Abstract:
:Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and to study its biological functions in CRC progression. We focused on LncRNA DQ786243, one of LncRNAs which promoted development of CRC from the Gene Expression Omnibus (GEO) and validated using quantitative real-time PCR among about 20 paired CRC tissues. The effects of LncRNA DQ786243 were assessed by silencing the LncRNA in vitro and in vivo Results showed that the expression level LncRNA DQ786243 was significantly higher in CRC tissues and cell lines. We also found LncRNA DQ786243 knockdown by RNA interference with siRNA significantly arrested the cell cycle in the G2/M-phase, promoted apoptosis and weaken the abilities of cell proliferation and invasion in vitro Further investigation into the mechanisms responsible for the growth inhibitory effects by DQ786243 silencing revealed that its knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. Finally, xenograft experiments confirmed that the growth of xenograft tumours formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, the present study suggests that LncRNA DQ786243 is an oncogene that promotes tumour progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Sun L,Xue H,Jiang C,Zhou H,Gu L,Liu Y,Xu C,Xu Qdoi
10.1042/BSR20160048subject
Has Abstractpub_date
2016-05-06 00:00:00issue
3eissn
0144-8463issn
1573-4935pii
BSR20160048journal_volume
36pub_type
杂志文章abstract::Background: As one of the most common liver disorders worldwide, non-alcoholic fatty liver disease (NAFLD) begins with the abnormal accumulation of triglyceride (TG) in the liver. Long non-coding RNA-H19 was reported to modulate hepatic metabolic homeostasis in NAFLD. However, its molecular mechanism of NAFLD was not ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181722
更新日期:2019-07-15 00:00:00
abstract::The increase in % phosphorylase a associated with muscle contraction is reversed after a few minutes despite continued contractile activity. The present study was undertaken to determine whether adrenaline can activate phosphorylase after deactivation has occurred during prolonged stimulation of contraction. We found ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115118
更新日期:1982-05-01 00:00:00
abstract:OBJECTIVE:The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200487
更新日期:2020-08-28 00:00:00
abstract::More than 60 million people in the world have been diagnosed with HIV infections since the virus was recognized as the causative agent of AIDS in the 1980s. Even though more than half of the infected patients have died, effective disease treatment and prevention measures have not been established. ART (antiretroviral ...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20140097
更新日期:2014-08-18 00:00:00
abstract::This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119894
更新日期:1985-10-01 00:00:00
abstract::Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reporte...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192061
更新日期:2020-01-31 00:00:00
abstract::Ca-ATPase activity in sarcoplasmic reticulum (SR) membranes isolated from skeletal muscles of the typical hibernator, the ground squirrel Spermophilus undulatus, is about 2-fold lower than that in SR membranes of rats and rabbits and is further decreased 2-fold during hibernation. The use of carbocyanine anionic dye S...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1015540909212
更新日期:2001-12-01 00:00:00
abstract::Wilms tumor is the most common renal malignancy that occurs in children. TP53 gene is considered as a tumor-suppressing gene through controlling cell growth. TP53 gene rs1042522 C>G (Arg72Pro) polymorphism is widely investigated in various types of cancers. However, it is not established if TP53 rs1042522 C>G polymorp...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181891
更新日期:2019-01-22 00:00:00
abstract::Incubation of adult rat cardiac myocytes with increasing glucose concentrations decreased phosphorylation (αThr172) and activity of AMPK (AMP-activated protein kinase). The effect could be demonstrated without measurable changes in adenine nucleotide contents. The glucose effect was additive to the decrease in AMPK ac...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20110075
更新日期:2012-06-01 00:00:00
abstract::Extracts from uterine cervical and body cancers, but not from benign tumor or intact tissues tested, were found to contain a growth-promoting activity which induced the proliferation of human endometrial fibroblasts. Exposure of cultured fibroblasts to the cancer extracts increased the rate of [3H]thymidine incorporat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116850
更新日期:1990-02-01 00:00:00
abstract::Background: Selenoprotein S (SelS) gene expression is positively correlated to triglyceride (TG) concentrations and is associated with diabetes in animal model. However, the relationship between genetic polymorphisms of SelS and Type 2 diabetes (T2DM) remains unclear. Methods: In the present study, we genotyped four s...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181696
更新日期:2018-11-28 00:00:00
abstract::Esophageal cancer is seen with increasing incidence, but the underlying mechanism of esophageal cancer is still unknown. Transient receptor potential melastatin (TRPM) is non-selective cation channels. It has been verified that TRPM channels play crucial roles in development and progression of multiple tumors. Increas...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191878
更新日期:2019-10-30 00:00:00
abstract::Cervical carcinoma is the most common gynecological cancer in women worldwide. Emerging evidence has shown that long non-coding RNAs (lncRNAs) participate in multiple biological processes of cervical carcinoma tumorigenesis. We aimed to investigate the function of a novel lncRNA RP11-284F21.9 in cervical carcinoma. We...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200784
更新日期:2020-09-30 00:00:00
