Abstract：
:miRNAs have been implicated in processing of cardiac hypoxia/reoxygenation (H/R)-induced injury. Recent studies demonstrated that miR-19a might provide a potential cardioprotective effect on myocardial disease. However, the effect of miR-19a in regulating myocardial ischemic injury has not been previously addressed. The present study was to investigate the effect of miR-19a on myocardial ischemic injury and identified the potential molecular mechanisms involved. Using the H/R model of rat cardiomyocytes H9C2 in vitro, we found that miR-19a was in low expression in H9C2 cells after H/R treatment and H/R dramatically decreased cardiomyocyte viability, and increased lactate dehydrogenase (LDH) release and cardiomyocyte apoptosis, which were attenuated by co-transfection with miR-19a mimic. Dual-luciferase reporter assay and Western blotting assay revealed that PTEN was a direct target gene of miR-19a, and miR-19a suppressed the expression of PTEN via binding to its 3'-UTR. We further identified that overexpression of miR-19a inhibited the expression of PTEN at the mRNA and protein levels. Moreover, PTEN was highly expressed in H/R H9C2 cells and the apoptosis induced by H/R was associated with the increase in PTEN expression. Importantly, miR-19a mimic significantly increased p-Akt levels under H/R. In conclusion, our findings indicate that miR-19a could protect against H/R-induced cardiomyocyte apoptosis by inhibiting PTEN /PI3K/p-Akt signaling pathway.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Sun G,Lu Y,Li Y,Mao J,Zhang J,Jin Y,Li Y,Sun Y,Liu L,Li Ldoi
10.1042/BSR20170899subject
Has Abstractpub_date
2017-12-05 00:00:00issue
6eissn
0144-8463issn
1573-4935pii
BSR20170899journal_volume
37pub_type
杂志文章abstract：:Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. Due to sensitivity of biological...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170203
更新日期：2018-04-27 00:00:00
abstract：:Secretion of high molecular weight (HMW) adiponectin is dependent on post-translational modification (PTM) of conserved lysines in the collagenous domain. The present study aims to characterize the enzymes responsible for the PTM of conserved lysines which leads to HMW adiponectin secretion, and to define its signific...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170105
更新日期：2017-05-17 00:00:00
abstract：:This review focuses on the recent developments in the study of drug interactions with biological membranes and membrane-associated proteins using nuclear magnetic resonance (NMR) spectroscopy and other spectroscopic techniques. Emphasis is placed on a class of low-affinity neurological agents as exemplified by volatil...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1023/a:1020182404940
更新日期：2002-04-01 00:00:00
abstract：:FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural simila...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20194431
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abstract：:Blood pressure (BP) is a known prognostic marker for mortality in patients on maintenance hemodialysis (MHD). However, definition of the BP and its optimal values vary essentially among different MHD populations. Our purpose was to clarify these important clinical parameters in a Chinese MHD cohort. Accordingly, we re...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200858
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abstract：:Gallbladder cancer (GBC) is a relatively rare but fatal gastrointestinal tumor. The microRNA-33b (miR-33b), a member of miR-33 family, is reported to function as a tumor suppressor in various cancers. Notably, miR-33 was predicted to target CROCC based on microarray-based analysis. Hereby, we aimed to characterize the...
