Abstract:
:Hemoglobin (Hb) is the prototypical example of a cooperative protein. Cooperativity of Hb is largely accounted for by the oxygen-linked allosteric interconversion between the T and R states/structures. Allostery is such a powerful explanation of Hb cooperativity that the possibility of cooperative events occurring within each allosteric conformation, in the absence of any quaternary structural change has usually been overlooked, and actually experiments specifically aimed at detecting nonallosteric cooperativity have usually failed to do so. However there are strong, but often neglected, theoretical reasons pointing to the presence of nonallosteric cooperativity under common experimental conditions, that have recently raised new interest and have been thoroughly re-investigated. Non-allosteric cooperativity within T state Hb has often been invoked to describe puzzling experimental data, either as an intrinsic property of the macromolecule or as a consequence of the binding of non-heme ligands. Few convincing pieces of evidence exist for the former hypothesis, whereas very strong proofs are available for effector-induced non-allosteric cooperativity in hemoglobin. Moreover, non-allosteric cooperativity in THb may explain some hitherto puzzling findings, e.g. the bi-exponential O2 release from THb observed by Q.H. Gibson in oxygen pulse experiments, the invariance of L4 found by K. Imai, the cooperative ligand binding by crystals of T state Hb Rotschild, and, possibly, the cooperativity observed in at least some mixed metal hybrid Hbs.
journal_name
Curr Protein Pept Scijournal_title
Current protein & peptide scienceauthors
Bellelli Adoi
10.2174/1389203718666171030103310subject
Has Abstractpub_date
2018-01-01 00:00:00pages
573-588issue
6eissn
1389-2037issn
1875-5550pii
CPPS-EPUB-86571journal_volume
19pub_type
杂志文章,评审abstract::Structure based drug designing is now a popular technique used for increasing the speed of drug designing process. This was made possible by the availability of many protein structures which helped in developing tools to understand the structure function relationships, automated docking and virtual screening. Knowledg...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920307781369454
更新日期:2007-08-01 00:00:00
abstract::The field of bioinformatics has become a major part of the drug discovery pipeline playing a key role in improvement and acceleration of this time and money consuming process. Here we review the application of the informational spectrum method (ISM), a virtual spectroscopy method for structure/function analysis of pro...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920308785915245
更新日期:2008-10-01 00:00:00
abstract::Poly-N-acetyllactosamine structures occur in mammalian glycoproteins in both N- and O-linked glycans. They represent a backbone for additional modifications by fucosyltransferases, sialyltransferases and sulfotransferases. These glycans have been suggested to be involved in biospecific interactions with selectins and ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203033380304
更新日期:2003-02-01 00:00:00
abstract::The cytolysin is a novel, two-peptide lytic toxin produced by some strains of Enterococcus faecalis. It is toxic in animal models of enterococcal infection, and associated with acutely terminal outcome in human infection. The cytolysin exerts activity against a broad spectrum of cell types including a wide range of gr...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203053027557
更新日期:2005-02-01 00:00:00
abstract::Calmodulin (CaM) is a highly conserved eukaryotic Ca2+ sensor protein that is able to bind a large variety of target sequences without a defined consensus sequence. The recognition of this diverse target set allows CaM to take part in the regulation of several vital cell functions. To fully understand the structural b...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190925101937
更新日期:2019-01-01 00:00:00
abstract::Lactoferrin or lactotransferrin is a multifunctional glycoprotein found in blood circulation, mucosal surfaces, neutrophils, and in various secretory fluids, such as milk, bile, tears, nasal secretion, pancreatic juice, and saliva. The lactoferrin content in milk varies between different mammalian species and, within ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203715666140919124530
更新日期:2014-01-01 00:00:00
abstract::Bacteria carry a battery of multidrug transporters, which belong to six families of transporters. Members of at least three families the ATP-Binding Cassette superfamily, the Major Facilitator Superfamily and the Multidrug Endosomal Transporter family have been shown to contribute to multidrug resistance phenotype in ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203023380512
更新日期:2002-10-01 00:00:00
abstract::Arginine is widely used in such applications as protein refolding, solubilization of proteins and small molecules, protein and small molecule formulation, column chromatography and viral inactivation as summarized in this review. What makes arginine effective in these applications is largely based on its ability to su...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920371506140818113015