abstract::Mice and rats are animals commonly used in research and laboratory testing. Compared with other animal species, they harbor many more zoonotic agents. Hymenolepis nana (H. nana) is a common tapeworm that parasitizes both humans and rodents. Although this tapeworm is of socio-economic importance worldwide, information ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181224
更新日期:2019-02-08 00:00:00
abstract::CYP (cytochrome P450) 3A29 in pigs could be an important candidate gene responsible for xenobiotic metabolism, similar to CYP3A4 in humans. Accordingly, the tissue expression of CYP3A29 mRNA in domestic pigs has been determined by a real-time PCR. The enzymatic properties of CYP3A29, CYP3A4 and PLM (pig liver microsom...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20100084
更新日期:2011-06-01 00:00:00
abstract::The Gram-positive bacterium Staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. S. pseudintermedius also causes life-threatening human infections. Furthermore, methicillin-resistant S. pseudintermedius is emerging, resembling the human health threa...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140088
更新日期:2014-11-24 00:00:00
abstract::Artocarpus incisa (breadfruit) seeds contain three different lectins (Frutalin, Frutapin (FTP) and Frutackin) with distinct carbohydrate specificities. The most abundant lectin is Frutalin, an α-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and potential for tumour biomarker discove...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170969
更新日期:2017-07-21 00:00:00
abstract::Trypsin-releasable glycosaminoglycans from the luminal surface of intact pig aorta were measured following metabolic labelling with [35S]sulphate. Chondroitin sulphate was found to be present at a surface density equal to that already established for heparan sulphate (5 X 10(11) chains per cm2). This result was confir...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01124788
更新日期:1987-03-01 00:00:00
abstract::Permeant cationic fluorescent probes are widely employed to monitor mitochondrial transmembrane potential and its changes. The application of such potential-dependent probes in conjunction with both fluorescence microscopy and fluorescence spectroscopy allows the monitoring of mitochondrial membrane potential in indiv...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-007-9033-4
更新日期:2007-06-01 00:00:00
abstract::Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170302
更新日期:2017-07-20 00:00:00
abstract::Doxorubicin (DOX) resistance in breast cancer largely results from the breast cancer stem cell like cells (BCSCs) which could be targetted to improve the efficacy of chemotherapy. Cell permeabilization using microbubbles (MBs) and ultrasound (US) have the potential for delivering molecules into the cytoplasm. We aim t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171137
更新日期:2017-11-09 00:00:00
abstract::Hepatocellular carcinoma (HCC) has a high recurrence rate and poor clinical outcome after currently used therapies, including radiofrequency ablation. To explore the possible mechanisms for the relapse of HCC, in the present study we focussed on long non-coding RNA (LncRNA), which has been reported to be involved in t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180896
更新日期:2019-11-29 00:00:00
abstract::Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1010425932561
更新日期:2001-02-01 00:00:00
abstract::HIV-1 infects host cells by sequential interactions of its fusion protein (gp120-gp41) with receptors CD4, CXCR4 and/or CCR5 followed by fusion of viral and host membranes. Studies indicate that additional factors such as receptor density and composition of viral and cellular lipids can dramatically modulate the fusio...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-005-2894-5
更新日期:2005-10-01 00:00:00
abstract::To investigate whether the human anti-thrombospondin type 1 domain-containing 7A (THSD7A) antibody-induced membranous nephropathy (MN) is mediated by activating lectin complement pathway. Automatic biochemical apparatus was used to assess renal function of mice. The serum levels of anti-THSD7A antibodies and complemen...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180131
更新日期:2018-06-21 00:00:00
abstract::We assessed the relationship between the volume of epicardial adipose tissue and long-term outcomes in patients with coronary heart disease (CHD) undergoing percutaneous coronary intervention (PCI). The patients with CHD were followed for at least 2 years after PCI. The epicardial adipose tissue volume (EATV) was meas...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182278
更新日期:2019-05-07 00:00:00
abstract::Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [35S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins w...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117060
更新日期:1985-02-01 00:00:00
abstract::Background: Endometrial carcinoma (EC) still threatens the health of women. Thus, to explore how long intergenic non-protein coding RNA 01220 regulates the development of EC.Methods: Whole genome expression profile data of EC and paracancerous tissues in TCGA database were downloaded. LINC01220 expression in EC and pa...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181794
更新日期:2019-07-25 00:00:00
abstract::Imipramine and clorgyline, at concentrations of 0.002 M, inhibit the respiration of brain tissue by 82 and 71 per cent respectively, while chloropromazine and tranylcypromine, at concentrations of 0.01 M, inhibit it about 25 per cent. Deprenyl and amphetamine at a concentration of 0.002 M inhibit brain tissue respirat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01118600
更新日期:1991-02-01 00:00:00
abstract::Many clinical experiments and studies have demonstrated that traditional Chinese medicines possess the capacity for being used in anti-sepsis. In this paper, we screened 78 herbs based on biosensor technology by targeting of lipid A. Terminaliachebula Retz was found to possess the highest capability of binding lipid A...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20080158
更新日期:2009-10-06 00:00:00