract::The ultimate goal of epileptology is the complete abolishment of epileptic seizures. This might be achieved by a system that predicts seizure onset combined with a system that interferes with the process that leads to the onset of a seizure. Seizure prediction remains, as of yet, unresolved in absence-epilepsy, due to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02626-y
更新日期:2017-05-29 00:00:00
abstract::Multigene expression signatures provide a molecular subdivision of early breast cancer associated with patient outcome. A gap remains in the validation of such signatures in clinical treatment groups of patients within population-based cohorts of unselected primary breast cancer representing contemporary disease stage...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48570-x
更新日期:2019-08-21 00:00:00
abstract::Macrophage infiltration and recruitment in breast tumors has been correlated with poor prognosis in breast cancer patients and has been linked to tumor cell dissemination. Much of our understanding comes from animal models in which macrophages are labeled by expression of an extrinsic fluorophore. However, conventiona...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25086
更新日期:2016-05-25 00:00:00
abstract::Aedes aegypti is the primary vector for transmission of Dengue, Zika and chikungunya viruses. Previously it was shown that Dengue virus infection of the mosquito led to an in increased expression of the odorant binding protein 22 (AeOBP22) within the mosquito salivary gland and that siRNA mediated knockdown of AeOBP22...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60242-9
更新日期:2020-02-24 00:00:00
abstract::A two-dimensional electron gas (2DEG) with equal-strength Rashba and Dresselhaus spin-orbit coupling sustains persistent helical spin-wave states, which have remarkably long lifetimes. In the presence of an in-plane magnetic field, there exist single-particle excitations that have the character of propagating helical ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21818-8
更新日期:2018-02-22 00:00:00
abstract::Mortality of glioblastoma multiforme (GBM) has not improved over the last two decades despite medical breakthroughs in the treatment of other types of cancers. Nanoparticles hold tremendous promise to overcome the pharmacokinetic challenges and off-target adverse effects. However, an inhibitory effect of nanoparticles...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59146-5
更新日期:2020-02-11 00:00:00
abstract::Condensin complexes play crucial roles in chromosome condensation that is a fundamental process to establish the "rod-like" shape of chromosome structure in mitosis. Failure of the chromosome assembly causes chromosome segregation errors and subsequent genomic instability. However, a molecular mechanism that controls ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05986-7
更新日期:2017-07-17 00:00:00
abstract::Developing an effective universal influenza vaccine against influenza virus with highly conserved antigenic epitopes could induce a broad-spectrum immune response to prevent infection. The soluble protein M1 that can induce the M1 specific immune response was first confirmed in our previous study. In this study, we ch...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60783-z
更新日期:2020-03-05 00:00:00
abstract::RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro. However, little is known about its tissue tropism and persistence of gene expression in vivo. Here, to evaluate the feasibility of REVec for in vivo gene delivery...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62630-7
更新日期:2020-04-03 00:00:00
abstract::There has been a developing technology in algae with pharmaceuticals wastewater. However, the effect and the underlying mechanism of pharmaceuticals on algae are not well understood. To investigate the effect and mechanism of pharmaceuticalson microalgae, four pharmaceuticals of clofibric acid (CLF), ciprofloxacin (CI...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36609-4
更新日期:2019-02-07 00:00:00
abstract::The crystal structure of the Sb6O13 oxide, exhibiting a defect pyrochlore crystal structure with atomic vacancies, has been studied using a complete set of state-of-the-art techniques. The degree of antimony disproportionation in Sb3+ and Sb5+ valence states has been directly determined around 36% and 64%, respectivel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73860-0
更新日期:2020-10-12 00:00:00
abstract::Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements - it is therefore important to determine if they favor outgrowth of d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05207-1
更新日期:2017-07-11 00:00:00
abstract::Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76932-3
更新日期:2020-11-18 00:00:00
abstract::An effective method, involving time and frequency domains was developed to interpret seismic precursors by comparing groundwater-level fluctuations recorded immediately and long before the occurrence of a known earthquake. The proposed method, consisting of the pre-processing (3-point filtering, band-pass filtering, a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64586-0
更新日期:2020-05-12 00:00:00