更新日期:2014-01-01 00:00:00
abstract::The majority of tRNA studies has focused on tRNA molecules as pivotal player in the fundamental process of protein synthesis. Mounting studies have unveiled further functions for tRNA beyond protein synthesis, including non-ribosomal amino acid transfer, and regulation of targeted proteolysis. Post-translational N-ter...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203719666180809113122
更新日期:2018-01-01 00:00:00
abstract::The current lack of complete understanding of the pathogenesis of acute kidney injury (AKI) is a significant barrier to its early diagnosis and treatment. Cell cycle arrest plays an important role in the protection of renal tubular epithelial cells and maladaptive repair following AKI. G1 phase cell arrest serves as a...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666160915162238
更新日期:2017-01-01 00:00:00
abstract::While the crucial role of intrinsically disordered proteins (IDPs) in the cell cycle is now recognized, deciphering their molecular mode of action at the structural level still remains highly challenging and requires a combination of many biophysical approaches. Among them, small angle X-ray scattering (SAXS) has been...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312799277901
更新日期:2012-02-01 00:00:00
abstract::Membrane proteins play important roles in signal transduction across the cell membrane. Structural information for the membrane proteins is still limited due to many technical challenges. Membrane proteins containing a single α- helical transmembrane (TM) domain are very important in several pathways. Solution NMR spe...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312803582979
更新日期:2012-09-01 00:00:00
abstract::Dietary protein from fermented cottonseed meal (FCSM), widely used in poultry diets in China, had regulating effects on lipid metabolism. To understand the effects of FCSM on lipid metabolism in broilers, we analyzed the biochemical indexes, enzyme activity, hormone level and metabolites in serum responses to FCSM int...
journal_title:Current protein & peptide science
pub_type: 杂志文章
doi:10.2174/1389203721666200203152643
更新日期:2020-01-01 00:00:00
abstract::Porphyromonas gingivalis is a Gram-negative anaerobic bacterium that is implicated as a major etiologic agent of adult periodontal disease. This bacterium is asaccharolytic and possesses strong potency for proteolysis. It produces a novel class of cysteine proteinases, termed gingipains, in the cell-associated and sec...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203033486992
更新日期:2003-12-01 00:00:00
abstract::This review is to summarize three new QSAR (quantitative structure-activity relationship) methods recently developed in our group and their applications for drug design. Based on more solid theoretical models and advanced mathematical techniques, the conventional QSAR technique has been recast in the following three a...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920308784534005
更新日期:2008-06-01 00:00:00
abstract::Here we review the present state of structural and functional studies of the Sac10b protein family, a class of highly conserved 10 kDa nucleic acid-binding proteins in archaea. Based on biochemical and structural studies, these proteins were originally assigned a role in the structural organization of chromatin; Sac10...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312800785067
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Drug-Target Interactions (DTI) play a crucial role in discovering new drug candidates and finding new proteins to target for drug development. Although the number of detected DTI obtained by high-throughput techniques has been increasing, the number of known DTI is still limited. On the other hand, the exper...
journal_title:Current protein & peptide science
pub_type: 杂志文章
doi:10.2174/1389203718666161122103057
更新日期:2018-01-01 00:00:00
abstract::Mites and ticks are associated with many human diseases including allergic diseases and scabies. With the recent advances in the high throughput DNA sequencing technology, many mitochondrial nuclear genomes of these species have been sequenced and the resulting genomic resources will certainly provide novel insights f...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190719150432
更新日期:2020-01-01 00:00:00
abstract::Proteasomes are the major nonlysosomal protein degradation machinery in eukaryotic cells and they are largely responsible for the processing of antigens for presentation by the MHC class I pathway. This review concentrates on recent developments in the area of antigen processing. Specialized proteasomes called immunop...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203043379774
更新日期:2004-06-01 00:00:00
abstract::Fibroids or myomas involve large proportion of women of reproductive age. The myoma formation starts from the transformation of the myometrium, causing the progressive formation of a pseudocapsule, which is made of compressed muscle fibers. Numerous studies investigated on myoma pseudocapsule anatomy, discovering many...
