Abstract:
:Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. Due to sensitivity of biological materials to the electron beam of the microscope, only relatively low electron doses can be applied during imaging. As a result, the signal arising from the structure of interest is overpowered by noise in the images. To increase the signal-to-noise ratio, different image processing-based strategies that aim at coherent averaging of signal have been devised. In such strategies, images are generally assumed to arise from multiple identical copies of the structure. Prior to averaging, the images must be grouped according to the view of the structure they represent and images representing the same view must be simultaneously aligned relatively to each other. For computational reconstruction of the three-dimensional structure, images must contain different views of the original structure. Structures with multiple symmetry-related substructures are advantageous in averaging approaches because each image provides multiple views of the substructures. However, the symmetry assumption may be valid for only parts of the structure, leading to incoherent averaging of the other parts. Several image processing approaches have been adapted to tackle symmetry-mismatched substructures with increasing success. Such structures are ubiquitous in nature and further computational method development is needed to understanding their biological functions.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Huiskonen JTdoi
10.1042/BSR20170203subject
Has Abstractpub_date
2018-04-27 00:00:00issue
2eissn
0144-8463issn
1573-4935pii
BSR20170203journal_volume
38pub_type
杂志文章abstract::The neurotransmitter, serotonin has emerged as a tumor growth factor and immune response regulator through complex signaling pathways. Yip1 Interacting Factor Homolog B (YIF1B) is a membrane protein involved in serotonin receptor (HTR) membrane trafficking and signal transmission in neuropathy. Participation of YIF1B ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201384
更新日期:2020-07-31 00:00:00
abstract::Rabbit reticulocyte lysates, gel filtered on Sephadex G-25 with or without ATP (or its analogs), were preincubated at 37 degrees C and their subsequent binding to p3A4,3'-[32P]pCp was studied. Lysates filtered without ATP or in the presence of 0.1 mM 8-bromo-ATP, 1,N6-etheno-ATP, or ITP showed a time-dependent decreas...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119625
更新日期:1985-12-01 00:00:00
abstract::Erectile dysfunction (ED) is one of the significant complications of diabetes mellitus (DM), and CASR plays an important role in cellular antiapoptosis and NO production in the vascular endothelium by activating PKC. The present study was aimed to investigate the efficacy of Leech and Centipede Granules (LCG) through ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193845
更新日期:2020-02-28 00:00:00
abstract::Although radioactive iodine (131I) treatment (RIT) is recommended as the preferred option for patients with Graves' disease (GD), the predictive factors for its clinical outcomes are still unclear. In the present study, we aim to investigate the factors influencing the success rate of RIT treatment on primary GD with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191609
更新日期:2020-01-31 00:00:00
abstract::Extracorporeal pulsed electromagnetic field (PEMF) has shown the ability to regenerate tissue by promoting cell proliferation. In the present study, we investigated for the first time whether PEMF treatment could improve the myocardial ischaemia/reperfusion (I/R) injury and uncovered its underlying mechanisms.In our s...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160082
更新日期:2016-12-05 00:00:00
abstract::Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160048
更新日期:2016-05-06 00:00:00
abstract:BACKGROUND:Breast cancer (BC) is the most common malignancy among females worldwide. The tumor microenvironment usually prevents effective lymphocyte activation and infiltration, and suppresses infiltrating effector cells, leading to a failure of the host to reject the tumor. CC chemokines play a significant role in in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202042
更新日期:2020-11-27 00:00:00
abstract::High-affinity binding of 3H-folate in Triton X-100 solubilized membranes of human liver displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. Ultrogel AcA 44 chromatography of solubilized membranes saturated with 3H-folate revealed a major peak of 100 kDa and a ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01182482
更新日期:1991-06-01 00:00:00
abstract::The present study was conducted with the aim being to investigate the effect γ-aminobutyric acid type B (GABAB) receptor activation have on spatial cognitive function and hippocampal neurones found in the rat models of type 2 diabetes mellitus (T2DM). T2DM rat models were then established, randomized, and subsequently...
