Abstract:
:Microtubules are dynamic structures that must elongate, disassemble, and be cleaved into smaller pieces for proper neuronal development and function. The AAA ATPase Spastin severs microtubules along their lengths and is thought to regulate the balance between long, stable filaments and shorter fragments that seed extension or are transported. In both Drosophila and humans, loss of Spastin function results in reduction of synaptic connections and disabling motor defects. To gain insight into how spastin is regulated, we screened the Drosophila melanogaster genome for deletions that modify a spastin overexpression phenotype, eye size reduction. One suppressor region deleted p21-activated kinase 3 (pak3), which encodes a member of the Pak family of actin-regulatory enzymes, but whose in vivo function is unknown. We show that pak3 mutants have only mild synaptic defects at the larval neuromuscular junction, but exhibit a potent genetic interaction with spastin mutations. Aberrant bouton morphology, microtubule distribution, and synaptic transmission caused by spastin loss of function are all restored to wild type when pak3 is simultaneously reduced. Neuronal overexpression of pak3 induces actin-rich thin projections, suggesting that it functions in vivo to promote filopodia during presynaptic terminal arborization. pak3 therefore regulates synapse development in vivo, and when mutated, suppresses the synaptic defects that result from spastin loss.
journal_name
Geneticsjournal_title
Geneticsauthors
Ozdowski EF,Gayle S,Bao H,Zhang B,Sherwood NTdoi
10.1534/genetics.111.130831subject
Has Abstractpub_date
2011-09-01 00:00:00pages
123-35issue
1eissn
0016-6731issn
1943-2631pii
genetics.111.130831journal_volume
189pub_type
杂志文章相关文献
GENETICS文献大全abstract::Biological evolution generates a surprising amount of site-specific variability in protein sequences. Yet, attempts at modeling this process have been only moderately successful, and current models based on protein structural metrics explain, at best, 60% of the observed variation. Surprisingly, simple measures of pro...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300699
更新日期:2018-04-01 00:00:00
abstract::Ribosome biogenesis is tightly regulated through stress-sensing pathways that impact genome stability, aging and senescence. In Saccharomyces cerevisiae, ribosomal RNAs are transcribed from rDNA located on the right arm of chromosome XII. Numerous studies reveal that rDNA decondenses into a puff-like structure during ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302994
更新日期:2020-03-01 00:00:00
abstract::The nucleotide sequences encoding the mature portion of 31 ribulose 1.5-bisphosphate carboxylase small subunit (SSU) genes from 17 genera of plants, green algae and cyanobacteria were examined. Among the 465 pairwise sequence comparisons, SSU multigene family members within the same species were more similar to each o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Transcription is repressed in regions of the fission yeast genome close to centromeres, telomeres, or the silent mating-type cassettes mat2-P and mat3-M. The repression involves the chromo-domain proteins Swi6 and Clr4. We report that two other chromo-domain proteins, Chp1 and Chp2, are also important for these positi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::A lethal locus (l(2)br7;35B6-10), near Adh on chromosome arm 2L of D. melanogaster, is identified with Plunkett's dominant suppressor of Hairless (H). Of eight new alleles, seven act as dominant suppressors of H, the eighth is a dominant enhancer of H. One of the suppressor alleles is both a leaky lethal and a weak s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::In fission yeast, the septation initiation network (SIN) is thought to promote cytokinesis by downstream activation of Rho1, a conserved GTPase that controls cell growth and division. Here we show that Etd1 and PP2A-Pab1, antagonistic regulators of SIN, are Rho1 regulators. Our genetic and biochemical studies indicate...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.155218
更新日期:2014-02-01 00:00:00
abstract::Fossil evidence links human ancestry with populations that evolved from modern gracile morphology in Africa 130,000-160,000 years ago. Yet fossils alone do not provide clear answers to the question of whether the ancestors of all modern Homo sapiens comprised a single African population or an amalgamation of distinct ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.041095
更新日期:2005-08-01 00:00:00
abstract::Self-incompatibility (SI) is a genetic system found in some hermaphrodite plants. Recognition of pollen by pistils expressing cognate specificities at two linked genes leads to rejection of self pollen and pollen from close relatives, i.e., to avoidance of self-fertilization and inbred matings, and thus increased outc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.127399
更新日期:2011-07-01 00:00:00
abstract::Specific mutations in the gene encoding the largest subunit of RNA polymerase II (RpII215) cause a partial transformation of a structure of the third thoracic segment, the capitellum, into the analogous structure of the second thoracic segment, the wing. This mutant phenotype is also caused by genetically reducing the...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Sexual reproduction and recombination are important for maintaining a stable copy number of transposable elements (TEs). In sexual populations, elements can be contained by purifying selection against host carriers with higher element copy numbers; however, in the absence of sex and recombination, asexual populations ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.060434
更新日期:2006-10-01 00:00:00
abstract::Transposable elements (TEs) constitute a substantial fraction of the eukaryotic genome and, as a result, have a complex relationship with their host that is both adversarial and dependent. To minimize damage to cellular genes, TEs possess mechanisms that target integration to sequences of low importance. However, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.181602
更新日期:2015-11-01 00:00:00
abstract::This FlyBook chapter summarizes the history and the current state of our understanding of the Wingless signaling pathway. Wingless, the fly homolog of the mammalian Wnt oncoproteins, plays a central role in pattern generation during development. Much of what we know about the pathway was learned from genetic and molec...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.117.300157