abstract::The origin and domestication of cultivated barley have long been under debate. A population-based resequencing and phylogenetic analysis of the single copy of RPB2 gene was used to address barley domestication, to explore genetic differentiation of barley populations on the worldwide scale, and to understand gene-pool...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36122
更新日期:2016-10-27 00:00:00
abstract::New monosaccharide-based lipid A analogues were rationally designed through MD-2 docking studies. A panel of compounds with two carboxylate groups as phosphates bioisosteres, was synthesized with the same glucosamine-bis-succinyl core linked to different unsaturated and saturated fatty acid chains. The binding of the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37421-w
更新日期:2019-01-29 00:00:00
abstract::It has been demonstrated that development of three-dimensional printing technology has supported the researchers and surgeons to apply the bone tissue engineering to the oromandibular reconstruction. In this study, poly caprolactone/beta tricalcium phosphate (PCL/β-TCP) scaffolds were fabricated by multi-head depositi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61944-w
更新日期:2020-03-18 00:00:00
abstract::The early detection of cognitive impairment or dementia is in the focus of current research as the amount of cognitively impaired individuals will rise intensely in the next decades due to aging population worldwide. Currently available diagnostic tools to detect mild cognitive impairment (MCI) or dementia are time-co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40010-0
更新日期:2019-03-05 00:00:00
abstract::Although the glucose lowering effect of dipeptidyl peptidase-4 (DPP4) inhibitors is well established, several potential serious acute safety concerns have been raised including acute kidney injury, respiratory tract infections, and acute pancreatitis. Using the UK-based Clinical Practice Research Datalink (CPRD), we i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33483-y
更新日期:2018-10-11 00:00:00
abstract::Macular edema due to neovascular age-related macular degeneration, diabetes or retinal vein occlusion can cause central vision loss. Intravitreal treatment with antibody-based biopharmaceutical compounds designed to neutralize vascular endothelial growth factor (VEGF) has proven to be an efficient strategy to ameliora...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20525-8
更新日期:2018-02-01 00:00:00
abstract::Epigenetics alternation of non-genetic variation and genome-wide association study proven allelic variants may associate with insulin secretion in type 2 diabetes (T2D) development. We analyzed promoter DNA methylation array to evaluate the associated with increased susceptibility to T2D (30 cases, 10 controls) and fo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71336-9
更新日期:2020-09-30 00:00:00
abstract::Micro-tube experiment has been implemented to understand the mechanisms of governing microcosmic fluid percolation and is extensively used in both fields of micro electromechanical engineering and petroleum engineering. The measured pressure difference across the microtube is not equal to the actual pressure differenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16870-9
更新日期:2017-11-29 00:00:00
abstract::While cerebrospinal fluid (CSF) biomarkers for Creutzfeldt-Jakob disease (CJD) are established and partly included in the diagnostic criteria, no blood biomarkers are available. Here, we assessed the utility of serum neurofilament light chain (NF-L) and tau protein in comparison to CSF markers (NF-L and phosphorylated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38737
更新日期:2016-12-08 00:00:00
abstract::In the field of neuroprosthetics, the current state-of-the-art method involves controlling the prosthesis with electromyography (EMG) or electrooculography/electroencephalography (EOG/EEG). However, these systems are both expensive and time consuming to calibrate, susceptible to interference, and require a lengthy lea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73250-6
更新日期:2020-10-01 00:00:00
abstract::Conventional solid lubricants such as MoS2, graphite, or diamond-like carbon films demonstrate excellent tribological performance but only in specific environments due to their inherent materials properties. This limitation prohibits using these solid lubricants in environments that change dynamically. This study pres...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72666-4
更新日期:2020-09-21 00:00:00
abstract::Untreatable bacterial infections caused by a perpetual increase of antibiotic resistant strains represent a serious threat to human healthcare in the 21(st) century. Conjugative DNA transfer is the most important mechanism for antibiotic resistance and virulence gene dissemination among bacteria and is mediated by a p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24643
更新日期:2016-04-22 00:00:00
abstract::Plasmonics is a rapidly emerging platform for quantum state engineering with the potential for building ultra-compact and hybrid optoelectronic devices. Recent experiments have shown that despite the presence of decoherence and loss, photon statistics and entanglement can be preserved in single plasmonic systems. This...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18313
更新日期:2015-12-16 00:00:00