journal_title:Bioscience reports
pub_type: 杂志文章,收录出版
doi:10.1042/BSR20171184
更新日期:2018-01-19 00:00:00
abstract::The growth arrest special 5 (GAS5), as a research hotspot of long noncoding RNAs (lncRNAs), has been reported to be associated with colorectal cancer (CRC). However, the association between polymorphisms in GAS5 and the risk of CRC was not clear. In the present study, a case-control study in 1078 CRC patients and 1175...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190091
更新日期:2019-04-16 00:00:00
abstract:BACKGROUND:Lung adenocarcinoma (LUAD) is the most frequent histological type of lung cancer, and its incidence has displayed an upward trend in recent years. Nevertheless, little is known regarding effective biomarkers for LUAD. METHODS:The robust rank aggregation method was used to mine differentially expressed genes...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20203973
更新日期:2021-01-29 00:00:00
abstract::PDC (pyruvate dehydrogenase complex) is a multi-enzyme complex comprising an E1 (pyruvate decarboxylase), an E2 (dihydrolipomide acetyltransferase) and an E3 (dihydrolipoamide dehydrogenase). PDC catalyses the decarboxylation of pyruvate and forms acetyl-CoA and NADH. In the human malaria parasite Plasmodium falciparu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140150
更新日期:2015-01-14 00:00:00
abstract::Three genotypically suppressible alleles, a1X4, alcA125, and niaD500, are phenotypically suppressed by aminoglycoside antibiotics. Unsuppressible alleles at these loci are unaffected as are known missense mutations at the yA and gdhA loci. This is consistent with the premise that the suppressible mutations are nonsens...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121566
更新日期:1983-01-01 00:00:00
abstract::The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115252
更新日期:1982-07-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) act as crucial modulators during the development of diverse cancers. Although various types of lncRNAs in prostate cancer (PCa) have been explored, quantities of lncRNAs still wait to be exploited. The present study is to probe the functions and mechanism of lncRNA HOXA cluster antisense...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193287
更新日期:2020-08-28 00:00:00
abstract::The large size of a 1.5-MDa ice-binding adhesin [MpAFP (Marinomonas primoryensis antifreeze protein)] from an Antarctic Gram-negative bacterium, M. primoryensis, is mainly due to its highly repetitive RII (Region II). MpAFP_RII contains roughly 120 tandem copies of an identical 104-residue repeat. We have previously d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140083
更新日期:2014-07-04 00:00:00
abstract::Mitochondrial genome is a powerful molecule marker to provide information for phylogenetic relationships and revealing molecular evolution in ichthyological studies. Sebastiscus species, a marine rockfish, are of essential economic value. However, the taxonomic status and phylogenetic relationships of Sebastidae have ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20203379
更新日期:2020-12-23 00:00:00
abstract::Secretion of high molecular weight (HMW) adiponectin is dependent on post-translational modification (PTM) of conserved lysines in the collagenous domain. The present study aims to characterize the enzymes responsible for the PTM of conserved lysines which leads to HMW adiponectin secretion, and to define its signific...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170105
更新日期:2017-05-17 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disorder. However, studies providing PAH-related gene expression profiles are scarce. To identify hub genes involved in PAH, we investigate two microarray data sets from gene expression omnibus (GEO). A total of 150 differentially expr...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202346
更新日期:2020-09-30 00:00:00
abstract:AIM:IgA nephropathy (IgAN) is one of the most common chronic glomerulonephritis. Its etiology and pathogenesis remain unclear. We thus explored the immune repertoire of the B-cell receptor (BCR) and the heavy-chain complementarity-determining region 3 (CDR3) in renal tissue and peripheral blood of IgAN patients. METHO...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190482
更新日期:2019-10-30 00:00:00
abstract::The cellular onc-genes c-src and c-yes are expressed very differently during chicken embryonic development. The c-src mRNA and its translational product are detectable at high levels in brain extracts of chicken embryos and adult chickens, whereas muscle extracts show an age-dependent decrease in the amounts of c-src-...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128817
更新日期:1984-09-01 00:00:00
abstract::Two mouse monoclonal antibodies against the human complement control protein, Factor H (beta 1H), are described. The antibodies are both IgG - gamma 1 - subclass and are directed against different epitopes on the human Factor H molecule. One of the antibodies, MRC OX 24, increases the cofactor activity of Factor H in ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01120205
更新日期:1983-12-01 00:00:00
abstract::The first demonstration of a method to incorporate radiolabeled methyl groups from [14C]formaldehyde into membrane proteins in human erythrocytes by reductive methylation in the presence of NaCNBH3 is described. This method is based on the work of Jentoft and Dearborn [(1979) J. Biol. Chem. 254, 4359-4365], who demons...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136722
更新日期:1982-04-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a group of hereditary, degenerative retinal disorders characterized by progressive retinal dysfunction, outer retina cell loss, and retinal tissue atrophy. It eventually leads to tunnel vision and legal, or total blindness. Here we aimed to reveal the causal gene and mutation contributing ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171300
更新日期:2018-04-27 00:00:00
abstract::Acquired resistance of afatinib is a significant challenge for non-small cell lung cancer (NSCLC) therapy and the mechanisms remain unclear. Aberrant activation of epidermal growth factor receptor (EGFR)-dependent downstream pathways, especially phosphatidylinositol-3-kinases/protein kinase B (PI3K/Akt) signaling path...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181693
更新日期:2018-12-11 00:00:00
abstract::Breast cancer remains a leading cause of cancer-related mortality in women. In recent years, regulation of genes involved in heparan sulphate (HS) biosynthesis have received increased interest as regulators of breast cancer cell adhesion and invasion. The exostosin (EXT) proteins are glycosyltransferases involved in e...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180770
更新日期:2018-08-31 00:00:00
abstract::Tuberculosis (TB), a leading infectious disease caused by Mycobacterium tuberculosis strain, takes four human lives every minute globally. Paucity of knowledge on M. tuberculosis virulence and antibiotic resistance is the major challenge for tuberculosis control. We have identified 47 acetyltransferases in the M. tube...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191661
更新日期:2019-12-20 00:00:00
abstract::Mitochondria are known to participate in the initiation of programmed cell death (PCD) in animals and in plants. The role of chloroplasts in PCD is still unknown. We describe a new system to study PCD in plants; namely, leaf epidermal peels. The peel represents a monolayer consisting of cells of two types: phototrophi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1025576307912
更新日期:2003-04-01 00:00:00
abstract::Atherosclerosis is the primary cause of several cardiovascular diseases. Oxidized low-density lipoprotein (ox-LDL)-induced apoptosis, endothelial-mesenchymal transition (EndMT), and inflammation are crucial for the progression of cardiovascular diseases, including atherosclerosis. Naringin, a major compound from tomat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193431
更新日期:2020-03-27 00:00:00
abstract::The concentration of carbonic anhydrase III (CAIII) in male rat liver was found to be 30 times greater than that in the female. Castration of male rats led to marked reduction in liver CAIII concentrations which could be partially restored to control levels by testosterone replacement. Marked developmental and senesce...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121959
更新日期:1983-05-01 00:00:00