Abstract:
:The needs for purified nucleic acids for preparative and analytical applications have increased constantly, demanding for the development of new and more efficient methods for their recovery and isolation. DNA molecules harbour some intrinsic chemical properties that render them suitable for chromatographic separations. These include a negatively charged phosphate backbone as well as a hydrophobic character originating mainly from the major groove of DNA which exposes the base pairs on the surface of the molecule. In addition, single stranded DNA often allows for a free exposure of the hydrophobic aromatic bases. In this review, multimodal chromatography (MMC) has been evaluated as an alternative tool for complex separations of nucleic acids. MMC embraces more than one kind of interaction between the chromatographic ligand and the target molecules. These resins have often proved superior to conventional single-mode chromatographic materials for DNA isolation, including, e.g., the purification of plasmid DNA from crude cell lysates and for the preparation of DNA fragments before or after a polymerase chain reaction (PCR).
journal_name
Curr Protein Pept Scijournal_title
Current protein & peptide scienceauthors
Matos T,Bülow Ldoi
10.2174/1389203718666171024112556subject
Has Abstractpub_date
2019-01-01 00:00:00pages
49-55issue
1eissn
1389-2037issn
1875-5550pii
CPPS-EPUB-86492journal_volume
20pub_type
杂志文章,评审abstract::The number of protein folds in nature is limited, thus is not surprising that proteins with the same fold are able to exert different functions. The cysteine protease inhibitors that adopt an immunoglobulin- like fold (Ig-ICPs) are inhibitors encoded in bacteria and protozoan parasites. Structural studies indicate tha...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666160813163837
更新日期:2017-01-01 00:00:00
abstract::Receptor tyrosine kinases (RTKs) are cell surface receptors that transmit extracellular signals to the interior of the cell activating multiple signaling cascades: the binding of specific extracellular ligands activates RTKs which act as a scaffold at plasma membrane level for the recruitment and activation of several...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/13892037113146660087
更新日期:2013-11-01 00:00:00
abstract::For several decades the specificity of proteases has been presented as an active site divided into subsites, using the nomenclature of Schechter & Berger from 1967 (S1, S2... for subsites of the active site; P1, P2... for residues of the substrate occupying the corresponding subsites). At early stages of the research ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920305774933286
更新日期:2005-12-01 00:00:00
abstract::Significant differences in function have been observed among lectins structurally similar to concanavalin A, but their high homology with this widely used lectin has kept them in obscurity. The observation of large differences in the potency of many of these Diocleinae lectins as stimulators of Interferon-g production...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203013381152
更新日期:2001-06-01 00:00:00
abstract::Hemoglobin (Hb) is the prototypical example of a cooperative protein. Cooperativity of Hb is largely accounted for by the oxygen-linked allosteric interconversion between the T and R states/structures. Allostery is such a powerful explanation of Hb cooperativity that the possibility of cooperative events occurring wit...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203718666171030103310
更新日期:2018-01-01 00:00:00
abstract::CD2 (cluster of differentiation 2) is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). Tremendous progress has been achieved in understanding the function of CD2, the mechanism of molecular recognition and protein folding, thus, leading towards the use of t...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203033487063
更新日期:2003-10-01 00:00:00
abstract::Structure based drug designing is now a popular technique used for increasing the speed of drug designing process. This was made possible by the availability of many protein structures which helped in developing tools to understand the structure function relationships, automated docking and virtual screening. Knowledg...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920307781369454
更新日期:2007-08-01 00:00:00
abstract::ATPases are important molecular machines that convert the chemical energies stored in ATP to mechanical actions within the cell. ATPases are among the most abundant proteins with diverse functions involved in almost every cellular pathway. The well characterised ATPases include the various motor proteins responsible f...
journal_title:Current protein & peptide science
pub_type: 杂志文章
doi:10.2174/1389203043486874
更新日期:2004-04-01 00:00:00
abstract::Carnosine (β-alanyl-L-histidine) and its methylated derivatives: anserine (β-alanyl-Nπ- methyl-L-histidine) and balenine (β-alanyl-Nτ-methyl-L-histidine) are abundant constituents of excitable tissues of vertebrates. While carnosine and anserine are present at high concentrations and in variable proportions in skeleta...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203719666180226155657
更新日期:2018-01-01 00:00:00
abstract::Melittin, a major component of bee venom, is a water-soluble toxic peptide of which a various biological effects have been identified to be useful in anti-tumor therapy. In addition, Melittin also has anti-parasitic, anti-bacterial, anti-viral, and anti-inflammatory activities. Therefore, it is a very attractive thera...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203719666180612084615
更新日期:2019-01-01 00:00:00
abstract::Parvalbumin (PA) is a classical small, mostly cytosolic Ca2+-binding protein of the EF-hand superfamily expressed in vertebrates in a tissue- and cell-specific manner, serving as a magnesium/ calcium buffer. In the last decade novel data were published on structural peculiarities of PA, likely affecting its functional...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666161213115746
更新日期:2017-01-01 00:00:00
abstract::The aggregation of proteins or their digested fragments through β-sheet structures has a great significance because it leads to neurodegenerative diseases, which are a problem of the aging societies of the developed countries. Short peptides are typically used as models to study the formation of specific structures. H...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203718666170516111601
更新日期:2017-01-01 00:00:00
abstract::The family of peptides designated as cathelicidins was identified over a decade ago. Cathelicidins have since gained increasing recognition, both as endogenous antibiotics and as effector molecules of the innate immune system. The human cathelicidin LL-37 is widely expressed in human tissues and plays diverse biologic...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/13892037113149990067
更新日期:2013-09-01 00:00:00
