Abstract：
:Mitotic fidelity is ensured by achieving biorientation on all paired chromosomes. The key signal for proper chromosome alignment is the tension between sister chromatids created by opposing poleward force from the spindles. In the budding yeast, the tension-sensing function requires that the Shugoshin protein, Shugoshin 1, be recruited to the centromeres and the neighboring pericentric regions. Concerted actions integrating proteins at centromeres and pericentromeres create highly specific Shugoshin 1 domains on mitotic chromosomes. We have previously reported that an important regulatory region on histone H3, termed the tension-sensing motif (TSM), is responsible for retaining Shugoshin 1 at pericentromeres. The TSM is negatively regulated by the acetyltransferase Gcn5p, but the underlying mechanism was elusive. In this work, we provide evidence that, when the TSM function is impaired, the histone H3 tail adopts a role that complements the damaged TSM to ensure faithful mitosis. This novel function of the H3 tail is controlled by Gcn5p, which targets selective lysine residues. Mutations to K14 and K23 ameliorate the mitotic defects resulting from TSM mutations. The restoration of faithful segregation is accompanied by regaining Shugoshin 1 access to the pericentric regions. Our data reveal a novel pathway for mitotic Shugoshin 1 recruitment and further reinforce the active role played by chromatins during their segregation in mitosis.
journal_name
Geneticsjournal_title
Geneticsauthors
Buehl CJ,Deng X,Luo J,Buranasudja V,Hazbun T,Kuo MHdoi
10.1534/genetics.117.300606subject
Has Abstractpub_date
2018-02-01 00:00:00pages
565-578issue
2eissn
0016-6731issn
1943-2631pii
genetics.117.300606journal_volume
208pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Mutants of Paramecium caudatum with abnormal swimming behavior or responses to cations were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Some of the mutants, like pawn in P. tetraurelia, cannot swim backward and are called CNR. Seven independently obtained CNR mutants belonged to three complement...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:Hairpin-tail (T(hp)) is a new allele of brachyury on chromosome 17 (linkage group IX) of the mouse. The morphological effects in the heterozygote are on the notochord and the tail. The homozygous abnormal is unknown. T(hp) is unique in that the phenotype of the heterozygote seems to depend on the source of the T(hp) g...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-04-01 00:00:00
abstract：:Mutations identified in zebrafish genetic screens allow the dissection of a wide array of problems in vertebrate biology. Most screens have examined mutations induced by treatment of spermatogonial (premeiotic) cells with the chemical mutagen N-ethyl-N-nitrosourea (ENU). Treatment of postmeiotic gametes with ENU induc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:The heat-shock response is a programmed change in gene expression carried out by cells in response to environmental stress, such as heat. This response is universal and is characterized by the synthesis of a small group of conserved protein chaperones. In Drosophila melanogaster the Hsp70 chaperone dominates the profi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.048793
更新日期：2006-01-01 00:00:00
abstract：:Sponsored by the National Science Foundation and the U.S. Department of Agriculture, a wheat genome sequencing workshop was held November 10-11, 2003, in Washington, DC. It brought together 63 scientists of diverse research interests and institutions, including 45 from the United States and 18 from a dozen foreign cou...
journal_title：Genetics
pub_type：
doi：10.1534/genetics.104.034769
更新日期：2004-10-01 00:00:00
abstract：:Microtubules are dynamic structures that must elongate, disassemble, and be cleaved into smaller pieces for proper neuronal development and function. The AAA ATPase Spastin severs microtubules along their lengths and is thought to regulate the balance between long, stable filaments and shorter fragments that seed exte...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.130831
更新日期：2011-09-01 00:00:00
abstract：:The parentals of the interspecific cross Cucurbita maxima xC. ecuadorensis had different isozyme phenotypes for 12 enzyme systems. Characterization of the systems demonstrated that the expression and intracellular distribution of the isozymes were similar to those in other plant taxa; however, a considerable number of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Many of the transcription factors and target genes that pattern the developing adult remain unknown. In the present study, we find that an ortholog of the poorly understood transcription factor, glucose transporter (GLUT4) enhancer factor (Glut4EF, GEF) [also known as the Huntington's disease gene regulatory region-bi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.078030
更新日期：2008-02-01 00:00:00
abstract：:The Caenorhabditis elegans gene gld-1 is essential for oocyte development; in gld-1 (null) hermaphrodites, a tumor forms where oogenesis would normally occur. We use genetic epistasis analysis to demonstrate that tumor formation is dependent on the sexual fate of the germline. When the germline sex determination pathw...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:To identify the factors (selective or mutational) that affect the distribution of transposable elements (TEs) within a genome, it is necessary to compare the pattern of newly arising element insertions to the pattern of element insertions that have been fixed in a population. To do this, we analyzed the distribution o...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:The genetic etiology of many complex diseases is highly heterogeneous. A complex disease can be caused by multiple mutations within the same gene or mutations in multiple genes at various genomic loci. Although these disease-susceptibility mutations can be collectively common in the population, they are often individu...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301266
更新日期：2018-10-01 00:00:00
abstract：:Obligate pathogenic and endosymbiotic bacteria typically experience gene loss due to functional redundancy, asexuality, and genetic drift. We hypothesize that reduced genomes increase their functional complexity through protein multitasking, in which many genes adopt new roles to counteract gene loss. Comparisons of i...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.145656
更新日期：2013-01-01 00:00:00
