Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair.

Abstract:

PURPOSE OF REVIEW:Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts. RECENT FINDINGS:Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.

journal_name

Curr Cardiol Rep

authors

Ding Y,Lee J,Hsu JJ,Chang CC,Baek KI,Ranjbarvaziri S,Ardehali R,Packard RRS,Hsiai TK

doi

10.1007/s11886-018-0979-6

subject

Has Abstract

pub_date

2018-03-24 00:00:00

pages

35

issue

5

eissn

1523-3782

issn

1534-3170

pii

10.1007/s11886-018-0979-6

journal_volume

20

pub_type

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