Abstract：
:In order to promote a local Tunisian product, this study was designed to examine, for the first time, the anti-Acanthamoeba activity (Acanthamoeba castellanii Neff) of the essential oils of Tunisian Citrus sinensis peels (Maltese half-blood) and the effect of viroid plant infection on this activity. To do so, three samples of peels' essential oils were studied: from a healthy plant (Control), a plant inoculated with Citrus exocortis viroid (CEVd) and one inoculated with hot stunt cachexia viroid (HSVd). The samples were extracted by hydrodistillation from dried peels and characterized by GC-MS. Limonene was the major component with a percentage ranging from 90.76 to 93.34% for (CEVd) sample and (Control), respectively. Anti-Acanthamoeba activity of the tested oils was determined by the Alamar Blue® assay. Primary results showed a strong potential anti-Acanthamoeba activity with an IC50 ranging from 36.6 to 54.58 μg/ml for (HSVd) and (CEVd) samples, respectively. In terms of the effect of viroid infection, a strong positive correlation was observed between different chemical classes and anti-Acanthamoeba activity.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Zouaghi G,Najar A,Chiboub O,Sifaoui I,Abderrabba M,Lorenzo Morales Jdoi
10.1016/j.exppara.2017.09.006subject
Has Abstractpub_date
2017-12-01 00:00:00pages
182-186eissn
0014-4894issn
1090-2449pii
S0014-4894(17)30415-0journal_volume
183pub_type
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