journal_title:Current protein & peptide science
pub_type: 杂志文章
doi:10.2174/1389203717666160322150338
更新日期:2017-01-01 00:00:00
abstract::Identifying the interactions between drugs and target proteins is a key step in drug discovery. This not only aids to understand the disease mechanism, but also helps to identify unexpected therapeutic activity or adverse side effects of drugs. Hence, drug-target interaction prediction becomes an essential tool in the...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203718666161108091609
更新日期:2018-01-01 00:00:00
abstract::Toll-like receptors (TLRs) are type I transmembrane signaling molecules that are expressed in cells of the innate immune system. In these cells, TLRs function as pattern recognition receptors (PRR) that recognize specific molecular patterns derived from microorganisms. Upon activation, TLRs trigger a cascade of intrac...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203711314010006
更新日期:2013-02-01 00:00:00
abstract::Serine protease inhibitors (PIs) are a large and complex group of plant proteins. Members of the potato type I (Pin1) and II (Pin2) proteinase inhibitor families are among the first and most extensively characterized plant PIs. Many insects and phytopathogenic microorganisms use intracellular and extracellular serine ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920311796391151
更新日期:2011-08-01 00:00:00
abstract::Glycoconjugates comprise a variety of structures, include glycoproteins and glycolipids and are found on the surfaces of animal and plant cells, as well as on the surface of microorganisms. Determination of the structure and the distribution of glycoconjugates on cell surfaces are important for the understanding their...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920307780831811
更新日期:2007-06-01 00:00:00
abstract::The number of protein folds in nature is limited, thus is not surprising that proteins with the same fold are able to exert different functions. The cysteine protease inhibitors that adopt an immunoglobulin- like fold (Ig-ICPs) are inhibitors encoded in bacteria and protozoan parasites. Structural studies indicate tha...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666160813163837
更新日期:2017-01-01 00:00:00
abstract::Parvalbumin (PA) is a classical small, mostly cytosolic Ca2+-binding protein of the EF-hand superfamily expressed in vertebrates in a tissue- and cell-specific manner, serving as a magnesium/ calcium buffer. In the last decade novel data were published on structural peculiarities of PA, likely affecting its functional...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666161213115746
更新日期:2017-01-01 00:00:00
abstract::Skeletal muscle, the main protein reservoir in the body, is a tissue that exhibits high plasticity when exposed to changes. Muscle proteins can be mobilized into free amino acids when skeletal muscle wasting occurs, a process called skeletal muscle atrophy. This wasting is an important systemic or local manifestation ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312803582933
更新日期:2012-09-01 00:00:00
abstract::As global resistance to conventional antibiotics rises we need to develop new strategies to develop future novel therapeutics. In our quest to design novel anti-infectives and antimicrobials it is of interest to investigate host-pathogen interactions and learn from the complexity of host defense strategies that have e...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312804871139
更新日期:2012-12-01 00:00:00
abstract::Significant differences in function have been observed among lectins structurally similar to concanavalin A, but their high homology with this widely used lectin has kept them in obscurity. The observation of large differences in the potency of many of these Diocleinae lectins as stimulators of Interferon-g production...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203013381152
更新日期:2001-06-01 00:00:00
abstract::Efforts to use computers in predicting the secondary structure of proteins based only on primary structure information started over a quarter century ago [1-3]. Although the results were encouraging initially, the accuracy of the pioneering methods generally did not attain the level required for using predictions of s...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203003381324
更新日期:2000-11-01 00:00:00