Abstract:
:Classical swine fever virus (CSFV) non-structural protein 3 (NS3) is a multifunctional non-structural protein that plays a major role in viral replication. However, how exactly NS3 exerts these functions remains unknown. Here, we identified tumour necrosis factor receptor-associated factor 6 (TRAF6) as a novel NS3-interacting protein via yeast two-hybrid analysis, co-immunoprecipitation, and glutathione S-transferase pull-down assays. Furthermore, we observed that TRAF6 overexpression significantly inhibited CSFV replication, and TRAF6 knockdown promoted CSFV replication in porcine alveolar macrophages. Additionally, TRAF6 was degraded during CSFV infection or NS3 expression exclusively, indicating that CSFV and TRAF6 were mutually antagonistic and that TRAF6 degradation might contribute to persistent CSFV replication. Moreover, nuclear factor-kappa B (NF-κB) activity and interferon (IFN)-β and interleukin (IL)-6 expression were increased in TRAF6-overexpressing cells, whereas TRAF6-knockdown cells exhibited decreased NF-κB activity and IFN-β and IL-6 levels. Notably, TRAF6 overexpression did not reduce CSFV replication following inhibition of NF-κB activation by p65 knockdown. Our findings revealed that TRAF6 inhibits CSFV replication via activation of NF-κB-signalling pathways along with increases in the expression of its targets IFN-β and IL-6. This work addresses a novel aspect concerning the regulation of innate antiviral immune response during CSFV infection.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lv H,Dong W,Cao Z,Li X,Wang J,Qian G,Lv Q,Wang C,Guo K,Zhang Ydoi
10.1038/s41598-017-06934-1subject
Has Abstractpub_date
2017-07-27 00:00:00pages
6737issue
1issn
2045-2322pii
10.1038/s41598-017-06934-1journal_volume
7pub_type
杂志文章abstract::Assassin bugs (Reduvioidea) are one of the most diverse (>7,000 spp.) lineages of predatory animals and have evolved an astounding diversity of raptorial leg modifications for handling prey. The evolution of these modifications is not well understood due to the lack of a robust phylogeny, especially at deeper nodes. W...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22177
更新日期:2016-02-26 00:00:00
abstract::Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that are related to inf...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00347
更新日期:2012-01-01 00:00:00
abstract::Hand preference is a conspicuous variation in human behaviour, with a worldwide proportion of around 90% of people preferring to use the right hand for many tasks, and 10% the left hand. We used the large cohort of the UK biobank (~500,000 participants) to study possible relations between early life factors and adult ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37423-8
更新日期:2019-01-24 00:00:00
abstract::The study of organic/inorganic molecules with activity against intracellular fungi of the phylum Microsporidia is of critical importance. Here, for the first time, the inactivation of these parasitic fungi by porphyrins is reported. The biological effects of porphyrins (10 µM and 100 µM) on the microsporidian Nosema c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23678-8
更新日期:2018-04-03 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-43786-3
更新日期:2019-10-28 00:00:00
abstract::Pulse wave transit time (PWTT) has been widely used as an index in assessing arterial stiffness. Percutaneous coronary intervention (PCI) is usually applied to the treatment of coronary artery disease (CAD). Research on the changes in PWTT caused by PCI is helpful for understanding the impact of the PCI procedure. In ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18520-6
更新日期:2018-01-08 00:00:00
abstract::This work presents the measurement of the permittivities of water and methanol in the D-band. Water is a reference medium for dielectric measurement. The dielectric permittivity of water in the millimeter wave range is a fundamental parameter in many applications, and needs to be investigated systematically. The measu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55126-6
更新日期:2019-12-06 00:00:00
abstract::De novo protein sequencing is essential for understanding cellular processes that govern the function of living organisms and all sequence modifications that occur after a protein has been constructed from its corresponding DNA code. By obtaining the order of the amino acids that compose a given protein one can then d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25232
更新日期:2016-05-03 00:00:00
abstract::Studies of morphological integration and modularity, and of anatomical complexity in human evolution typically focus on skeletal tissues. Here we provide the first network analysis of the musculoskeletal anatomy of both the fore- and hindlimbs of the two species of chimpanzee and humans. Contra long-accepted ideas, ne...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25262-6
更新日期:2018-05-02 00:00:00
abstract::A remarkable feature of vanadium dioxide is that it can be synthesized in a number of polymorphs. The conductivity mechanism in the metastable layered polymorph VO2(B) thin films has been investigated by terahertz time-domain spectroscopy (THz-TDS). In VO2(B), a critical temperature of 240 K marks the appearance of a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09182
更新日期:2015-03-17 00:00:00
abstract::SerpinB3 has been recently described as an early marker of liver carcinogenesis, but the potential mechanistic role of this serpin in tumor development is still poorly understood. Overexpression of Myc often correlates with more aggressive tumour forms, supporting its involvement in carcinogenesis. Yes-associated prot...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17701
更新日期:2015-12-04 00:00:00
abstract::The double-slit experiment strikingly demonstrates the wave-particle duality of quantum objects. In this famous experiment, particles pass one-by-one through a pair of slits and are detected on a distant screen. A distinct wave-like pattern emerges after many discrete particle impacts as if each particle is passing th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04685
更新日期:2014-04-28 00:00:00
abstract::Recruitment is one of the dominant processes regulating fish population productivity. It is, however, notoriously difficult to predict, as it is the result of a complex multi-step process. Various fine-scale drivers might act on the pathway from adult population characteristics to spawning behaviour and egg production...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-020-73025-z
更新日期:2020-09-30 00:00:00