更新日期:2018-04-01 00:00:00
abstract::We have isolated 136 independent mutations in haploid yeast strains that exhibit decreased chromosome transmission fidelity in mitosis. Eighty-five percent of the mutations are recessive and 15% are partially dominant. Complementation analysis between MATa and MAT alpha isolates identifies 11 chromosome transmission f...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Gene targeting techniques have led to the phenotypic characterization of numerous genes; however, many genes show minimal to no phenotypic consequences when disrupted, despite many having highly conserved sequences. The standard explanation for these findings is functional redundancy. A competing hypothesis is that th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.178079
更新日期:2015-10-01 00:00:00
abstract::I factors in Drosophila melanogaster are non-LTR retrotransposons that transpose at very high frequencies in the germ line of females resulting from crosses between reactive females (devoid of active I factors) and inducer males (containing active I factors). Constructs containing I factor ORF1 under the control of th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Germ cell specification as sperm or oocyte is an ancient cell fate decision, but its molecular regulation is poorly understood. In Caenorhabditis elegans, the FOG-1 and FOG-3 proteins behave genetically as terminal regulators of sperm fate specification. Both are homologous to well-established RNA regulators, suggesti...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.182592
更新日期:2016-01-01 00:00:00
abstract::Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198812
更新日期:2017-07-01 00:00:00
abstract::Evidence from many organisms indicates that the conserved RecQ helicases function in the maintenance of genomic stability. Mutation of SGS1 and WRN, which encode RecQ homologues in budding yeast and humans, respectively, results in phenotypes characteristic of premature aging. Mutation of SRS2, another DNA helicase, c...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::The MITF protein is a member of the MYC family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors and is most closely related to the TFE3, TFEC, and TFEB proteins. In the mouse, MITF is required for the development of several different cell types, including the retinal pigment epithelial (RPE) c...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.167.1.233
更新日期:2004-05-01 00:00:00
abstract::Previous models suggested that regulation of telomere length in Saccharomyces cerevisiae by Tel1(ATM) and Mec1(ATR) would parallel the established pathways regulating the DNA damage response. Here, we provide evidence that telomere length regulation differs from the DNA damage response in both the Tel1 and Mec1 pathwa...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302713
更新日期:2019-12-01 00:00:00
abstract::Meiotically driven sex chromosomes can quickly spread to fixation and cause population extinction unless balanced by selection or suppressed by genetic modifiers. We report results of genetic analyses that demonstrate that extreme female-biased sex ratios in two sister species of stalk-eyed flies, Cyrtodiopsis dalmann...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Yeast mitochondria form a branched tubular network. Mitochondrial inheritance is tightly coupled with bud emergence, ensuring that daughter cells receive mitochondria from mother cells during division. Proteins reported to influence mitochondrial inheritance include the mitochondrial rho (Miro) GTPase Gem1p, Mmr1p, an...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083055
更新日期:2008-02-01 00:00:00
abstract::Tetrahymena is a useful eukaryotic model for biochemistry and molecular cell biology studies. We previously demonstrated that targeted ectopic DNA elimination, also called co-Deletion (coDel), can be induced by the introduction of an internal eliminated sequence (IES)-target DNA chimeric construct. In this study, we d...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.178525
更新日期:2015-09-01 00:00:00
abstract::Antisuppressor mutations in the eEF1A gene of Podospora anserina were previously shown to impair ascospore formation, to drastically increase life span, and to permit the development of the Crippled Growth degenerative process. Here, we show that eEF1A controls ascospore formation through accuracy level maintenance. E...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::The bovine Growth Hormone gene (bGH) is an attractive candidate gene for milk production in cattle. Single-strand conformation polymorphisms at bGH were identified and used to define haplotype configurations at this gene in the Israeli Holstein dairy cattle population (Bos taurus) and in the parent animals of the Inte...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitos from 13 backcross families that included 679 progeny....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::The POL3 encoded catalytic subunit of DNA polymerase delta possesses a highly conserved C-terminal cysteine-rich domain in Saccharomyces cerevisiae. Mutations in some of its cysteine codons display a lethal phenotype, which demonstrates an essential function of this domain. The thermosensitive mutant pol3-13, in which...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Serrate (Ser) is an essential gene in Drosophila melanogaster best known for the Ser dominant (SerD) allele and its effects on wing development. Animals heterozygous or homozygous for SerD are viable and exhibit loss of wing margin tissue and associated bristles and hairs. The Beaded of Goldschmidt (BdG) allele of Ser...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Significant correlations between allelic frequencies and environmental variables in a number of insect species have been demonstrated by multivariate techniques. Since many environmental variables show a strong relationship to geographic location and since gene flow between populations can also produce patterns of gen...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-05-01 00:00:00
abstract::Disequilibrium pattern analysis, a general method for analyzing evolutionary events acting on pairs of tightly linked polymorphic loci, is applied to a large sample of Danish individuals typed for A and B loci of the HLA (human leukocyte antigen) system. Cases of selection on particular haplotypes are revealed from pa...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00