journal_title：Bioscience reports
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1042/BSR20171234
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01182482
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journal_title：Bioscience reports
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doi：10.1042/BSR20170301
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119197
更新日期：1991-04-01 00:00:00
abstract：:To determine whether up-regulation of miR-1183 targeting the gene for anti-apoptotic factor, B-cell lymphoma 2 (BCL-2) contributes to apoptosis in patients with rheumatic heart disease (RHD). Peripheral blood samples were isolated for miR-1183 characterization. The function of miRNA-1183 in RHD using miRNA mimic on PB...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201573
更新日期：2020-11-27 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01118600
更新日期：1991-02-01 00:00:00
abstract：:TRα1 (thyroid hormone receptor α1) is well recognized for its importance in brain development. However, due to the difficulties in predicting TREs (thyroid hormone response elements) in silico and the lack of suitable antibodies against TRα1 for ChIP (chromatin immunoprecipitation), only a few direct TRα1 target genes...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20120124
更新日期：2013-03-13 00:00:00
abstract：:Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114868
更新日期：1981-04-01 00:00:00
abstract：:A few studies suggested the contribution of PPARs to the etiology of schizophrenia (SCZ). However, it is still not clear whether variants in PPAR-encoding genes have a direct association with SCZ. The potential linkage between SCZ and the variants within PPAR encoding genes (PPARA, PPARD, and PPARG) was tested in a la...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201083
更新日期：2020-07-31 00:00:00
abstract：:Endothelin-1 (ET-1) and plasminogen activator inhibitor-1 (PAI-1) play important roles in pulmonary hypertension (PH) in sickle cell disease (SCD). Our previous studies show higher levels of placenta growth factor (PlGF) in SCD correlate with increased plasma levels of ET-1, PAI-1, and other physiological markers of P...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20150190
更新日期：2015-10-12 00:00:00
abstract：:Chitinases catalyze the degradation of chitin, a ubiquitous polymer generated from the cell walls of fungi, shells of crustaceans, and cuticles of insects. They are gaining increasing attention in medicine, agriculture, food and drug industries, and environmental management. Their roles in the degradation of chitin fo...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1042/BSR20180323
更新日期：2018-08-29 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章,收录出版
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20171082
更新日期：2017-11-15 00:00:00
abstract：:The inhibition of passive K+ influx into human red blood cells (RBC) by loop diuretics was found to be dependent on the external Na+ concentration. In the absence of external Na+, there was minimal inhibition but the influx remained dependent on Cl- ions. Thus, raising the external Na+ concentration increased the affi...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116900
更新日期：1985-04-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1013290316939
更新日期：2001-06-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116377
更新日期：1982-03-01 00:00:00
abstract：OBJECTIVES:To investigate the association between dectin-1 gene single nucleotide polymorphisms (SNPs) and susceptibility to fungal infection (FI). METHODS:Databases were searched electronically and manually to identify case-control studies concerning dectin-1 SNPs and FI, which were published up to 12 November 2018. ...
journal_title：Bioscience reports
pub_type： 杂志文章,meta分析
doi：10.1042/BSR20191519
更新日期：2019-11-29 00:00:00
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journal_title：Bioscience reports
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doi：10.1042/BSR20180367
更新日期：2018-05-22 00:00:00
abstract：:Background: Single-nucleotide polymorphisms (SNPs) in lncRNAs could be biomarkers for susceptibility to colorectal cancer (CRC), but the association of PCAT1 polymorphisms and CRC susceptibility is yet to be studied. Methods: Five tagSNPs covering the PCAT1 gene were detected through Kompetitive Allele-Specific PCR am...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190708
更新日期：2019-07-12 00:00:00
abstract：:MicroRNAs (miRNAs) have been frequently identified as key mediators in almost all developmental and pathological processes, including those in the liver. The present study was conducted with aims of investigating the role of microRNA-138 (miR-138) in acute liver failure (ALF) via a mechanism involving p53 and liver ma...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190763
更新日期：2019-07-18 00:00:00
abstract：:Background: Endometrial carcinoma (EC) still threatens the health of women. Thus, to explore how long intergenic non-protein coding RNA 01220 regulates the development of EC.Methods: Whole genome expression profile data of EC and paracancerous tissues in TCGA database were downloaded. LINC01220 expression in EC and pa...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181794
更新日期：2019-07-25 00:00:00
abstract：:Two mouse monoclonal antibodies against the human complement control protein, Factor H (beta 1H), are described. The antibodies are both IgG - gamma 1 - subclass and are directed against different epitopes on the human Factor H molecule. One of the antibodies, MRC OX 24, increases the cofactor activity of Factor H in ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01120205
更新日期：1983-12-01 00:00:00
abstract：:Gamma- and delta-isomers of hexachlorocyclohexane caused marked decreases in the levels of radioactive phospholipids, and increases in the levels of [3H]arachidonate incorporated into free fatty acids in rat renal tubular cells. The increased radioactivity of free fatty acids arises from the decrease of [3H]arachidona...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01540453
更新日期：1995-08-01 00:00:00