abstract::Pathogenic aggregation is closely associated with various protein misfolding diseases such as type 2 diabetes mellitus and Alzheimer's disease. Amyloidogenic proteins that have a propensity to assemble into amyloid oligomers and fibrils form the aggregates. The tumor suppressor p53, a transcription factor that regulat...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:
更新日期:2015-01-01 00:00:00
abstract::TOYOPEARL particles are cross-linked hydroxylated methacrylic polymers available in different pore and particle sizes. They are conjugated with different ligands to generate ion-exchange, hydrophobic interaction and affinity resins. They have excellent physical and chemical properties. A mixed-mode resin, TOYOPEARL MX...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203718666171009111355
更新日期:2019-01-01 00:00:00
abstract::The current lack of complete understanding of the pathogenesis of acute kidney injury (AKI) is a significant barrier to its early diagnosis and treatment. Cell cycle arrest plays an important role in the protection of renal tubular epithelial cells and maladaptive repair following AKI. G1 phase cell arrest serves as a...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666160915162238
更新日期:2017-01-01 00:00:00
abstract::Elevated blood pressure or hypertension is one of the fastest growing health problems worldwide. Although the etiology of essential hypertension has a genetic component, dietary factors play an important role. With the high costs and adverse side-effects associated with synthetic antihypertensive drugs and the awarene...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/13892037113149990036
更新日期:2013-05-01 00:00:00
abstract::Many naturally occurring bioactive peptides bind to biological membranes. Studying and elucidating the mode of interaction is often an essential step to understand their molecular and biological functions. To obtain the complete orientation and immersion depth of such compounds in the membrane or a membrane-mimetic sy...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312800785049
更新日期:2012-05-01 00:00:00
abstract::The bioactive peptides are protein fragments which have a positive impact on the intestinal homeostasis. Intestinal homeostasis depends on the diverse functions of intestinal barrier including the microbiological, physical, chemical and immunological barriers. Defects in intestinal barrier function are associated with...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203716666150630134804
更新日期:2015-01-01 00:00:00
abstract::The Mastermind-like (MAML) proteins are transcriptional coactivators for Notch signaling, an evolutionarily conserved pathway that plays several key roles in development and in human disease. The MAML family contains MAML1, MAML2, and MAML3. More recently, MAML1 has been shown to function as a coactivator for the tumo...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920309789630543
更新日期:2009-12-01 00:00:00
abstract::Host cell entry by influenza and other enveloped viruses is well characterized, however, the manner in which non-enveloped viruses deliver their genome across host cell membranes in the absence of membrane fusion remains unresolved. The discovery of short, membrane altering, amphipathic or hydrophobic sequences in sev...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920308783565732
更新日期:2008-02-01 00:00:00
abstract::The biotin carboxylase family is comprised of a group of enzymes that utilize a covalently bound prosthetic group, biotin, as a cofactor. These enzymes, which include acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, geranoyl-CoA carboxylase, oxaloacetate decarbox...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203033487199
更新日期:2003-06-01 00:00:00
abstract::Allergen-specific Immunotherapy (AIT), through repetitive subcutaneous or sublingual administrations of allergen extracts, represents up to now the unique treatment against allergic sensitizations. However, the clinical efficacy of AIT can be largely dependent on the quality of natural allergen extracts. Moreover, the...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190718152534
更新日期:2020-01-01 00:00:00
abstract::The absence of uracil from DNA genomes is a consequence of enzyme functions that eliminate intracellular dUTP pools and that purposefully recognize and remove uracil moieties from DNA. These enzymatic functions are dUTP nucleotidohydrolase (dUTPase) and uracil-DNA glycosylase (UDG), respectively. There are distinct nu...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203013381044
更新日期:2001-12-01 00:00:00
abstract::To defend themselves from attack by pathogens, plants can rely only on their innate defense systems. Defensins are antimicrobial peptides that contribute to plant immunity by displaying a direct cidal activity against various pathogens, some of which are responsible for plant diseases. These determine a significant de...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920310791112101
更新日期:2010-05-01 00:00:00
abstract::The review presents three hypotheses concerning the amino acid complementarity: 1) the Mekler-Blalock antisense hypothesis; 2) the Root-Bernstein approach based on stereochemical complementarity of amino acids and anti-amino acids coded by anticodons read in parallel with the coding DNA strand; 3) Siemion hypothesis r...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203043379413
更新日期:2004-12-01 00:00:00
abstract::Here we review the present state of structural and functional studies of the Sac10b protein family, a class of highly conserved 10 kDa nucleic acid-binding proteins in archaea. Based on biochemical and structural studies, these proteins were originally assigned a role in the structural organization of chromatin; Sac10...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312800785067
更新日期:2012-05-01 00:00:00
abstract::Serine protease inhibitors (PIs) are a large and complex group of plant proteins. Members of the potato type I (Pin1) and II (Pin2) proteinase inhibitor families are among the first and most extensively characterized plant PIs. Many insects and phytopathogenic microorganisms use intracellular and extracellular serine ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920311796391151
更新日期:2011-08-01 00:00:00
abstract::Fibroids or myomas involve large proportion of women of reproductive age. The myoma formation starts from the transformation of the myometrium, causing the progressive formation of a pseudocapsule, which is made of compressed muscle fibers. Numerous studies investigated on myoma pseudocapsule anatomy, discovering many...
journal_title:Current protein & peptide science
pub_type: 杂志文章
doi:10.2174/1389203717666160322150338
更新日期:2017-01-01 00:00:00
abstract::Covalent conjugation of the ubiquitin tag to cellular proteins plays a central role in a number of processes, the most notable among them being degradation by the 26S proteasome. A fundamental property of this process is that ubiquitination, in contrast to subsequent degradation, is reversible due to a number of deubi...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203043379756
更新日期:2004-06-01 00:00:00