Abstract:
INTRODUCTION:miRNAs dysregulate in spinal cord injury (SCI) and have been demonstrated to play a crucial role in neurite outgrowth. However, the underlying mechanism remains elusive. In this study, we constructed a mouse model of SCI, extracted RNA from injured spinal cord tissue for the use of microarray assay. miR-181d-5p which is one of the most significantly expressed miRNAs in miRNA-mRNA network, abundantly expressed in center system and highly conserved across different spices, was chosen for our further study. AIMS:To demonstrate whether miR-181d-5p can promote neurite outgrowth in PC12 cells via PI3K/Akt signaling pathway, we performed function analysis of miR-181d-5p with LV-miR-181d-5p and LV-sh-GFP to infect PC12 cells. RESULTS:Through microarray assay analysis, we totally found 262 significantly expressed miRNAs and 2973 target genes in SCI and observed that their expression dynamically changed postinjury. Here, we provided enough evidences that the overexpression of miR181d-5p significantly decreased the expression of PTEN, upregulated p-Akt expression, increased neurite outgrowth-related proteins (GAP-43 and NF-200) and synaptic vesicle-related proteins (Synapsin and PSD95), and then promoted neurite outgrowth in PC12 cells. Furthermore, we confirmed that miR-181d-5p could directly target to the 3'-UTR of PTEN mRNA through dual-luciferase report assay. CONCLUSIONS:Our study supports that aberrant expression of miRNAs is involved in the pathogenesis of SCI, miR-181d-5p plays an important role in neurite growth in PC12 cells via PI3K/Akt signaling pathway and may be a candidate target for the treatment of SCI in the future.
journal_name
CNS Neurosci Therjournal_title
CNS neuroscience & therapeuticsauthors
Shen LM,Song ZW,Hua Y,Chao X,Liu JBdoi
10.1111/cns.12761subject
Has Abstractpub_date
2017-11-01 00:00:00pages
894-906issue
11eissn
1755-5930issn
1755-5949journal_volume
23pub_type
杂志文章abstract::Stroke is a leading cause of death that affects 15 million people worldwide each year. Increasing evidence suggests that stroke confers substantial risk for suicide and following a stroke, patients frequently develop poststroke depression, which is a well-established risk factor for suicide. In this overview of the cu...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2012.00364.x
更新日期:2012-09-01 00:00:00
abstract::Understanding the neural substrates of depression is crucial for diagnosis and treatment. Here, we review recent studies of functional and effective connectivity in depression, in terms of functional integration in the brain. Findings from these studies, including our own, point to the involvement of at least four net...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/cns.12998
更新日期:2018-11-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12791
更新日期:2018-03-01 00:00:00
abstract::Octodon degus (O. degus) is a diurnal rodent that spontaneously develops several physiopathological conditions, analogous in many cases to those experienced by humans. In light of this, O. degus has recently been identified as a very valuable animal model for research in several medical fields, especially those concer...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/cns.12125
更新日期:2013-09-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13187
更新日期:2020-02-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12977
更新日期:2019-01-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2009.00105.x
更新日期:2009-01-01 00:00:00
abstract:AIMS:Blood-borne monocytes/macrophages infiltrate the brain in massive numbers after ischemic stroke, but their impact on poststroke brain injury and recovery remains elusive. This study examined the transcriptomic changes in monocytes/macrophages after ischemic stroke and the functional implications of these changes, ...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13256
更新日期:2019-12-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2009.00126.x
更新日期:2010-06-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13147
更新日期:2020-01-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,多中心研究
doi:10.1111/cns.13034
更新日期:2019-02-01 00:00:00
abstract:AIMS:Coagulation abnormality is one of the primary concerns for patients with spontaneous intracerebral hemorrhage admitted to ER. Conventional laboratory indicators require hours for coagulopathy diagnosis, which brings difficulties for appropriate intervention within the optimal window. This study evaluates the possi...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13509
更新日期:2020-11-28 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12274
更新日期:2014-06-01 00:00:00
abstract::Neurodegenerative diseases represent, nowadays, one of the main causes of death in the industrialized country. They are characterized by a loss of neurons in particular regions of the nervous system. It is believed that this nerve cell loss underlies the subsequent decline in cognitive and motor function that patients...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2008.00065.x
更新日期:2009-01-01 00:00:00
abstract::Ginsenosides are a special group of triterpenoid saponins attributed to medical effects of ginseng. Therefore, they have been research targets over the last three decades to explain ginseng actions and a wealth of literature has been presented reporting on ginsenosides' effects on the human body. Recently, there is in...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2010.00208.x