abstract::Loss of miR-200c is correlated to advanced cancer-subtypes due to increased EMT and decreased treatment efficacy by chemotherapeutics. As miRNAs regulate a multitude of targets, the analysis of differentially expressed proteins upon a genomic knock-out (KO) is of interest. In this study, we generated a TALENs KO of mi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25240-y
更新日期:2018-05-02 00:00:00
abstract::Dissection of the neural circuits of the cerebral cortex is essential for studying mechanisms underlying brain function. Herein, combining a retrograde rabies tracing system with fluorescent micro-optical sectional tomography, we investigated long-range input neurons of corticotropin-releasing hormone containing neuro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68115-x
更新日期:2020-07-22 00:00:00
abstract::Developing effective treatment strategies for neurodegenerative diseases require an understanding of the underlying cellular pathways that lead to neuronal vulnerability and progressive degeneration. To date, numerous mutations in 147 distinct genes are identified to be "associated" with, "modifier" or "causative" of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32902-4
更新日期:2018-10-03 00:00:00
abstract::Primordial germ cells (PGCs), the embryonic precursors of the sperm and egg, are used for the introduction of genetic modifications into avian genome. Introduction of small defined sequences using genome editing has not been demonstrated in bird species. Here, we compared oligonucleotide-mediated HDR using wild type S...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33244-x
更新日期:2018-10-11 00:00:00
abstract::Successful tick feeding is facilitated by an assortment of pharmacologically-active factors in tick saliva that create an immunologically privileged micro-environment in the host's skin. Through a process known as saliva-assisted transmission, bioactive tick salivary factors modulate the host environment, promoting tr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49572-5
更新日期:2019-09-11 00:00:00
abstract::Macro- and micro-environmental factors modulate parasite loads in fish, determining parasitic abundances, diversity, and interaction dynamics. In this study, seasonal variations in larval ectoparasites on fish larvae in the northern Humboldt Current System (HCS) were evaluated using a delta-gamma generalized linear mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79847-1
更新日期:2021-01-12 00:00:00
abstract::Growing evidence indicates that nomogram combined with the biomarkers of systemic inflammation response could provide more accurate prediction than conventional staging systems in tumors. This study aimed to establish an effective prognostic nomogram for resectable thoracic esophageal squamous cell carcinoma (ESCC) ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18811
更新日期:2015-12-22 00:00:00
abstract::Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory functi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29695
更新日期:2016-07-21 00:00:00
abstract::One mechanism of action for the anticaries effect of topical fluoridation is through precipitation of CaF2. In this in vitro study energy-dispersive x-ray spectroscopy (EDX) is used as a semiquantitative method to detect enamel fluoride-precipitation under the influence of acidic and neutral pH-value and absence or pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49742-5
更新日期:2019-09-17 00:00:00
abstract::Balancing the amounts of enzymes is one of the important factors to achieve optimum performance of a designed metabolic pathway. However, the random mutagenesis approach is impractical since it requires searching an unnecessarily large number of variants and often results in searching a narrow range of expression leve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04515
更新日期:2014-03-31 00:00:00
abstract::Few neuroimaigng studies on reading comprehension have been conducted under natural reading settings. In this study, we showed texts presented in a natural way during functional MRI (fMRI) measurements to reveal brain areas sensitive to reading comprehension. Specifically, this paradigm independently manipulated two h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49632-w
更新日期:2019-09-13 00:00:00
abstract::Environmental cycles often influence the presence of animals, creating patterns at different temporal scales, which may mean that their effects overlap and/or interact. Interactions between diel and seasonal cycles have been reported to influence fish behaviour but little is known about such interactions in marine top...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38900-4
更新日期:2019-02-22 00:00:00
abstract::It has been a key issue for photovoltaic (PV) cells to survive under mechanical impacts by tiny dust. In this paper, the performance degradation and the damage behavior of PV cells subjected to massive dust impact are investigated using laser-shock driven particle impact experiments and mechanical modeling. The result...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80879-w
更新日期:2021-01-12 00:00:00
abstract::It has been suggested that orthographic transparency and age changes may affect the relationship between visual attention span (VAS) deficit and reading difficulty. The present study explored the developmental trend of VAS in children with developmental dyslexia (DD) in Chinese, a logographic language with a deep orth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21578-5
更新日期:2018-02-16 00:00:00
abstract::We propose a deep learning model - Probabilistic Prognostic Estimates of Survival in Metastatic Cancer Patients (PPES-Met) for estimating short-term life expectancy (>3 months) of the patients by analyzing free-text clinical notes in the electronic medical record, while maintaining the temporal visit sequence. In a si...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27946-5
更新日期:2018-07-03 00:00:00
abstract::Methicillin resistant Staphylococcus aureus (MRSA) is a major human pathogen, which causes superficial to lethal clinical infections. Neutrophils are the most abundant leukocytes in the blood and are the first defense mechanism against S. aureus infections. Here we show Staphylococcal Superantigen-Like protein 11 (SSL...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40817-x
更新日期:2019-03-12 00:00:00
abstract::The access and recycling of the base cations are essential processes for the long-lasting functioning of forest ecosystems. While the role of soil bacterial communities has been demonstrated in mineral weathering and tree nutrition, our understanding of the link between the availability of base cations and the functio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50730-y
更新日期:2019-10-07 00:00:00