Abstract：
:Calf lenses which are incubated in solutions of 1-150 mM H2O2 for 24 hr remain clear at 20 degrees C. While insoluble lens protein increases by at most 2-3%, we find extensive oxidation of exposed protein thiols, major shifts in the size distribution of crystallins, and progressive generation of more acidic polypeptides. Some of these oxidative modifications are reversible with reducing agent. beta H-Crystallins are particularly susceptible to oxidation: disulfide-bonded soluble aggregates form at low H2O2 levels, while irreversible dissociation to beta L-crystallins occurs at high H2O2 concentration. The gamma-crystallins are particularly prone to charge modification. In contrast, the size and charge distributions of alpha-crystallins appear to be virtually unaffected by H2O2.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Siezen RJ,Coppin CM,Kaplan ED,Dwyer D,Thomson JAdoi
10.1016/s0014-4835(89)80072-7subject
Has Abstractpub_date
1989-02-01 00:00:00pages
225-35issue
2eissn
0014-4835issn
1096-0007journal_volume
48pub_type
杂志文章abstract：:Ischaemia/reperfusion contributes to the pathophysiological process of many retinal diseases. Previous studies have shown that retinal ischaemia/reperfusion mainly results in neuronal degeneration, including thinning of the retina, retinal ganglion cell death and reductions in electroretinography. A high-salt diet con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107784
更新日期：2019-11-01 00:00:00
abstract：:Previous histochemical and biochemical studies have documented the presence of carbohydrate-containing molecules in the retinal interphotoreceptor matrix (IPM). The lectin peanut agglutinin (PNA), which preferentially binds galactose-containing carbohydrates, especially galactose-galactosamine linkages, selectively la...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80163-x
更新日期：1987-04-01 00:00:00
abstract：:Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:We describe a model of acute intraocular pressure (IOP) elevation in the mouse eye that induces reversible loss of inner retinal function associated with oxidative stress, glial cell activation and minimal loss of retinal ganglion cell (RGC) number. Young healthy mouse eyes recover inner retinal function within 7-days...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.03.006
更新日期：2015-12-01 00:00:00
abstract：:Rhegmatogenous retinal detachment is by far the most common indication for retinal surgery and a major cause of severe vision loss. Increased levels of glutamate found in the vitreous of human patients and persistent remodeling, even after reattachment, suggest substantial neurochemical, functional and anatomical chan...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.02.009
更新日期：2012-04-01 00:00:00
abstract：:Patients with inflammatory bowel disease suffer not only from gut inflammation, but also from extraintestinal manifestations of the disease, including ocular pathology. The mechanisms causing ocular inflammation in these patients are unknown. The purpose of the current study was to investigate the possible vascular ch...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.023
更新日期：2013-10-01 00:00:00
abstract：:The proteasomal pathway is responsible for processes essential for cell viability, including the selective degradation of oxidized proteins. An age-dependent loss in proteasome function has been reported in many tissues, but has not been examined in the retina. In this study, we evaluated proteasome function and prote...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
abstract：:Potentiation of retinal degeneration by intense light exposure, and its amelioration by an antioxidant, were studied in a rat model of Smith-Lemli-Opitz syndrome (SLOS), in comparison with normal (control) Sprague-Dawley rats. The SLOS model is created by treating rats with AY9944, a selective inhibitor of cholesterol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.08.008
更新日期：2006-03-01 00:00:00
abstract：:The differentiation of rat lens epithelial cells to fibre cells can be mimicked using lens epithelial explants, which differentiate in vitro when exposed to fibroblast growth factor (FGF). A previous study demonstrated that FGF is required only for initiation of differentiation: once induced by FGF, differentiation ca...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0534
更新日期：1998-10-01 00:00:00
abstract：:The purpose of this investigation was to explore the combined effects of single nucleotide polymorphisms (SNPs) within LFA-1/ICAM-1/GSK-3β pathway and environmental hazards on susceptibility to Graves' opthalmopathy (GO) among a Chinese Han population. Altogether 305 GO patients and 283 Graves' disease (GD) subjects w...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.022
更新日期：2018-09-01 00:00:00
abstract：:Diabetic retinopathy (DR) is the most common cause of blindness in the working age population. Early events of DR are accompanied by neurodegeneration of the inner retina resulting in ganglion cell loss. These findings together with reduced retinal thickness are observed within the first weeks of experimental DR. Besi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.013
更新日期：2018-09-01 00:00:00
abstract：:As a result of longitudinal chromatic aberration (LCA), longer wavelengths are blurred when shorter wavelengths are in focus, and vice versa. As a result, LCA affects the color and temporal aspects of the retinal image with hyperopic defocus. In this experiment, we investigated how the sensitivity to temporal color co...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108331
更新日期：2021-01-01 00:00:00
abstract：:Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90088-c
更新日期：1990-11-01 00:00:00
abstract：:It has been speculated that the unitary eyes of vertebrates and molluscs, and the compound eyes of insects and crustaceans, evolved separately. On the other hand, the common use of rhodopsin as a photoreceptor molecule, and the conservation of Pax6 as a master control gene for eye development, suggest instead that the...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2018.05.007