更新日期:2011-12-01 00:00:00
abstract::Tolerance development after successful long-term treatment of bipolar disorder is under recognized, as are ways to prevent or show its occurrence or reverse it once it has occurred. We review the clinical literature which suggests that tolerance can develop to most treatment approaches in bipolar illness and present a...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2010.00215.x
更新日期:2011-12-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13181
更新日期:2020-01-01 00:00:00
abstract::Alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) type glutamate receptors are critical for synaptic plasticity and induction of long-term potentiation (LTP), considered as one of the synaptic mechanisms underlying learning and memory. Positive allosteric modulators of AMPA receptors could provide a the...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/j.1755-5949.2009.00088.x
更新日期:2010-10-01 00:00:00
abstract::Tau, the microtubule-associated protein, forms insoluble filaments that accumulate as neurofibrillary tangles in Alzheimer's disease (AD) and related tauopathies. Under physiological conditions, tau regulates the assembly and maintenance of the structural stability of microtubules. In the diseased brain, however, tau ...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2010.00177.x
更新日期:2011-10-01 00:00:00
abstract:AIMS:To conduct a large-scale analysis on epidemiology, management, and outcomes of spontaneous subarachnoid hemorrhage (SAH), and to investigate the current situation of aneurysm obliteration in China. METHODS:A multicenter prospective cohort study involving 132 hospitals throughout China from September 2007 to Augus...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,多中心研究
doi:10.1111/cns.12001
更新日期:2012-11-01 00:00:00
abstract:AIMS:The 24-week, prospective, randomized, double-blind ACTION study investigated the efficacy, safety, and tolerability of 13.3 versus 4.6 mg/24 h rivastigmine patch in patients with severe Alzheimer's disease (AD). METHODS:Patients had probable AD and Mini-Mental State Examination scores ≥3-≤12. Primary outcome meas...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/cns.12158
更新日期:2013-10-01 00:00:00
abstract:AIMS:The mouse model of allo-head and body reconstruction (AHBR) has recently been established to further the clinical development of this strategy for patients who are suffering from mortal bodily trauma or disease, yet whose mind remains healthy. Animal model studies are indispensable for developing such novel surgic...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12422
更新日期:2015-08-01 00:00:00
abstract::The blood-brain barrier is a dynamic and complex neurovascular unit that protects neurons from somatic circulatory factors as well as regulates the internal environmental stability of the central nervous system. Endothelial glycocalyx is a critical component of an extended neurovascular unit that influences the struct...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13560
更新日期:2020-12-30 00:00:00
abstract:BACKGROUND:Activated microglia-mediated inflammation plays a key role in the pathogenesis of Alzheimer's disease (AD). In addition, chronic activation of NLRP3 inflammasomes triggered by amyloid β peptide (Aβ) in microglia contributes to persistent neuroinflammation. Here, the primary goal was to assess whether Dihydro...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12983
更新日期:2018-12-01 00:00:00
abstract:INTRODUCTION:Numerous genetic variants have been associated with susceptibility to multiple sclerosis (MS). Variants located in genes involved in specific pathways, such as those affecting TNF-α, can contribute to the risk of MS. The purpose of this study was to determine whether variants of these genes are associated ...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13456
更新日期:2020-11-01 00:00:00
abstract:AIMS:This study was designed to examine the neuroprotective effects of bakkenolide-IIIa, a major novel compound extracted from the rhizome of P. trichinous. METHODS:Transient focal cerebral damage model in rats and oxygen-glucose deprivation (OGD) in cultured hippocampal neurons were performed. The amount of apoptotic...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12470
更新日期:2015-12-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.13064
更新日期:2019-02-01 00:00:00
abstract::Cysteinyl leukotrienes are a group of the inflammatory lipid molecules well known as mediators of inflammatory signaling in the allergic diseases. Although they are traditionally known for their role in allergic asthma, allergic rhinitis, and others, recent advances in the field of biomedical research highlighted the ...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/cns.12596
更新日期:2016-12-01 00:00:00
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journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章
doi:10.1111/cns.12587
更新日期:2017-01-01 00:00:00
abstract::Appropriate and reliable classification of mental illness is crucial for advancing the field of psychiatry as agreement on diagnosis has broad implications for treatment of mental disorders and research into the etiopathophysiology of mental disorders. Since schizophrenia was first recognized by Kraepelin (as dementia...
journal_title:CNS neuroscience & therapeutics
pub_type: 杂志文章,评审
doi:10.1111/j.1755-5949.2010.00229.x
更新日期:2011-04-01 00:00:00