abstract：:The centromere is essential for proper segregation and inheritance of genetic information. Centromeres are generally regulated to occur exactly once per chromosome; failure to do so leads to chromosome loss or damage and loss of linked genetic material. The mechanism for faithful regulation of centromere activity and ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:An article from Alan Hinnebusch's laboratory in the March 2013 issue of GENETICS establishes an exciting new link between proteins with well-established roles in the endomembrane system and the process of transcription elongation. This Primer article provides tools needed for students to fully appreciate, analyze, and...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.150763
更新日期：2013-05-01 00:00:00
abstract：:We describe a unified set of methods for the inference of demographic history using genealogies reconstructed from gene sequence data. We introduce the skyline plot, a graphical, nonparametric estimate of demographic history. We discuss both maximum-likelihood parameter estimation and demographic hypothesis testing. S...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:The biological basis of exercise behavior is increasingly relevant for maintaining healthy lifestyles. Various quantitative genetic studies and selection experiments have conclusively demonstrated substantial heritability for exercise behavior in both humans and laboratory rodents. In the "High Runner" selection exper...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303668
更新日期：2020-11-01 00:00:00
abstract：:Inverted chromosome duplications or palindromes are linked with genetic disorders and malignant transformation. They are considered by-products of DNA double-strand break (DSB) repair: the homologous recombination (HR) and the nonhomologous end joining (NHEJ). Palindromes near chromosome ends are often triggered by te...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.183020
更新日期：2016-08-01 00:00:00
abstract：:We have isolated 15 spontaneous mutants resistant to one or several antibiotics like chloramphenicol, erythromycin and spiramycin. We have shown by several criteria that all of them result from mutations localized in the mitochondrial DNA. The mutations have been mapped by allelism tests and by two- and three-factor c...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-12-01 00:00:00
abstract：:Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (H...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.165613
更新日期：2014-09-01 00:00:00
abstract：:The paralogous maize proteins EMPTY PERICARP2 (EMP2) and HEAT SHOCK FACTOR BINDING PROTEIN2 (HSBP2) each contain a single recognizable motif: the coiled-coil domain. EMP2 and HSBP2 accumulate differentially during maize development and heat stress. Previous analyses revealed that EMP2 is required for regulation of hea...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.026575
更新日期：2004-07-01 00:00:00
abstract：:The impact of among-environment heteroscedasticity and genetic autocorrelation on the analysis of phenotypic plasticity is examined. Among-environment heteroscedasticity occurs when genotypic variances differ among environments. Genetic autocorrelation arises whenever the responses of a genotype to different environme...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:In the present study, we investigated intrachromosomal homologous recombination in a murine hybridoma in which the recipient for recombination, the haploid, endogenous chromosomal immunoglobulin mu-gene bearing a mutation in the constant (Cmu) region, was separated from the integrated single copy wild-type donor Cmu r...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:According to the ratchet mechanism hypothesis, deleterious mutations should accumulate in clonal genomes of unisexual fish of the genus Poeciliopsis. This study defines one such mutant, a silent carboxylesterase allele (ES-5 degrees) which is found in the heterozygous condition in a particular population of P. monacha...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:The sequentially Markov coalescent is a simplified genealogical process that aims to capture the essential features of the full coalescent model with recombination, while being scalable in the number of loci. In this article, the sequentially Markov framework is applied to the conditional sampling distribution (CSD), ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.125534
更新日期：2011-04-01 00:00:00
abstract：:The small heat-shock proteins have undergone a tremendous diversification in plants; whereas only a single small heat-shock protein is found in fungi and many animals, over 20 different small heat-shock proteins are found in higher plants. The small heat-shock proteins in plants have diversified in both sequence and c...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Highly specialized obligatory cave beetles endemic to the French Pyrenees offer an opportunity to investigate the relative importance of environmental conditions and ecological characteristics on the organization of genetic variability, to describe the genetic structure of populations, and to assess the extent of gene...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Crossover interference in meiosis is often modeled via stationary renewal processes. Here we consider a new model to incorporate the known biological feature of "obligate chiasma" whereby in most organisms each bivalent almost always has at least one crossover. The initial crossover is modeled as uniformly distributed...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.070235
更新日期：2007-07-01 00:00:00
abstract：:The present report is a study of 60 intragenic suppressors located in the A or E group of the b2 spore-color locus of Ascobolus immersus. The frameshift nature of the suppressors was shown by 19 combinations of plus and minus suppressor mutations. The location of the mutation sites on the genetic map of group A was de...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Hybridization between related species results in the formation of an allopolyploid with multiple subgenomes. These subgenomes will each contain complete, yet evolutionarily divergent, sets of genes. Like a diploid hybrid, allopolyploids will have two versions, or homeoalleles, for every gene. Partial functional redund...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301851
更新日期：2019-03-01 00:00:00
abstract：:Meiotically driven sex chromosomes can quickly spread to fixation and cause population extinction unless balanced by selection or suppressed by genetic modifiers. We report results of genetic analyses that demonstrate that extreme female-biased sex ratios in two sister species of stalk-eyed flies, Cyrtodiopsis dalmann...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