更新日期：2019-06-01 00:00:00
abstract：:Ion channels and ion transporters play essential roles in the function of the retinal pigment epithelium (RPE). The use of cell cultures has been exploited as a key method for successfully identifying and studying ion channels and transporters of the RPE. Cultured RPE cells enable robust and long-lasting patch-clamp r...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2014.05.005
更新日期：2014-09-01 00:00:00
abstract：:Iron is an essential biometal in the aqueous humor, the principal source of nutrients for the avascular cornea and the lens. Here, we explored whether the ciliary body (CB), the source of aqueous humor, transports iron, and if the prion protein (PrPC) facilitates this process as in the outer retina. Using a combinatio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.031
更新日期：2018-10-01 00:00:00
abstract：:Extracts of bovine corneal stroma have been shown to activate keratocytes in culture to proliferate. We fractionated stromal extract on a column of Sephacryl S-300 and tested the fractions for mitogenic activity using cell culture and for the presence of IGF-II and its binding protein IGFBP-2 by Western blot. We found...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.12.004
更新日期：2008-03-01 00:00:00
abstract：:N-methyl-D-aspartate (NMDA)-induced excitotoxicity is an acute form of experimental retinal injury as a result of overactivation of glutamate receptors. NLRP3 (nucleotide-binding domain, leucine-rich-repeat containing family, pyrin domain containing-3) inflammasome, one of the most studied sensors of innate immunity, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.01.018
更新日期：2019-04-01 00:00:00
abstract：:The suitability of the rat derived SV-40T immortalized RPE-J cell line for identifying proteome changes associated with RPE differentiation was evaluated by surveying changes in protein expression levels. Rat RPE-J cells were induced to undergo differentiation in culture by growth at the nonpermissive temperature of 4...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1058
更新日期：2001-10-01 00:00:00
abstract：:Interleukin (IL)-1alpha and beta are important modulators of many functions of corneal epithelial and stromal cells that occur following injury to the cornea, including the influx of bone marrow-derived inflammatory cells into the stroma attracted by chemokines released from the stroma and epithelium. In this study, w...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.02.001
更新日期：2008-05-01 00:00:00
abstract：:Steady state photoreceptor specific mRNA levels in bovine retina were studied during fetal maturation for five gene transcripts, including rhodopsin, arrestin (S-antigen), rod alpha-subunit of transducin, interphotoreceptor retinoid-binding protein (IRBP) and rod alpha-subunit of cGMP phosphodiesterase in order to und...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1034
更新日期：1993-03-01 00:00:00
abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1175
更新日期：1993-12-01 00:00:00
abstract：:Small, experimental, non-rhegmatogenous retinal detachments (blebs) in rabbit eyes resorbed 50% more slowly when filled with autologous serum than with Hanks' solution. To study the fate of large molecules in the subretinal space, carboxyfluorescein and several sizes of FITC-dextrans were injected into blebs and their...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90138-1
更新日期：1985-05-01 00:00:00
abstract：:We investigated the inhibitory action of a synthetic peptidyl hydroxamate inhibitor of matrix metalloproteinase (MMP), Galardin (GM6001), on collagen degradation by rabbit corneal stromal fibroblasts (keratocytes) cultured three-dimensionally in the type I collagen gel with medium containing interleukin 1alpha (IL-1al...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0637
更新日期：1999-05-01 00:00:00
abstract：:Human, monkey, and bovine retinal pigment epithelial (RPE) cells exhibit an M-type K+ current, which in many other cell types is mediated by channels composed of KCNQ α-subunits and KCNE auxiliary subunits. Recently, we demonstrated the expression of KCNQ1, KCNQ4, and KCNQ5 in the monkey RPE. Here, we investigated the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:Herpes simplex virus type-1 (HSV-1) infection leads to impaired corneal sensation and, in severe cases, to corneal ulceration, melting and perforation. Here, we explore the potential therapeutic action of pigment epithelial-derived factor (PEDF) plus docosahexaenoic acid (DHA) on corneal inflammation and nerve regener...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.06.015
更新日期：2017-08-01 00:00:00
abstract：:Evidence is presented that alpha-crystallin isolated at 37 degrees C exists as a species, alpha m, which has a minimum molecular weight of about 320000 and a sedimentation coefficient of about 12 S. The amino acid composition, subunit distribution, near- and far-UV CD spectra and immunochemical properties were identic...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90173-2
更新日期：1983-10-01 00:00:00
abstract：:Research over the past two decades has shown that the growth of young eyes is guided by vision. If near- or far-sightedness is artificially imposed by spectacle lenses, eyes of primates and chicks compensate by changing their rate of elongation, thereby growing back to the pre-lens optical condition. Little is known a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0813
更新日期：2000-04-01 00:00:00
abstract：:Our previous gene expression analysis suggested that conjunctival epithelial cells of Sjögren's syndrome (SS) are inclined to hyper-proliferation and keratinisation status. The goal of this study is to elucidate whether such pathological situations really exist in the conjunctival epithelium of SS. Also, involvement o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.07.009
更新日期：2006-03-01 00:00:00
